Cited by 482Fungal and yeast genetics researchCRISPR and Genetic EngineeringDNA Repair Mechanisms

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Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae

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Sauer, B·1987·Molecular and Cellular Biology

DOI 10.1128/mcb.7.6.2087PMC PMC365329

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Abstract

The procaryotic cre-lox site-specific recombination system of coliphage P1 was shown to function in an efficient manner in a eucaryote, the yeast Saccharomyces cerevisiae. The cre gene, which codes for a site-specific recombinase, was placed under control of the yeast GALI promoter. lox sites flanking the LEU2 gene were integrated into two different chromosomes in both orientations. Excisive recombination at the lox sites (as measured by loss of the LEU2 gene) was promoted efficiently and accurately by the Cre protein and was dependent upon induction by galactose. These results demonstrate that a procaryotic recombinase can enter a eucaryotic nucleus and, moreover, that the ability of the Cre recombinase to perform precise recombination events on the chromosomes of S. cerevisiae is unimpaired by chromatin structure.

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Authors: Sauer, B.
Affiliations: DuPont (United States) (US)
Corresponding author: Sauer, B
Venue: Molecular and Cellular Biology
Publisher: Informa UK Limited
Published: 1987-06
Volume: 7
Issue: 6
Pages: 2087-2096
ISSN: 0270-7306 (ISSN-L)
1098-5549
DOI: 10.1128/mcb.7.6.2087
PMC: PMC365329
OpenAlex: W1913334531
Cited by: 482
References: 38
Citations by year: 2025: 11 · 2024: 9 · 2023: 15 · 2022: 17 · 2021: 17 · 2020: 15 · 2019: 20 · 2018: 27 · 2017: 15 · 2016: 19 · 2015: 21 · 2014: 34 · 2013: 18 · 2012: 21
FWCI: 1.79
Type: journal article
Language: en
Open access: green
europepmc.org
Locations: Molecular and Cellular Biology (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
Keywords: Cre recombinase, Saccharomyces cerevisiae, bacteriophage P1, excision, LEU2 gene, GAL1 promoter, galactose induction, chromatin structure, chromosomal integration
MeSH terms: Coliphages, DNA Nucleotidyltransferases, DNA Restriction Enzymes, Escherichia coli, Genes, Genes, Fungal, Genes, Viral, Plasmids, Promoter Regions, Genetic, Recombination, Genetic, Saccharomyces cerevisiae, Viral Proteins, Integrases

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Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae

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