Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961

Molecular Cloning: A Laboratory Manual

Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589

DNA sequencing with chain-terminating inhibitors

Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572

Detection of specific sequences among DNA fragments separated by gel electrophoresis

Southern, E. M. (1975) · Journal of Molecular Biology · doi:10.1016/s0022-2836(75)80083-0 · Cited by 32,245

Molecular Cloning. A Laboratory Manual

Wood, E. (1983) · Biochemical Education · doi:10.1016/0307-4412(83)90068-7 · Cited by 29,534

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I

Rigby, P. W. J., Dieckmann, M., Rhodes, C., & Berg, P. R. (1977) · Journal of Molecular Biology · doi:10.1016/0022-2836(77)90052-3 · Cited by 11,400

Studies on transformation of Escherichia coli with plasmids

Hanahan, D. (1983) · Journal of Molecular Biology · doi:10.1016/s0022-2836(83)80284-8 · Cited by 11,194

Methods in yeast genetics

Sherman, F., Fink, G. R., & Hicks, J. B. (1979) · Cold Spring Harbor Laboratory eBooks · Cited by 7,430

Methods in Yeast Genetics

Jacob, H. (1988) · Bioelectrochemistry and Bioenergetics · doi:10.1016/0302-4598(88)80052-7 · Cited by 4,911

DNA Topoisomerase II Is a Determinant of the Tensile Properties of Yeast Centromeric Chromatin and the Tension Checkpoint

Warsi, T. H., Navarro, M. S., & Bachant, J. (2008) · Molecular Biology of the Cell · doi:10.1091/mbc.e08-05-0547 · Cited by 32

Protein Sorting in the Late Golgi of Saccharomyces cerevisiae Does Not Require Mannosylated Sphingolipids

Lisman, Q., Pomorski, T. G., Vogelzangs, C., Urli, D. C., van Delwijnen, W. D. C., & Holthuis, J. C. M. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m306119200 · Cited by 32

Genetic characterization and construction of an auxotrophic strain ofSaccharomyces cerevisiaeJP1, a Brazilian industrial yeast strain for bioethanol production

Reis, V. C. B., Nicola, A. M., Neto, O. D. S. O., Batista, V. D. F., de Moraes, L. M. P., & Torres, F. A. G. (2012) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-012-1170-5 · Cited by 31

Study of Muscle Cell Dedifferentiation after Skeletal Muscle Injury of Mice with a Cre-Lox System

Mu, X., Peng, H., Pan, H., Huard, J., & Li, Y. (2011) · PLoS ONE · doi:10.1371/journal.pone.0016699 · Cited by 31

Deletion of DNA ligase IV homolog confers higher gene targeting efficiency on homologous recombination in Komagataella phaffii

Ito, Y., Watanabe, T., Aikawa, S., Nishi, T., Nishiyama, T., Nakamura, Y., Hasunuma, T., Okubo, Y., Ishii, J., & Kondo, A. (2018) · FEMS Yeast Research · doi:10.1093/femsyr/foy074 · Cited by 31

A self-deleting Cre–lox–ermAM cassette, Cheshire, for marker-less gene deletion in Streptococcus pneumoniae

Weng, L., Biswas, I., & Morrison, D. A. (2009) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2009.10.007 · Cited by 31

Application of the FLP/FRT system for conditional gene deletion in yeast Saccharomyces cerevisiae

Park, Y., Masison, D. C., Eisenberg, E., & Greene, L. E. (2011) · Yeast · doi:10.1002/yea.1895 · Cited by 31

Lentiviral Vectors: A Powerful Tool to Target Astrocytes In Vivo

Delzor, A., Escartin, C., & Déglon, N. (2013) · Current Drug Targets · doi:10.2174/13894501113146660213 · Cited by 31

Neuron-specific expression of Cre recombinase during the late phase of brain development

Hirasawa, M., Cho, A., Sreenath, T., Sauer, B., Julien, J., & Kulkarni, A. B. (2001) · Neuroscience Research · doi:10.1016/s0168-0102(01)00216-4 · Cited by 31

Genetic Transformation of Filamentous Fungi: Achievements and Challenges

Lichius, A., Moreno-Ruiz, D., & Zeilinger, S. (2020) · Grand challenges in biology and biotechnology · doi:10.1007/978-3-030-29541-7_5 · Cited by 30

