The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Helicobacter pylori induced interleukin-8 expression in gastric epithelial cells is associated with CagA positive phenotype.
Open accessAbstract
AIMS--To use a range of natural phenotypically variant strains of Helicobacter pylori with disparate CagA and VacA (vacuolating cytotoxin) expression to determine which bacterial factors are more closely associated with epithelial interleukin-8 (IL-8) induction. METHODS--Gastric epithelial cells (AGS and KATO-3) were co-cultured with five H pylori strains which were variously shown to express the cagA gene/CagA protein, VacA and/or to exhibit biological cytotoxicity. Secreted IL-8 was assayed by enzyme leaked immunosorbent assay (ELISA) and IL-8 messenger RNA (mRNA) was assayed using a reverse transcription polymerase chain reaction based technique (RT-PCR). RESULTS--Strains expressing CagA, including a variant strain (D931) which is non-cytotoxic and does not express the VacA protein, were found to upregulate epithelial IL-8 secretion and gene expression. In contrast, strains with no CagA expression, even in the presence of VacA and/or biological cytotoxicity, (G104, BA142), failed to induce IL-8 protein or mRNA above control values. CONCLUSIONS--These results strongly support a role for H pylori CagA or coexpressed factors other than the cytotoxin in upregulation of gastric epithelial IL-8. Increased epithelial IL-8 secretion and concomitant neutrophil chemotaxis and activation in addition to direct cytotoxicity may be an important factor in tissue damage and ulceration.
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Cites
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Cited by
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Lymphocytes in the human gastric mucosa during Helicobacter pylori have a T helper cell 1 phenotype
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A whole-genome microarray reveals genetic diversity among Helicobacter pylori strains
Salama, N., Guillemin, K., McDaniel, T. K., Sherlock, G., Tompkins, L., & Falkow, S. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.26.14668 · Cited by 559
Helicobacter pylori evolution and phenotypic diversification in a changing host
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Metadata
- Authors
- Crabtree, J. E., Covacci, A., Farmery, S. M., Xiang, Z., Tompkins, D. S., Perry, S., Lindley, I. J., & Rappuoli, R.
- Venue
- Journal of Clinical Pathology
- Publisher
- BMJ
- Published
- 1995-01
- Volume
- 48
- Issue
- 1
- Pages
- 41-45
- ISSN
- 0021-9746 (ISSN-L)
- 1472-4146
- OpenAlex
- W2022076163
- Cited by
- 349
- References
- 26
- FWCI
- 32.06
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Helicobacter pylori induced interleukin-8 expression in gastric epithelial cells is associated with CagA positive phenotype. https://app.cochat.ai/papers/10.1136/jcp.48.1.41
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- Crabtree, J. E., Covacci, A., Farmery, S. M., Xiang, Z., Tompkins, D. S., Perry, S., Lindley, I. J., & Rappuoli, R. (1995). Helicobacter pylori induced interleukin-8 expression in gastric epithelial cells is associated with CagA positive phenotype. Journal of Clinical Pathology. https://doi.org/10.1136/jcp.48.1.41
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
