The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Complementation of a capsule-deficient mutation of Cryptococcus neoformans restores its virulence.
Open accessAbstract
Capsule formation plays a significant role in the pathogenicity of Cryptococcus neoformans. To study the molecular basis of capsule synthesis, the capsule-deficient phenotype of a mutant strain was complemented by transformation. A plasmid rescued from the resulting Cap+ transformant complemented a cap59 mutation which was mapped previously by classical recombination analysis. Gene deletion by homologous integration resulted in an acapsular phenotype, indicating that we have identified the CAP59 gene. The CAP59 gene was assigned to chromosome I by Southern blot analysis of contour-clamped homogeneous electric field gel electrophoresis-resolved chromosomes of C. neoformans var. neoformans. Sequence comparison of genomic and cDNA clones indicated the presence of six introns. CAP59 encoded a 1.9-kb transcript and a deduced protein of 458 amino acids. Analysis of the nucleotide sequence revealed little similarity to existing sequences in the data bank. When the capsule-deficient phenotype was complemented, the originally avirulent C. neoformans strain became virulent for mice. In addition, the acapsular strain created by gene deletion of CAP59 lost its virulence. This work demonstrates the molecular basis for capsule-related virulence and that the CAP59 gene is required for capsule formation.
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Cites
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
Feinberg, A. P., & Vogelstein, B. (1983) · Analytical Biochemistry · doi:10.1016/0003-2697(83)90418-9 · Cited by 24,887
A comprehensive set of sequence analysis programs for the VAX
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Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA
Toffaletti, D. L., Rude, T. H., Johnston, S. A., Durack, D. T., & Perfect, J. R. (1993) · Journal of Bacteriology · doi:10.1128/jb.175.5.1405-1411.1993 · Cited by 488
Genetic association of mating types and virulence in Cryptococcus neoformans
Kwon-Chung, K. J., Edman, J. C., & Wickes, B. L. (1992) · Infection and Immunity · doi:10.1128/iai.60.2.602-605.1992 · Cited by 434
Self-citationMorphogenesis of Filobasidiella neoformans, the Sexual State of Cryptococcus neoformans
Kwon-Chung, K. J. (1976) · Mycologia · doi:10.2307/3758800 · Cited by 352
Self-citationEncapsulation and melanin formation as indicators of virulence in Cryptococcus neoformans
Kwon-Chung, K. J., & Rhodes, J. C. (1986) · Infection and Immunity · doi:10.1128/iai.51.1.218-223.1986 · Cited by 341
Self-citationVirulence of Cryptococcus neoformans. Regulation of capsule synthesis by carbon dioxide.
Granger, D. L., Perfect, J. R., & Durack, D. T. (1985) · Journal of Clinical Investigation · doi:10.1172/jci112000 · Cited by 319
Decreased virulence in stable, acapsular mutants ofCryptococcus neoformans
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Self-citationCapsular Polysaccharides of Cryptococcus neoformans
Bhattacharjee, A. K., Bennett, J. E., & Glaudemans, C. P. J. (1984) · Clinical Infectious Diseases · doi:10.1093/clinids/6.5.619 · Cited by 192
Cited by
Cryptococcosis in the era of AIDS--100 years after the discovery of Cryptococcus neoformans
Mitchell, T. G., & Perfect, J. R. (1995) · Clinical Microbiology Reviews · doi:10.1128/cmr.8.4.515 · Cited by 1,213
Calcineurin is required for virulence of Cryptococcus neoformans
Odom, A., Muir, S., Lim, E., Toffaletti, D. L., Perfect, J., & Heitman, J. (1997) · The EMBO Journal · doi:10.1093/emboj/16.10.2576 · Cited by 516
Cryptococcus neoformans and Cryptococcus gattii, the etiologic agents of cryptococcosis
Kwon-Chung, K. J., Fraser, J. A., Doering, T. L., Wang, Z. A., Janbon, G., Idnurm, A., & Bahn, Y.-S. (2014) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a019760 · Cited by 531
Self-citationUrease as a Virulence Factor in Experimental Cryptococcosis
Cox, G. M., Mukherjee, J., Cole, G. T., Casadevall, A., & Perfect, J. R. (2000) · Infection and Immunity · doi:10.1128/iai.68.2.443-448.2000 · Cited by 522
Antifungal agents: chemotherapeutic targets and immunologic strategies
Georgopapadakou, N. H., & Walsh, T. J. (1996) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.40.2.279 · Cited by 498
Metadata
- Authors
- Chang, Y. C., & Kwon-Chung, K. J.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1994-07
- Volume
- 14
- Issue
- 7
- Pages
- 4912-4919
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2096855445
- Cited by
- 490
- References
- 24
- FWCI
- 7.54
- Type
- journal article
- Language
- en
- Open access
- green
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- Chang, Y. C., & Kwon-Chung, K. J. (1994). Complementation of a capsule-deficient mutation of Cryptococcus neoformans restores its virulence. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.14.7.4912
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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