Engineered Minichromosomes in Plants

Birchler, J. A., Krishnaswamy, L., Gaeta, R. T., Masonbrink, R. E., & Zhao, C. (2010) · Critical Reviews in Plant Sciences · doi:10.1080/07352681003709918 · Cited by 29

The hemochromatosis protein HFE signals predominantly via the BMP type I receptor ALK3 in vivo

Traeger, L., Enns, C. A., Krijt, J., & Steinbicker, A. U. (2018) · Communications Biology · doi:10.1038/s42003-018-0071-1 · Cited by 29

A heterodimer of evolved designer-recombinases precisely excises a human genomic DNA locus

Lansing, F., Paszkowski‐Rogacz, M., Schmitt, L. T., Schneider, P. M., Romanos, T. R., Sonntag, J., & Buchholz, F. (2019) · Nucleic Acids Research · doi:10.1093/nar/gkz1078 · Cited by 29

Temporal requirement of dystroglycan glycosylation during brain development and rescue of severe cortical dysplasia via gene delivery in the fetal stage

Sudo, A., Kanagawa, M., Kondo, M., Ito, C., Kobayashi, K., Endo, M., Minami, Y., Aiba, A., & Toda, T. (2018) · Human Molecular Genetics · doi:10.1093/hmg/ddy032 · Cited by 29

New Plasmids for Fusarium Transformation Allowing Positive-Negative Selection and Efficient Cre-loxP Mediated Marker Recycling

Twaruschek, K., Spörhase, P., Michlmayr, H., Wiesenberger, G., & Adam, G. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.01954 · Cited by 28

The degree of polymerization and sulfation patterns in heparan sulfate are critical determinants of cytomegalovirus entry into host cells

Mitra, D., Hasan, M., Bates, J. T., Bierdeman, M. A., Ederer, D. R., Parmar, R. C., Fassero, L. A., Liang, Q., Qiu, H. Y., Tiwari, V., et al. (2021) · PLoS Pathogens · doi:10.1371/journal.ppat.1009803 · Cited by 30

Site-Specific DNA Excision in Transgenic Rice With a Cell-Permeable Cre Recombinase

Cao, M., Huang, J., Yao, Q., Liu, S., Wang, C., & Wei, Z. (2005) · Molecular Biotechnology · doi:10.1385/mb:32:1:055 · Cited by 28

Use of Site-Specific Recombination Systems in Plants

Odell, J. T., & Russell, S. H. (1994) · Springer eBooks · doi:10.1007/978-94-011-1094-5_11 · Cited by 28

HARO7 Encodes Chorismate Mutase of the Methylotrophic Yeast Hansenula polymorpha and Is Derepressed upon Methanol Utilization

Krappmann, S., Pries, R., Gellissen, G., Hiller, M., & Braus, G. H. (2000) · Journal of Bacteriology · doi:10.1128/jb.182.15.4188-4197.2000 · Cited by 28

A Modified Cre-lox Genetic Switch To Dynamically Control Metabolic Flow in Saccharomyces cerevisiae

Yamanishi, M., & Matsuyama, T. (2012) · ACS Synthetic Biology · doi:10.1021/sb200017p · Cited by 26

Cre reconstitution allows for DNA recombination selectively in dual-marker-expressing cells in transgenic mice

Xu, Y., Xu, G., Liu, B., & Gu, G. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkm559 · Cited by 27

Engineered bakers yeast as a sensitive bioassay indicator organism for the trichothecene toxin deoxynivalenol

Abolmaali, S., Mitterbauer, R., Oliver Spadiut, A., Peruci, M., Weindorfer, H., Lucyshyn, D., Ellersdorfer, G., Lemmens, M., Moll, W., & Adam, G. (2008) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2007.12.013 · Cited by 27

A Novel Vector for Construction of Markerless Multicopy Overexpression Transformants in Pichia pastoris

Li, D., Zhang, B., Li, S., Zhou, J., Cao, H., Huang, Y., & Cui, Z. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.01698 · Cited by 26

Interspersed expression of CUP-SHAPED COTYLEDON2 and REDUCED COMPLEXITY shapes Cardamine hirsuta complex leaf form

Bhatia, N., Wilson‐Sánchez, D., Strauss, S., Vuolo, F., Pieper, B., Hu, Z., Rambaud-Lavigne, L., & Tsiantis, M. (2023) · Current Biology · doi:10.1016/j.cub.2023.06.037 · Cited by 24

