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Cited by 16Microtubule and mitosis dynamicsGenomics and Chromatin DynamicsNuclear Structure and Function

CENP-32 is required to maintain centrosomal dominance in bipolar spindle assembly

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Ohta, Shinya, Wood, Laura, Toramoto, Iyo, Yagyu, Ken-Ichi, Fukagawa, Tatsuo, Earnshaw, William C.·2015·Molecular Biology of the Cell
DOI 10.1091/mbc.e14-09-1366PMC PMC4454171
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Abstract

Centrosomes nucleate spindle formation, direct spindle pole positioning, and are important for proper chromosome segregation during mitosis in most animal cells. We previously reported that centromere protein 32 (CENP-32) is required for centrosome association with spindle poles during metaphase. In this study, we show that CENP-32 depletion seems to release centrosomes from bipolar spindles whose assembly they had previously initiated. Remarkably, the resulting anastral spindles function normally, aligning the chromosomes to a metaphase plate and entering anaphase without detectable interference from the free centrosomes, which appear to behave as free asters in these cells. The free asters, which contain reduced but significant levels of CDK5RAP2, show weak interactions with spindle microtubules but do not seem to make productive attachments to kinetochores. Thus CENP-32 appears to be required for centrosomes to integrate into a fully functional spindle that not only nucleates astral microtubules, but also is able to nucleate and bind to kinetochore and central spindle microtubules. Additional data suggest that NuMA tethers microtubules at the anastral spindle poles and that augmin is required for centrosome detachment after CENP-32 depletion, possibly due to an imbalance of forces within the spindle.

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Authors
Ohta, S., Wood, L., Toramoto, I., Yagyu, K.-I., Fukagawa, T., & Earnshaw, W. C.
Affiliations
  • University of Kochi (JP)
  • Wellcome Centre for Cell Biology (GB)
  • Medical School, Kochi University, Kohasu, Oko-cho, Nankoku, Kochi 783-8505, Japan
  • National Institute of Genetics (JP)
Corresponding author
  • Ohta, Shinya
  • Earnshaw, William C.
ORCID
Venue
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Published
2015-04
Volume
26
Issue
7
Pages
1225-1237
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
DOI
10.1091/mbc.e14-09-1366
PMC
PMC4454171
OpenAlex
W2147955440
Cited by
16
References
2
Citations by year
2026: 5 · 2025: 1 · 2024: 2 · 2023: 2 · 2022: 2 · 2020: 1 · 2018: 1 · 2016: 1 · 2015: 1
FWCI
0.48
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • Molecular Biology of the Cell (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • Edinburgh Research Explorer (University of Edinburgh) (submittedVersion, cc-by-nc-sa)
Funders
  • Wellcome Trust (077707, 092076, 073915, 107022/Z/15/Z)
  • Japan Society for the Promotion of Science (25870487)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
centrosome, bipolar spindle assembly, CDK5RAP2, kinetochore attachment, NUMA, augmin, astral microtubules, mitosis, chromosome segregation
MeSH terms
Chromosomal Proteins, Non-Histone, Humans, Microtubules, Spindle Apparatus, Centrosome, Kinetochores, Cell Cycle Proteins, Nuclear Matrix-Associated Proteins, Antigens, Nuclear

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