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The t(8;21) Fusion Protein Interferes with AML-1B-Dependent Transcriptional Activation
Open accessAbstract
The AML-1/CBF beta transcription factor complex is targeted by both the t(8;21) and the inv(16) chromosomal alterations, which are frequently observed in acute myelogenous leukemia. AML-1 is a site-specific DNA-binding protein that recognizes the enhancer core motif TGTGGT. The t(8;21) translocation fuses the first 177 amino acids of AML-1 to MTG8 (also known as ETO), generating a chimeric protein that retains the DNA-binding domain of AML-1. Analysis of endogenous AML-1 DNA-binding complexes suggested the presence of at least two AML-1 isoforms. Accordingly, we screened a human B-cell cDNA library and isolated a larger, potentially alternatively spliced, form of AML1, termed AML1B. AML-1B is a protein of 53 kDa that binds to a consensus AML-1-binding site and complexes with CBF beta. Subcellular fractionation experiments demonstrated that both AML-1 and AML-1/ETO are efficiently extracted from the nucleus under ionic conditions but that AML-1B is localized to a salt-resistant nuclear compartment. Analysis of the transcriptional activities of AML-1, AML-1B, and AML-1/ETO demonstrated that only AML-1B activates transcription from the T-cell receptor beta enhancer. Mixing experiments indicated that AML-1/ETO can efficiently block AML-1B-dependent transcriptional activation, suggesting that the t(8;21) translocation creates a dominant interfering protein.
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Dignam, J. D., Lebovitz, R. M., & Roeder, R. G. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.5.1475 · Cited by 11,262
Recombinant Genomes Which Express Chloramphenicol Acetyltransferase in Mammalian Cells
Gorman, C., Moffat, L. F., & Howard, B. H. (1982) · Molecular and Cellular Biology · doi:10.1128/mcb.2.9.1044 · Cited by 7,674
The scanning model for translation: an update.
Kozak, M. (1989) · The Journal of Cell Biology · doi:10.1083/jcb.108.2.229 · Cited by 3,485
Miyoshi, H., Shimizu, K., Kozu, T., Maseki, N., Kaneko, Y., & Ohki, M. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.23.10431 · Cited by 872
Multiple Point Mutations Affecting the Simian Virus 40 Enhancer
Weiher, H., König, M., & Gruß, P. (1983) · Science · doi:10.1126/science.6297005 · Cited by 803
Fusion Between Transcription Factor CBFβ/PEBP2β and a Myosin Heavy Chain in Acute Myeloid Leukemia
Liu, P., Tarlé, S. A., Hajra, A., Claxton, D. F., Marlton, P., Freedman, M., Siciliano, M. J., & Collins, F. S. (1993) · Science · doi:10.1126/science.8351518 · Cited by 732
Berger, J., Hauber, J., Hauber, R., Geiger, R. E., & Cullen, B. R. (1988) · Gene · doi:10.1016/0378-1119(88)90219-3 · Cited by 667
Erickson, P., Gao, J., Chang, K., Look, T., Whisenant, E., Raimondi, S., Lasher, R., Trujillo, J., Rowley, J., & Drabkin, H. (1992) · Blood · doi:10.1182/blood.v80.7.1825.1825 · Cited by 631
Ogawa, E., Maruyama, M., Kagoshima, H., Inuzuka, M., Lu, J., Satake, M., Shigesada, K., & Ito, Y. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.14.6859 · Cited by 608
The interaction of RB with E2F coincides with an inhibition of the transcriptional activity of E2F.Preprint
Hiebert, S. W., Chellappan, S., Horowitz, J. M., & Nevins, J. R. (1992) · Genes & Development · doi:10.1101/gad.6.2.177 · Cited by 576
Self-citation
Research works printed but not linked: 5
- Bae S.-C., 1994, Mol. Cell. Biol., 14, 3242
- Bae S. C., 1993, Oncogene, 8, 809
- Gottschalk L. R., 1990, Mol. Cell. Biol., 10, 5486
- Redondo J. M., 1991, Mol. Cell. Biol., 11, 5671
- Wang S., 1993, EMBO J., 13, 3324
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Okuda, T., van Deursen, J., Hiebert, S. W., Grosveld, G., & Downing, J. R. (1996) · Cell · doi:10.1016/s0092-8674(00)80986-1 · Cited by 1,856
Self-citationLöwenberg, B., Downing, J. R., & Burnett, A. K. (1999) · New England Journal of Medicine · doi:10.1056/nejm199909303411407 · Cited by 1,283
Song, W.-J., Sullivan, M. G., Legare, R. D., Hutchings, S., Tan, X., Kufrin, D., Ratajczak, J., Resende, I. C., Haworth, C., Hock, R., et al. (1999) · Nature Genetics · doi:10.1038/13793 · Cited by 1,100
Transcription Factors, Normal Myeloid Development, and Leukemia
Tenen, D. G., Hromas, R., Licht, J. D., & Zhang, D.-E. (1997) · Blood · doi:10.1182/blood.v90.2.489 · Cited by 735
Core-binding factors in haematopoiesis and leukaemia
Speck, N. A., & Gilliland, D. G. (2002) · Nature reviews. Cancer · doi:10.1038/nrc840 · Cited by 575
Metadata
- Authors
- Meyers, S., Lenny, N., & Hiebert, S. W.
- Affiliations
- St. Jude Children's Research Hospital (US)
- ORCID
- Hiebert, Scott W.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1995-04-01
- Volume
- 15
- Issue
- 4
- Pages
- 1974-1982
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC230424
- OpenAlex
- W2113086962
- Cited by
- 368
- References
- 46
- Citations by year
- 2025: 1 · 2023: 3 · 2022: 2 · 2021: 1 · 2020: 2 · 2019: 3 · 2018: 4 · 2017: 3 · 2016: 5 · 2015: 8 · 2014: 4 · 2013: 11 · 2012: 3
- FWCI
- 18.34
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular and Cellular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- American Lebanese Syrian Associated Charities
- National Institutes of Health
- National Cancer Institute (R01CA064140, P30CA021765)
- Topics
- Health Sciences › Medicine › Hematology › Acute Myeloid Leukemia Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinoids in leukemia and cellular processes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- ETO, CBFβ, acute myeloid leukemia, transcriptional activation, alternative splicing, DNA-binding domain, nuclear localization, chromosomal translocation
- MeSH terms
- RUNX1 Translocation Partner 1 Protein, Amino Acid Sequence, Base Sequence, Cell Compartmentation, Cell Nucleus, Cloning, Molecular, DNA-Binding Proteins, Enhancer Elements, Genetic, Gene Expression Regulation, Molecular Sequence Data, Neoplasm Proteins, Protein Binding, Proto-Oncogene Proteins, Recombinant Fusion Proteins, Transcription Factors, Transcription, Genetic, Leukemia, Myeloid, Acute, Transcriptional Activation, Consensus Sequence, Alternative Splicing, DNA, Complementary, Core Binding Factors, Core Binding Factor Alpha 2 Subunit
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