Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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Microalgae-biorefinery with cascading resource recovery design associated to dairy wastewater treatment
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Dietary cellulose prevents gut inflammation by modulating lipid metabolism and gut microbiota
Kim, Y., Hwang, S. W., Kim, S., Lee, Y., Kim, T., Lee, S.-H., Kim, S. J., Yoo, H. J., Kim, E. N., & Kweon, M. (2020) · Gut Microbes · doi:10.1080/19490976.2020.1730149 · Cited by 197
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Minami, J. K., Morrow, D., Bayley, N. A., Fernandez, E. G., Salinas, J. J., Tse, C., Zhu, H., Su, B., Plawat, R., Jones, A. E., et al. (2023) · Cancer Cell · doi:10.1016/j.ccell.2023.05.001 · Cited by 199
Biochemical changes during sucrose deprivation in higher plant cells.
Journet, E., Bligny, R., & Douce, R. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)35767-8 · Cited by 191
Ceramide-binding and activation defines protein kinase c-Raf as a ceramide-activated protein kinase.
Huwiler, A., Brunner, J., Hummel, R., et al. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.14.6959 · Cited by 191
Effect of Energy and Protein Density of Prepartum Diets on Fat and Protein Metabolism of Dairy Cattle in the Periparturient Period
VandeHaar, M. J., Yousif, G., Sharma, B. K., Herdt, T. H., Emery, R. S., Allen, M. S., & Liesman, J. S. (1999) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(99)75351-8 · Cited by 191
Lipid and fatty acid composition of hyphae and spores of arbuscular mycorrhizal fungi at different growth stages
Olsson, P. A., & Johansen, A. S. (2000) · Mycological Research · doi:10.1017/s0953756299001410 · Cited by 191
Production, degradation, and circulating levels of 1,25-dihydroxyvitamin D in health and in chronic glucocorticoid excess.
Seeman, E., Kumar, R., Hunder, G. G., et al. (1980) · Journal of Clinical Investigation · doi:10.1172/jci109902 · Cited by 191
Association of Platelet-Activating Factor with Primary Acquired Cold Urticaria
Grandel, K. E., Farr, R. S., Wanderer, A. A., et al. (1985) · New England Journal of Medicine · doi:10.1056/nejm198508153130702 · Cited by 191
Platelet activating factor. Stimulation of the lipoxygenase pathway in polymorphonuclear leukocytes by 1-O-alkyl-2-O-acetyl-sn-glycero-3-phosphocholine.
Chilton, F. H., O’Flaherty, J. T., Walsh, C. E., et al. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)83790-0 · Cited by 191
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Robertson, R. C., Guihéneuf, F., Bahar, B., Schmid, M., Stengel, D. B., Fitzgerald, G. F., Ross, R. P., & Stanton, C. (2015) · Marine Drugs · doi:10.3390/md13085402 · Cited by 192
Fenofibrate, a PPARα agonist, has renoprotective effects in mice by enhancing renal lipolysis
Tanaka, Y., Kume, S., Araki, S., Isshiki, K., Chin‒Kanasaki, M., Sakaguchi, M., Sugimoto, T., Koya, D., Haneda, M., Kashiwagi, A., et al. (2011) · Kidney International · doi:10.1038/ki.2010.530 · Cited by 195
Distinguishing human from animal faecal contamination in water: A review
Sinton, L. W., Finlay, R. K., & Hannah, D. J. (1998) · New Zealand Journal of Marine and Freshwater Research · doi:10.1080/00288330.1998.9516828 · Cited by 190
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Lugtenberg, E. J. J., & Peters, R. (1976) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(76)90279-4 · Cited by 189
Long-chain polyunsaturated fatty acid transport across the perfused human placenta.
