Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 14,801–14,900 of 48,846

Negative impact of tissue inhibitor of metalloproteinase-3 null mutation on lung structure and function in response to sepsis
Martin, E. L., Moyer, B. Z., Pape, M. C., STARCHER, B. C., Leco, K. J., & Veldhuizen, R. A. W. (2003) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00141.2003 · Cited by 46
Stereospecific chemoattraction of lymphoblastic cells by gradients of lysophosphatidylcholine.
Hoffman, R. D., Kligerman, M., Sundt, T. M., Anderson, N. D., & Shin, H. S. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.10.3285 · Cited by 44
Mutants of Escherichia coli defective in membrane phospholipid synthesis. Effect of cessation of net phospholipid synthesis on cytoplasmic and outer membranes
McIntyre, T. M., & Bell, R. (1975) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)40692-3 · Cited by 44
Microsomal Triacylglycerol Transfer Protein Prevents Presecretory Degradation of Apolipoprotein B‐100
Benoist, F., Nicodème, E., & Grand‐Perret, T. (1996) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1996.0713h.x · Cited by 44
Carbohydrate composition of central nervous system synapses
Churchill, L., Cotman, C. W., Banker, G. A., Kelly, P., & Shannon, L. M. (1976) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(76)90076-6 · Cited by 45
Constitution of the cell envelope of Haemophilus influenzae in relation to competence for genetic transformation
Zoon, K. C., & Scocca, J. J. (1975) · Journal of Bacteriology · doi:10.1128/jb.123.2.666-677.1975 · Cited by 44
Sulfolipid synthesis from 35SO42− and [1-14C]acetate in isolated intact spinach chloroplasts
Joyard, J., Blée, E., & Douce, R. (1986) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(86)90269-9 · Cited by 44
Retinoic acid modulation of 1,25(OH)2 vitamin D3 receptors and bioresponse in bone cells: Species differences between rat and mouse
Chen, T. L., & Feldman, D. I. (1985) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(85)90990-8 · Cited by 44
Induction of microsomal stearoyl-CoA desaturation by the administration of various peroxisome proliferators
Yoichi, K., HANIOKA, N., Matsumura, M., et al. (1983) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(83)90121-2 · Cited by 44
Detection of nanogram quantities of vitamin D by gas-liquid chromatography
Avioli, L. V., & Lee, S. W. (1966) · Analytical Biochemistry · doi:10.1016/0003-2697(66)90146-1 · Cited by 44
Effect of high fat corn oil, olive oil and fish oil on phospholipid fatty acid composition in male F344 rats
Rao, C. V., Zang, E. A., & Reddy, B. S. (1993) · Lipids · doi:10.1007/bf02535943 · Cited by 43
Phosphatidic Acid Synthesis in Castor Bean Endosperm
Vick, B. A., & Beevers, H. (1977) · PLANT PHYSIOLOGY · doi:10.1104/pp.59.3.459 · Cited by 44
Phosphatidic Acid and Glyceride Synthesis by Particles from Spinach Leaves
Cheniae, G. M. (1965) · PLANT PHYSIOLOGY · doi:10.1104/pp.40.2.235 · Cited by 44
Kinetics of individual polychlorinated biphenyl (PCB) components in juvenile sole (Solea solea) in relation to their concentrations in food and to lipid metabolism
Boon, J. P., Oudejans, R. C. H. M., & Duinker, J. C. (1984) · Comparative Biochemistry and Physiology Part C Comparative Pharmacology · doi:10.1016/0742-8413(84)90175-0 · Cited by 44
The solubility of cholesterol and its exchange between membranes
Bruckdorfer, K. R., & Sherry, M. K. (1984) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(84)90022-1 · Cited by 44
INFLUENCE OF SEX AND POSTMORTEM AGING ON INTRAMUSCULAR AND SUBCUTANEOUS BOVINE LIPIDS
HOOD, R. L., & Allen, E. M. (1971) · Journal of Food Science · doi:10.1111/j.1365-2621.1971.tb03306.x · Cited by 44
Changes in phospholipids of ram spermatozoa during migration through the epididymis and possible origin of prostaglandin F2α in testicular and epididymal fluid
