Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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Effect of partial replacement of meat by carrot on physicochemical properties and fatty acid profile of fresh turkey sausages: a chemometric approach
Rocchetti, G., Pateiro, M., Campagnol, P. C. B., et al. (2020) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.10560 · Cited by 21
High-fat-diet induced obesity increases the proportion of linoleic acyl residues in dam serum and milk and in suckling neonate circulation
Suárez-Trujillo, A., Huff, K., Ferreira, C. R., et al. (2020) · Biology of Reproduction · doi:10.1093/biolre/ioaa103 · Cited by 21
“Plates were read with a multimode microplate reader (Spark 10M, TECAN, Männedorf, Switzerland) at 540 nm. Serum and milk lipid extraction for lipidomics The Bligh & Dyer [12] method was used to extract lipids from serum and milk samples.”
Method
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“Fatty acid analysis of ghee and ghrita formulations were done by modified Bligh and Dyer method [29,30].”
Method
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“Total lipids (TL)* were extracted and measured [22], and the TL extracts used both to measure enzymatic lipid hydrolysis in the form of free fatty acids (FFA) [23] with modifications [24] and phospholipid (PL) content [25].”
Method
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Olea‐Ozuna, R. J., Poggio, S., EdBergström, Quiroz‐Rocha, E., García‐Soriano, D. A., Sahonero‐Canavesi, D. X., Padilla‐Gómez, J., Martínez‐Aguilar, L., López-Lara, I. M., THOMAS‐OATES, J. E., et al. (2020) · Environmental Microbiology · doi:10.1111/1462-2920.15280 · Cited by 22
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Influence of light quality on Chlorella growth, photosynthetic pigments and high-valued products accumulation in coastal saline-alkali leachate
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Bhattacharjya, R., Singh, P. K., Mishra, B., Saxena, A., & Tiwari, A. (2021) · Aquaculture Research · doi:10.1111/are.15143 · Cited by 20
Effects of short feed restriction cycles in Piaractus brachypomus juveniles
Fávero, G. C., dos Santos, F. A. C., Júlio, G. S. D. C., et al. (2021) · Aquaculture · doi:10.1016/j.aquaculture.2021.736465 · Cited by 21
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Antioxidants enhance lipid productivity in Heveochlorella sp. Yu
Cui, N., Xiao, J., Feng, Y., Zhao, Y., Yu, X., Xu, J., Li, T., & Zhao, P. (2021) · Algal Research · doi:10.1016/j.algal.2021.102235 · Cited by 21

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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