Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 15,001–15,100 of 48,846

Desempenho, características de carcaça e qualidade da carne de bovinos superprecoces das raças Canchim, Nelore e seus mestiços
Rubiano, G. A. G., Arrigoni, M. D. B., Martins, C. L., et al. (2009) · Revista Brasileira de Zootecnia · doi:10.1590/s1516-35982009001200027 · Cited by 43
Very-long-chain saturated fatty acids in phosphatidylserine from higher plant tissues
Murata, N., Sato, N., & Takahashi, N. (1984) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(84)90115-2 · Cited by 43
Physicochemical and Biological Characterization of Ceramide-Containing Liposomes: Paving the Way to Ceramide Therapeutic Application
Khazanov, E., Priev, A., Shillemans, J. P., et al. (2008) · Langmuir · doi:10.1021/la800207z · Cited by 43
Positional distribution of fatty acids in galactolipids of algae
Arao, T., & Yamada, M. (1989) · Phytochemistry · doi:10.1016/0031-9422(89)80119-0 · Cited by 42
Trophodynamics and functional feeding groups of North Sea fauna: a combined stable isotope and fatty acid approach
Kürten, B., Frutos, I., Struck, U., Painting, S. J., Polunin, N. V. C., & Middelburg, J. J. (2012) · Biogeochemistry · doi:10.1007/s10533-012-9701-8 · Cited by 46
Assessing the effect of air-drying and storage on microbial biomass and community structure in paddy soils
Liu, Y., Yao, H., & Huang, C. (2008) · Plant and Soil · doi:10.1007/s11104-008-9803-1 · Cited by 43
Purification and characterization of human hemoglobin: effect of the hemolysis conditions
Andrade, P. B., Barros, L. A. M., Lima, M. C. P., et al. (2004) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2004.05.003 · Cited by 43
Biologically Active Lipids with Antiatherogenic Properties from White Wine and Must
Fragopoulou, E., Antonopoulou, S., & Demopoulos, C. A. (2002) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf011288u · Cited by 43
Incorporation of salmon fish feed and feces components in mussels (Mytilus edulis): Implications for integrated multi-trophic aquaculture in cool-temperate North Atlantic waters
Handå, A., Ranheim, A., Olsen, A. J., et al. (2012) · Aquaculture · doi:10.1016/j.aquaculture.2012.09.030 · Cited by 43
The effect of growth temperature on the phospholipid and fatty acyl compositions of non-proteolytic Clostridium botulinum
Evans, R. I., Mcclure, P. J., Gould, G. W., & Russell, N. J. (1998) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(98)00029-4 · Cited by 43
Transfection properties of stabilized plasmid–lipid particles containing cationic PEG lipids
Palmer, L., Chen, T., Lam, A. M. I., et al. (2003) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/s0005-2736(03)00058-0 · Cited by 43
Erythrocyte membrane docosapentaenoic acid levels are associated with islet autoimmunity: the Diabetes Autoimmunity Study in the Young
Norris, J. M., Kroehl, M., Fingerlin, T. E., Frederiksen, B. N., Seifert, J. A., Wong, R., Clare-Salzler, M. J., & Rewers, M. (2013) · Diabetologia · doi:10.1007/s00125-013-3106-7 · Cited by 43
Expression of the Streptococcus pneumoniae Type 3 Synthase in Escherichia coli
Cartee, R. T., Forsee, W. T., Jensen, J. W., et al. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m106481200 · Cited by 43
Cytosolic phospholipase A2 regulates viability of irradiated vascular endothelium
Yazlovitskaya, E. M., Linkous, A. G., Thotala, D. K., Cuneo, K. C., & Hallahan, D. E. (2008) · Cell Death and Differentiation · doi:10.1038/cdd.2008.93 · Cited by 43
Distribution and biosynthesis of stearidonic acid in leaves of Borago officinalis
Griffiths, G. J., Brechany, E. Y., Jackson, F. M., et al. (1996) · Phytochemistry · doi:10.1016/0031-9422(96)00305-6 · Cited by 43
A lipoxygenase with linoleate diol synthase activity from Nostoc sp. PCC 7120
Lang, I., Göbel, C., Porzel, A., et al. (2008) · Biochemical Journal · doi:10.1042/bj20071277 · Cited by 43
The effect of lufenuron, a chitin synthesis inhibitor, on oogenesis of Rhodnius prolixus
