Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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“Lipid metabolites were extracted from ~50 mg of frozen liver tissue using a modified Bligh and Dyer method [43].”
Phylogenetic identification and assessment of the nutritional value of different diets for a copepod species isolated from Eastern Harbor coastal region
El‐Khodary, G. M., Mona, M. M., El‐Sayed, H. S., et al. (2020) · The Egyptian Journal of Aquatic Research · doi:10.1016/j.ejar.2020.03.003 · Cited by 18
Functional Characterization of Lysophosphatidylcholine: Acyl-CoA Acyltransferase Genes From Sunflower (Helianthus annuus L.)
Mapelli-Brahm, A., Sánchez, R., Pan, X., et al. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.00403 · Cited by 18
“The total lipids were extracted (Bligh and Dyer, 1959), evaporated under nitrogen, and dissolved in 1 ml chloroform.”
Method
Effects of acidified pig slurry application on soil chemical and microbiological properties under field trial conditions
Edesi, L., Talve, T., Akk, E., et al. (2020) · Soil and Tillage Research · doi:10.1016/j.still.2020.104650 · Cited by 18
Evaluation of Chlorella sorokiniana cultivated in outdoor photobioreactors for biodiesel production
Menegazzo, M. L., Nascimento, V. M., Hestekin, C. N., Hestekin, J., & Fonseca, G. G. (2020) · Biofuels · doi:10.1080/17597269.2020.1763094 · Cited by 17
“The lipid content of this biomass produced at least 3.65 ± 0.12 g of lipids, which corresponds to 20.08 ± 1.53% lipids using the conventional method of Bligh & Dyer [47] and the protein content was 50.44 ± 1.11%.”
MethodInfluential
Kinetics of landfill leachate remediation and microalgae metabolism as well as energy potential evaluation
Chang, H., Zou, Y., Hu, R., Zhong, N., Zhao, S., Zheng, Y., Qin, Y., & Feng, C. (2020) · Journal of Cleaner Production · doi:10.1016/j.jclepro.2020.122413 · Cited by 19
Editing of phosphatidic acid and phosphatidylethanolamine by acyl-CoA: lysophospholipid acyltransferases in developing Camelina sativa seeds
Klińska, S., Jasieniecka‐Gazarkiewicz, K., Demski, K., et al. (2020) · Planta · doi:10.1007/s00425-020-03408-z · Cited by 18
Lipid profile of Trichinella papuae muscle-stage larvae
Mangmee, S., Adisakwattana, P., Tipthara, P., Simanon, N., Sonthayanon, P., & Reamtong, O. (2020) · Scientific Reports · doi:10.1038/s41598-020-67297-8 · Cited by 19
High Light Induced Alka(e)ne Biodegradation for Lipid and Redox Homeostasis in Cyanobacteria
Qiao, Y., Wang, W., & Lü, X. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.01659 · Cited by 18
“The culture was extracted for 1 h at room temperature with 4 mL of chloroform:methanol (1:1, v/v) (Bligh and Dyer, 1959).”
Method
Phospholipidomics of peripheral blood mononuclear cells (PBMCs): the tricky case of children with autism spectrum disorder (ASD) and their healthy siblings
Ventura, G., Calvano, C. D., Porcelli, V., Palmieri, L., Giacomo, A. D., Yun Xu, A., Goodacre, R., Palmisano, F., & Cataldi, T. R. I. (2020) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-020-02817-z · Cited by 18
“Lipids were extracted from PBMC following the Bligh and Dyer protocol [27].”
Method
Phospholipid Arachidonic Acid Remodeling During Phagocytosis in Mouse Peritoneal Macrophages
Gil‐de‐Gómez, L., Monge, P., Rodríguez, J. P., Astudillo, A. M., Balboa, M. A., & Balsinde, J. (2020) · Biomedicines · doi:10.3390/biomedicines8080274 · Cited by 20
“Total lipids from approximately 107 cells were extracted according to Bligh and Dyer [38].”
Method
Relationship of morphometrics, total carotenoids, and total lipids with activity and sexual and spatial features in Euphausia superba
Lorda, J. F., & Ceccaldi, H. J. (2020) · Scientific Reports · doi:10.1038/s41598-020-69780-8 · Cited by 18
“Only two Male II individuals were found in transect 62°S + 63°S; however, the number of individuals for transect 62°S + 63°S was smaller (49) than for transect 64°S (70).”
Background
Intrahepatic Expression of Fatty Acid Translocase CD36 Is Increased in Obstructive Sleep Apnea
Rey, E., Pozo-Maroto, E. D., Marañón, P., Beeler, B., García‐García, Y., Landete, P., Isaza, S. C., Farré, R., García‐Monzón, C., Almendros, I., et al. (2020) · Frontiers in Medicine · doi:10.3389/fmed.2020.00450 · Cited by 18
“Quantitative Analysis of Hepatic Triglycerides Triglycerides (TGs) were extracted as described previously (25).”
