Angiotensin converting enzyme in human synovium: increased stromal [125I]351A binding in rheumatoid arthritis
Open accessAbstract
Objective To determine whether tissue angiotensin converting enzyme (ACE) is increased in synovia from patients with rheumatoid arthritis, osteoarthritis or chondromalacia patellae.Methods Sections of synovia from patients with rheumatoid arthritis (n = 7), osteoarthritis (n = 7) or chondromalacia patellae (n = 6) were tested for immunoreactivity for ACE, and for binding of the ACE inhibitor [(125)I]351A. The amount of ACE was measured with computer assisted image analysis as the proportion of synovial section area occupied by ACE-immunoreactive cells, and the density of [(125)I]351A binding.Results [(125)I]351A binding sites had characteristics of ACE and colocalised with ACE-like immunoreactivity to microvascular endothelium and fibroblast-like stromal cells in inflamed and non-inflamed human synovium. Stromal [(125)I]351A binding densities (B(eq)) and the fraction of synovial section area occupied by ACE-immunoreactivity (fractional area) were higher in synovia from patients with rheumatoid arthritis (B(eq) 28 amol/mm(2), fractional area 0.21) than from those with osteoarthritis (B(eq) 9 amol/mm(2), fractional area 0.10) or chondromalacia patellae (B(eq) 9 amol/mm(2), fractional area 0.09)(p < 0.05). Density of [(125)I]351A binding to stroma was similar to that to blood vessels in rheumatoid arthritis, but less dense than vascular binding in chondromalacia patellae and osteoarthritis. Increases in [(125)I]351A binding densities were attributable to increases in the numbers of binding sites, and were consistent with an increase in the density of ACE bearing stromal cells.Conclusion ACE is upregulated in synovial stroma in rheumatoid arthritis. Increased tissue ACE may result in increased local generation of the vasoconstrictor and mitogenic peptide angiotensin II and thereby potentiate synovial hypoxia and proliferation in rheumatoid arthritis.
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ACE is upregulated in synovial tissue of patients with rheumatoid arthritis, leading to higher availability of angiotensin II in inflamed joints.
“ACE is upregulated in synovial tissue of patients with RA, leading to higher availability of angiotensin II in inflamed joints [144].”
From Interaction of the endocrine system with inflammation: a function of energy and volume regulation (2014) · cites this paper as [144]
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Interaction of the endocrine system with inflammation: a function of energy and volume regulation
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“ACE is upregulated in synovial tissue of patients with RA, leading to higher availability of angiotensin II in inflamed joints [144].”
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- Authors
- Walsh, D. A., Catravas, J., & Wharton, J.
- Affiliations
- University of Nottingham (GB)
- Corresponding author
- Walsh, David Andrew
- Venue
- Annals of the Rheumatic Diseases
- Publisher
- Elsevier BV
- Published
- 2000-02
- Volume
- 59
- Issue
- 2
- Pages
- 125-131
- ISSN
- 0003-4967 (ISSN-L)
- OpenAlex
- W2101924426
- Cited by
- 57
- References
- 45
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 4 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 4 · 2018: 5 · 2016: 5 · 2015: 7 · 2014: 3 · 2013: 3
- FWCI
- 0.23
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Annals of the Rheumatic Diseases (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Topics
- Health Sciences › Medicine › Rheumatology › Rheumatoid Arthritis Research and Therapies
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Renin-Angiotensin System Studies
- Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
- Keywords
- angiotensin-converting enzyme, synovium, rheumatoid arthritis, osteoarthritis, chondromalacia patellae, angiotensin II, synovitis, microvascular endothelium, enzyme up-regulation, cell proliferation
- MeSH terms
- Adult, Aged, Angiotensin-Converting Enzyme Inhibitors, Arthritis, Rheumatoid, Cartilage Diseases, Dipeptides, Female, Humans, Immunoenzyme Techniques, Iodine Radioisotopes, Peptidyl-Dipeptidase A, Male, Middle Aged, Osteoarthritis, Synovial Membrane, Stromal Cells
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