The long road to recombinase‐mediated plant transformation

Ow, D. W. (2015) · Plant Biotechnology Journal · doi:10.1111/pbi.12472 · Cited by 26

SCRaMbLEing to understand and exploit structural variation in genomes

Steensels, J., Gorkovskiy, A., & Verstrepen, K. J. (2018) · Nature Communications · doi:10.1038/s41467-018-04308-3 · Cited by 26

NLR-1/CASPR Anchors F-Actin to Promote Gap Junction Formation

Meng, L., & Yan, D. (2020) · Developmental Cell · doi:10.1016/j.devcel.2020.10.020 · Cited by 22

Deletion of Aspergillus nidulans aroC using a novel blaster module that combines ET cloning and marker rescue

Krappmann, S., & Braus, G. H. (2003) · Molecular Genetics and Genomics · doi:10.1007/s00438-002-0789-8 · Cited by 25

Energy metabolism and proliferation in pancreatic carcinogenesis

Regel, I., Kong, B., Raulefs, S., Erkan, M., Michalski, C., Hartel, M., & Kleeff, J. (2012) · Langenbeck's archives of surgery (Print) · doi:10.1007/s00423-012-0933-9 · Cited by 25

The Use of Transgenic Mouse Models in the Study of Male Infertility

Tamowski, S., Aston, K. I., & Carrell, D. T. (2010) · Systems Biology in Reproductive Medicine · doi:10.3109/19396368.2010.485244 · Cited by 27

Isolation of yeast artificial chromosomes free of endogenous yeast chromosomes: construction of alternate hosts with defined karyotypic alterations.

Hamer, L., Johnston, M., & Green, E. D. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.25.11706 · Cited by 24

amdS as a dominant recyclable marker in Cryptococcus neoformans

Erpf, P. E., Stephenson, C. J., & Fraser, J. A. (2019) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2019.103241 · Cited by 20

Transgenic mouse lines for non-invasive ratiometric monitoring of intracellular chloride

Batti, L., Mukhtarov, M. R., Audero, E., Ivanov, A., Paolicelli, R. C., Zurborg, S., Gross, C. T., Bregestovski, P. D., & Heppenstall, P. A. (2013) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2013.00011 · Cited by 23

Cre-mediated targeted gene activation in the middle silk glands of transgenic silkworms (Bombyx mori)

Duan, J., Xu, H., Ma, S., Guo, H., Wang, F., Zhao, P., & Xia, Q. (2012) · Transgenic Research · doi:10.1007/s11248-012-9677-0 · Cited by 23

Reversible impairment of the ku80 gene by a recyclable marker in Aspergillus aculeatus

Tani, S., Tsuji, A. B., Kunitake, E., Sumitani, J., & Kawaguchi, T. (2013) · AMB Express · doi:10.1186/2191-0855-3-4 · Cited by 23

Pseudohyphal growth in Saccharomyces cerevisiae involves protein kinase-regulated lipid flippases

Frøsig, M. M., Costa, S. R., Liesche, J., Østerberg, J. T., Hanisch, S., Nintemann, S. J., Sørensen, H., Palmgren, M. B., Pomorski, T. G., & López‐Marqués, R. L. (2020) · Journal of Cell Science · doi:10.1242/jcs.235994 · Cited by 22

Going green in Cryptococcus neoformans: The recycling of a selectable drug marker

Patel, R. D., Lodge, J. K., & Baker, L. G. (2009) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2009.11.007 · Cited by 22

cDNA expression cloning in human cells using the pλDR2 episomal vector system

Murphy, A. J., Kung, A. L., Swirski, R. A., & SCHIMKE, R. T. (1992) · Methods · doi:10.1016/1046-2023(92)90044-9 · Cited by 22

A Tn3 derivative that can be used to make short in-frame insertions within genes.

