Iron Acquisition and Siderophore Release by Carbapenem-Resistant Sequence Type 258 Klebsiella pneumoniae
Open accessAbstract
Carbapenem-resistant Enterobacteriaceae , including K. pneumoniae , are a major health care concern worldwide because they cause a wide range of infection and are resistant to all or nearly all antibiotics. To cause infection, these bacteria must acquire iron, and a major mechanism of acquiring iron is by secreting a molecule called enterobactin that strips iron from host proteins. However, a subset of carbapenem-resistant K. pneumoniae strains that lack a portion of the entS gene that is required for enterobactin secretion was recently discovered. To understand how these mutant strains obtain iron, we studied their transcriptional responses, bacterial growth, and enterobactin secretion under iron-limited conditions. We found that strains both with mutated and intact entS genes grow under iron-limiting conditions, secrete enterobactin, and utilize an alternate iron source, hemin, for growth. Our data indicate that carbapenem-resistant K. pneumoniae can use varied methods for iron uptake during infection.
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The elaboration and acquisition of the latter three siderophores are associated with the pathogenesis and hypervirulence of K. pneumoniae.
“The elaboration and acquisition of the latter three siderophores are associated with the pathogenesis and hypervirulence of K. pneumoniae (1,44,53,54).”
From Siderophore-mediated iron acquisition by <i>Klebsiella pneumoniae</i> (2024) · cites this paper as [53]
The Discussion (page unavailable) says iron acquisition is essential for K. pneumoniae virulence. The Results (page unavailable) also report that salmochelin supports growth in serum, and the References list a study linking yersiniabactin to respiratory infection. Our copy does not establish that elaboration and acquisition of all three siderophores are associated with hypervirulence. (LLM, conf=0.78)
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- Authors
- Holden, V. I., Wright, M. S., Houle, S., Collingwood, A., Dozois, C. M., Adams, M. D., & Bachman, M. A.
- Affiliations
- University of Michigan (US)
- J. Craig Venter Institute (US)
- Institut National de la Recherche Scientifique (CA)
- Corresponding author
- Bachman, Michael A.
- Venue
- mSphere
- Publisher
- American Society for Microbiology
- Published
- 2018-04-25
- Volume
- 3
- Issue
- 2
- Pages
- e00125-18
- ISSN
- 2379-5042 (ISSN-L)
- PMC
- PMC5907654
- OpenAlex
- W2801652714
- Cited by
- 42
- References
- 32
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 6 · 2023: 4 · 2022: 2 · 2021: 11 · 2020: 6 · 2019: 4
- FWCI
- 1.99
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- mSphere (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- EspaceINRS (National Institute for Scientific Research (Canada)) (submittedVersion)
- HAL (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- National Sciences and Engineering Research Council of Canada (Discovery Grant)
- HHS | National Institutes of Health (U19AI110819)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Enterobacteriaceae and Cronobacter Research
- Keywords
- Klebsiella pneumoniae, carbapenem resistance, enterobactin, iron acquisition, iron limitation, transcriptional response, antibiotic resistance, virulence
- MeSH terms
- Hemin, Humans, Iron, Klebsiella pneumoniae, Carbapenems, Genome, Bacterial, Siderophores, Drug Resistance, Bacterial, Multilocus Sequence Typing, Transcriptome
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