Mansur, J. F., Figueira-Mansur, J., dos Santos, A. S., et al. (2010) · Pesticide Biochemistry and Physiology · doi:10.1016/j.pestbp.2010.04.013 · Cited by 43
Concomitant Acceleration of the Activation and Inactivation Kinetics of the Human Delayed Rectifier K+ Channel (Kv1.1) by Ca2+-independent Phospholipase A2
Gubitosi‐Klug, R., Yu, S. P., Choi, D. W., et al. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.7.2885 · Cited by 43
Characterization of native and oxidized human low-density lipoproteins by the Z-scan technique
Gómez, S. L., Turchiello, R. F., Jurado, M. C., et al. (2004) · Chemistry and Physics of Lipids · doi:10.1016/j.chemphyslip.2004.07.001 · Cited by 43
Comparison of Pond Production Efficiency, Fatty Acid Profiles, and Contaminants in Litopenaeus vannamei Fed Organic Plant‐based and Fish‐meal‐based Diets
Browdy, C. L., Seaborn, G. T., Atwood, H. L., et al. (2006) · Journal of the World Aquaculture Society · doi:10.1111/j.1749-7345.2006.00057.x · Cited by 43
Synthesis of cyclopropane fatty acid and its effect on freeze-drying survival of Lactobacillus bulgaricus L2 at different growth conditions
Li, C., Zhao, J., Wang, Y., et al. (2009) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-009-0060-0 · Cited by 43
De novo biosynthesis of arachidonic acid and 5,11,14-eicosatrienoic acid in the cricket Teleogryllus commodus
Jurenka, R. A., Stanley‐Samuelson, D. W., Loher, W., et al. (1988) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(88)90333-5 · Cited by 43
Enhancement of polyunsaturated fatty acid production by cerulenin treatment in polyunsaturated fatty acid-producing bacteria
Morita, N., Nishida, T., Tanaka, M., Yano, Y., & Okuyama, H. (2005) · Biotechnology Letters · doi:10.1007/s10529-005-1532-4 · Cited by 42
Transport and fate of Danube Delta terrestrial organic matter in the Northwest Black Sea mixing zone
Saliot, A., Parrish, C. C., Sadouni, N., Bouloubassi, I., Fillaux, J., & Gustave Cauwet, A. (2002) · Marine Chemistry · doi:10.1016/s0304-4203(02)00067-1 · Cited by 43
Effect of Supplementation with Docosahexaenoic Acid Ethyl Ester and sn-2 Docosahexaenyl Monoacylglyceride on Plasma and Erythrocyte Fatty Acids in Rats
Valenzuela, A., Valenzuela, V., Julio Sanhueza, C., & Nieto, S. (2005) · Annals of Nutrition and Metabolism · doi:10.1159/000084177 · Cited by 43
Effect of hydroxycinnamic acids on lipid oxidation and protein changes as well as water holding capacity in frozen minced horse mackerel white muscle
Medina, I., González, M. J., Iglesias, J., et al. (2008) · Food Chemistry · doi:10.1016/j.foodchem.2008.10.031 · Cited by 43
MALDI-TOF/MS analysis of archaebacterial lipids in lyophilized membranes dry-mixed with 9-aminoacridine
Angelini, R., Babudri, F., Lobasso, S., & Corcelli, A. (2010) · Journal of Lipid Research · doi:10.1194/jlr.d007328 · Cited by 43
Promotion of microalgal biomass production and efficient use of CO2 from flue gas by monoethanolamine
Sun, Z., Zhang, D., Yan, C., Cong, W., & Lu, Y. (2014) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.4367 · Cited by 42
Bottlenecks in erucic acid accumulation in genetically engineered ultrahigh erucic acid C rambe abyssinica
Guan, R., Lager, I., Li, X., et al. (2013) · Plant Biotechnology Journal · doi:10.1111/pbi.12128 · Cited by 43
Biochemical Composition and Lipid Compositional Properties of the Brown Alga Sargassum horneri
Hossain, Z., Kurihara, H., & Takahashi, K. (2003) · Pakistan Journal of Biological Sciences · doi:10.3923/pjbs.2003.1497.1500 · Cited by 43
Tissue–diet discrimination factors and turnover of stable carbon and nitrogen isotopes in white-footed mice (Peromyscus leucopus)
DeMots, R. L., Novak, J. M., Gaines, K. F., Gregor, A. J., Romanek, C. S., & Soluk, D. A. (2010) · Canadian Journal of Zoology · doi:10.1139/z10-063 · Cited by 43
Lipidomic analyses of the mouse brain after antidepressant treatment: evidence for endogenous release of long-chain fatty acids?
