The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Production of human beta interferon in insect cells infected with a baculovirus expression vector.
Open accessAbstract
Autographa californica nuclear polyhedrosis virus (AcNPV) was used as an expression vector for human beta interferon. By using specially constructed plasmids, the protein-coding sequences for interferon were linked to the AcNPV promoter for the gene encoding for polyhedrin, the major occlusion protein. The interferon gene was inserted at various locations relative to the AcNPV polyhedrin transcriptional and translational signals, and the interferon-polyhedrin hybrid genes were transferred to infectious AcNPV expression vectors. Biologically active interferon was produced, and greater than 95% was secreted from infected insect cells. A maximum of ca. 5 X 10(6) U of interferon activity was produced by 10(6) infected cells. These results demonstrate that AcNPV should be suitable for use as a eucaryotic expression vector for the production of products from cloned genes.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Cited by
Kozak, M. (1986) · Cell · doi:10.1016/0092-8674(86)90762-2 · Cited by 4,741
Protein production and purification
Structural Genomics Consortium, Biologiques, A. E. F. D. M., Berkeley Structural Genomics Center, China Structural Genomics Consortium, Integrated Center for Structure and Function Innovation, Israel Structural Proteomics Center, Joint Center for Structural Genomics, Midwest Center for Structural Genomics, New York Structural GenomiX Research Center for Structural Genomics, Northeast Structural Genomics Consortium, et al. (2008) · Nature Methods · doi:10.1038/nmeth.f.202 · Cited by 916
Clinical evidence for the safety of GAD65 immunomodulation in adult-onset autoimmune diabetes
Agardh, C.-D., Cilio, C. M., Lethagen, Å., Lynch, K., Leslie, R. D. G., Palmér, M., Harris, R. A., Robertson, J. A., & Lernmark, Å. (2005) · Journal of Diabetes and its Complications · doi:10.1016/j.jdiacomp.2004.12.003 · Cited by 226
New baculovirus expression tools for recombinant protein complex production
Trowitzsch, S., Bieniossek, C., Nie, Y., Garzoni, F., & Berger, I. (2010) · Journal of Structural Biology · doi:10.1016/j.jsb.2010.02.010 · Cited by 224
Implementation of BacMam virus gene delivery technology in a drug discovery setting
Kost, T. A., Condreay, J. P., Ames, R. S., Rees, S., & Romanos, M. A. (2007) · Drug Discovery Today · doi:10.1016/j.drudis.2007.02.017 · Cited by 81
Metadata
- Authors
- Smith, G. E., Summers, M. D., & Fraser, M. J.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1983-12
- Volume
- 3
- Issue
- 12
- Pages
- 2156-2165
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W2170293208
- Cited by
- 968
- References
- 1
- FWCI
- 4.91
- Type
- journal article
- Language
- en
- Open access
- green
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- Smith, G. E., Summers, M. D., & Fraser, M. J. (1983). Production of human beta interferon in insect cells infected with a baculovirus expression vector. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.3.12.2156
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