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RNA polymerase II subunit RPB4 is essential for high- and low-temperature yeast cell growth.
Open accessAbstract
RPB4 encodes the fourth-largest RNA polymerase II subunit in Saccharomyces cerevisiae. The RPB4 gene was cloned and sequenced, and its identity was confirmed by amino acid sequence analysis of tryptic peptides from the purified subunit. The RPB4 DNA sequence predicted a protein of 221 amino acids with a molecular mass of 25,414 daltons. The central 100 amino acids of the RPB4 protein were found to be similar to a segment of the major sigma subunit in Escherichia coli RNA polymerase. Deletion of RPB4 produced cells that were heat and cold sensitive but could grow, albeit slowly, at intermediate temperatures. RNA polymerase II lacking the RPB4 subunit exhibited markedly reduced activity in crude extracts in vitro. The RPB4 subunit, although not essential for mRNA synthesis or enzyme assembly, was essential for normal levels of RNA polymerase II activity and indispensable for cell viability over a wide temperature range.
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Cited by
Rpb4 and Rpb9 mediate subpathways of transcription‐coupled DNA repair in Saccharomyces cerevisiae
Li, S., & Smerdon, M. J. (2002) · The EMBO Journal · doi:10.1093/emboj/cdf589 · Cited by 137
Metadata
- Authors
- Woychik, N. A., & Young, R. A.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 1989-07
- Volume
- 9
- Issue
- 7
- Pages
- 2854-2859
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- OpenAlex
- W1911817655
- Cited by
- 178
- References
- 0
- FWCI
- 4.09
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: RNA polymerase II subunit RPB4 is essential for high- and low-temperature yeast cell growth. https://app.cochat.ai/papers/10.1128/mcb.9.7.2854
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- Woychik, N. A., & Young, R. A. (1989). RNA polymerase II subunit RPB4 is essential for high- and low-temperature yeast cell growth. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.9.7.2854
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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