Lee, L. H.-W., Shui, G., Farooqui, A. A., Wenk, M. R., Tan, C. H., & Ong, W. (2009) · The International Journal of Neuropsychopharmacology · doi:10.1017/s146114570900995x · Cited by 44
Inhibition of Cholesterol Efflux by 7-Ketocholesterol: Comparison between Cells, Plasma Membrane Vesicles, and Liposomes as Cholesterol Donors
Gaus, K., Dean, R. T., Kritharides, L., et al. (2001) · Biochemistry · doi:10.1021/bi010833h · Cited by 43
Granulosicoccus coccoides sp. nov., isolated from leaves of seagrass (Zostera marina)
Kurilenko, V. V., Christen, R., Zhukova, N. V., et al. (2009) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.013516-0 · Cited by 43
1-O-Alkylglycerols Improve Boar Sperm Motility and Fertility1
Cheminade, C., Gautier, V., Hichami, A., et al. (2002) · Biology of Reproduction · doi:10.1095/biolreprod66.2.421 · Cited by 43
Mechanism of the Chilling-Induced Decrease in Proton Pumping across the Tonoplast of Rice Cells
Kasamo, K., Yamaguchi, M., & Nakamura, Y. (2000) · Plant and Cell Physiology · doi:10.1093/pcp/pcd002 · Cited by 43
The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria
Gunnelius, L., Hakkila, K., Kurkela, J., Wada, H., Tyystjärvi, E., & Tyystjärvi, T. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku084 · Cited by 43
Phosphorylation of CTP Synthetase on Ser36, Ser330, Ser354, and Ser454 Regulates the Levels of CTP and Phosphatidylcholine Synthesis in Saccharomyces cerevisiae
Park, T., O'Brien, D. J., & Carman, G. M. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m301394200 · Cited by 43
Metabolism of VLDL is increased in streptozotocin-induced diabetic rat hearts
Sambandam, N., Abrahani, M. A., Craig, S., Al-Atar, O., Jeon, E., & Rodrigues, B. B. (2000) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.2000.278.6.h1874 · Cited by 43
Linoleic acid kinetics and conversion to arachidonic acid in the pregnant and fetal baboon
Su, H., Corso, T. N., Nathanielsz, P. W., & Brenna, J. T. (1999) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)33492-1 · Cited by 40
Dietary sesame (Sesamum indicum cultivar Linn) oil inhibits iron-induced oxidative stress in rats
Hemalatha, S., Raghunath, M., & Ghafoorunissa (2004) · British Journal Of Nutrition · doi:10.1079/bjn20041239 · Cited by 43
The Sinorhizobium medicae WSM419 lpiA gene is transcriptionally activated by FsrR and required to enhance survival in lethal acid conditions
Reeve, W. G., Bräu, L., Castelli, J. M., et al. (2006) · Microbiology · doi:10.1099/mic.0.28764-0 · Cited by 43
Differential Incorporation of Cholesterol by Sindbis Virus Grown in Mammalian or Insect Cells
Hafer, A., Whittlesey, R. L., Brown, D. T., et al. (2009) · Journal of Virology · doi:10.1128/jvi.00755-09 · Cited by 43
Effect of dietary fatty acid composition on fatty acid profiles of polar and neutral lipid tissue fractions in zebra finches, Taeniopygia guttata
McCue, M. D., Amitai, O., Khozin‐Goldberg, I., et al. (2009) · Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology · doi:10.1016/j.cbpa.2009.06.002 · Cited by 43
