Cited by 137DNA Repair MechanismsFungal and yeast genetics researchGenomics and Chromatin Dynamics

Rpb4 and Rpb9 mediate subpathways of transcription‐coupled DNA repair in Saccharomyces cerevisiae

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Li, Shisheng, Smerdon, Michael J.·2002·The EMBO Journal

Abstract

Rpb9, a non-essential subunit of RNA polymerase II, mediates a transcription-coupled repair (TCR) subpathway in Saccharomyces cerevisiae. This subpathway initiates at the same upstream site as the previously identified Rad26 subpathway. However, the Rpb9 subpathway operates more effectively in the coding region than in the region upstream of the transcription start site, whereas the Rad26 subpathway operates equally in the two regions. Rpb4, another non-essential subunit of RNA polymerase II, plays a dual role in regulating the two subpathways, suppressing the Rpb9 subpathway and facilitating the Rad26 subpathway. Simultaneous deletion of RPB9 and RAD26 genes completely abolishes TCR in both the coding and upstream regions, indicating that no other TCR subpathway exists in RNA polymerase II-transcribed genes.

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  1. Sambrook,J. and Russell,D.W. (2001) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

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Authors
Li, S., & Smerdon, M. J.
Affiliations
Washington State University (US)
Corresponding author
Smerdon, Michael J.
Venue
The EMBO Journal
Published
2002-11-01
Volume
21
Issue
21
Pages
5921-5929
ISSN
  • 0261-4189 (ISSN-L)
  • 1460-2075
OpenAlex
W2046195682
Cited by
137
References
64
Citations by year
2026: 4 · 2024: 6 · 2023: 7 · 2022: 1 · 2021: 10 · 2020: 9 · 2019: 4 · 2018: 4 · 2017: 2 · 2016: 8 · 2015: 3 · 2014: 8 · 2013: 6 · 2012: 5
FWCI
2.09
Type
journal article
Language
en
Open access
Locations
  • The EMBO Journal (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (ES04106)
  • National Institute of Environmental Health Sciences (ES04106, R01ES004106)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
transcription-coupled repair, Rpb4, Rad26, RNA polymerase II, Saccharomyces cerevisiae, DNA damage tolerance, transcription start sites
MeSH terms
DNA Repair, DNA, Fungal, RNA Polymerase II, Saccharomyces cerevisiae, Transcription, Genetic, Blotting, Northern, Saccharomyces cerevisiae Proteins, Schizosaccharomyces pombe Proteins

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