Poulos, A., Brown-Woodman, P. D. C., White, I. G., et al. (1975) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(75)90057-0 · Cited by 44
Semisynthesis and purification of homogeneous plasmenylcholine molecular species
Han, X., Zupan, L. A., Hazen, S. L., & Gross, R. W. (1992) · Analytical Biochemistry · doi:10.1016/0003-2697(92)90286-g · Cited by 44
Studies on enzymes related to diacylglycerol production in activated platelets II. Subcellular distribution, enzymatic properties and positional specificity of diacylglycerol- and monoacylglycerol-lipases
Mauco, G., Fauvel, J., Chap, H., et al. (1984) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(84)90345-x · Cited by 44
Copper and cell‐oxidized low‐density lipoprotein induces activator protein 1 in fibroblasts, endothelial and smooth muscle cells
Mazière, C., Djavaheri‐Mergny, M., Frey-Fressart, V., Delattre, J., & Mazière, J. (1997) · FEBS Letters · doi:10.1016/s0014-5793(97)00545-0 · Cited by 44
Involvement of cholecalciferol metabolism in birds in the adaptation of calcium absorption to the needs during reproduction
Bar, A., Cohen, A., Edelstein, S., et al. (1978) · Comparative Biochemistry and Physiology Part B Comparative Biochemistry · doi:10.1016/0305-0491(78)90254-7 · Cited by 44
Phosphatidylcholine Hydrolysis and c- myc Expression Are in Collaborating Mitogenic Pathways Activated by Colony-Stimulating Factor 1
Xu, X., Tessner, T. G., Rock, C. O., & Jackowski, S. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.3.1522-1533.1993 · Cited by 44
Molecular Stratigraphic Study of Sediments from Holes 658A and 660A, Leg 108
Poynter, J., Farrimond, P., Brassell, S. C., et al. (1989) · doi:10.2973/odp.proc.sr.108.161.1989 · Cited by 44
Studies on the metabolism of calciferol XII
Friedlander, E. J., & Norman, A. W. (1975) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(75)90171-x · Cited by 44
Metabolism of 1-O-alkyl-2-acetyl-sn-glycerol by washed rabbit platelets: Formation of platelet activating factor
Satouchi, K., Oda, M., Saito, K., et al. (1984) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(84)90355-2 · Cited by 44
The chemical composition of trypanosomes. III. Antigenic constituents of Brucei trypanosomes
Williamson, J. S., & Brown, K. N. (1964) · Experimental Parasitology · doi:10.1016/0014-4894(64)90006-2 · Cited by 44
Cellular triglyceride as a source of fatty acid for lecithin synthesis during phagocytosis
Elsbach, P., & Farrow, S. (1969) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(69)90208-2 · Cited by 44
Regional differences in arterial low density lipoprotein metabolism in surgically postmenopausal cynomolgus monkeys. Effects of estrogen and progesterone replacement therapy.
Wagner, J. D., Clair, R. W. S., Schwenke, D. C., et al. (1992) · Arteriosclerosis and Thrombosis A Journal of Vascular Biology · doi:10.1161/01.atv.12.6.717 · Cited by 44
Phospholipase C-mediated hydrolysis of phosphatidylcholine is activated by muscarinic agonists
Díaz-Meco, M. T., Larrodera, P., López-Barahona, M., et al. (1989) · Biochemical Journal · doi:10.1042/bj2630115 · Cited by 44
The hepatic WASH complex is required for efficient plasma LDL and HDL cholesterol clearance
Wijers, M., Zanoni, P., Liv, N., Vos, D. Y., Jäckstein, M. Y., Smit, M., Wilbrink, S., Wolters, J. C., van der Veen, Y. T., Huijkman, N., et al. (2019) · JCI Insight · doi:10.1172/jci.insight.126462 · Cited by 44
Pyruvate kinase variant of fission yeast tunes carbon metabolism, cell regulation, growth and stress resistance
Kamrad, S., Grossbach, J., Rodríguez‐López, M., Mülleder, M., Townsend, S., Cappelletti, V., Stojanovski, G., Correia‐Melo, C., Picotti, P., Beyer, A., et al. (2020) · Molecular Systems Biology · doi:10.15252/msb.20199270 · Cited by 40
“The samples were extracted as described (Bligh & Dyer, 1959).”
