Rpb4 and Rpb9 mediate subpathways of transcription‐coupled DNA repair in Saccharomyces cerevisiae
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Rpb9, a non-essential subunit of RNA polymerase II, mediates a transcription-coupled repair (TCR) subpathway in Saccharomyces cerevisiae. This subpathway initiates at the same upstream site as the previously identified Rad26 subpathway. However, the Rpb9 subpathway operates more effectively in the coding region than in the region upstream of the transcription start site, whereas the Rad26 subpathway operates equally in the two regions. Rpb4, another non-essential subunit of RNA polymerase II, plays a dual role in regulating the two subpathways, suppressing the Rpb9 subpathway and facilitating the Rad26 subpathway. Simultaneous deletion of RPB9 and RAD26 genes completely abolishes TCR in both the coding and upstream regions, indicating that no other TCR subpathway exists in RNA polymerase II-transcribed genes.
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Budding yeast has a second TC-NER pathway that is dependent on Rpb9, a nonessential subunit of RNAPII.
“In addition, budding yeast has a second TC-NER pathway, which is dependent on Rpb9, a nonessential subunit of RNAPII (29).”
From A Role for Checkpoint Kinase-Dependent Rad26 Phosphorylation in Transcription-Coupled DNA Repair in <i>Saccharomyces cerevisiae</i> (2010) · cites this paper as [29]
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- Authors
- Li, S., & Smerdon, M. J.
- Affiliations
- Washington State University (US)
- Corresponding author
- Smerdon, Michael J.
- ORCID
- Li, Shisheng
- Venue
- The EMBO Journal
- Published
- 2002-11-01
- Volume
- 21
- Issue
- 21
- Pages
- 5921-5929
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC131086
- OpenAlex
- W2046195682
- Cited by
- 137
- References
- 64
- Citations by year
- 2026: 4 · 2024: 6 · 2023: 7 · 2022: 1 · 2021: 10 · 2020: 9 · 2019: 4 · 2018: 4 · 2017: 2 · 2016: 8 · 2015: 3 · 2014: 8 · 2013: 6 · 2012: 5
- FWCI
- 2.09
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The EMBO Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (ES04106)
- National Institute of Environmental Health Sciences (ES04106, R01ES004106)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- transcription-coupled repair, Rpb4, Rad26, RNA polymerase II, Saccharomyces cerevisiae, DNA damage tolerance, transcription start sites
- MeSH terms
- DNA Repair, DNA, Fungal, RNA Polymerase II, Saccharomyces cerevisiae, Transcription, Genetic, Blotting, Northern, Saccharomyces cerevisiae Proteins, Schizosaccharomyces pombe Proteins
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