The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Intracellular Reactive Oxygen Species Activate Src Tyrosine Kinase during Cell Adhesion and Anchorage-Dependent Cell Growth
Open accessAbstract
Src tyrosine kinases are central components of adhesive responses and are required for cell spreading onto the extracellular matrix. Among other intracellular messengers elicited by integrin ligation are reactive oxygen species, which act as synergistic mediators of cytoskeleton rearrangement and cell spreading. We report that after integrin ligation, the tyrosine kinase Src is oxidized and activated. Src displays an early activation phase, concurrent with focal adhesion formation and driven mainly by Tyr527 dephosphorylation, and a late phase, concomitant with reactive oxygen species production, cell spreading, and integrin-elicited kinase oxidation. In addition, our results suggest that reactive oxygen species are key mediators of in vitro and in vivo v-Src tumorigenic properties, as both antioxidant treatments and the oxidant-insensitive C245A and C487A Src mutants greatly decrease invasivity, serum-independent and anchorage-independent growth, and tumor onset. Therefore we propose that, in addition to the known phosphorylation/dephosphorylation circuitry, redox regulation of Src activity is required during both cell attachment to the extracellular matrix and tumorigenesis.
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Cites
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Cited by
Regulation of cancer cell metabolism
Cairns, R. A., Harris, I. S., & Mak, T. W. (2011) · Nature Reviews Cancer · doi:10.1038/nrc2981 · Cited by 4,744
H 2 O 2 , a Necessary Evil for Cell Signaling
Rhee, S. G. (2006) · Science · doi:10.1126/science.1130481 · Cited by 2,160
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Radical-free biology of oxidative stress
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The Two Faces of Reactive Oxygen Species in Cancer
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“F or p er so na l u se o nl y. cells (Giannoni et al. 2005).”
Background
Metadata
- Authors
- Giannoni, E., Buricchi, F., Raugei, G., Ramponi, G., & Chiarugi, P.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2005-08-01
- Volume
- 25
- Issue
- 15
- Pages
- 6391-6403
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2166499489
- Cited by
- 455
- References
- 36
- FWCI
- 5.19
- Type
- journal article
- Language
- en
- Open access
- green
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- Giannoni, E., Buricchi, F., Raugei, G., Ramponi, G., & Chiarugi, P. (2005). Intracellular Reactive Oxygen Species Activate Src Tyrosine Kinase during Cell Adhesion and Anchorage-Dependent Cell Growth. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.25.15.6391-6403.2005
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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