Hoekstra, M. F., Burbee, D., Singer, J. D., Mull, E., Chiao, E. T., & Heffron, F. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.12.5457 · Cited by 22

TKO'ed: Lox, stock and barrel

Chambers, C. A. (1994) · BioEssays · doi:10.1002/bies.950161202 · Cited by 22

High frequency recombination betweenloxP sites in human chromosomes mediated by an adenovirus vector expressing Cre recombinase

Wang, P., Anton, M., Graham, F. L., & Bacchetti, S. (1995) · Somatic Cell and Molecular Genetics · doi:10.1007/bf02310209 · Cited by 22

Use and Visualization of Neuroanatomical Viral Transneuronal Tracers

Card, J. P., & Enquist, L. W. (2012) · Neuromethods · doi:10.1007/978-1-61779-897-9_11 · Cited by 21

Deciphering neural heterogeneity through cell lineage tracing

Figueres‐Oñate, M., Sánchez‐González, R., & López‐Mascaraque, L. (2020) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-020-03689-3 · Cited by 20

A Novel Malaria Vaccine Candidate Antigen Expressed in Tetrahymena thermophila

Cowan, G., Bockau, U., Eleni-Muus, J., Aldag, I., Samuel, K., Creasey, A., Hartmann, M., & Cavanagh, D. (2014) · PLoS ONE · doi:10.1371/journal.pone.0087198 · Cited by 17

Controlling feeding behavior by chemical or gene-directed targeting in the brain: what's so spatial about our methods?

Khan, A. M. (2013) · Frontiers in Neuroscience · doi:10.3389/fnins.2013.00182 · Cited by 22

ALK3 undergoes ligand-independent homodimerization and BMP-induced heterodimerization with ALK2

Traeger, L., Gallitz, I., Sekhri, R., Bäumer, N., Kuhlmann, T., Kemming, C., Holtkamp, M., Müller, J.-C., Kärst, U., Canonne‐Hergaux, F., et al. (2018) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2018.09.021 · Cited by 21

Systematic optimization and prediction of cre recombinase for precise genome editing in mice

Erhardt, V., Hartig, E. I., Lorenzo, K., Megathlin, H. R., Tarchini, B., & Hosur, V. (2025) · Genome biology · doi:10.1186/s13059-025-03560-3 · Cited by 24

A Vector–Host System to Fingerprint Virus Tropism

Hillestad, M. L., Guenzel, A. J., Nath, K. A., & Barry, M. A. (2012) · Human Gene Therapy · doi:10.1089/hum.2011.116 · Cited by 22

Updates on Approaches for Studying Atherosclerosis

Wu, C., Daugherty, A., & Lu, H. S. (2019) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.119.312001 · Cited by 21

Site-specific recombination of asymmetric lox sites mediated by a heterotetrameric Cre recombinase complex

Saraf-Levy, T., Santoro, S. W., Volpin, H., Kushnirsky, T., Eyal, Y., Schultz, P. G., Gidoni, D., & Carmi, N. (2006) · Bioorganic & Medicinal Chemistry · doi:10.1016/j.bmc.2005.12.016 · Cited by 20

E-cadherin cytoplasmic domain inhibits cell surface localization of endogenous cadherins and fusion of C2C12 myoblasts

Ozawa, M. (2015) · Biology Open · doi:10.1242/bio.013938 · Cited by 20

Inhibition of recombinant human immunodeficiency virus type 1 replication by a site-specific recombinase

Flowers, C. C., Woffendin, C., Petryniak, J., Yang, S., & Nabel, G. J. (1997) · Journal of Virology · doi:10.1128/jvi.71.4.2685-2692.1997 · Cited by 20

Inducible expression based on regulated recombination: a single vector strategy for stable expression in cultured cells

Angrand, P. (1998) · Nucleic Acids Research · doi:10.1093/nar/26.13.3263 · Cited by 20

Modifiable Gene Expression in Mice: Kidney-Specific Deletion of a Target Gene via the Cre-loxP System

Stec, D. E., & Sigmund, C. D. (1998) · Nephron Experimental Nephrology · doi:10.1159/000020573 · Cited by 20

Genetic surgery in fungi: employing site-specific recombinases for genome manipulation

Krappmann, S. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-013-5480-y · Cited by 19

The lipid head group is the key element for substrate recognition by the P4 ATPase ALA2: a phosphatidylserine flippase

Theorin, L., Faxén, K., Sørensen, D. M., Migotti, R., Dittmar, G., Schiller, J., Daleke, D. L., Palmgren, M. B., López‐Marqués, R. L., & Pomorski, T. G. (2019) · Biochemical Journal · doi:10.1042/bcj20180891 · Cited by 19

Self-excising integrative yeast plasmid vectors containing an intronated recombinase gene

Agaphonov, M. O., & Alexandrov, A. I. (2014) · FEMS Yeast Research · doi:10.1111/1567-1364.12197 · Cited by 19

Cytochemical observation of regulated bacterial beta-galactosidase gene expression in mammalian cells.