Spylidone, a Novel Inhibitor of Lipid Droplet Accumulation in Mouse Macrophages Produced by Phoma sp. FKI-1840
Koyama, N., Nagahiro, T., Yamaguchi, Y., Ohshiro, T., Masuma, R., Tomoda, H., & Ōmura, S. (2005) · The Journal of Antibiotics · doi:10.1038/ja.2005.42 · Cited by 43
Sphingomonas histidinilytica sp. nov., isolated from a hexachlorocyclohexane dump site
Nigam, A., Jit, S., & Lal, R. (2009) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.008995-0 · Cited by 43
Winogradskyella ulvae sp. nov., an epiphyte of a Pacific seaweed, and emended descriptions of the genus Winogradskyella and Winogradskyella thalassocola , Winogradskyella echinorum , Winogradskyella exilis and Winogradskyella eximia
Nedashkovskaya, O. I., Kukhlevskiy, A. D., & Zhukova, N. V. (2011) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.032219-0 · Cited by 43
Essential fatty acid deficiency associated with the use of a medium-chain-triglyceride infant formula in pediatric hepatobiliary disease
Pettei, M. J., Daftary, S. S., & Levine, J. J. (1991) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/53.5.1217 · Cited by 43
Development of the pulmonary surfactant system in two oviparous vertebrates
Johnston, S. D., Orgeig, S., Lopatko, O. V., et al. (2000) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.2000.278.2.r486 · Cited by 43
Fatty acid composition of human milk in atopic Danish mothers
Lauritzen, L., Halkjær, L. B., Mikkelsen, T. B., et al. (2006) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/84.1.190 · Cited by 43
Platelet lipid(s) bound to albumin increases endothelial electrical resistance: mimicked by LPA
Minnear, F. L., Patil, S., Bell, D. R., et al. (2001) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.2001.281.6.l1337 · Cited by 43
Lipoprotein-cholesterol responses in healthy infants fed defined diets from ages 1 to 12 months: comparison of diets predominant in oleic acid versus linoleic acid, with parallel observations in infants fed a human milk-based diet.
Mize, C. E., Uauy, R. D., Kramer, R. E., et al. (1995) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)41126-5 · Cited by 43
Altered post-mortem metabolism identified in very fast chilled lamb M. longissimus thoracis et lumborum using metabolomic analysis
Warner, R. D., Jacob, R. H., Rosenvold, K., et al. (2015) · Meat Science · doi:10.1016/j.meatsci.2015.06.006 · Cited by 43
Effect of vitamin K depletion and restoration on sphingolipid metabolism in Bacteroides melaninogenicus
Lev, M., & Milford, A. F. (1972) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)39399-8 · Cited by 43
Diet immersion time: effects on growth, survival and feeding behaviour of juvenile southern rock lobster, Jasus edwardsii
Tolomei, A., Crear, B. J., & Johnston, D. (2003) · Aquaculture · doi:10.1016/s0044-8486(02)00507-0 · Cited by 43
Effect of alcohols and temperature on the hopanoid content of Zymomonas mobilis