Method
A controlled-release mitochondrial protonophore reverses hypertriglyceridemia, nonalcoholic steatohepatitis, and diabetes in lipodystrophic mice
Abulizi, A., Perry, R. J., Camporez, J. P. G., Jurczak, M. J., Petersen, K. F., Aspichueta, P., & Shulman, G. I. (2017) · The FASEB Journal · doi:10.1096/fj.201700001r · Cited by 41
The estrogen-responsive Agr2 gene regulates mammary epithelial proliferation and facilitates lobuloalveolar development
Verma, S., Salmans, M., Geyfman, M., Wang, H., Yu, Z., Lu, Z., Zhao, F. R., Lipkin, S. M., & D, B. A. M. (2012) · Developmental Biology · doi:10.1016/j.ydbio.2012.06.030 · Cited by 44
Multiple effects of cold exposure on livers of male mice
Grefhorst, A., van den Beukel, J. C., Dijk, W., Steenbergen, J., Voortman, G. J., Leeuwenburgh, S., Visser, T. J., Kersten, S., Friesema, E. C. H., Themmen, A. P. N., et al. (2018) · Journal of Endocrinology · doi:10.1530/joe-18-0076 · Cited by 43
Recent advances in the mass spectrometric analysis of glycosphingolipidome – A review
Barrientos, R. C., & Zhang, Q. (2020) · Analytica Chimica Acta · doi:10.1016/j.aca.2020.05.051 · Cited by 43
“In lipidomics studies, total lipids are typically extracted using CHCl3/CH3OH (2:1) in the Folch method [107], CHCl3/CH3OH (1:2) in the Bligh and Dyer method [108], or 1butanol/CH3OH (3:1) in the butanol-methanol (BUME) method [109].”
Method
Mitochondrial sphingosine-1-phosphate lyase is essential for phosphatidylethanolamine synthesis and survival of Trypanosoma brucei
Nejad, L. D., Stumpe, M., Rauch, M., Hemphill, A. F., Schneiter, R., Bütikofer, P., & Serricchio, M. (2020) · Scientific Reports · doi:10.1038/s41598-020-65248-x · Cited by 43
Different Biochemical Compositions of Particulate Organic Matter Driven by Major Phytoplankton Communities in the Northwestern Ross Sea
Jo, N., La, H. S., Kim, J., Kim, K., Kim, B. K., Kim, M. J., Son, W., & Lee, S. H. (2021) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.623600 · Cited by 41
“The total LIP was extracted by chloroform and methanol (1:2, v:v) according to the modified method of Bligh and Dyer (1959) and Marsh and Weinstein (1966).”
Method
Evaluation and optimisation of direct transesterification methods for the assessment of lipid accumulation in oleaginous filamentous fungi
Langseter, A. M., Dzurendová, S., Shapaval, V., Köhler, A., Ekeberg, D., & Zimmermann, B. (2021) · Microbial Cell Factories · doi:10.1186/s12934-021-01542-1 · Cited by 46
“Compared to the standard two-step extractiontransesterification methods, such as Folch and Bligh and Dyer methods [8, 9], direct transesterification often results in higher FAMEs yields [17, 18, 21].”