Liu, H., Feliciano, E., & Stambrook, P. J. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.24.9951 · Cited by 19

Clinical trials and promising preclinical applications of CRISPR/Cas gene editing

Çerçi, B., Uzay, İ. A., Kara, M. K., & Dinçer, P. (2022) · Life Sciences · doi:10.1016/j.lfs.2022.121204 · Cited by 19

A CUC1/auxin genetic module links cell polarity to patterned tissue growth and leaf shape diversity in crucifer plants

Hu, Z., Wilson‐Sánchez, D., Bhatia, N., Rast-Somssich, M. I., Wu, A., Vlad, D., McGuire, L., Nikolov, L. A., Laufs, P., Gan, X., et al. (2024) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2321877121 · Cited by 20

Loss of CASK Accelerates Heart Failure Development

Mustroph, J., Sag, C. M., Bähr, F., Schmidtmann, A.-L., Gupta, S. N., Dietz, A., Islam, M. M. T., Lücht, C., Beuthner, B. E., Pabel, S., et al. (2021) · Circulation Research · doi:10.1161/circresaha.120.318170 · Cited by 18

Apln-CreERT:mT/mG reporter mice as a tool for sprouting angiogenesis study

Pi, J., Cheng, Y., Sun, H., Chen, X., Zhuang, T., Liu, J., Li, Y., Chang, H. J., Zhang, L., Zhang, Y., et al. (2017) · BMC Ophthalmology · doi:10.1186/s12886-017-0556-6 · Cited by 18

μXRF and LA-ICP-TQMS for quantitative bioimaging of iron in organ samples of a hemochromatosis model

Müller, J.-C., Horstmann, M., Traeger, L., Steinbicker, A. U., Sperling, M., & Kärst, U. (2018) · Journal of Trace Elements in Medicine and Biology · doi:10.1016/j.jtemb.2018.12.012 · Cited by 18

Expression and regulation of Runx2/Cbfa1 and osteoblast phenotypic markers during the growth and differentiation of human osteoblasts

Prince, M., Banerjee, C., Javed, A., Green, J., Lian, J. B., Stein, G. S., Bodine, P. V. N., & Komm, B. S. (2000) · Journal of Cellular Biochemistry · doi:10.1002/1097-4644(20010301)80:3 · Cited by 182

Rat embryonic stem cells create new era in development of genetically manipulated rat models.

Kawaharada, K., Kawamata, M., & Ochiya, T. (2015) · PubMed Central · doi:10.4252/wjsc.v7.i7.1054 · Cited by 17

Timely Wound Healing Is Dependent on Endothelial but Not on Hair Follicle Stem Cell Toll-Like Receptor 2 Signaling

Xiong, L., McCoy, M. G., Murtazina, R., Podrez, E. A., & Byzova, T. V. (2022) · Journal of Investigative Dermatology · doi:10.1016/j.jid.2022.04.018 · Cited by 17

Bioluminescence imaging of Arc expression enables detection of activity-dependent and plastic changes in the visual cortex of adult mice

Izumi, H., Ishimoto, T., Yamamoto, H., Nishijo, H., & Mori, H. (2010) · Brain Structure and Function · doi:10.1007/s00429-010-0297-2 · Cited by 17

Puromycin‐ and methotrexate‐resistance cassettes and optimized Cre‐recombinase expression plasmids for use in yeast

MacDonald, C., & Piper, R. C. (2015) · Yeast · doi:10.1002/yea.3069 · Cited by 16

Enterococcus faeciumTIR-Domain Genes Are Part of a Gene Cluster Which Promotes Bacterial Survival in Blood

Wagner, T. M., Janice, J., Paganelli, F. L., Willems, R. J. L., Askarian, F., Pedersen, T., Top, J., de Haas, C. J. C., van Strijp, J. A. G., Johannessen, M., et al. (2018) · International Journal of Microbiology · doi:10.1155/2018/1435820 · Cited by 17

CORP: Using transgenic mice to study skeletal muscle physiology

Mobley, C. B., Vechetti, I. J., Valentino, T. R., & McCarthy, J. J. (2020) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00021.2020 · Cited by 16

Genetic approaches to retinal research in zebrafish

Niklaus, S., & Neuhauss, S. C. F. (2017) · Journal of Neurogenetics · doi:10.1080/01677063.2017.1343316 · Cited by 19

