The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation.
Open accessAbstract
A cDNA encoding Cryptococcus neoformans orotidine monophosphate pyrophosphorylase (OMPPase) has been isolated by complementation of the cognate Escherichia coli pyrE mutant. The cDNA was used as a probe to isolate a genomic DNA fragment encoding the OMPPase gene (URA5). By using electroporation for the introduction of plasmid DNA containing the URA5 gene, C. neoformans ura5 mutants could be transformed at low efficiency. Ura+ transformants obtained with supercoiled plasmids containing the URA5 gene showed marked mitotic instability and contained extrachromosomal URA5 sequences, suggesting limited ability to replicate within C. neoformans. Transformants obtained with linear DNA were of two classes: stable transformants with integrated URA5 sequences, and unstable transformants with extrachromosomal URA5 sequences.
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Citations
Cites
Melanin-lacking mutants of Cryptococcus neoformans and their virulence for mice
Kwon-Chung, K. J., Polacheck, I., & Popkin, T. J. (1982) · Journal of Bacteriology · doi:10.1128/jb.150.3.1414-1421.1982 · Cited by 327
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Cited by
Cryptococcosis in the era of AIDS--100 years after the discovery of Cryptococcus neoformans
Mitchell, T. G., & Perfect, J. R. (1995) · Clinical Microbiology Reviews · doi:10.1128/cmr.8.4.515 · Cited by 1,213
Signal Transduction Cascades Regulating Fungal Development and Virulence
Lengeler, K. B., Davidson, R. C., D'souza, C., Harashima, T., Shen, W.-C., Wang, P., Pan, X., Waugh, M., & Heitman, J. (2000) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.64.4.746-785.2000 · Cited by 915
Cryptococcus neoformans and Cryptococcus gattii, the etiologic agents of cryptococcosis
Kwon-Chung, K. J., Fraser, J. A., Doering, T. L., Wang, Z. A., Janbon, G., Idnurm, A., & Bahn, Y.-S. (2014) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a019760 · Cited by 531
Self-citationComplementation of a capsule-deficient mutation of Cryptococcus neoformans restores its virulence.
Chang, Y. C., & Kwon-Chung, K. J. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.7.4912 · Cited by 490
Self-citationA PCR-based strategy to generate integrative targeting alleles with large regions of homology
Davidson, R. C., Blankenship, J. R., Kraus, P. R., de Jesus Berrios, M., Hull, C. M., D’Souza, C., Wang, P., & Heitman, J. (2002) · Microbiology · doi:10.1099/00221287-148-8-2607 · Cited by 299
Metadata
- Authors
- Edman, J. C., & Kwon-Chung, K. J.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1990-09
- Volume
- 10
- Issue
- 9
- Pages
- 4538-4544
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2124590513
- Cited by
- 196
- References
- 1
- FWCI
- 2.57
- Type
- journal article
- Language
- en
- Open access
- green
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- Edman, J. C., & Kwon-Chung, K. J. (1990). Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.10.9.4538
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