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Cited by 77T-cell and Retrovirus StudiesVirus-based gene therapy researchHIV Research and Treatment

Relationship of avian retrovirus DNA synthesis to integration in vitro.

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Lee, Y M, Coffin, J M·1991·Molecular and Cellular Biology
DOI 10.1128/mcb.11.3.1419PMC PMC369417
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Abstract

An in vitro integration system derived from avian leukosis virus-infected cells supports both intra- and intermolecular integration of the viral DNA. In the absence of polyethylene glycol, intramolecular integration of viral DNA molecules into themselves (autointegration) was preferred. In the presence of polyethylene glycol, integration into an exogenously supplied DNA target was greatly promoted. Analysis of integration intermediates revealed that the strand transfer mechanisms of both reactions were identical to those of retroviruses and some transposons: each 3' end of the donor molecule is joined to a 5' end of the cleaved target DNA. The immediate integration precursor appears to be linear viral DNA with the 3' ends shortened by 2 nucleotides. Finally, in the avian system, most cytoplasmic viral DNA appears to be incomplete and further DNA synthesis is required for integration in vitro.

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  • Retroviral reverse transcription proceeds through steps including initiation of minus-strand DNA synthesis using tRNA primer, minus-strand strong stop synthesis, RNase H removal, first template switching, completion of minus-strand synthesis and RNA degradation, PPT primer for plus-strand synthesis, second template switching, and completion of dsDNA with two complete flanking LTRs.

    “The various steps involved in retroviral reverse transcription are illustrated in Fig. 6, and include: (i) initiation of minus strand DNA synthesis using tRNA as a primer, (ii) synthesis of minus strand strong stop DNA, (iii) removal of the RNA part of the RNA–DNA hybrid by RNase H activity, (iv) the first template switching or negative DNA strand transfer using the R regions at the two flanks, (v”

    From Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication (2026) · cites this paper as Lee and Coffin 1991

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Research works printed but not linked: 26

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Cited by

Sorted by citations

Authors
Lee, Y. M., & Coffin, J. M.
Affiliations
Tufts University (US)
ORCID
Coffin, J M
Venue
Molecular and Cellular Biology
Publisher
Informa UK Limited
Published
1991-03
Volume
11
Issue
3
Pages
1419-1430
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
DOI
10.1128/mcb.11.3.1419
PMC
PMC369417
OpenAlex
W2161266753
Cited by
77
References
6
Citations by year
2025: 1 · 2022: 1 · 2020: 2 · 2017: 1 · 2015: 4 · 2014: 4
FWCI
1.04
Type
journal article
Language
en
Open access
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r35ca044385-14, 5r01ca017659-11)
  • National Cancer Institute (CA17659, CA44385)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
  • Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
Keywords
avian retroviruses, avian leukosis virus, viral DNA integration, DNA synthesis, polyethylene glycol, strand transfer, transposable elements
MeSH terms
Avian Leukosis Virus, Base Sequence, Cell Transformation, Viral, DNA, Viral, Molecular Sequence Data, Polyethylene Glycols, Recombination, Genetic, Virus Replication, Blotting, Southern, Restriction Mapping, Dideoxynucleosides, RNA Probes, In Vitro Techniques

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