The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Reduced diversity of faecal microbiota in Crohn’s disease revealed by a metagenomic approach
Open accessAbstract
Background and aim: A role for the intestinal microbial community (microbiota) in the onset and chronicity of Crohn’s disease (CD) is strongly suspected. However, investigation of such a complex ecosystem is difficult, even with culture independent molecular approaches. Methods: We used, for the first time, a comprehensive metagenomic approach to investigate the full range of intestinal microbial diversity. We used a fosmid vector to construct two libraries of genomic DNA isolated directly from faecal samples of six healthy donors and six patients with CD. Bacterial diversity was analysed by screening the two DNA libraries, each composed of 25 000 clones, for the 16S rRNA gene by DNA hybridisation. Results: Among 1190 selected clones, we identified 125 non-redundant ribotypes mainly represented by the phyla Bacteroidetes and Firmicutes. Among the Firmicutes, 43 distinct ribotypes were identified in the healthy microbiota, compared with only 13 in CD (p<0.025). Fluorescent in situ hybridisation directly targeting 16S rRNA in faecal samples analysed individually (n = 12) confirmed the significant reduction in the proportion of bacteria belonging to this phylum in CD patients (p<0.02). Conclusion: The metagenomic approach allowed us to detect a reduced complexity of the bacterial phylum Firmicutes as a signature of the faecal microbiota in patients with CD. It also indicated the presence of new bacterial species.
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“The microbiome has long been implicated in IBD, potentially as a causative or risk factor66,67”
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Self-citationA metagenome-wide association study of gut microbiota in type 2 diabetes
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Metadata
- Authors
- Manichanh, C., Rigottier-Gois, L., Bonnaud, E., Gloux, K., Pelletier, E., Frangeul, L., Nalin, R., Jarrin, C., Chardon, P., Marteau, P., Roca, J., & Dore, J.
- Affiliations
- Institut National de la Recherche Agronomique (FR)
- Institut Pasteur (FR)
- Commissariat à l'Énergie Atomique et aux Énergies Alternatives (FR)
- Hôpital Européen Georges-Pompidou (FR)
- Consejo Superior de Investigaciones Científicas (ES)
- Corresponding author
- Dore, J
- Venue
- Gut
- Publisher
- BMJ
- Published
- 2005-09-27
- Volume
- 55
- Issue
- 2
- Pages
- 205-211
- ISSN
- 0017-5749 (ISSN-L)
- 1468-3288
- PMC
- PMC1856500
- OpenAlex
- W2156145269
- Cited by
- 2,277
- References
- 35
- Citations by year
- 2026: 35 · 2025: 76 · 2024: 100 · 2023: 114 · 2022: 134 · 2021: 198 · 2020: 172 · 2019: 157 · 2018: 148 · 2017: 127 · 2016: 143 · 2015: 145 · 2014: 148 · 2013: 111 · 2012: 107
- FWCI
- 16.50
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Gut (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- HAL (submittedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- Institut National de la Recherche Agronomique
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Gastroenterology › Inflammatory Bowel Disease
- Health Sciences › Medicine › Infectious Diseases › Clostridium difficile and Clostridium perfringens research
- Keywords
- Crohn's disease, gut microbiota, fecal microbiota, bacterial diversity, Firmicutes, Bacteroidetes, 16S rRNA gene, metagenomics, fosmid library, ribotypes, novel bacterial species, fluorescence in situ hybridization
- MeSH terms
- Adolescent, Adult, Bacteria, Crohn Disease, DNA, Bacterial, Feces, Female, Humans, Male, Phylogeny, RNA, Bacterial, RNA, Ribosomal, 16S, Bacterial Typing Techniques, Gene Library, In Situ Hybridization, Fluorescence, Ecosystem
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