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In Vivo and In Vitro Arginine Methylation of RNA-Binding Proteins
Open accessAbstract
Heterogenous nuclear ribonucleoproteins (hnRNPs) bind pre-mRNAs and facilitate their processing into mRNAs. Many of the hnRNPs undergo extensive posttranslational modifications including methylation on arginine residues. hnRNPs contain about 65% of the total NG,NG-dimethylarginine found in the cell nucleus. The role of this modification is not known. Here we identify the hnRNPs that are methylated in HeLa cells and demonstrate that most of the pre-mRNA-binding proteins receive this modification. Using recombinant human hnRNP A1 as a substrate, we have partially purified and characterized a protein-arginine N-methyltransferase specific for hnRNPs from HeLa cells. This methyltransferase can methylate the same subset of hnRNPs in vitro as are methylated in vivo. Furthermore, it can also methylate other RNA-binding proteins that contain the RGG motif RNA-binding domain. This activity is evolutionarily conserved from lower eukaryotes to mammals, suggesting that methylation has a significant role in the function of RNA-binding proteins.
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Cited by
Schug, K. A., & Lindner, W. (2005) · Chemical Reviews · doi:10.1021/cr040603j · Cited by 579
Role of Protein Methylation in Regulation of Transcription
Lee, D. Y., Teyssier, C., Strahl, B. D., & Stallcup, M. R. (2005) · Endocrine Reviews · doi:10.1210/er.2004-0008 · Cited by 455
Pawlak, M. R., Scherer, C. A., Chen, J., Roshon, M. J., & Ruley, H. E. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.13.4859-4869.2000 · Cited by 355
Yadav, N., Lee, J., Kim, J., Shen, J., Hu, M. C.-T., Aldaz, C. M., & Bedford, M. T. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1232272100 · Cited by 287
Protein arginine methyltransferases: promising targets for cancer therapy
Hwang, J. W., Cho, Y., Bae, G.-U., Kim, S.-N., & Kim, Y. K. (2021) · Experimental & Molecular Medicine · doi:10.1038/s12276-021-00613-y · Cited by 268
Metadata
- Authors
- Liu, Q., & Dreyfuss, G.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1995-05-01
- Volume
- 15
- Issue
- 5
- Pages
- 2800-2808
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2151960388
- Cited by
- 305
- References
- 0
- FWCI
- 3.20
- Type
- journal article
- Language
- en
- Open access
- green
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- Liu, Q., & Dreyfuss, G. (1995). In Vivo and In Vitro Arginine Methylation of RNA-Binding Proteins. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.15.5.2800
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