The DOI matches its Crossref record; 64 of the 64 references it cites were found in a registry or an index.
Cytoplasmic Localization of Human cdc25C during Interphase Requires an Intact 14-3-3 Binding Site
Open accessAbstract
cdc25C induces mitosis by activating the cdc2-cyclin B complex. The intracellular localization of cyclin B1 is regulated in a cell cycle-specific manner, and its entry into the nucleus may be required for the initiation of mitosis. To determine the cellular localization of cdc25C, monoclonal antibodies specific for cdc25C were developed and used to demonstrate that in human cells, cdc25C is retained in the cytoplasm during interphase. A deletion analysis identified a 58-amino-acid region (amino acids 201 to 258) in cdc25C that was required for the cytoplasmic localization of cdc25C. This region contained a specific binding site for 14-3-3 proteins, and mutations in cdc25C that disrupted 14-3-3 binding also disrupted the cytoplasmic localization of cdc25C during interphase. cdc25C proteins that do not contain a binding site for 14-3-3 proteins showed a pancellular localization and an increased ability to induce premature chromosome condensation. The cytoplasmic localization of cdc25C was not altered by gamma irradiation or treatment with the nuclear export inhibitor leptomycin B. These results suggest that 14-3-3 proteins may negatively regulate cdc25C function by sequestering cdc25C in the cytoplasm.
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Cited by
The DNA damage response: putting checkpoints in perspective
Zhou, B.-B. S., & Elledge, S. J. (2000) · Nature · doi:10.1038/35044005 · Cited by 3,213
ATR-Mediated Checkpoint Pathways Regulate Phosphorylation and Activation of Human Chk1
Zhao, H., & Piwnica-Worms, H. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.13.4129-4139.2001 · Cited by 1,091
14-3-3 transits to the nucleus and participates in dynamic nucleocytoplasmic transport
Brunet, A., Kanai, F., Stehn, J., Xu, J., Sarbassova, D., Frangioni, J. V., Dalal, S. N., DeCaprio, J. A., Greenberg, M. E., & Yaffe, M. B. (2002) · The Journal of Cell Biology · doi:10.1083/jcb.200112059 · Cited by 561
Self-citationCHK2 kinase in the DNA damage response and beyond
Zannini, L., Delia, D., & Buscemi, G. (2014) · Journal of Molecular Cell Biology · doi:10.1093/jmcb/mju045 · Cited by 455
Regulating mammalian checkpoints through Cdc25 inactivation
Donzelli, M., & Draetta, G. F. (2003) · EMBO reports · doi:10.1038/sj.embor.embor887 · Cited by 438
Metadata
- Authors
- Dalal, S. N., Schweitzer, C. M., Gan, J., & DeCaprio, J. A.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1999-06
- Volume
- 19
- Issue
- 6
- Pages
- 4465-4479
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W1914194999
- Cited by
- 279
- References
- 64
- FWCI
- 7.94
- Type
- journal article
- Language
- en
- Open access
- green
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- Dalal, S. N., Schweitzer, C. M., Gan, J., & DeCaprio, J. A. (1999). Cytoplasmic Localization of Human cdc25C during Interphase Requires an Intact 14-3-3 Binding Site. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.19.6.4465
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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