The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Identification of Target Genes for the Ewing's Sarcoma EWS/FLI Fusion Protein by Representational Difference Analysis
Open accessAbstract
The EWS/FLI-1 fusion gene results from the 11;22 chromosomal translocation in Ewing's sarcoma. The product of the gene is one of a growing number of structurally altered transcription factors implicated in oncogenesis. We have employed a subtractive cloning strategy of representational difference analysis in conjunction with a model transformation system to identify genes transcribed in response to EWS/FLI. We have characterized eight transcripts that are dependent on EWS/FLI for expression and two transcripts that are repressed in response to EWS/FLI. Three of the former were identified by sequence analysis as stromelysin 1, a murine homolog of cytochrome P-450 F1 and cytokeratin 15. Stromelysin 1 is induced rapidly after expression of EWS/FLI, suggesting that the stromelysin 1 gene may be a direct target gene of EWS/FLI. These results demonstrate that expression of EWS/FLI leads to significant changes in the transcription of specific genes and that these effects are at least partially distinct from those caused by expression of germ line FLI-1. The representational difference analysis technique can potentially be applied to investigate transformation pathways activated by a broad array of genes in different tumor systems.
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Cites
Gene fusion with an ETS DNA-binding domain caused by chromosome translocation in human tumours
Delattre, O., Zucman, J., Plougastel, B., Desmaze, C., Melot, T., Peter, M., Kovar, H., Joubert, I., de Jong, P., Rouleau, G., et al. (1992) · Nature · doi:10.1038/359162a0 · Cited by 1,907
The FLI-1 and chimeric EWS-FLI-1 oncoproteins display similar DNA binding specificities.
Mao, X., Miesfeldt, S., Yang, H., Leiden, J. M., & Thompson, C. B. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)32437-7 · Cited by 132
Cited by
ETS transcription factors and their emerging roles in human cancer
Seth, A., & Watson, D. K. (2005) · European Journal of Cancer · doi:10.1016/j.ejca.2005.08.013 · Cited by 469
Molecular Biology of the Ewing’s Sarcoma/Primitive Neuroectodermal Tumor Family
de Alava, E., & Gerald, W. L. (2000) · Journal of Clinical Oncology · doi:10.1200/jco.2000.18.1.204 · Cited by 360
Expression profiling of EWS/FLI identifies NKX2.2 as a critical target gene in Ewing's sarcoma
Smith, R., Owen, L. A., Trem, D. J., Wong, J. S., Whangbo, J. S., Golub, T. R., & Lessnick, S. L. (2006) · Cancer Cell · doi:10.1016/j.ccr.2006.04.004 · Cited by 351
Self-citationTanaka, K., Iwakuma, T., Harimaya, K., Sato, H., & Iwamoto, Y. (1997) · Journal of Clinical Investigation · doi:10.1172/jci119152 · Cited by 277
EWS–ETS oncoproteins: The linchpins of Ewing tumors
Janknecht, R. (2005) · Gene · doi:10.1016/j.gene.2005.08.007 · Cited by 173
Metadata
- Authors
- Braun, B. S., Frieden, R., Lessnick, S. L., May, W. A., & Denny, C. T.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1995-08
- Volume
- 15
- Issue
- 8
- Pages
- 4623-4630
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2147598399
- Cited by
- 162
- References
- 2
- FWCI
- 12.49
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Braun, B. S., Frieden, R., Lessnick, S. L., May, W. A., & Denny, C. T. (1995). Identification of Target Genes for the Ewing's Sarcoma EWS/FLI Fusion Protein by Representational Difference Analysis. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.15.8.4623
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