The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Acetylation-Dependent Chromatin Reorganization by BRDT, a Testis-Specific Bromodomain-Containing Protein
Open accessAbstract
The association between histone acetylation and replacement observed during spermatogenesis prompted us to consider the testis as a source for potential factors capable of remodelling acetylated chromatin. A systematic search of data banks for open reading frames encoding testis-specific bromodomain-containing proteins focused our attention on BRDT, a testis-specific protein of unknown function containing two bromodomains. BRDT specifically binds hyperacetylated histone H4 tail depending on the integrity of both bromodomains. Moreover, in somatic cells, the ectopic expression of BRDT triggered a dramatic reorganization of the chromatin only after induction of histone hyperacetylation by trichostatin A (TSA). We then defined critical domains of BRDT involved in its activity. Both bromodomains of BRDT, as well as flanking regions, were found indispensable for its histone acetylation-dependent remodelling activity. Interestingly, we also observed that recombinant BRDT was capable of inducing reorganization of the chromatin of isolated nuclei in vitro only when the nuclei were from TSA-treated cells. This assay also allowed us to show that the action of BRDT was ATP independent, suggesting a structural role for the protein in the remodelling of acetylated chromatin. This is the first demonstration of a large-scale reorganization of acetylated chromatin induced by a specific factor.
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Cites
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Cited by
Tan, M., Luo, H., Lee, S., Jin, F., Yang, J. S., Montellier, E., Buchou, T., Cheng, Z., Rousseaux, S., Rajagopal, N., et al. (2011) · Cell · doi:10.1016/j.cell.2011.08.008 · Cited by 1,902
Self-citationProtamines and male infertility
Oliva, R. (2006) · Human Reproduction Update · doi:10.1093/humupd/dml009 · Cited by 802
Cooperative binding of two acetylation marks on a histone tail by a single bromodomain
Morinière, J., Rousseaux, S., Steuerwald, U., Soler-López, M., Curtet, S., Vitte, A.-L., Govin, J., Gaucher, J., Sadoul, K., Hart, D. J., et al. (2009) · Nature · doi:10.1038/nature08397 · Cited by 469
Self-citationHistone demethylase JHDM2A is critical for Tnp1 and Prm1 transcription and spermatogenesis
Okada, Y., Scott, G., Ray, M. K., Mishina, Y., & Zhang, Y. (2007) · Nature · doi:10.1038/nature06236 · Cited by 388
Altered protamine expression and diminished spermatogenesis: what is the link?
Carrell, D. T., Emery, B. R., & Hammoud, S. (2007) · Human Reproduction Update · doi:10.1093/humupd/dml057 · Cited by 386
Metadata
- Authors
- Pivot-Pajot, C., Caron, C., Govin, J., Vion, A., Rousseaux, S., & Khochbin, S.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2003-08-01
- Volume
- 23
- Issue
- 15
- Pages
- 5354-5365
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2107911443
- Cited by
- 285
- References
- 38
- FWCI
- 3.11
- Type
- journal article
- Language
- en
- Open access
- green
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- Pivot-Pajot, C., Caron, C., Govin, J., Vion, A., Rousseaux, S., & Khochbin, S. (2003). Acetylation-Dependent Chromatin Reorganization by BRDT, a Testis-Specific Bromodomain-Containing Protein. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.23.15.5354-5365.2003
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