The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Protein 4.1 R-135 Interacts with a Novel Centrosomal Protein (CPAP) Which Is Associated with the γ-Tubulin Complex
Open accessAbstract
Using a yeast two-hybrid system, we isolated a novel human centrosomal protein, CPAP (centrosomal P4.1-associated protein), which specifically interacts with the head domain of the 135-kDa protein 4.1R isoform (4.1R-135). Sequence analysis revealed that the carboxyl terminus of CPAP has 31.3% amino acid identity with human Tcp-10 (a t-complex responder gene product). Interestingly, most of the sequence identity is restricted to two conserved regions. One carries a leucine zipper, which may form a series of heptad repeats involved in coiled-coil formation; the other contains unusual glycine repeats with unknown function. Immunofluorescence analysis revealed that CPAP and gamma-tubulin are localized within the centrosome throughout the cell cycle. CPAP cosediments with gamma-tubulin in sucrose gradients and coimmunoprecipitates with gamma-tubulin, indicating that CPAP is a part of the gamma-tubulin complex. Furthermore, functional analysis revealed that CPAP is localized within the center of microtubule asters and may participate in microtubule nucleation. The formation of microtubule asters was significantly inhibited by anti-CPAP antibody. Together, these observations indicate that CPAP may play an important role in cell division and centrosome function.
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Cites
Predicting coiled coils from protein sequences.
Lupas, A., Van Dyke, M., & Stock, J. (1991) · Science · doi:10.1126/science.252.5009.1162 · Cited by 3,953
Cited by
Woods, C. G., Bond, J., & Enard, W. (2005) · The American Journal of Human Genetics · doi:10.1086/429930 · Cited by 431
SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells
Leidel, S., Delattre, M., Cerutti, L., Baumer, K., & Gönczy, P. (2005) · Nature Cell Biology · doi:10.1038/ncb1220 · Cited by 377
Control of Centriole Length by CPAP and CP110
Schmidt, T. I., Kleylein-Sohn, J., Westendorf, J., Le Clech, M., Lavoie, S. B., Stierhof, Y.-D., & Nigg, E. A. (2009) · Current Biology · doi:10.1016/j.cub.2009.05.016 · Cited by 345
CPAP is a cell-cycle regulated protein that controls centriole length
Tang, C.-J. C., Fu, R.-H., Wu, K.-S., Hsu, W.-B., & Tang, T. K. (2009) · Nature Cell Biology · doi:10.1038/ncb1889 · Cited by 289
Self-citationCENP-32 is required to maintain centrosomal dominance in bipolar spindle assembly
Ohta, S., Wood, L., Toramoto, I., Yagyu, K.-I., Fukagawa, T., & Earnshaw, W. C. (2015) · Molecular Biology of the Cell · doi:10.1091/mbc.e14-09-1366 · Cited by 16
Metadata
- Authors
- Hung, L.-Y., Tang, C.-J. C., & Tang, T. K.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2000-10-15
- Volume
- 20
- Issue
- 20
- Pages
- 7813-7825
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2156673839
- Cited by
- 167
- References
- 1
- FWCI
- 1.85
- Type
- journal article
- Language
- en
- Open access
- green
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- Hung, L.-Y., Tang, C.-J. C., & Tang, T. K. (2000). Protein 4.1 R-135 Interacts with a Novel Centrosomal Protein (CPAP) Which Is Associated with the γ-Tubulin Complex. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.20.20.7813-7825.2000
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