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Determination of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 in human plasma using high-pressure liquid chromatography
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Boström, K. B., Borén, J., WETTESTEN, M., Sjöberg, A., Bondjers, G., Wiklund, O., Carlsson, P., & Olofsson, S. ‐. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)68945-8 · Cited by 189
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Umhau, J. C., Zhou, W., Carson, R. E., Rapoport, S. I., Polozova, A., DeMar, J. C., Hussein, N., Bhattacharjee, A. K., Ma, K., Esposito, G., et al. (2008) · Journal of Lipid Research · doi:10.1194/jlr.m800530-jlr200 · Cited by 193
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Mahmood, W. M. A. W., Lorwirachsutee, A., Theodoropoulos, C., & González‐Miquel, M. (2019) · ACS Sustainable Chemistry & Engineering · doi:10.1021/acssuschemeng.8b05642 · Cited by 191
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Skorokhod, O. A., Alessio, M., Mordmüller, B. G., Arese, P., & Schwarzer, E. (2004) · The Journal of Immunology · doi:10.4049/jimmunol.173.6.4066 · Cited by 188
Influence of lipid composition of live feed on growth, survival and pigmentation of turbot larvae
Reitan, K. I., Rainuzzo, J. R., & Ôlsen, Y. (1994) · Aquaculture International · doi:10.1007/bf00118531 · Cited by 188
Nisin induces changes in membrane fatty acid composition of Listeria monocytogenes nisin-resistant strains at 10°C and 30°C
Mazzotta, A. S., & Montville, T. J. (1997) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.1997.tb03294.x · Cited by 188
Pseudoalteromonas tunicata sp. nov., a bacterium that produces antifouling agents
Holmström, C., JAMES, S., Neilan, B. A., et al. (1998) · International Journal of Systematic Bacteriology · doi:10.1099/00207713-48-4-1205 · Cited by 188
Lipid Peroxidation and Antioxidant Defense Systems in Rat Liver After Chronic Ethanol Feeding
Kawase, T., Kato, S., & Lieber, C. S. (1989) · Hepatology · doi:10.1002/hep.1840100511 · Cited by 188
PRODUCTION AND CELLULAR LOCALIZATION OF NEUTRAL LONG‐CHAIN LIPIDS IN THE HAPTOPHYTE ALGAE ISOCHRYSIS GALBANA AND EMILIANIA HUXLEYI1
Eltgroth, M. L., Watwood, R. L., & Wolfe, G. V. (2005) · Journal of Phycology · doi:10.1111/j.1529-8817.2005.00128.x · Cited by 183
Solubilisation of poorly water-soluble drugs during in vitro lipolysis of medium- and long-chain triacylglycerols
Christensen, J. Ø., Schultz, K., Møllgaard, B., Kristensen, H. G., & Müllertz, A. (2004) · European Journal of Pharmaceutical Sciences · doi:10.1016/j.ejps.2004.08.003 · Cited by 189
Suicide attempt and n-3 fatty acid levels in red blood cells: A case control study in china
Huan, M., Hamazaki, K., Sun, Y., Itomura, M., Liu, H., Kang, W., Watanabe, S., TERASAWA, K., & Hamazaki, T. (2004) · Biological Psychiatry · doi:10.1016/j.biopsych.2004.06.028 · Cited by 185
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Identification of plasmalogen as the major phospholipid constituent of cardiac sarcoplasmic reticulum
Gross, R. W. (1985) · Biochemistry · doi:10.1021/bi00328a014 · Cited by 187
Alteration of soil microbial communities and water quality in restored wetlands
Bossio, D., Fleck, J. A., Scow, K. M., et al. (2005) · Soil Biology and Biochemistry · doi:10.1016/j.soilbio.2005.09.027 · Cited by 187
Suppression of the Rice Fatty-Acid Desaturase GeneOsSSI2Enhances Resistance to Blast and Leaf Blight Diseases in Rice
Jiang, C., Shimono, M., Maeda, S., et al. (2009) · Molecular Plant-Microbe Interactions · doi:10.1094/mpmi-22-7-0820 · Cited by 187
Wastewater-leachate treatment by microalgae: Biomass, carbohydrate and lipid production
Hernández-García, A., Velasquez‐Orta, S. B., Novelo, E., Yáñez-Noguez, I., Ramírez, I. M., & Ledesma, M. T. O. (2019) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2019.02.052 · Cited by 188
Parathyroid hormone inhibits 25-hydroxyvitamin D3-24-hydroxylase mRNA expression stimulated by 1 alpha,25-dihydroxyvitamin D3 in rat kidney but not in intestine.
Shinki, T., Jin, C. H., Nishimura, A., Nagai, Y., Ohyama, Y., Noshiro, M., Okuda, K., & Suda, T. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)42278-8 · Cited by 186
Phosphatidylserine functions as the major precursor of phosphatidylethanolamine in cultured BHK-21 cells.
Voelker, D. R. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.9.2669 · Cited by 189
Inflammatory Platelet-activating Factor-like Phospholipids in Oxidized Low Density Lipoproteins Are Fragmented Alkyl Phosphatidylcholines
Marathe, G. K., Davies, S. S., Harrison, K. A., et al. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.40.28395 · Cited by 187
Generation of lyso-phospholipids from surfactant in acute lung injury is mediated by type-II phospholipase A2 and inhibited by a direct surfactant protein A-phospholipase A2 protein interaction.
Arbibe, L., Koumanov, K. S., Vial, D., et al. (1998) · Journal of Clinical Investigation · doi:10.1172/jci3236 · Cited by 187
Studies on the mechanism of capacitation II. Evidence for lipid transfer between plasma membrane of rat sperm and serum albumin during capacitation in vitro
Davis, B. K., Byrne, R., & Hungund, B. (1979) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(79)90260-8 · Cited by 187
Lipid-protein interactions in reconstituted membranes containing acetylcholine receptor
Ellena, J. F., Blazing, M. A., & McNamee, M. G. (1983) · Biochemistry · doi:10.1021/bi00293a012 · Cited by 187
Bioethanol from macroalgae: Prospects and challenges
Ramachandra, T. V., & Hebbale, D. (2020) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2019.109479 · Cited by 184
Production of lipids in 10 strains of Chlorella and Parachlorella, and enhanced lipid productivity in Chlorella vulgaris
Přibyl, P., Cepák, V., & Zachleder, V. (2012) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-012-3915-5 · Cited by 185
Targeted alteration of dietary n-3 and n-6 fatty acids for the treatment of chronic headaches: A randomized trial
Ramsden, C. E., Faurot, K. R., Zamora, D., Suchindran, C., MacIntosh, B., Gaylord, S. A., Ringel, A., Hibbeln, J. R., Feldstein, A. E., Mori, T. A., et al. (2013) · Pain · doi:10.1016/j.pain.2013.07.028 · Cited by 187
Seasonal variation in the fatty acid composition of three Mediterranean fish – sardine (Sardina pilchardus), anchovy (Engraulis encrasicholus) and picarel (Spicara smaris)
Zlatanos, S., & Laskaridis, K. (2006) · Food Chemistry · doi:10.1016/j.foodchem.2006.09.013 · Cited by 186
Contributions of altered permeability of intestinal barrier and defecation behavior to toxicity formation from graphene oxide in nematode Caenorhabditis elegans
Wu, Q., Yin, L. H., Li, X., et al. (2013) · Nanoscale · doi:10.1039/c3nr02084c · Cited by 186
Biochemical Properties of Mammalian Neutral Sphingomyelinase2 and Its Role in Sphingolipid Metabolism
Marchesini, N., Luberto, C., & Hannun, Y. A. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m212262200 · Cited by 186
Dietary patterns and blood fatty acid composition in children with attention-deficit hyperactivity disorder in Taiwan
Chen, J., Hsu, S.-F., Hsu, C.-D., Hwang, L.-H., & Yang, S. (2004) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2004.01.008 · Cited by 186
Biosynthesis of hopanoids by sulfate‐reducing bacteria (genus Desulfovibrio )
Blumenberg, M., Krüger, M., Nauhaus, K., Talbot, H. M., Oppermann, B. I., Seifert, R., Pape, T., & Michaelis, W. (2006) · Environmental Microbiology · doi:10.1111/j.1462-2920.2006.01014.x · Cited by 189
Contribution of above- and below-ground plant traits to the structure and function of grassland soil microbial communities
Legay, N., Baxendale, C., Grigulis, K., Krainer, U., Kastl, E.-M., Schloter, M., Bardgett, R. D., Arnoldi, C., Bahn, M., Dumont, M., et al. (2014) · Annals of Botany · doi:10.1093/aob/mcu169 · Cited by 195
Comprehensive Quantitative Analysis of Bioactive Sphingolipids by High-Performance Liquid Chromatography–Tandem Mass Spectrometry
Bielawski, J., Pierce, J. S., Snider, J. M., Rembiesa, B. M., Szulc, Z. M., & Bielawska, A. E. (2009) · Methods in molecular biology · doi:10.1007/978-1-60761-322-0_22 · Cited by 189
Nutrient recovery from industrial wastewater as single cell protein by a co-culture of green microalgae and methanotrophs
Rasouli, Z., Valverde‐Pérez, B., D’Este, M., Francisci, D. D., & Angelidaki, İ. (2018) · Biochemical Engineering Journal · doi:10.1016/j.bej.2018.03.010 · Cited by 189
Lipid signalling drives proteolytic rewiring of mitochondria by YME1L
MacVicar, T., Ohba, Y., Nolte, H., Mayer, F. C., Tatsuta, T., Sprenger, H., Lindner, B., Zhao, Y., Li, J., Bruns, C. J., et al. (2019) · Nature · doi:10.1038/s41586-019-1738-6 · Cited by 187
Localization of 25-hydroxyvitamin D3 1 alpha-hydroxylase and 24-hydroxylase along the rat nephron.
Kawashima, H., Torikai, S., & Kurokawa, K. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.2.1199 · Cited by 186
Stereochemical studies of acyclic isoprenoids—XII. Lipids of methanogenic bacteria and possible contributions to sediments
Risatti, J. B., Rowland, S. J., Yon, D. A., et al. (1984) · Organic Geochemistry · doi:10.1016/0146-6380(84)90030-5 · Cited by 186
Exogenous Anandamide Protects Rat Brain against Acute Neuronal InjuryIn Vivo
van der Stelt, M., Veldhuis, W. B., van Haaften, G. W., Fezza, F., Bisogno, T., Bär, P. R., Veldink, G. A., Vliegenthart, J. F. G., Marzo, V. D., & Nicolay, K. (2001) · Journal of Neuroscience · doi:10.1523/jneurosci.21-22-08765.2001 · Cited by 185

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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