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Differential expression of microRNAs in plasma of patients with colorectal cancer: a potential marker for colorectal cancer screening

Ng, E K O, Chong, W W S, Jin, H, Lam, E K Y, Shin, V Y, Yu, J, Poon, T C W, Ng, S S M +1 more·2009·Gut
DOI 10.1136/gut.2008.167817
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Abstract

Objective: MicroRNAs (miRNAs) have been shown to offer great potential in the diagnosis of cancer. We investigated whether plasma miRNAs could discriminate between patients with and without colorectal cancer (CRC). Methods: This study was divided into three phases: (1) marker discovery using real-time PCR-based miRNA profiling on plasma, corresponding cancerous and adjacent non-cancerous colonic tissues of five patients with CRC, along with plasma from five healthy individuals as controls; (2) marker selection and validation by real-time quantitative RT-PCR on a small set of plasma; and (3) independent validation on a large set of plasma from 90 patients with CRC, 20 patients with gastric cancer, 20 patients with inflammatory bowel disease (IBD) and 50 healthy controls. Results: Of the panel of 95 miRNAs analysed, five were upregulated both in plasma and tissue samples. All the five miRNAs were validated on the plasma of 25 patients with CRC and 20 healthy controls. Both miR-17-3p and miR-92 were significantly elevated in the patients with CRC (p<0.0005). The plasma levels of these markers were significantly reduced after surgery in 10 patients with CRC (p<0.05). Further validation with an independent set of plasma samples (n = 180) indicated that miR-92 differentiates CRC from gastric cancer, IBD and normal subjects. This marker yielded a receiver operating characteristic curve area of 88.5%. At a cut-off of 240 (relative expression in comparison to RNU6B snRNA), the sensitivity was 89% and the specificity was 70% in discriminating CRC from control subjects. Conclusion: MiR-92 is significantly elevated in plasma of patients with CRC and can be a potential non-invasive molecular marker for CRC screening.

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Crossref · 10.1136/gut.2008.167817

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  • Among the miRNA biomarkers examined, variation attributable to blood cell effects was greater in magnitude than many differences reported between cancer patients and controls.

    “we observed variation attributable to blood cell effects that was greater in magnitude than many of the differences reported between cancer patients and controls (13, 15, 20).”

    From Blood Cell Origin of Circulating MicroRNAs: A Cautionary Note for Cancer Biomarker Studies (2012) · cites this paper as [15]

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Authors
Ng, E. K. O., Chong, W. W. S., Jin, H., Lam, E. K. Y., Shin, V. Y., Yu, J., Poon, T. C. W., Ng, S. S. M., & Sung, J. J. Y.
Venue
Gut
Publisher
BMJ
Published
2009-02-06
Volume
58
Issue
10
Pages
1375-1381
ISSN
  • 0017-5749
  • 1468-3288
DOI
10.1136/gut.2008.167817
Cited by
936
References
0
Language
en

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