Method
Elucidating the bioremediation mechanism of Scenedesmus sp. IITRIND2 under cadmium stress
Tripathi, S., Arora, N., Pruthi, V., & Poluri, K. M. (2021) · Chemosphere · doi:10.1016/j.chemosphere.2021.131196 · Cited by 45
UVB exposure stimulates production of vitamin D3 in selected microalgae
Ljubić, A., Thulesen, E. T., Jacobsen, C., & Jakobsen, J. (2021) · Algal Research · doi:10.1016/j.algal.2021.102472 · Cited by 43
Porphyromonas gingivalis induces entero-hepatic metabolic derangements with alteration of gut microbiota in a type 2 diabetes mouse model
Kashiwagi, Y., Aburaya, S., Sugiyama, N., Narukawa, Y., Sakamoto, Y., Takahashi, M., Uemura, H., Yamashita, R., Tominaga, S., Hayashi, S., et al. (2021) · Scientific Reports · doi:10.1038/s41598-021-97868-2 · Cited by 50
Raman microscopy-based quantification of the physical properties of intracellular lipids
Uematsu, M., & Shimizu, T. (2021) · Communications Biology · doi:10.1038/s42003-021-02679-w · Cited by 43
Marine and terrestrial nitrifying bacteria are sources of diverse bacteriohopanepolyols
Elling, F. J., Evans, T. W., Nathan, V., Hemingway, J., Kharbush, J. J., Bayer, B., Spieck, E., Husain, F., Summons, R. E., & Pearson, A. (2022) · Geobiology · doi:10.1111/gbi.12484 · Cited by 49
Studies on trimethylsilyl derivatives of 1-alkyl-2-acylglycerols by gas-liquid chromatography mass spectrometry
Satouchi, K., & Saito, K. (1976) · Journal of Mass Spectrometry · doi:10.1002/bms.1200030306 · Cited by 43
Scenedesmus sp. strain SD07 cultivation in municipal wastewater for pollutant removal and production of lipid and exopolysaccharides
Silambarasan, S., Logeswari, P., Sivaramakrishnan, R., Incharoensakdi, A., Kamaraj, B., & Cornejo, P. (2022) · Environmental Research · doi:10.1016/j.envres.2022.115051 · Cited by 48
Analysis of milk with liquid chromatography–mass spectrometry: a review
Imperiale, S., Morozova, K., Ferrentino, G., & Scampicchio, M. (2023) · European Food Research and Technology · doi:10.1007/s00217-022-04197-3 · Cited by 48
“The most common extraction techniques include methods by Folch [36], and Bligh–Dyer [37].”
Method
Portable near-infrared (NIR) spectrometer and chemometrics for rapid identification of butter cheese adulteration
Medeiros, M. L. D. S., Lima, A. F., Gonçalves, M. C., et al. (2023) · Food Chemistry · doi:10.1016/j.foodchem.2023.136461 · Cited by 43
Three diacylglycerol acyltransferases contribute to oil biosynthesis and normal growth in Yarrowia lipolytica
Zhang, H., Damude, H. G., & Yadav, N. S. (2011) · Yeast · doi:10.1002/yea.1914 · Cited by 43
Oral supplementation with fish oil reduces dryness and pruritus in the acetone-induced dry skin rat model
Barcelos, R. C. S., de Mello-Sampayo, C., Antoniazzi, C. T. D. D., Segat, H. J., Silva, H., Veit, J. C., Piccolo, J., Emanuelli, T., Bürger, M. E., Lima, B. S., et al. (2015) · Journal of Dermatological Science · doi:10.1016/j.jdermsci.2015.06.015 · Cited by 46
A comparative study of organic- versus conventional farmed Atlantic salmon. I. Pigment and lipid content and composition, and carotenoid stability in ice-stored fillets
Lerfall, J., Bendiksen, E. Å., Olsen, J. V., et al. (2015) · Aquaculture · doi:10.1016/j.aquaculture.2015.09.013 · Cited by 43