Application of Cre-lox gene switch to limit the Cry expression in rice green tissues

Chen, H., Luo, J., Peng, Z., Zhang, X., Zhang, C., Li, X., Wang, M., Huang, Y., Liu, X., Jan, M., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-14679-0 · Cited by 16

Strategies for the Genetic Manipulation of Saccharomyces cerevisiae

Tuite, M. F. (1992) · Critical Reviews in Biotechnology · doi:10.3109/07388559209069191 · Cited by 16

Chromosome Manipulation by Cre‐loxRecombination

Sauer, B. (2007) · doi:10.1128/9781555817954.ch4 · Cited by 16

Genetically modified mice—successes and failures of a widely used technology

Castrop, H. (2010) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/s00424-009-0770-z · Cited by 15

A Biobrick Library for Cloning Custom Eukaryotic Plasmids

Constante, M., Grünberg, R., & Isalan, M. (2011) · PLoS ONE · doi:10.1371/journal.pone.0023685 · Cited by 15

Functional expression of Cre recombinase in sub‐regions of mouse CNS and retina

Eriksson, B., Bergqvist, I., Eriksson, M., & Holmberg, D. (2000) · FEBS Letters · doi:10.1016/s0014-5793(00)01893-7 · Cited by 15

New Insights into the Pathomechanisms of Contact Dermatitis by the Use of Transgenic Mouse Models

Traidl‐Hoffmann, C., Merk, H. F., Cavani, A., & Hunzelmann, N. (2000) · Skin Pharmacology and Physiology · doi:10.1159/000029938 · Cited by 15

In vivo models of pancreatic ductal adenocarcinoma

Vudatha, V., Herremans, K. M., Freudenberger, D. C., Liu, C., & Trevino, J. (2023) · Advances in cancer research · doi:10.1016/bs.acr.2023.02.002 · Cited by 14

Optimization of the l-tyrosine metabolic pathway in Saccharomyces cerevisiae by analyzing p-coumaric acid production

Li, Y., Mao, J., Song, X., Wu, Y., Cai, M., Wang, H., Liu, Q., Zhang, X., Bai, Y., Xu, H., et al. (2020) · 3 Biotech · doi:10.1007/s13205-020-02223-3 · Cited by 14

Conditional removal of selectable markers in Trypanosoma cruzi using a site-specific recombination tool: Proof of concept

Kangussu-Marcolino, M. M., da Cunha, A. P., Ávila, A. R., Herman, J., & DaRocha, W. D. (2014) · Molecular and Biochemical Parasitology · doi:10.1016/j.molbiopara.2015.01.001 · Cited by 14

Utilizing GCaMP transgenic mice to monitor endogenous Gq/11-coupled receptors

Partridge, J. G. (2015) · Frontiers in Pharmacology · doi:10.3389/fphar.2015.00042 · Cited by 14

Technologies for the study of epididymal-specific genes

Lye, R. J., & Hinton, B. T. (2003) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2003.10.072 · Cited by 14

Development of high-titer retroviral producer cell lines by using Cre-mediated recombination

Vanin, E. F., Cerruti, L., Tran, N., Grosveld, G. C., Cunningham, J. M., & Jane, S. M. (1997) · Journal of Virology · doi:10.1128/jvi.71.10.7820-7826.1997 · Cited by 14

Expanded FLP toolbox for spatiotemporal protein degradation and transcriptomic profiling in Caenorhabditis elegans

Fragoso-Luna, A., Romero-Bueno, R., Eibl, M., Ayuso, C., Muñoz-Jiménez, C. M., Beneš, V., Cases, I., & Askjaer, P. (2022) · Genetics · doi:10.1093/genetics/iyac166 · Cited by 12

Heterogeneity and genomic loci of ubiquitous transgenic Cre reporter lines in zebrafish

Lalonde, R. L., Kemmler, C. L., Riemslagh, F. W., Aman, A. J., Kresoja‐Rakic, J., Moran, H. R., Nieuwenhuize, S., Parichy, D. M., Burger, A., & Mosimann, C. (2022) · Developmental Dynamics · doi:10.1002/dvdy.499 · Cited by 13

Conditional gene expression in invertebrate animal models

Driesschaert, B., Mergan, L., & Temmerman, L. (2021) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2021.01.005 · Cited by 12

