The DOI matches its Crossref record.
High-efficiency cloning of full-length cDNA.
Abstract
A widely recognized difficulty of presently used methods for cDNA cloning is obtaining cDNA segments that contain the entire nucleotide sequence of the corresponding mRNA. The cloning procedure described here mitigates this shortcoming. Of the 10(5) plasmid-cDNA recombinants obtained per microgram of rabbit reticulocyte mRNA, about 10% contained a complete alpha- of beta-globin mRNA sequence, and at least 30 to 50%, but very likely more, contained the entire globin coding regions. We attribute the high efficiency of cloning full- or nearly full-length cDNA to (i) the fact that the plasmid DNA vector itself serves as the primer for first- and second-strand cDNA synthesis, (ii) the lack of any nuclease treatment of the products, and (iii) the fact that one of the steps in the procedure results in preferential cloning of recombinants with full-length cDNA's over those with truncated cDNA's.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
The human LDL receptor: A cysteine-rich protein with multiple Alu sequences in its mRNA
Yamamoto, T., Davis, C. G., Brown, M. S., Schneider, W. J., Casey, M. L., Goldstein, J. L., & Russell, D. W. (1984) · Cell · doi:10.1016/0092-8674(84)90188-0 · Cited by 1,382
Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor.
Samal, B., Sun, Y., Stearns, G., Xie, C., Suggs, S., & McNiece, I. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.2.1431 · Cited by 1,019
Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression
Okubo, K., Hori, N., Matoba, R., Niiyama, T., Fukushima, A., Kojima, Y., & Matsubara, K. (1992) · Nature Genetics · doi:10.1038/ng1192-173 · Cited by 522
Insulin-like growth factor-I is an essential regulator of the differentiation of 3T3-L1 adipocytes.
Smith, P. J., Wise, L. S., Berkowitz, R., Wan, C., & Rubin, C. S. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)76555-7 · Cited by 517
From tissues to cell types and back: single-cell gene expression analysis of tissue architecture
Chen, X., Teichmann, S. A., & Meyer, K. B. (2018) · Annual Review of Biomedical Data Science · doi:10.1146/annurev-biodatasci-080917-013452 · Cited by 112
Metadata
- Authors
- Okayama, H., & Berg, P.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1982-02
- Volume
- 2
- Issue
- 2
- Pages
- 161-170
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W4213231968
- Cited by
- 758
- References
- 0
- FWCI
- 19.31
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: High-efficiency cloning of full-length cDNA. https://app.cochat.ai/papers/10.1128/mcb.2.2.161
- The paper
- Okayama, H., & Berg, P. (1982). High-efficiency cloning of full-length cDNA. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.2.2.161
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