The Antimicrobial and Antioxidant Effects of C itrus aurantium L. Flowers ( B ahar N arang) Extract in Traditional Yoghurt Stew during Refrigerated Storage
Hashemi, S. M. B., Amininezhad, R., Shirzadinezhad, E., et al. (2015) · Journal of Food Safety · doi:10.1111/jfs.12222 · Cited by 43
Antibiotic Effects on Microbial Community Characteristics in Soils under Conservation Management Practices
Unger, I. M., Goyne, K. W., Kennedy, A. C., et al. (2012) · Soil Science Society of America Journal · doi:10.2136/sssaj2012.0099 · Cited by 43
Genetic manipulation, a feasible tool to enhance unique characteristic of Chlorella vulgaris as a feedstock for biodiesel production
Talebi, A. F., Tohidfar, M., Tabatabaei, M., Bagheri, A., Mohsenpour, M., & Mohtashami, S. K. (2013) · Molecular Biology Reports · doi:10.1007/s11033-013-2532-4 · Cited by 46
Bioconcentration of triclosan and methyl-triclosan in marine mussels (Mytilus galloprovincialis) under laboratory conditions and in metropolitan waters of Gulf St Vincent, South Australia
Kookana, R. S., Shareef, A. A., Fernandes, M. B., Hoare, S. L., Gaylard, S. G., & Kumar, A. (2013) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2013.07.030 · Cited by 42
Microplate assay for quantitation of neutral lipids in extracts from microalgae
Higgins, B. T., Thornton-Dunwoody, A., Labavitch, J. M., & VanderGheynst, J. S. (2014) · Analytical Biochemistry · doi:10.1016/j.ab.2014.07.020 · Cited by 45
Multi-organ Abnormalities and mTORC1 Activation in Zebrafish Model of Multiple Acyl-CoA Dehydrogenase Deficiency
Kim, S.-H., Scott, S. A., Bennett, M. J., et al. (2013) · PLoS Genetics · doi:10.1371/journal.pgen.1003563 · Cited by 43
Degradation of chlorpyrifos using different biostimulants/biofertilizers: Effects on soil biochemical properties and microbial community
Tejada, M., Rodríguez-Morgado, B., Gómez, I., et al. (2014) · Applied Soil Ecology · doi:10.1016/j.apsoil.2014.07.007 · Cited by 43
Biochemical characterization of a chloroplast localized fatty acid reductase from Arabidopsis thaliana
Doan, T. T. P., Domergue, F., Fournier, A. E., et al. (2011) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2011.10.019 · Cited by 43
Deciphering mutant ELOVL4 activity in autosomal-dominant Stargardt macular dystrophy
Logan, S., Agbaga, M., Chan, M. D., Kabir, N., Mandal, N. A., Brush, R. S., & Anderson, R. E. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1217251110 · Cited by 44
High-Throughput Phospholipidic Fingerprinting by Online Desorption of Dried Spots and Quadrupole-Linear Ion Trap Mass Spectrometry: Evaluation of Atherosclerosis Biomarkers in Mouse Plasma
Thomas, A., Déglon, J., Lenglet, S., et al. (2010) · Analytical Chemistry · doi:10.1021/ac101421b · Cited by 43
Lipid production by microalgaeChlorella pyrenoidosacultivated in palm oil mill effluent (POME) using hybrid photo bioreactor (HPBR)
Kamyab, H., Din, M. F. M., Lee, C. T., Keyvanfar, A., Shafaghat, A., Majid, M. Z. A., Ponraj, M., & Yun, T. X. (2014) · Desalination and Water Treatment · doi:10.1080/19443994.2014.957943 · Cited by 45
Carotenoids from Foods of Plant, Animal and Marine Origin: An Efficient HPLC-DAD Separation Method
Strati, I. F., Sinanoglou, V. J., Κόρα, Λ., et al. (2012) · Foods · doi:10.3390/foods1010052 · Cited by 43
