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Cited by 199Telomeres, Telomerase, and SenescenceGenetics, Aging, and Longevity in Model OrganismsNuclear Structure and Function

The DOI matches its Crossref record; 87 of the 88 references it cites were found in a registry or an index.

Diverse gene sequences are overexpressed in werner syndrome fibroblasts undergoing premature replicative senescence.

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Murano, S, Thweatt, R, Shmookler Reis, R J, Jones, R A, Moerman, E J, Goldstein, S·1991·Molecular and Cellular Biology
DOI 10.1128/mcb.11.8.3905PMC PMC361182
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Abstract

Genes that play a role in the senescent arrest of cellular replication are likely to be overexpressed in human diploid fibroblasts (HDF) derived from subjects with Werner syndrome (WS) because these cells have a severely curtailed replicative life span. To identify some of these genes, a cDNA library was constructed from WS HDF after they had been serum depleted and repleted (5 days in medium containing 1% serum followed by 24 h in medium containing 20% serum). Differential screening of 7,500 colonies revealed 102 clones that hybridized preferentially with [32P]cDNA derived from RNA of WS cells compared with [32P]cDNA derived from normal HDF. Cross-hybridization and partial DNA sequence determination identified 18 independent gene sequences, 9 of them known and 9 unknown. The known genes included alpha 1(I) procollagen, alpha 2(I) procollagen, fibronectin, ferritin heavy chain, insulinlike growth factor-binding protein-3 (IGFBP-3), osteonectin, human tissue plasminogen activator inhibitor type I, thrombospondin, and alpha B-crystallin. The nine unknown clones included two novel gene sequences and seven additional sequences that contained both novel segments and the Alu class of repetitive short interspersed nuclear elements; five of these seven Alu+ clones also contained the long interpersed nuclear element I (KpnI) family of repetitive elements. Northern (RNA) analysis, using the 18 sequences as probes, showed higher levels of these mRNAs in WS HDF than in normal HDF. Five selected mRNAs studied in greater detail [alpha 1(I) procollagen, fibronectin, insulinlike growth factor-binding protein-3, WS3-10, and WS9-14] showed higher mRNA levels in both WS and late-passage normal HDF than in early-passage normal HDF at various intervals following serum depletion/repletion and after subculture and growth from sparse to high-density confluent arrest. These results indicate that senescence of both WS and normal HDF is accompanied by overexpression of similar sets of diverse genes which may play a role in the senescent arrest of cellular replication and in the genesis of WS, normal biological aging, and attendant diseases.

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Metadata

Authors
Murano, S., Thweatt, R., Shmookler Reis, R. J., Jones, R. A., Moerman, E. J., & Goldstein, S.
Affiliations
University of Arkansas for Medical Sciences (US)
ORCID
Goldstein, S
Venue
Molecular and Cellular Biology
Publisher
Informa UK Limited
Published
1991-08
Volume
11
Issue
8
Pages
3905-3914
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
DOI
10.1128/mcb.11.8.3905
PMC
PMC361182
OpenAlex
W2150967453
Cited by
199
References
88
Citations by year
2023: 3 · 2022: 1 · 2021: 5 · 2020: 5 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 4 · 2013: 1 · 2012: 3
FWCI
14.45
Type
journal article
Language
en
Open access
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • University of Arkansas
  • Arkansas Science and Technology Authority
  • University of Arkansas for Medical Sciences
  • National Institutes of Health (5r01ag008708-05)
  • National Institute on Aging (AG08708)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
Werner syndrome, human diploid fibroblasts, novel gene sequences, Alu repetitive elements, long interspersed nuclear elements, fibronectin, insulin-like growth factor binding protein-3, thrombospondin, alphaB-crystallin, biological aging
MeSH terms
Base Sequence, Cell Division, Cells, Cultured, DNA, Fibroblasts, Genes, Humans, Molecular Sequence Data, Proteins, RNA, RNA, Messenger, Skin, Werner Syndrome, Blotting, Northern, Oligonucleotide Probes, Gene Library, Gene Expression

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