A LacI-Family Regulator Activates Maltodextrin Metabolism of Enterococcus faecium

Zhang, X., Rogers, M. R. C., Bierschenk, D., Bonten, M. J. M., Willems, R. J. L., & van Schaik, W. (2013) · PLoS ONE · doi:10.1371/journal.pone.0072285 · Cited by 13

Design of a novel system for the construction of vectors for Agrobacterium-mediated plant transformation

Mozo, T., & Hooykaas, P. J. J. (1992) · Molecular and General Genetics MGG · doi:10.1007/bf00279636 · Cited by 13

Transneuronal Circuit Analysis with Pseudorabies Viruses

Engel, E. A., Card, J. P., & Enquist, L. W. (2023) · Current Protocols · doi:10.1002/cpz1.841 · Cited by 12

Trypanosoma brucei: a first-generation CRE-loxP site-specific recombination system

Barrett, B., LaCount, D. J., & Donelson, J. E. (2004) · Experimental Parasitology · doi:10.1016/j.exppara.2004.01.004 · Cited by 12

Principles of Genetic Engineering

Oliveira, C., Aguiar, T. Q., & Domingues, L. (2016) · Elsevier eBooks · doi:10.1016/b978-0-444-63668-3.00004-4 · Cited by 13

Synthetic biology approaches in cancer immunotherapy, genetic network engineering, and genome editing

Chakravarti, D., Cho, J. H., Weinberg, B. H., Wong, N. M. L., & Wong, W. W. (2016) · Integrative Biology · doi:10.1039/c5ib00325c · Cited by 13

Promoter Cre‐Specific Genotyping Assays for Authentication of Cre‐Driver Mouse Lines

Couasnay, G., Frey, C., & Elefteriou, F. (2018) · JBMR Plus · doi:10.1002/jbm4.10128 · Cited by 11

Toxic and Phenotypic Effects of AAV_Cre Used to Transduce Mesencephalic Dopaminergic Neurons

Erben, L., Welday, J. P., Murphy, R., & Buonanno, A. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23169462 · Cited by 11

Site-specific integration of transgene targeting an endogenous lox-like site in early mouse embryos

Ito, M., Yamanouchi, K., Naito, K., Calos, M. P., & Tojo, H. (2010) · Journal of Applied Genetics · doi:10.1007/s13353-010-0011-3 · Cited by 11

Technology used to build and transfer mammalian chromosomes

Brown, D., & Glass, J. I. (2020) · Experimental Cell Research · doi:10.1016/j.yexcr.2020.111851 · Cited by 11

Genetic Recombination as a Reporter for Screening Steroid Receptor Agonists and Antagonists

Dias, J., Go, N. F., Hart, C. P., & Mattheakis, L. C. (1998) · Analytical Biochemistry · doi:10.1006/abio.1998.2583 · Cited by 11

Recombinators, recombinases and recombination genes of yeasts

Esposito, M. S., Ramirez, R. M., & Bruschi, C. V. (1994) · Current Genetics · doi:10.1007/bf00712959 · Cited by 11

Advances in large-scale DNA engineering with the CRISPR system

Gwon, L. W., Badon, I. W., Lee, Y., Kim, H., & Lee, S. H. (2025) · Experimental & Molecular Medicine · doi:10.1038/s12276-025-01530-0 · Cited by 12

Towards a Cre-based recombination system for generating integrated DNA repertoires site-specifically in yeast

Davies, B. G., Adams, C., Aijaz, S., & Cox, J. P. (2002) · Biotechnology Letters · doi:10.1023/a:1015246420023 · Cited by 3

Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase.

Sauer, B., & Henderson, N. (1990) · PubMed · Cited by 259

Cre/lox P系统介导的基因重组及其在酵母中的应用

和东芹, 肖冬光, & 吕烨 (2008)

Chromosome engineering in Saccharomyces cerevisiae by using a site-specific recombination system of a yeast plasmid

Matsuzaki, H., Nakajima, R., Nishiyama, J., Araki, H., & Oshima, Y. (1990) · Journal of Bacteriology · doi:10.1128/jb.172.2.610-618.1990 · Cited by 104

Identification of cryptic lox sites in the yeast genome by selection for Cre-mediated chromosome translocations that confer multiple drug resistance

Sauer, B. (1992) · Journal of Molecular Biology · doi:10.1016/0022-2836(92)90252-f · Cited by 78