In vivo hyperoxic preconditioning protects against rat-heart ischemia/reperfusion injury by inhibiting mitochondrial permeability transition pore opening and cytochrome c release
Petrosillo, G., Venosa, N. D., Moro, N., Colantuono, G., Paradies, V., Tiravanti, E., Federici, A., Ruggiero, F. M., & Paradies, G. (2010) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2010.11.030 · Cited by 42
Alteration of Wax Ester Content and Composition in Euglena gracilis with Gene Silencing of 3‐ketoacyl‐CoA Thiolase Isozymes
Nakazawa, M., Andoh, H., Koyama, K., et al. (2015) · Lipids · doi:10.1007/s11745-015-4010-3 · Cited by 42
Ethanol tolerance and membrane fatty acid adaptation in multiple and null mutants of
Heipieper, H. J., Isken, S., & Saliola, M. (2000) · Research in Microbiology · doi:10.1016/s0923-2508(00)01143-8 · Cited by 43
The effectiveness of arbuscular-mycorrhizal fungi and Aspergillus niger or Phanerochaete chrysosporium treated organic amendments from olive residues upon plant growth in a semi-arid degraded soil
Medina, A., Roldán, A., & Azcón, R. (2010) · Journal of Environmental Management · doi:10.1016/j.jenvman.2010.07.008 · Cited by 43
Effect of 2,6-di-O-methyl-α-cyclodextrin on hemolysis and morphological change in rabbit's red blood cells
Motoyama, K., Arima, H., Toyodome, H., et al. (2006) · European Journal of Pharmaceutical Sciences · doi:10.1016/j.ejps.2006.06.002 · Cited by 43
Choline concentrations are lower in postnatal plasma of preterm infants than in cord plasma
Bernhard, W., Raith, M., Kunze, R., et al. (2014) · European Journal of Nutrition · doi:10.1007/s00394-014-0751-7 · Cited by 43
Enhancement of cell growth rate by light irradiation in the cultivation of Rhodotorula glutinis
Yen, H., & Zhang, Z. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2011.06.062 · Cited by 43
Evaluation of maintenance energy requirements in the cultivation of Scenedesmus obliquus: effect of light intensity and regime
Sforza, E., Urbani, S., & Bertucco, A. (2014) · Journal of Applied Phycology · doi:10.1007/s10811-014-0460-x · Cited by 43
Chemical characterization and use of artichoke parts for protection from oxidative stress in canola oil
Claus, T., Maruyama, S. A., Palombini, S. V., et al. (2014) · LWT · doi:10.1016/j.lwt.2014.12.050 · Cited by 43
Differential degradation of intact polar and core glycerol dialkyl glycerol tetraether lipids upon post-depositional oxidation
Lengger, S. K., Kraaij, M., Tjallingii, R., Baas, M., Stuut, J.-B. W., Hopmans, E. C., Damsté, J. S. S., & Stefan Schouten, A. (2013) · Organic Geochemistry · doi:10.1016/j.orggeochem.2013.10.004 · Cited by 43
Treatment of cattle-slaughterhouse wastewater and the reuse of sludge for biodiesel production by microalgal heterotrophic bioreactors
Maroneze, M. M., Barin, J. S., de Menezes, C. R., Queiroz, M. I., Zepka, L. Q., & Lopes, E. J. (2014) · Scientia Agricola · doi:10.1590/0103-9016-2014-0092 · Cited by 43
Metabolomics using GC–TOF–MS followed by subsequent GC–FID and HILIC–MS/MS analysis revealed significantly altered fatty acid and phospholipid species profiles in plasma of smokers
Müller, D., Degen, C., Scherer, G., Jahreis, G., Nießner, R., & Scherer, M. (2014) · Journal of Chromatography B · doi:10.1016/j.jchromb.2014.02.044 · Cited by 45
Phosphatidylethanolamine Synthesis Is Required for Optimal Virulence of Brucella abortus
