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Cited by 989

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Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

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Ahmad, Mudassar, Hirz, Melanie, Pichler, Harald, Schwab, Helmut·2014·Applied Microbiology and Biotechnology
DOI 10.1007/s00253-014-5732-5
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Abstract

Pichia pastoris is an established protein expression host mainly applied for the production of biopharmaceuticals and industrial enzymes. This methylotrophic yeast is a distinguished production system for its growth to very high cell densities, for the available strong and tightly regulated promoters, and for the options to produce gram amounts of recombinant protein per litre of culture both intracellularly and in secretory fashion. However, not every protein of interest is produced in or secreted by P. pastoris to such high titres. Frequently, protein yields are clearly lower, particularly if complex proteins are expressed that are hetero-oligomers, membrane-attached or prone to proteolytic degradation. The last few years have been particularly fruitful because of numerous activities in improving the expression of such complex proteins with a focus on either protein engineering or on engineering the protein expression host P. pastoris. This review refers to established tools in protein expression in P. pastoris and highlights novel developments in the areas of expression vector design, host strain engineering and screening for high-level expression strains. Breakthroughs in membrane protein expression are discussed alongside numerous commercial applications of P. pastoris derived proteins.

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Metadata

Authors
Ahmad, M., Hirz, M., Pichler, H., & Schwab, H.
Venue
Applied Microbiology and Biotechnology
Publisher
Springer Science and Business Media LLC
Published
2014-04-18
Volume
98
Issue
12
Pages
5301-5317
ISSN
  • 0175-7598 (ISSN-L)
  • 1432-0614
DOI
10.1007/s00253-014-5732-5
OpenAlex
W2046437093
Cited by
989
References
100
FWCI
23.48
Type
journal article
Language
en
Open access
hybrid
Licence
creativecommons.org/licenses/by/4.0 (unspecified)

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Ahmad, M., Hirz, M., Pichler, H., & Schwab, H. (2014). Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-014-5732-5

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