Schmidt, A., Bringer‐Meyer, S., Poralla, K., & Sahm, H. (1986) · Applied Microbiology and Biotechnology · doi:10.1007/bf00252509 · Cited by 43
Effect of variable nutrient supply on fatty acid composition of phytoplankton grown in an enclosed experimental ecosystem
Mayzaud, P., Claustre, H., & Augier, P. (1990) · Marine Ecology Progress Series · doi:10.3354/meps060123 · Cited by 43
The deficient degradation of synthetic 2‐ and 3‐methyl‐branched fatty acids in fibroblasts from patients with peroxisomal disorders
Veldhoven, P. P. V., Huang, S., Eyssen, H., et al. (1993) · Journal of Inherited Metabolic Disease · doi:10.1007/bf00710285 · Cited by 43
Metabolism of n-pentane by ethanol-inducible cytochrome P-450 in liver microsomes and reconstituted membranes
Terelius, Y., & Ingelman‐Sundberg, M. (1986) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1986.tb10447.x · Cited by 43
Development of a supercritical fluid extraction method for determination of lipid classes and total fat in meats and its comparison with conventional methods
Berg, H., Mågård, M., Johansson, G., et al. (1997) · Journal of Chromatography A · doi:10.1016/s0021-9673(97)00686-9 · Cited by 43
The essential fatty acid requirement of milkfish (Chanos chanos Forsskal)
Borlongan, I. G. (1992) · Fish Physiology and Biochemistry · doi:10.1007/bf02274221 · Cited by 43
Storage and depletion of lipid components in the pyloric caeca and ovaries of the seastarAsterias rubens during its annual reproductive cycle
Oudejans, R. C. H. M., & van der Sluis, I. (1979) · Marine Biology · doi:10.1007/bf00952432 · Cited by 43
Shift in sphingolipid metabolism leads to an accumulation of ceramide in senescence
Venable, M. E., Webb-Froehlich, L. M., Sloan, E. F., & Thomley, J. E. (2006) · Mechanisms of Ageing and Development · doi:10.1016/j.mad.2006.01.003 · Cited by 43
Comparison of Methods to Measure the Oil Contents in Oilseeds.
Barthet, V. J., Chornick, T., & Daun, J. K. (2002) · Journal of Oleo Science · doi:10.5650/jos.51.589 · Cited by 43
Elevated levels of cellular and extracellular phospholipases from pathogenic Naegleria fowleri
Hysmith, R. M., & Franson, R. C. (1982) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(82)90005-4 · Cited by 43
Abnormal Phospholipid Composition Impairs HDL Biogenesis and Maturation in Mice Lacking Abca1
Francone, O. L., Subbaiah, P. V., van Tol, A., Royer, L. J., & Haghpassand, M. (2003) · Biochemistry · doi:10.1021/bi034540v · Cited by 43
Effect of ethanol on platelet phospholipase A2
Stubbs, C. D., & Rubin, R. (1992) · Lipids · doi:10.1007/bf02536471 · Cited by 43
A 13C‐NMR study of lipid alterations during fish canning: Effect of filling medium
Medina, I., Sacchi, R., & Aubourg, S. P. (1995) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.2740690407 · Cited by 43
Degradation of dipalmitoyl phosphatidylcholine by isolated rat granular pneumocytes and reutilization for surfactant synthesis.