Bukata, L., Altabe, S., de Mendoza, D., et al. (2008) · Journal of Bacteriology · doi:10.1128/jb.01069-08 · Cited by 43
Identification of Phosphomethylethanolamine N-Methyltransferase from Arabidopsis and Its Role in Choline and Phospholipid Metabolism
BeGora, M. D., MacLeod, M. J. R., McCarry, B. E., et al. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.112151 · Cited by 43
Energy deficit does not affect immune responses of experimentally infected pacu (Piaractus mesopotamicus)
Gimbo, R. Y., Fávero, G. C., Franco‐Montoya, L. N., et al. (2015) · Fish & Shellfish Immunology · doi:10.1016/j.fsi.2015.01.005 · Cited by 43
Interchangeable Domains in the Kdo Transferases of Escherichia coli and Haemophilus influenzae
Chung, H. S., & Raetz, C. R. H. (2010) · Biochemistry · doi:10.1021/bi100343e · Cited by 43
Strategies for the analysis of chlorinated lipids in biological systems
Wacker, B. K., Albert, C. J., Ford, B. A., & Ford, D. A. (2012) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2012.06.013 · Cited by 42
Influence of lifelong dietary fats on the brain fatty acids and amphetamine-induced behavioral responses in adult rat
Trevizol, F., Roversi, K., Dias, V. T., Roversi, K., Pase, C. S., Barcelos, R. C. S., Benvegnú, D. M., Kuhn, F. T., Dolci, G. S., Ross, D. H., et al. (2013) · Progress in Neuro-Psychopharmacology and Biological Psychiatry · doi:10.1016/j.pnpbp.2013.06.007 · Cited by 42
Glycosidic Bond Cleavage is Not Required for Phytosteryl Glycoside‐Induced Reduction of Cholesterol Absorption in Mice
Lin, X., Ma, L., Moreau, R. A., & Ostlund, R. E. (2011) · Lipids · doi:10.1007/s11745-011-3560-2 · Cited by 43
Lipid accumulation by Rhodococcus rhodochrous grown on glucose
Shields‐Menard, S. A., Amirsadeghi, M., Sukhbaatar, B., Revellame, E. D., Hernandez, R. A., Donaldson, J. R., & French, W. T. (2015) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-014-1564-7 · Cited by 43
Lipolytic and oxidative changes in Iberian dry-cured loin
Muriel, E., Andrés, A. I., Petrón, M. J., et al. (2006) · Meat Science · doi:10.1016/j.meatsci.2006.07.017 · Cited by 43
Evaluation of an Oil-Producing Green Alga Chlorella sp. C2 for Biological DeNO x of Industrial Flue Gases
Zhang, X., Chen, H., Chen, W., Qiao, Y., He, C., & Wang, Q. (2014) · Environmental Science & Technology · doi:10.1021/es5013824 · Cited by 43
Temporal and spatial variations in proximate composition and Condition Index of mussels Mytilus galloprovincialis cultured in suspension in a shellfish farm
Irisarri, J., Fernández-Reiriz, M.-J., & Labarta, U. (2014) · Aquaculture · doi:10.1016/j.aquaculture.2014.09.041 · Cited by 43
Influence of one selectedTisochrysis luteastrain rich in lipids onCrassostrea gigaslarval development and biochemical composition
da Costa, F., Petton, B., Mingant, C., Bougaran, G., Rouxel, C., Quéré, C., Wikfors, G. H., Soudant, P., & Robert, R. (2015) · Aquaculture Nutrition · doi:10.1111/anu.12301 · Cited by 42
Influence of dietary fat on pork eating quality
Alonso, V., Najes, L. M., Provincial, L., et al. (2012) · Meat Science · doi:10.1016/j.meatsci.2012.01.004 · Cited by 43
Effect of dietary starch source on growth performances, digestibility and quality traits of growing pigs
