Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 19,601–19,700 of 48,846

Characterization of the metabolites of vitamin D3 in the chick
Haussler, M. R. (1972) · Steroids · doi:10.1016/0039-128x(72)90021-9 · Cited by 32
Reductase, Phosphatase, and Kinase Activities in the Metabolism of Alkyldihydroxyacetone Phosphate and Alkyldihydroxyacetone
Chae, K., Piantadosi, C. A., & Snyder, F. F. C. (1973) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)43412-1 · Cited by 32
Localization of phospholipid biosynthetic enzyme activities in cell-free fractions derived from Rhodopseudomonas sphaeroides.
Cain, B. D., Donohue, T. J., Shepherd, W. D., et al. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)43549-6 · Cited by 32
Incorporation of a naturally occurring fluorescent fatty acid into lipids of cultured mammalian cells.
Rintoul, D. A., & Simoni, R. D. (1977) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)40910-0 · Cited by 32
Disparate Effects of Dietary Fatty Acids on Activity of 5′-Nucleotidase of Rat Liver Plasma Membrane
Zuniga, M. E., Lokesh, B. R., & Kinsella, J. E. (1989) · Journal of Nutrition · doi:10.1093/jn/119.2.152 · Cited by 33
Lipogenesis in liver, lung and adipose tissue of rats fed with oleoylanilide
Casals, C., Garcia-Barreno, P., & Municio, A. M. (1983) · Biochemical Journal · doi:10.1042/bj2120339 · Cited by 32
The effect of dietary lipids on the thermotropic behaviour of rat liver and heart mitochondrial membrane lipids
McMurchie, E. J., Abeywardena, M. Y., Charnock, J. S., & Gibson, R. A. (1983) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(83)90082-2 · Cited by 32
Preparation of erythrocyte ghosts by a glycol-induced osmotic lysis under isoionic conditions
Billah, M. M., FINEAN, J. B., COLEMAN, R., & Michell, R. H. (1976) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(76)90177-2 · Cited by 32
Plasma membranes: Structural analyses of neutral lipids and phospholipids of rat liver
Wood, R. (1970) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(70)90120-7 · Cited by 32
Simultaneous isolation of two platelet membrane fractions: Biochemical, immunological and functional characterization
Enouf, J., Bredoux, R., Boizard, B., et al. (1984) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(84)90378-4 · Cited by 32
Die morphogenese des photosyntheseapparates von Rhodospirillum rubrum II. Die kinetik der thylakoidsynthese nach markieurung der membranen mit [2-14C]azetat
Oelze, J., & Drews, G. (1969) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(69)90009-1 · Cited by 32
Investigation of the Mechanism for Abnormal Renal 25 Hydroxyvitamin D3-1-Hydroxylase Activity in the XLinkedHypMouse*
Tenenhouse, H. S. (1984) · Endocrinology · doi:10.1210/endo-115-2-634 · Cited by 32
Biosynthesis of man-β-G1cNAc-G1cNAc-pyrophosphoryl-polyprenol by a solubilized enzyme from aorta
Heifetz, A. H., & Elbein, A. D. (1977) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(77)91283-9 · Cited by 32
PAF and haematopoiesis: III. Presence and metabolism of platelet-activating factor in human bone marrow
Denizot, Y., Trimoreau, F., Dupuis, F., et al. (1995) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/0167-4889(94)00193-i · Cited by 32
Modification of positional distribution of fatty acids in phosphatidylinositol of rabbit neutrophils stimulated with formylmethionyl-leucyl-phenylalanine
Yano, K., Hattori, H., Imai, A., et al. (1983) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(83)90241-2 · Cited by 32
Phosphatidyl inositol: myo-Inositol exchange enzyme from rat liver: Partial purification and characterization
Takenawa, T., & Egawa, K. (1980) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(80)90467-1 · Cited by 32
Sera containing elevated nonesterified fatty acids from patients with angiographically documented coronary atherosclerosis cause marked lipid accumulation in cultured human arterial smooth muscle-derived cells
Laughton, C. W., Ruddle, D. L., Bedord, C. J., & Alderman, E. L. (1988) · Atherosclerosis · doi:10.1016/0021-9150(88)90174-8 · Cited by 32
Polymorphic behavior of gram-negative bacteria membranes
Borovjagin, V. L., Sabelnikov, A. G., Tarahovsky, Y. S., et al. (1987) · The Journal of Membrane Biology · doi:10.1007/bf02209154 · Cited by 32
Muscarinic, α1 and peptidergic agonists stimulate phosphoinositide hydrolysis and regulate mucin secretion in rat submandibular gland cells
Fleming, N., Bilan, P. T., Sliwinski-Lis, E., et al. (1987) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/bf00583796 · Cited by 32
Influence of membrane lipid fluidity on glucose and uridine facilitated diffusion in human erythrocytes
Read, B. D., & McElhaney, R. N. (1976) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(76)90359-x · Cited by 32
The enzymic hydrolysis and structure of phosphatidyl glycerol
Haverkate, F., Houtsmuller, U. M. T., & Deenen, L. L. M. V. (1962) · Biochimica et Biophysica Acta · doi:10.1016/0006-3002(62)90131-2 · Cited by 32
Components of 25-hydroxyvitamin D in serum of young children in upper midwestern United States
Arnaud, S. S., Matthusen, M., Gilkinson, J., et al. (1977) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/30.7.1082 · Cited by 32
Regulation of phospholipid biosynthesis during cholesterol influx and high density lipoprotein-mediated cholesterol efflux in macrophages
Schmitz, G., Beuck, M., Fischer, H., Nowicka, G., & Robenek, H. (1990) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)42318-1 · Cited by 32
Phorbol ester inhibits arginine vasopressin activation of phospholipase C and promotes contraction of, and prostaglandin production by, cultured mesangial cells
Troyer, D. A., Gonzalez, O. F., Douglas, J. G., et al. (1988) · Biochemical Journal · doi:10.1042/bj2510907 · Cited by 32
Effects of anti-microtubular agents and cycloheximide on the metabolism of chylomicron cholesteryl esters by hepatocyte suspensions
Nilsson, Å. (1977) · Biochemical Journal · doi:10.1042/bj1620367 · Cited by 32
The Comparative Effect of Carrot and Lemon Fiber as a Fat Replacer on Physico-chemical, Textural, and Organoleptic Quality of Low-fat Beef Hamburger
Soncu, E. D., Kolsarici, N., Cicek, N., Ozturk, G. S., Akoglu, I. T., & Arici, Y. K. (2015) · Korean Journal for Food Science of Animal Resources · doi:10.5851/kosfa.2015.35.3.370 · Cited by 31
Effects of nitrogen and phosphorus limitation on lipid accumulation by Chlorella kessleri str. UTEX 263 grown in darkness
Shrestha, N., Dandinpet, K. K., & Schneegurt, M. A. (2020) · Journal of Applied Phycology · doi:10.1007/s10811-020-02144-x · Cited by 31
Strategy study on enhancing lipid productivity of filamentous oleaginous microalgae Tribonema
Hui, W., Wenjun, Z., Wentao, C., Lili, G., & Tianzhong, L. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2016.06.083 · Cited by 31
The presence of lysophosphatidylcholine in chromaffin granules
Arthur, G., & Sheltawy, A. (1980) · Biochemical Journal · doi:10.1042/bj1910523 · Cited by 31
Polar Lipids of Commercial Ulva spp. of Different Origins: Profiling and Relevance for Seaweed Valorization
Moreira, A. S. P., da Costa, E. V., Melo, T., Lopes, D., Pais, A. C. S., Santos, S. A. O., Pitarma, B., Mendes, M., Abreu, H., Collén, P. N., et al. (2021) · Foods · doi:10.3390/foods10050914 · Cited by 36
“samples using the Bligh and Dyer method [41], with slight modifications [29,39].”
Method
Stability of lipids in plasma and serum: Effects of temperature-related storage conditions on the human lipidome
Reis, G. B., Rees, J. C., Ivanova, A., Kuklenyik, Z., Drew, N. M., Pirkle, J. L., & Barr, J. R. (2021) · Journal of Mass Spectrometry and Advances in the Clinical Lab · doi:10.1016/j.jmsacl.2021.10.002 · Cited by 36
Comparative role of microplastics and microalgae as vectors for chlorpyrifos bioacumulation and related physiological and immune effects in mussels
Fernández, B., Campillo, J. A., Chaves-Pozo, E., Bellas, J., León, V. M., & Albentosa, M. (2021) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2021.150983 · Cited by 31
Byproducts of Globe Artichoke and Cauliflower Production as a New Source of Bioactive Compounds in the Green Economy Perspective: An NMR Study
Ingallina, C., Matteo, G. D., Spano, M., Acciaro, E., Campiglia, E., Mannina, L., & Sobolev, A. P. (2023) · Molecules · doi:10.3390/molecules28031363 · Cited by 32
Age-related changes in circadian regulation of the human plasma lipidome
Rahman, S. A., Gathungu, R. M., Marur, V. R., Hilaire, M. A. S., Scheuermaier, K. D., Belenky, M., Struble, J. S., Czeisler, C. A., Lockley, S. W., Klerman, E. B., et al. (2023) · Communications Biology · doi:10.1038/s42003-023-05102-8 · Cited by 31
Inhibiting tau-induced elevated nSMase2 activity and ceramides is therapeutic in an Alzheimer’s disease mouse model
Tallon, C., Bell, B. J., Malvankar, M. M., Deme, P., Nogueras‐Ortiz, C. J., Eren, E., Thomas, A. G., Hollinger, K. R., Pal, A., Mustapić, M., et al. (2023) · Translational Neurodegeneration · doi:10.1186/s40035-023-00383-9 · Cited by 35
The DDHD2-STXBP1 interaction mediates long-term memory via generation of saturated free fatty acids
Akefe, I. O., Saber, S. H., Matthews, B., Venkatesh, B. G., Gormal, R. S., Blackmore, D. G., Alexander, S., Sierecki, E., Gambin, Y., Bertran‐Gonzalez, J., et al. (2024) · The EMBO Journal · doi:10.1038/s44318-024-00030-7 · Cited by 32
“Phospholipid extraction was performed essentially according to the well-established method of Bligh and Dyer (Bligh and Dyer, 1959).”
Tomato seed mucilage-whey protein isolate coating enriched with shallot essential oil: Effect on quality changes of the trout fish fillet during cold storage
Izadi, H., Zandi, M., Rafeiee, G., & Bimakr, M. (2024) · Biocatalysis and Agricultural Biotechnology · doi:10.1016/j.bcab.2024.103149 · Cited by 32
Synergistic effects of oxidative and acid stress on bacterial membranes of Escherichia coli and Staphylococcus simulans
Xie, M., Koch, E. H. W., van Walree, C. A., Sobota, A., Sonnen, A. F. ‐., Killian, J. A., Breukink, E. J., & Lorent, J. H. (2024) · Communications Biology · doi:10.1038/s42003-024-06862-7 · Cited by 37
“Afterward, all samples were incubated with lysostaphin for 1 h at 37 °C and shaken at 220 rpm. Membrane lipids were extracted by the Bligh & Dyer method 80 .”
Membrane structure-responsive lipid scrambling by TMEM63B to control plasma membrane lipid distribution
Miyata, Y., Takahashi, K., Lee, Y., Sultan, C. S., Kuribayashi, R., Takahashi, M., Hata, K., Bamba, T., Izumi, Y., Liu, K., et al. (2024) · Nature Structural & Molecular Biology · doi:10.1038/s41594-024-01411-6 · Cited by 33
“Lipids were extracted according to Bligh and Dyer’s method with some modifications 86 .”
Lipids: Emerging Players of Microglial Biology
Prakash, P., Randolph, C. E., Walker, K. A., & Chopra, G. (2024) · Glia · doi:10.1002/glia.24654 · Cited by 41
Microalgae processing for biodiesel production
Mata, T. M., Martins, A. A., & Caetano, N. S. (2012) · Elsevier eBooks · doi:10.1533/9780857095862.2.204 · Cited by 36
Accumulation of Oxygenated Fatty Acids in Oat Lipids During Storage
Doehlert, D. C., Angelikousis, S., & Vick, B. A. (2010) · Cereal Chemistry · doi:10.1094/cchem-05-10-0074 · Cited by 31
Expression of genes related to quality of Longissimus dorsi muscle meat in Nellore (Bos indicus) and Canchim (5/8 Bos taurus×3/8 Bos indicus) cattle
Giusti, J., Castan, E. P., Dal‐Pai‐Silva, M., et al. (2013) · Meat Science · doi:10.1016/j.meatsci.2013.02.006 · Cited by 31
Application of Response Surface Methodology for the Optimization of Ultrasound-Assisted Extraction of Pomegranate (Punica granatum L.) Seed Oil
Barizão, É. O., Boeing, J. S., Martins, A. C., Visentainer, J. V., & Almeida, V. C. (2015) · Food Analytical Methods · doi:10.1007/s12161-015-0135-5 · Cited by 31
Physiological effects of waterborne lead exposure in spiny dogfish (Squalus acanthias)
Eyckmans, M., Lardon, I., Wood, C. M., et al. (2012) · Aquatic Toxicology · doi:10.1016/j.aquatox.2012.09.004 · Cited by 31
Human baby hair amino acid natural abundance 15N-isotope values are not related to the 15N-isotope values of amino acids in mother’s breast milk protein
Romek, K. M., Julien, M., Frasquet-Darrieux, M., Téa, I., Antheaume, I., Hankard, R., & Robins, R. J. (2013) · Amino Acids · doi:10.1007/s00726-013-1597-7 · Cited by 31
The Conserved Hypothetical Protein Rv0574c Is Required for Cell Wall Integrity, Stress Tolerance, and Virulence of Mycobacterium tuberculosis
Garg, R., Tripathi, D., Kant, S., Chandra, H., Bhatnagar, R., & Banerjee, N. (2014) · Infection and Immunity · doi:10.1128/iai.02274-14 · Cited by 31
Docosahexaenoic Acid Is an Independent Predictor of All-Cause Mortality in Hemodialysis Patients
Hamazaki, K., Terashima, Y., Itomura, M., Sawazaki, S., Inagaki, H., Kuroda, M., Tomita, S., Hirata, H., Inadera, H., & Hamazaki, T. (2010) · American Journal of Nephrology · doi:10.1159/000322952 · Cited by 32
In vitro anti-atherogenic properties of traditional Greek cheese lipid fractions
Tsorotioti, S. E., Nasopoulou, C., Detopoulou, M., Sioriki, E., Demopoulos, C. A., & Zabetakis, I. (2014) · Dairy Science and Technology · doi:10.1007/s13594-014-0161-x · Cited by 34
Burkholderia insulsa sp. nov., a facultatively chemolithotrophic bacterium isolated from an arsenic-rich shallow marine hydrothermal system
Rusch, A., Islam, S., Savalia, P., et al. (2014) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.064477-0 · Cited by 31
Tissue disruption and extraction methods for metabolic profiling of an invertebrate sentinel species
Liebeke, M., & Bundy, J. G. (2011) · Metabolomics · doi:10.1007/s11306-011-0377-1 · Cited by 31
GA application induces alteration in sex ratio and cell death in Jatropha curcas
Makwana, V., Shukla, P., & Robin, P. (2010) · Plant Growth Regulation · doi:10.1007/s10725-010-9457-x · Cited by 31
Growth of fresh-water prawn Macrobrachium tenellum (Smith, 1871) juveniles fed isoproteic diets substituting fish meal by soya bean meal
García‐Ulloa, M., López-Aceves, L. A., Ponce‐Palafox, J. T., et al. (2008) · Brazilian Archives of Biology and Technology · doi:10.1590/s1516-89132008000100008 · Cited by 31
Control of endotoxin release in Escherichia coli fed-batch cultures
Svensson, M., Han, L., Silfversparre, G., et al. (2004) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-004-0377-x · Cited by 31
Development and Characterization of Somatic Hybrids of Ulva reticulata Forsskål (×) Monostroma oxyspermum (Kutz.)Doty
Gupta, V., Kumari, P., & Reddy, C. R. (2015) · Frontiers in Plant Science · doi:10.3389/fpls.2015.00003 · Cited by 29
Characterization of complex lipid mixtures in contaminant exposed JEG-3 cells using liquid chromatography and high-resolution mass spectrometry
Gorrochategui, E., Casas, J., Pérez‐Albaladejo, E., Jáuregui, O., Porte, C., & Lacorte, S. (2014) · Environmental Science and Pollution Research · doi:10.1007/s11356-014-3172-5 · Cited by 31
Linoleic and α-linolenic fatty acid consumption over three generations exert cumulative regulation of hepatic expression of genes related to lipid metabolism
Jacometo, C. B., Schmitt, E., Pfeifer, L. F. M., Schneider, A., Bado, F., da Rosa, F. T., Halfen, S., Pino, F. A. B. D., Loor, J. J., Corrêa, M. N., et al. (2014) · Genes & Nutrition · doi:10.1007/s12263-014-0405-7 · Cited by 31
Discrimination of carbon and nitrogen isotopes from milk to serum and vibrissae in Alaska Steller sea lions (Eumetopias jubatus)
Stegall, V. K., Farley, S. D., Rea, L. D., et al. (2008) · Canadian Journal of Zoology · doi:10.1139/z07-115 · Cited by 31
Expression of common carp growth hormone in the yeast Pichia pastoris and growth stimulation of juvenile tilapia (Oreochromis niloticus)
Li, Y., Bai, J., Jian, Q., Ye, X., Haihua, L., Li, X., Luo, J., & Liang, X. (2002) · Aquaculture · doi:10.1016/s0044-8486(02)00406-4 · Cited by 31
Activation of galactolipid biosynthesis in development of pistils and pollen tubes
Nakamura, Y., Kobayashi, K., & Ohta, H. (2009) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2008.12.018 · Cited by 31
A Comparison of the Lipid-lowering Effects of Four Different n-3 Highly Unsaturated Fatty Acids in HepG2 Cells
Nagao, K., Nakamitsu, K., Ishida, H., Yoshinaga, K., Nagai, T., Mizobe, H., Kojima, K., Yanagita, T., Beppu, F., & Gotoh, N. (2014) · Journal of Oleo Science · doi:10.5650/jos.ess14118 · Cited by 31
Developmental changes in rat surfactant lipidomics in the context of species variability
Bernhard, W., Schmiedl, A., Koster, G., et al. (2007) · Pediatric Pulmonology · doi:10.1002/ppul.20657 · Cited by 31
Through-package fat determination in commercial samples of mayonnaise and salad dressing using time-domain nuclear magnetic resonance spectroscopy and chemometrics
Pereira, F. M. V., Rebellato, A. P., Pallone, J. A. L., & Colnago, L. A. (2014) · Food Control · doi:10.1016/j.foodcont.2014.02.028 · Cited by 31
Texture, color, lipid oxidation and sensory acceptability of gamma-irradiated marinated anchovy fillets
Tomac, A., Cova, M. C., Narvaiz, P., & Yeannes, M. I. (2014) · Radiation Physics and Chemistry · doi:10.1016/j.radphyschem.2014.08.010 · Cited by 31
Adaptation of marine plankton to environmental stress by glycolipid accumulation
Gašparović, B., Godrijan, J., Frka, S., et al. (2013) · Marine Environmental Research · doi:10.1016/j.marenvres.2013.09.009 · Cited by 31
A rapid HPLC post-column reaction analysis for the quantification of ergothioneine in edible mushrooms and in animals fed a diet supplemented with extracts from the processing waste of cultivated mushrooms
Nguyen, T. H., Giri, A., & Ohshima, T. (2012) · Food Chemistry · doi:10.1016/j.foodchem.2012.01.061 · Cited by 31
Antioxidant activity of Hypericum perforatum L. extract in enriched n-3 PUFA pork meat systems during chilled storage
Sánchez-Muniz, F. J., Olivero-David, R., Triki, M., et al. (2012) · Food Research International · doi:10.1016/j.foodres.2012.07.002 · Cited by 31
Rapid Detection and Differentiation of Alicyclobacillus Species in Fruit Juice Using Hydrophobic Grid Membranes and Attenuated Total Reflectance Infrared Microspectroscopy
Grasso, E. M., Yousef, A. E., Castellví, S. D. L., & RODRIGUEZ‐SAONA, L. E. (2009) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf902371j · Cited by 31
An improved RP-HPLC method for simultaneous analyses of squalene and cholesterol especially in aquatic foods
Bavisetty, S. C. B., & Narayan, B. (2015) · Journal of Food Science and Technology · doi:10.1007/s13197-015-1793-4 · Cited by 31
Lung endothelial cell platelet-activating factor production and inflammatory cell adherence are increased in response to cigarette smoke component exposure
Sharma, J. S., Young, D. M., Marentette, J., Rastogi, P., TURK, J. W., & McHowat, J. (2011) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00179.2011 · Cited by 32
Postharvesting population dynamics of the South American sea lion (Otaria byronia) in the southwestern Atlantic
Franco‐Trecu, V., Drago, M., Baladán, C., et al. (2014) · Marine Mammal Science · doi:10.1111/mms.12197 · Cited by 31
Uptake of conjugated linolenic acids and conversion tocis-9,trans-11-ortrans-9,trans-11-conjugated linoleic acids in Caco-2 cells
Schneider, A.-C., Mignolet, É., Schneider, Y., & Larondelle, Y. (2012) · British Journal Of Nutrition · doi:10.1017/s0007114512000608 · Cited by 31
Maribacter spongiicola sp. nov. and Maribacter vaceletii sp. nov., isolated from marine sponges, and emended description of the genus Maribacter
Jackson, S. A., Kennedy, J., Morrissey, J. P., et al. (2015) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.000224 · Cited by 31
Expanding the “toolbox” for studying the biological responses of individual fish to hydropower infrastructure and operating strategies
Hasler, C. T., Pon, L. B., Roscoe, D. W., Mossop, B., Patterson, D. A., Hinch, S. G., & Cooke, S. J. (2009) · Environmental Reviews · doi:10.1139/a09-008 · Cited by 31
Dietary Omega-3 Fatty Acids and Susceptibility to Ventricular Fibrillation
Billman, G. E., Carnes, C. A., Adamson, P. B., Vanoli, E., & Schwartz, P. J. (2012) · Circulation Arrhythmia and Electrophysiology · doi:10.1161/circep.111.966739 · Cited by 32
Multidrug Resistance-Related Protein 1 (MRP1) Function and Localization Depend on Cortical Actin
Hummel, I., Klappe, K., Ercan, C., et al. (2010) · Molecular Pharmacology · doi:10.1124/mol.110.069013 · Cited by 31
Cecembia lonarensis gen. nov., sp. nov., a haloalkalitolerant bacterium of the family Cyclobacteriaceae , isolated from a haloalkaline lake and emended descriptions of the genera Indibacter , Nitritalea and Belliella
Kumar, P. A., Srinivas, T. N. R., Madhu, S., et al. (2011) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.038604-0 · Cited by 31
Different doses of supplemental vitamin D maintain interleukin-5 without altering skeletal muscle strength: a randomized, double-blind, placebo-controlled study in vitamin D sufficient adults
Barker, T., Martins, T. B., Hill, H. R., Kjeldsberg, C. R., Henriksen, V. T., Dixon, B. M., Schneider, E. D., Dern, A., & Weaver, L. K. (2012) · Nutrition & Metabolism · doi:10.1186/1743-7075-9-16 · Cited by 32
Lipidomic profiling of tryptophan hydroxylase 2 knockout mice reveals novel lipid biomarkers associated with serotonin deficiency
Weng, R., Shen, S., Burton, C., Yang, L., Nie, H., Tian, Y., Bai, Y., & Liu, H. (2016) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-015-9256-3 · Cited by 31
Effects of dietary inclusion of benthic matter on feed utilization, digestive and immune enzyme activities of sea cucumber Apostichopus japonicus (Selenka)
Wen, B., Gao, Q.-F., Dong, S., et al. (2016) · Aquaculture · doi:10.1016/j.aquaculture.2016.01.028 · Cited by 31
An analysis of embryo development in palm: interactions between dry matter accumulation and water relations in Pritchardia remota (Arecaceae)
Pérez, H. E., Hill, L. M., & Walters, C. (2012) · Seed Science Research · doi:10.1017/s0960258511000523 · Cited by 31
Evaluation of Different Operational Strategies for Biodiesel Production by Direct Transesterification of Microalgal Biomass
Hidalgo, P., Toro, C. A., Ciudad, G., Schober, S., Mittelbach, M., & Navia, R. (2014) · Energy & Fuels · doi:10.1021/ef500259z · Cited by 31
Mesophilic Acidogenesis of Food Waste-Recycling Wastewater: Effects of Hydraulic Retention Time, pH, and Temperature
Han, G.-S., Shin, S. G., Lee, J., Lee, C., Jo, M.-H., & Hwang, S. (2016) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-016-2147-z · Cited by 30
Elucidation and identification of amino acid containing membrane lipids using liquid chromatography/high‐resolution mass spectrometry
Moore, E. K., Hopmans, E. C., Rijpstra, W. I. C., et al. (2016) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.7503 · Cited by 31
Phospholipid homeostasis maintains cell polarity, development and virulence in metarhizium robertsii
Gao, Q., Lu, Y., Yao, H., et al. (2016) · Environmental Microbiology · doi:10.1111/1462-2920.13408 · Cited by 31
ATG 15 encodes a phospholipase and is transcriptionally regulated by YAP 1 in Saccharomyces cerevisiae
Visvanathan, R., & Rajasekharan, R. (2016) · FEBS Letters · doi:10.1002/1873-3468.12369 · Cited by 31
Stearoyl-CoA desaturase 1 deficiency reduces lipid accumulation in the heart by activating lipolysis independently of peroxisome proliferator-activated receptor α
Bednarski, T., Olichwier, A. J., Opasińska, A., Pyrkowska, A., Gan, A., Ntambi, J. M., & Dobrzyń, P. (2016) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2016.10.005 · Cited by 31
Engineering A shbya gossypii strains for de novo lipid production using industrial by‐products
Lozano‐Martínez, P., Buey, R. M., Ledesma‐Amaro, R., Jiménez, A., & Revuelta, J. L. (2016) · Microbial Biotechnology · doi:10.1111/1751-7915.12487 · Cited by 31
Agro-forestry management of Paulownia plantations and their impact on soil biological quality: The effects of fertilization and irrigation treatments
Moreno, J. L., Bastida, F., Ondoño, S., et al. (2017) · Applied Soil Ecology · doi:10.1016/j.apsoil.2017.05.001 · Cited by 31
Production of low-nitrogen bio-crude oils from microalgae pre-treated with pre-cooled NaOH/urea solution
Hu, Y., Feng, S., Xu, C. C., & Bassi, A. S. (2017) · Fuel · doi:10.1016/j.fuel.2017.06.021 · Cited by 32
Nitrogen enrichment impacts on boreal litter decomposition are driven by changes in soil microbiota rather than litter quality
Maaroufi, N. I., Nordin, A., Palmqvist, K., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-04523-w · Cited by 31
Intrinsic wheat lipid composition effects the interfacial and foaming properties of dough liquor
Salt, L. J., González-Thuillier, I., Chope, G. A., et al. (2017) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2017.08.020 · Cited by 31
Fat, Fatty Acids and Tocopherol Content of Several Walnut Genotypes
Beyhan, Ö., Özcan, A., Ozcan, H., et al. (2017) · Notulae Botanicae Horti Agrobotanici Cluj-Napoca · doi:10.15835/nbha45210932 · Cited by 31
Pseudomonas wadenswilerensis sp. nov. and Pseudomonas reidholzensis sp. nov., two novel species within the Pseudomonas putida group isolated from forest soil
Frasson, D., Opoku, M., Picozzi, T., et al. (2017) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.002035 · Cited by 31
Soil microbial biomass, activity, and community composition as affected by dairy manure slurry applications in grassland production
Shi, Y., Ziadi, N., Hamel, C., et al. (2017) · Applied Soil Ecology · doi:10.1016/j.apsoil.2017.12.022 · Cited by 31
Long-term kinetics of Salmonella Typhimurium ATCC 14028 survival on peanuts and peanut confectionery products
do Nascimento, M. D. S., Carminati, J. A., Morishita, K. N., et al. (2018) · PLoS ONE · doi:10.1371/journal.pone.0192457 · Cited by 31
Fatty acidomics: Evaluation of the effects of thermal treatments on commercial mussels through an extended characterization of their free fatty acids by liquid chromatography – Fourier transform mass spectrometry
Losito, I., Facchini, L., Valentini, A., Cataldi, T. R. I., & Palmisano, F. (2018) · Food Chemistry · doi:10.1016/j.foodchem.2018.02.073 · Cited by 31
Association study between copy number variation and beef fatty acid profile of Nellore cattle
de Lemos, M. V. A., Peripolli, E., Berton, M. P., Feitosa, F. L. B., Olivieri, B. F., Stafuzza, N. B., Tonussi, R. L., Kluska, S., Chiaia, H. L. J., Mueller, L. F., et al. (2018) · Journal of Applied Genetics · doi:10.1007/s13353-018-0436-7 · Cited by 30
Elovl4 and Fa2h expression during rat spermatogenesis: a link to the very-long-chain PUFAs typical of germ cell sphingolipids[S]
Valtierra, F. X. S., Peñalva, D. A., Luquez, J. M., Furland, N. E., Vásquez, C., Reyes, J. G., Aveldaño, M. I., & Oresti, G. M. (2018) · Journal of Lipid Research · doi:10.1194/jlr.m081885 · Cited by 30
Birds, feather-degrading bacteria and preen glands: the antimicrobial activity of preen gland secretions from turkeys (Meleagris gallopavo) is amplified by keratinase
Braun, M. S., Sporer, F., Zimmermann, S., et al. (2018) · FEMS Microbiology Ecology · doi:10.1093/femsec/fiy117 · Cited by 31

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks