CoChat
Sign Up
Cited by 199Telomeres, Telomerase, and SenescenceGenetics, Aging, and Longevity in Model OrganismsNuclear Structure and Function

The DOI matches its Crossref record; 87 of the 88 references it cites were found in a registry or an index.

Diverse gene sequences are overexpressed in werner syndrome fibroblasts undergoing premature replicative senescence.

Open access
Murano, S, Thweatt, R, Shmookler Reis, R J, Jones, R A, Moerman, E J, Goldstein, S·1991·Molecular and Cellular Biology
DOI 10.1128/mcb.11.8.3905PMC PMC361182
Open this paper in CoChat — free account

Abstract

Genes that play a role in the senescent arrest of cellular replication are likely to be overexpressed in human diploid fibroblasts (HDF) derived from subjects with Werner syndrome (WS) because these cells have a severely curtailed replicative life span. To identify some of these genes, a cDNA library was constructed from WS HDF after they had been serum depleted and repleted (5 days in medium containing 1% serum followed by 24 h in medium containing 20% serum). Differential screening of 7,500 colonies revealed 102 clones that hybridized preferentially with [32P]cDNA derived from RNA of WS cells compared with [32P]cDNA derived from normal HDF. Cross-hybridization and partial DNA sequence determination identified 18 independent gene sequences, 9 of them known and 9 unknown. The known genes included alpha 1(I) procollagen, alpha 2(I) procollagen, fibronectin, ferritin heavy chain, insulinlike growth factor-binding protein-3 (IGFBP-3), osteonectin, human tissue plasminogen activator inhibitor type I, thrombospondin, and alpha B-crystallin. The nine unknown clones included two novel gene sequences and seven additional sequences that contained both novel segments and the Alu class of repetitive short interspersed nuclear elements; five of these seven Alu+ clones also contained the long interpersed nuclear element I (KpnI) family of repetitive elements. Northern (RNA) analysis, using the 18 sequences as probes, showed higher levels of these mRNAs in WS HDF than in normal HDF. Five selected mRNAs studied in greater detail [alpha 1(I) procollagen, fibronectin, insulinlike growth factor-binding protein-3, WS3-10, and WS9-14] showed higher mRNA levels in both WS and late-passage normal HDF than in early-passage normal HDF at various intervals following serum depletion/repletion and after subculture and growth from sparse to high-density confluent arrest. These results indicate that senescence of both WS and normal HDF is accompanied by overexpression of similar sets of diverse genes which may play a role in the senescent arrest of cellular replication and in the genesis of WS, normal biological aging, and attendant diseases.

Overview

Integrity signals

Registry

Crossref · 10.1128/mcb.11.8.3905

Cites

87 of 87 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 88 references

Cited by

Sorted by citations

Related

See all

Metadata

Authors
Murano, S., Thweatt, R., Shmookler Reis, R. J., Jones, R. A., Moerman, E. J., & Goldstein, S.
Affiliations
University of Arkansas for Medical Sciences (US)
ORCID
Goldstein, S
Venue
Molecular and Cellular Biology
Publisher
Informa UK Limited
Published
1991-08
Volume
11
Issue
8
Pages
3905-3914
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
DOI
10.1128/mcb.11.8.3905
PMC
PMC361182
OpenAlex
W2150967453
Cited by
199
References
88
Citations by year
2023: 3 · 2022: 1 · 2021: 5 · 2020: 5 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 4 · 2013: 1 · 2012: 3
FWCI
14.45
Type
journal article
Language
en
Open access
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • University of Arkansas
  • Arkansas Science and Technology Authority
  • University of Arkansas for Medical Sciences
  • National Institutes of Health (5r01ag008708-05)
  • National Institute on Aging (AG08708)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
Werner syndrome, human diploid fibroblasts, novel gene sequences, Alu repetitive elements, long interspersed nuclear elements, fibronectin, insulin-like growth factor binding protein-3, thrombospondin, alphaB-crystallin, biological aging
MeSH terms
Base Sequence, Cell Division, Cells, Cultured, DNA, Fibroblasts, Genes, Humans, Molecular Sequence Data, Proteins, RNA, RNA, Messenger, Skin, Werner Syndrome, Blotting, Northern, Oligonucleotide Probes, Gene Library, Gene Expression

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review