Doti, S., Suárez-Belloch, J., Górriz, M. Á. L., et al. (2014) · Livestock Science · doi:10.1016/j.livsci.2014.03.016 · Cited by 43
Sterculic acid antagonizes 7-ketocholesterol-mediated inflammation and inhibits choroidal neovascularization
Huang, J.-D., Amaral, J., Lee, J. W., Larráyoz, I. M., & Rodríguez, I. R. (2012) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2012.01.013 · Cited by 43
Kinetics of extraction of reducing sugar during blanching of potato slices
Pedreschi, F., Travisany, X., Reyes, C., Troncoso, E., & Pedreschi, R. (2008) · Journal of Food Engineering · doi:10.1016/j.jfoodeng.2008.09.022 · Cited by 43
Proteomic evaluation of myofibrillar carbonylation in chilled fish mince and its inhibition by catechin
Pazos, M., Maestre, R., Gallardo, J. M., et al. (2012) · Food Chemistry · doi:10.1016/j.foodchem.2012.07.109 · Cited by 43
Total and organic mercury, selenium and α‐tocopherol in some deep‐water fish species
Afonso, C. M., Lourenço, H. M., Pereira, C., Martins, M. F., Carvalho, L., Castro, M., & Nunes, M. L. (2008) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.3379 · Cited by 44
The interactive effects of elevated ozone and wheat cultivars on soil microbial community composition and metabolic diversity
Bao, X., Yu, J., Liang, W., Lu, C., Zhu, J., & Li, Q. (2014) · Applied Soil Ecology · doi:10.1016/j.apsoil.2014.11.003 · Cited by 41
Abundance and structural diversity of bacteriohopanepolyols in suspended particulate matter along a river to ocean transect
Sáenz, J., Eglinton, T. I., & Summons, R. E. (2011) · Organic Geochemistry · doi:10.1016/j.orggeochem.2011.05.006 · Cited by 44
Lipidomic profiling of plasma and urine from patients with Gaucher disease during enzyme replacement therapy by nanoflow liquid chromatography-tandem mass spectrometry.
Byeon, S. K., Lee, J.-Y., Lee, J.-S., & Moon, M. (2015) · Journal of Chromatography A · doi:10.1016/j.chroma.2015.01.004 · Cited by 39
Microbial lipid production as biodiesel feedstock from N-acetylglucosamine by oleaginous microorganisms
Zhang, G., French, W. T., Hernandez, R. A., Hall, J., Sparks, D., & Holmes, W. E. (2011) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.2592 · Cited by 43
Membrane Organization and Ionization Behavior of the Minor but Crucial Lipid Ceramide-1-Phosphate
Kooijman, E. E., Sot, J., Montes, L. R., Alonso, A., Gericke, A., KRUIJFF, B. D., Kumar, S., & Goñi, F. M. (2008) · Biophysical Journal · doi:10.1529/biophysj.107.121046 · Cited by 43
Estimation of cis-9, trans-11 conjugated linoleic acid content in UK foods and assessment of dietary intake in a cohort of healthy adults
Mushtaq, S., Mangiapane, E. H., & Hunter, K. A. (2009) · British Journal Of Nutrition · doi:10.1017/s000711450999328x · Cited by 43
Experimental studies and statistical analysis of membrane fouling behavior and performance in microfiltration of microalgae by a gas sparging assisted process
Javadi, N., Ashtiani, F. Z., Fouladitajar, A., & Zenooz, A. M. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2014.03.160 · Cited by 45
Effects of dietary fatty acids on the production and quality of eggs and larvae of Atlantic cod (Gadus morhuaL.)
Røjbek, M., Støttrup, J. G., Jacobsen, C., et al. (2014) · Aquaculture Nutrition · doi:10.1111/anu.12124 · Cited by 43

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks