The DOI matches its Crossref record; 59 of the 59 references it cites were found in a registry or an index.
High-Resolution Genome-Wide Mapping of Transposon Integration in Mammals
Open accessAbstract
The Sleeping Beauty (SB) transposon is an emerging tool for transgenesis, gene discovery, and therapeutic gene delivery in mammals. Here we studied 1,336 SB insertions in primary and cultured mammalian cells in order to better understand its target site preferences. We report that, although widely distributed, SB integration recurrently targets certain genomic regions and shows a small but significant bias toward genes and their upstream regulatory sequences. Compared to those of most integrating viruses, however, the regional preferences associated with SB-mediated integration were much less pronounced and were not significantly influenced by transcriptional activity. Insertions were also distinctly nonrandom with respect to intergenic sequences, including a strong bias toward microsatellite repeats, which are predominantly enriched in noncoding DNA. Although we detected a consensus sequence consistent with a twofold dyad symmetry at the target site, the most widely used sites did not match this consensus. In conjunction with an observed SB integration preference for bent DNA, these results suggest that physical properties may be the major determining factor in SB target site selection. These findings provide basic insights into the transposition process and reveal important distinctions between transposon- and virus-based integrating vectors.
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LMO2 -Associated Clonal T Cell Proliferation in Two Patients after Gene Therapy for SCID-X1
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Transcription Start Regions in the Human Genome Are Favored Targets for MLV Integration
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Self-citationRetroviral DNA Integration: ASLV, HIV, and MLV Show Distinct Target Site Preferences
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Martinez, M., & Moon, E. K. (2019) · Frontiers in Immunology · doi:10.3389/fimmu.2019.00128 · Cited by 851
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Sun, J., Ramos, A., Chapman, B., Johnnidis, J. B., Le, L., Ho, Y.-J., Klein, A., Hofmann, O., & Camargo, F. D. (2014) · Nature · doi:10.1038/nature13824 · Cited by 821
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Genome-wide analysis of retroviral DNA integration
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Metadata
- Authors
- Yant, S. R., Wu, X., Huang, Y., Garrison, B., Burgess, S. M., & Kay, M. A.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2005-03-01
- Volume
- 25
- Issue
- 6
- Pages
- 2085-2094
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W1970491071
- Cited by
- 318
- References
- 59
- FWCI
- 12.44
- Type
- journal article
- Language
- en
- Open access
- green
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- Yant, S. R., Wu, X., Huang, Y., Garrison, B., Burgess, S. M., & Kay, M. A. (2005). High-Resolution Genome-Wide Mapping of Transposon Integration in Mammals. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.25.6.2085-2094.2005
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