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Pichia pastoris as a host system for transformations.
Open accessAbstract
We developed a methylotrophic yeast, Pichia pastoris, as a host for DNA transformations. The system is based on an auxotrophic mutant host of P. pastoris which is defective in histidinol dehydrogenase. As a selectable marker, we isolated and characterized the P. pastoris HIS4 gene. Plasmid vectors which contained either the P. pastoris or the Saccharomyces cerevisiae HIS4 gene transformed the P. pastoris mutant host. DNA transfer was accomplished by a modified version of the spheroplast generation (CaCl2-polyethylene glycol)-fusion procedure developed for S. cerevisiae. In addition, we report the isolation and characterization of P. pastoris DNA fragments with autonomous replication sequence activity. Two fragments, PARS1 and PARS2, when present on plasmids increased transformation frequencies to 10(5)/micrograms and maintained the plasmids as autonomous elements in P. pastoris cells.
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Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Cited by
Ahmad, M., Hirz, M., Pichler, H., & Schwab, H. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-5732-5 · Cited by 989
Daly, R., & Hearn, M. T. W. (2005) · Journal of Molecular Recognition · doi:10.1002/jmr.687 · Cited by 746
A hookworm glycoprotein that inhibits neutrophil function is a ligand of the integrin CD11b/CD18
Moyle, M., Foster, D. L., McGrath, D. E., Brown, S. M., Laroche, Y., De Meutter, J., Stanssens, P., Bogowitz, C. A., Fried, V. A., & Ely, J. A. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)36982-x · Cited by 253
Divergent modes of autophagy in the methylotrophic yeast Pichia pastoris
Tuttle, D. L., & Dunn, W. A. (1995) · Journal of Cell Science · doi:10.1242/jcs.108.1.25 · Cited by 206
Guan, J., Stromhaug, P. E., George, M. D., Habibzadegah-Tari, P., Bevan, A., Dunn, W. A., & Klionsky, D. J. (2001) · Molecular Biology of the Cell · doi:10.1091/mbc.12.12.3821 · Cited by 204
Metadata
- Authors
- Cregg, J. M., Barringer, K. J., Hessler, A. Y., & Madden, K. R.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1985-12
- Volume
- 5
- Issue
- 12
- Pages
- 3376-3385
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W1938341712
- Cited by
- 577
- References
- 1
- FWCI
- 3.36
- Type
- journal article
- Language
- en
- Open access
- green
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- Cregg, J. M., Barringer, K. J., Hessler, A. Y., & Madden, K. R. (1985). Pichia pastoris as a host system for transformations. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.5.12.3376
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