Chander, A., Reicherter, J., & Fisher, A. B. (1987) · Journal of Clinical Investigation · doi:10.1172/jci112929 · Cited by 43
Lipophorin from Rhodnius prolixus: Purification and partial characterization
Gondim, K. C., de Oliveira, P. L., Coelho, H. S. L., et al. (1989) · Insect Biochemistry · doi:10.1016/0020-1790(89)90086-3 · Cited by 43
Temperature acclimation of Trypanosoma cruzi epimastigote and metacyclic trypomastigote lipids
Florin‐Christensen, M., Florin‐Christensen, J., de Isola, E. D., et al. (1997) · Molecular and Biochemical Parasitology · doi:10.1016/s0166-6851(97)00056-x · Cited by 43
Characterization of a G Protein-activated Phosphoinositide 3-Kinase in Vascular Smooth Muscle Cell Nuclei
Bacqueville, D., Déléris, P., Mendre, C., Pieraggi, M. T., Chap, H., Guillon, G., Perret, B., & Breton, M. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m011572200 · Cited by 43
Increase of serum phosphatidylcholine hydroperoxide dependent on glycemic control in type 2 diabetic patients
Nagashima, T., Oikawa, S., Hirayama, Y., Tokita, Y., Sekikawa, A., Ishigaki, Y., Yamada, R., & Miyazawa, T. (2002) · Diabetes Research and Clinical Practice · doi:10.1016/s0168-8227(01)00353-9 · Cited by 43
Relation of feeding, growth, and metabolism to age in the larval, female house cricket
Woodring, J. P., Roe, R. M., & Clifford, C. W. (1977) · Journal of Insect Physiology · doi:10.1016/0022-1910(77)90031-2 · Cited by 43
Fatty acids in decomposing mangrove leaves: microbial activity, decay and nutritional quality
Mfilinge, P. L., Meziane, T., Bachok, Z., et al. (2003) · Marine Ecology Progress Series · doi:10.3354/meps265097 · Cited by 43
Photosensitized killing of cultured fibroblasts from patients with peroxisomal disorders due to pyrene fatty acid-mediated ultraviolet damage.
Höefler, G., Paschke, E., Hoefler, S., Moser, A. B., & Moser, H. W. (1991) · Journal of Clinical Investigation · doi:10.1172/jci115509 · Cited by 43
A comparison of four methods for measuring respiration in organic material
Cleve, K. V., Coyne, P., Goodwin, E., et al. (1979) · Soil Biology and Biochemistry · doi:10.1016/0038-0717(79)90068-3 · Cited by 43
Regulators of sperm function: A role for diacylglycerol in human sperm acrosomal exocytosis
O'Toole, C. M. B., Roldán, E. R. S., Hampton, P., et al. (1996) · Molecular Human Reproduction · doi:10.1093/molehr/2.5.317 · Cited by 43
Physiological Effects of Whole Cottonseed in the Diet of Lactating Dairy Cows
Hawkins, G. E., Cummins, K. A., Silverio, M., et al. (1985) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(85)81144-9 · Cited by 43
Phospholipid patterns of erythrocytes in schizophrenia: relationships to symptomatology
Ponizovsky, A. M., Modai, I., Nechamkin, Y., Barshtein, G., Ritsner, M., Yedgar, S., Lecht, S., & Bergelson, L. D. (2001) · Schizophrenia Research · doi:10.1016/s0920-9964(00)00189-4 · Cited by 42
Effects of IGF-I on 1,25-dihydroxyvitamin D3 synthesis by human placenta in culture
Halhalí, A. (1999) · Molecular Human Reproduction · doi:10.1093/molehr/5.8.771 · Cited by 43
23S,25-dihydroxyvitamin D3 as a circulating metabolite of vitamin D3. Its role in 25-hydroxyvitamin D3-26,23-lactone biosynthesis.
Napoli, J. L., Pramanik, B. C., Partridge, J. J., et al. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)34119-x · Cited by 43
Lipids in tissues of the eye
BROEKHUYSE, R. M. (1972) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(72)90143-9 · Cited by 43
Phospholipid metabolism of herpesvirus-infected and uninfected rabbit kidney cells
Ben‐Porat, T., & Kaplan, A. S. (1971) · Virology · doi:10.1016/0042-6822(71)90132-2 · Cited by 43
Effects of dietary vitamin E on the biosynthesis of 5-lipoxygenase products by rat polymorphonuclear leukocytes (PMNL)
Chan, A. C., Tran, K. T., Pyke, D. D., et al. (1989) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(89)90047-7 · Cited by 43
Abnormal platelet membrane composition in Alzheimer's-type dementia
Cohen, B. M., Zubenko, G. S., & Babb, S. M. (1987) · Life Sciences · doi:10.1016/0024-3205(87)90760-0 · Cited by 43
Identification of detergent-resistant plasma membrane microdomains in dictyostelium: enrichment of signal transduction proteins.
Xiao, Z., & Devreotes, P. N. (1997) · Molecular Biology of the Cell · doi:10.1091/mbc.8.5.855 · Cited by 43
Hydroxyarchaetidylserine and hydroxyarchaetidyl-myo-inositol in Methanosarcina barkeri: polar lipids with a new ether core portion
Nishihara, M., & Koga, Y. (1991) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(91)90196-o · Cited by 43
Cumulative stress-induced mortality of gizzard shad in a southeastern U.S. reservoir
Adams, S. M., Breck, J. E., & McLean, R. B. (1985) · Environmental Biology of Fishes · doi:10.1007/bf00002578 · Cited by 43
Involvement of Phosphatidylinositol 3′‐Kinase in Stem‐Cell‐Factor‐Induced Phospholipase D Activation and Arachidonic Acid Release
Kozawa, O., Blume‐Jensen, P., Heldin, C., & Rönnstrand, L. (1997) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1997.00149.x · Cited by 43
A Chinese hamster ovary cell mutant defective in the non-endocytic uptake of fluorescent analogs of phosphatidylserine: isolation using a cytosol acidification protocol.
Hanada, K., & Pagano, R. E. (1995) · The Journal of Cell Biology · doi:10.1083/jcb.128.5.793 · Cited by 43
COMPARATIVE EFFECTS OF FOLIAR‐OR SOIL‐APPLIED NITRATE ON VESICULAR‐ARBUSCULAR MYCORRHIZAL INFECTION IN MAIZE
Azcón, R., Gómez-Ortega, M., & Barea, J. M. (1982) · New Phytologist · doi:10.1111/j.1469-8137.1982.tb03414.x · Cited by 43
Oxygen consumption and ammonia excretion of the brown tiger prawn Penaeus esculentus fed diets of varying protein content
Hewitt, D. R., & Irving, M. G. (1990) · Comparative Biochemistry and Physiology Part A Physiology · doi:10.1016/0300-9629(90)90098-d · Cited by 43
Fat body heterogeneity during development of Heliothis zea
Haunerland, N. H., Nair, K. K., & Bowers, W. S. (1990) · Insect Biochemistry · doi:10.1016/0020-1790(90)90101-y · Cited by 43
Purification and Characterization from Rat Kidney Membranes of a Novel Platelet-activating Factor (PAF)-dependent Transacetylase That Catalyzes the Hydrolysis of PAF, Formation of PAF Analogs, and C2-ceramide
Karasawa, K., Qiu, X., & Lee, T. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.13.8655 · Cited by 43
Biosynthetic studies of the polar lipids of Halobacterium cutirubrum. Formation of isoprenyl ether intermediates
Moldoveanu, N., & Kates, M. (1988) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(88)90062-8 · Cited by 44
In Vitro Metabolism of the Vitamin D Analog, 22-Oxacalcitriol, Using Cultured Osteosarcoma, Hepatoma, and Keratinocyte Cell Lines
増田, 園., Byford, V., Kremer, R. B., et al. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.15.8700 · Cited by 43
Lipid metabolism in the parasitic and free-living spirochetes Treponema pallidum (Reiter) and Treponema zuelzerae
Meyer, H., & Meyer, F. (1971) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(71)90257-8 · Cited by 43
Oxidation of Dilinoleoyl Phosphatidylcholine by Lipoxygenase 1 from Soybeans
Pérez‐Gilabert, M., Veldink, G. A., & Vliegenthart, J. F. G. (1998) · Archives of Biochemistry and Biophysics · doi:10.1006/abbi.1998.0673 · Cited by 43
Aliphatic hydrocarbon contents of various members of the family micrococcaceae
Tornabene, T. G., Morrison, S. J., & Kloos, W. E. (1970) · Lipids · doi:10.1007/bf02531125 · Cited by 43

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks