Effects of nitrogen and phosphorus limitation on lipid accumulation by Chlorella kessleri str. UTEX 263 grown in darkness
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Growing algae in darkness for biodiesel production eliminates the challenges of evaporation and light penetration reported for open ponds and the costs and fouling that plague photobioreactors. The current study demonstrated that Chlorella kessleri str. UTEX 263 could grow heterotrophically in the dark on pure sugars or lignocellulosic hydrolysates of plant biomass. Hydrolysates of a prairie grass native to Kansas, Big bluestem (Andropogon gerardii), supported the growth of C. kessleri in the dark. Nitrogen limitation stimulated the accumulation of biodiesel lipids by 10-fold in heterotrophic cultures grown on pure sugars or Big bluestem hydrolysate. Limiting P in the growth medium also was shown to increase cellular lipid accumulation in C. kessleri. Iron limitation was not sufficient to increase cellular lipid content. Crude biomass extracts may have levels of N that can't be easily removed, which are high enough to relieve N limitations in growth media. This initial study suggests that P might be more easily removed from biomass extracts than N for increasing cellular lipid production by nutrient limitation and further that native prairie grasses are potentially suitable as sources of lignocellulosic sugars.
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Phosphorus deficiency can increase the accumulation of lipids and fatty acids, and the changes of fatty acid composition are similar to that under nitrogen deficiency.
“It has been found that phosphorus deficiency can increase the accumulation of lipids and FAs [113, 119, 124], and the changes of FAs composition were similar to that under nitrogen deficiency.”
From Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities (2024) · cites this paper as [119]
The Results section, “Lipid content under nutrient limitation” (page unavailable), reports increased cellular lipid content under phosphorus limitation. But the paper’s fatty-acid profiles compare biomass carbon sources, not phosphorus and nitrogen limitation; our copy does not support the claimed similarity in fatty-acid composition changes. (LLM, conf=0.88)
Experimental — accuracy not yet measuredThe growth of microalgae cells under nitrogen deficiency is inhibited usually, manifested as a decrease in chlorophyll content and yellowing cultures.
“However, the growth of microalgae cells under nitrogen deficiency is inhibited usually, which is manifested as a decrease in chlorophyll content and yellowing cultures [119].”
From Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities (2024) · cites this paper as [119]
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Eldiehy, K. S. H., Bardhan, P., Borah, D., Rather, M. A., Chutia, H., Raj, G. V. S. B., Mandal, M., & Deka, D. (2022) · Journal of Applied Phycology · doi:10.1007/s10811-022-02836-6 · Cited by 3 · see citing works
Merino, F., Espinoza, S. M., Garay, J. C., Vigo, L. C., Huamancondor-Paz, Y. P., Choque-Quispe, Y., Buleje-Campos, D., Choque-Quispe, D., Rodriguez-Cardenas, L., Rojas, G. B. S., et al. (2024) · Scientific Reports · doi:10.1038/s41598-024-52919-2 · Cited by 3 · see citing works
Algal Oil as a Low‐Cost Feedstock for Biodiesel Production
Chhandama, M. V. L., Satyan, K. B., & Rokhum, S. L. (2022) · Wiley eBooks · doi:10.1002/9781119771364.ch4
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Microalgal Technology Challenges
Zaharieva, M. M., Scheifelle, F. B., Kroumov, A. D., & Najdenski, H. M. (2026) · Sustainable materials and technology · doi:10.1007/978-981-95-2597-3_4
On the Use of Chlorella Kessleri Microalgae to Achieve Sustainable Development Goals
Velmozhina, K., Shinkevich, P., Politaeva, N., & Чусов, А. Н. (2026) · Springer proceedings in earth and environmental sciences · doi:10.1007/978-3-031-99762-4_7
Zhang, J., Lu, S., Gao, Y., Jiang, Y., Zhang, H., & Perré, P. (2026) · Environmental Technology & Innovation · doi:10.1016/j.eti.2026.104851
Microalgae for bioregenerative life support in space
Revellame, E. D., Gatdula, K. M., Aguda, R. M., Fortela, D. L. B., Holmes, W. E., Hernandez, R. A., & Zappi, M. E. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-34082-6.00044-1
- Authors
- Shrestha, N., Dandinpet, K. K., & Schneegurt, M. A.
- Affiliations
- Wichita State University (US)
- Corresponding author
- Schneegurt, Mark A.
- ORCID
- Schneegurt, Mark A.
- Venue
- Journal of Applied Phycology
- Published
- 2020-06-08
- Volume
- 32
- Issue
- 5
- Pages
- 2795-2805
- ISSN
- 0921-8971 (ISSN-L)
- 1573-5176
- PMC
- PMC7879712
- OpenAlex
- W3035085431
- Cited by
- 31
- References
- 83
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 5 · 2023: 6 · 2022: 8 · 2021: 4
- FWCI
- 0.89
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Applied Phycology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Holmes Museum Of Anthropology (Wichita State University) (submittedVersion)
- Funders
- Directorate for Biological Sciences (EPS-0903806)
- National Institute of General Medical Sciences (P20 GM103418)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Keywords
- Chlorella kessleri, lipid accumulation, nitrogen limitation, phosphorus limitation, heterotrophic growth, dark cultivation, biodiesel, lignocellulosic hydrolysates, Andropogon gerardii, prairie grasses, iron limitation, lignocellulosic sugars
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