The human LDL receptor: A cysteine-rich protein with multiple Alu sequences in its mRNA
Abstract
The nucleotide sequence of a cloned 5.3 kilobase cDNA for the human low density lipoprotein receptor revealed five domains in the 839 amino acid protein: 322 NH2-terminal amino acids, extremely rich in disulfide-bonded cysteine residues (15%) and including an 8-fold repeat of 40 residues that may contain the LDL binding site; 350 residues homologous to the precursor of mouse epidermal growth factor; a region immediately outside the plasma membrane, rich in serine and threonine and the site of O-linked glycosylation; 22 hydrophobic amino acids, spanning the plasma membrane; and 50 COOH-terminal amino acids, projecting into the cytoplasm. The mRNA for the receptor contains a 3' untranslated region of 2.5 kilobases that includes multiple copies of the Alu family of repetitive DNAs. Transfection of simian COS cells with the human LDL receptor cDNA linked to the SV40 early promoter resulted in expression of functional cell surface receptors.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Positively charged amino acids in the receptor-binding region form ionic bonds with negatively charged residues in a consensus sequence repeated seven times in the ligand-binding region of the apo B,E (LDL) receptor.
“The positively charged amino acids in this region form ionic bonds with negatively charged residues in the consensus se- quence, which is repeated seven times in the ligand-binding region ofthe apo B,E (LDL) receptor (23).”
From Apolipoprotein E2-Christchurch (136 Arg----Ser). New variant of human apolipoprotein E in a patient with type III hyperlipoproteinemia. (1987) · cites this paper as [23]
Cited by
Sorted by citations
A Receptor-Mediated Pathway for Cholesterol Homeostasis
Brown, M. S., & Goldstein, J. L. (1986) · Science · doi:10.1126/science.3513311 · Cited by 5,703 · see citing works
Self-citationApolipoprotein E: Cholesterol Transport Protein with Expanding Role in Cell Biology
Mahley, R. W. (1988) · Science · doi:10.1126/science.3283935 · Cited by 4,028 · see citing works
Kong, W., Wei, J., Abidi, P., Lin, M., Inaba, S., Li, C., Wang, Y., Wang, Z., Si, S., Pan, H., et al. (2004) · Nature Medicine · doi:10.1038/nm1135 · Cited by 1,391 · see citing works
A Century of Cholesterol and Coronaries: From Plaques to Genes to Statins
Goldstein, J. L., & Brown, M. S. (2015) · Cell · doi:10.1016/j.cell.2015.01.036 · Cited by 1,233 · see citing works
Self-citationGene transfer and molecular cloning of the rat nerve growth factor receptor
Radeke, M. J., Misko, T. P., Hsu, C., Herzenberg, L. A., & Shooter, E. M. (1987) · Nature · doi:10.1038/325593a0 · Cited by 938 · see citing works
Apolipoprotein E: Structure-Function Relationships
Weisgraber, K. H. (1994) · Advances in Protein Chemistry · doi:10.1016/s0065-3233(08)60642-7 · Cited by 830 · see citing works
Russell, D. W., & Setchell, K. D. R. (1992) · Biochemistry · doi:10.1021/bi00135a001 · Cited by 819 · see citing works
Structure of human steroid 21-hydroxylase genes.
White, P. C., New, M. I., & Dupont, B. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.14.5111 · Cited by 569 · see citing works
Relaxin family peptides and their receptors
Bathgate, R. A. D., Halls, M. L., van der Westhuizen, E. T., Callander, G. E., Kocan, M., & Summers, R. J. (2013) · Physiological Reviews · doi:10.1152/physrev.00001.2012 · Cited by 534 · see citing works
Collawn, J. F., Stangel, M., Kuhn, L. A., Esekogwu, V., Jing, S., Trowbridge, I. S., & Tainer, J. A. (1990) · Cell · doi:10.1016/0092-8674(90)90509-d · Cited by 518 · see citing works
[1] Introduction to the plasma lipoproteins
Gotto, A. M., Pownall, H. J., & Havel, R. J. (1986) · Methods in Enzymology · doi:10.1016/0076-6879(86)28061-1 · Cited by 421 · see citing works
Cloning of cDNA encoding steroid 11 beta-hydroxylase (P450c11).
Chua, S. C., Szabo, P., Vitek, A., Grzeschik, K. H., John, M., & White, P. C. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.20.7193 · Cited by 206 · see citing works
More than cholesterol transporters: lipoprotein receptors in CNS function and neurodegeneration
Lane-Donovan, C., Philips, G. T., & Herz, J. (2014) · Neuron · doi:10.1016/j.neuron.2014.08.005 · Cited by 158 · see citing works
Wardell, M. R., Brennan, S. O., Janus, E. D., Fraser, R., & Carrell, R. W. (1987) · Journal of Clinical Investigation · doi:10.1172/jci113096 · Cited by 159 · see citing works
Morel, Y., Bristow, J., Gitelman, S. E., & Miller, W. L. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.17.6582 · Cited by 158 · see citing works
Differential glycosylation regulates processing of lipoprotein receptors by γ-secretase
May, P., Bock, H. H., Nimpf, J., & Herz, J. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m305858200 · Cited by 142 · see citing works
Hofmann, S. L., Russell, D. W., Brown, M. S., Goldstein, J. L., & Hammer, R. E. (1988) · Science · doi:10.1126/science.3344433 · Cited by 117 · see citing works
Self-citationMechanism and consequences of the duplication of the human C4/P450c21/gene X locus
Gitelman, S. E., Bristow, J., & Miller, W. L. (1992) · Molecular and Cellular Biology · doi:10.1128/mcb.12.5.2124 · Cited by 106 · see citing works
Abifadel, M., Rabès, J.-P., Jambart, S., Halaby, G., Gannagé-Yared, M.-H., Sarkis, A., Beaino, G., Varret, M., Salem, N., Corbani, S., et al. (2009) · Human Mutation · doi:10.1002/humu.21002 · Cited by 99 · see citing works
Chung, B. C., Matteson, K. J., & Miller, W. L. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.12.4243 · Cited by 95 · see citing works
Patalay, M., Lofgren, I. E., Freake, H. C., Koo, S. I., & Fernandez, M. L. (2005) · The Journal of Nutrition · doi:10.1093/jn/135.4.735 · Cited by 53 · see citing works
Ranganathan, S., Noyes, N. C., Migliorini, M., Winkles, J. A., Battey, F. D., Hyman, B. T., Smith, E., Yepes, M., Mikhailenko, I., & Strickland, D. K. (2011) · Journal of Neuroscience · doi:10.1523/jneurosci.5065-10.2011 · Cited by 49 · see citing works
“…domain containing an NPXY internalization sequence, a transmembrane domain, an O-linked sugar domain, epidermal growth factor-like repeats, a -propeller domain, and a cluster of cysteine-rich LDL receptor type A (LDLa) repeats that are responsible for binding the ligands (Yamamoto et al., 1984).”
BackgroundJones, R. J., Gu, D., Bjorklund, C. C., Kuiatse, I., Remaley, A. T., Bashir, T., Vreys, V., & Orlowski, R. Z. (2013) · The Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.113.204958 · Cited by 26 · see citing works
- Authors
- Yamamoto, T., Davis, C. G., Brown, M. S., Schneider, W. J., Casey, M. L., Goldstein, J. L., & Russell, D. W.
- Affiliations
- University of Texas Health Science Center at Dallas (US)
- The University of Texas at San Antonio Health Science Center (US)
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1984-11
- Volume
- 39
- Issue
- 1
- Pages
- 27-38
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W1995032745
- Cited by
- 1,382
- References
- 0
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 20 · 2023: 9 · 2022: 2 · 2021: 14 · 2020: 12 · 2019: 7 · 2018: 12 · 2017: 7 · 2016: 13 · 2015: 22 · 2014: 19 · 2013: 18 · 2012: 12
- FWCI
- 133.04
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute for Health and Care Research
- National Institutes of Health
- Topics
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Keywords
- LDL receptor, cysteine-rich proteins, disulfide bonds, 3' untranslated region, O-glycosylation, cDNA cloning, SV40 promoter
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Cell Line, Chlorocebus aethiops, Cloning, Molecular, Cysteine, DNA, DNA Restriction Enzymes, Humans, Kidney, Lipoproteins, LDL, Plasmids, Receptors, LDL, RNA, Messenger, Transfection
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