Method
Characterization of a high lipid-producing thermotolerant marine photosynthetic pico alga from genus Picochlorum (Trebouxiophyceae)
Mucko, M., Padisák, J., Udovič, M. G., Pálmai, T., Novak, T., Medić, N., Gašparović, B., Štefanić, P. P., Orlić, S., & Ljubešić, Z. (2020) · European Journal of Phycology · doi:10.1080/09670262.2020.1757763 · Cited by 18
Sensitivity of Atlantic salmon to the pesticide pirimiphos-methyl, present in plant-based feeds
Berntssen, M. H. G., Rosenlund, G., Garlito, B., Amlund, H., Sissener, N. H., Bernhard, A., & Sanden, M. (2020) · Aquaculture · doi:10.1016/j.aquaculture.2020.735825 · Cited by 18
Polarized human cholangiocytes release distinct populations of apical and basolateral small extracellular vesicles
Davies, B. A., Morton, L. A., Jefferson, J. R., Rozeveld, C. N., Doskey, L. C., LaRusso, N. F., & Katzmann, D. J. (2020) · Molecular Biology of the Cell · doi:10.1091/mbc.e19-03-0133 · Cited by 17
“Total lipids were extracted from exosome preps according to the Bligh and Dyer method (Bligh and Dyer, 1959) scaled to accommodate an aqueous volume of 40 µl using 5 × 50 mm disposable culture tubes sealed with Teflon tape.”
Microwave-assisted pretreatment of harmful algal blooms for microbial oil-centered biorefinery approach
Kumar, V., Arora, N., Pandey, S., et al. (2020) · Biomass Conversion and Biorefinery · doi:10.1007/s13399-020-00941-5 · Cited by 18
“The intracellular lipids were extracted by the modified Bligh and Dyer method from the resultant microalga and yeast biomas cultivated in both the hydrolysates [21, 23].”
Method
Systemic deficiency of mouse arachidonate 15‐lipoxygenase induces defective erythropoiesis and transgenic expression of the human enzyme rescues this phenotype
Rademacher, M., Kühn, H., & Borchert, A. (2020) · The FASEB Journal · doi:10.1096/fj.202000408rr · Cited by 17
Maximization of microbial oil containing polyunsaturated fatty acid production by Umbelopsis (Mortierella) isabellina
Somacal, S., Pinto, V. S., Vendruscolo, R. G., Somacal, S., Wagner, R. C., Ballus, C. A., Kuhn, R. C., Mazutti, M. A., & de Menezes, C. R. (2020) · Biocatalysis and Agricultural Biotechnology · doi:10.1016/j.bcab.2020.101831 · Cited by 18
Comprehensive lipidomic profiling in serum and multiple tissues from a mouse model of diabetes
Chen, Z., Liang, Q., Wu, Y., Gao, Z., Kobayashi, S., Patel, J., Li, C., Cai, F., Zhang, Y., Liang, C., et al. (2020) · Metabolomics · doi:10.1007/s11306-020-01732-9 · Cited by 19
Harnessing pongamia shell hydrolysate for triacylglycerol agglomeration by novel oleaginous yeast Rhodotorula pacifica INDKK
Kumar, K. K., Deeba, F., Sauraj, Negi, Y. S., & Gaur, N. A. (2020) · Biotechnology for Biofuels · doi:10.1186/s13068-020-01814-9 · Cited by 18
Food waste hydrolysate as fermentation medium: Comparison of pre-treatment methods
Chua, G. K., Tan, F. H. Y., Chew, F. N., et al. (2020) · Materials Today Proceedings · doi:10.1016/j.matpr.2020.10.399 · Cited by 18
Stimulation of Lipid Extraction Efficiency from Sewage Sludge for Biodiesel Production through Hydrothermal Pretreatment
Lee, J., Choi, O. K., Oh, D., Lee, K., Park, K. Y., & Kim, D. (2020) · Energies · doi:10.3390/en13236392 · Cited by 19
“The triglycerides (TG) and phospholipid fatty acids (PFLAs), which are the major lipid components of the sludge, are able to be converted to biodiesel fatty acid methyl esters (FAMEs) via transesterification [23].”
BackgroundMethod
Elovl2 Is Required for Robust Visual Function in Zebrafish
Dasyani, M., Gao, F., Xu, Q., Fossan, D. V., Zhang, E., Pinto, A. F. M., Saghatelian, A., Skowronska‐Krawczyk, D., & Chao, D. L. (2020) · Cells · doi:10.3390/cells9122583 · Cited by 18
Hypolipidemic and Hepatoprotective Effects of Polysaccharides Extracted from Liriope spicata Var. Prolifera in C57BL/6J Mice with High‐Fat Diet‐Induced Hyperlipidemia
Liu, Y., Xiang, Z.-N., Chen, C., Wan, L.-S., & Chen, J. (2020) · Evidence-based Complementary and Alternative Medicine · doi:10.1155/2020/8013189 · Cited by 20
Interactions between soil microbial communities and agronomic behavior in a mandarin crop subjected to water deficit and irrigated with reclaimed water
Abadía, J., Bastida, F., Romero‐Trigueros, C., Bayona, J. M., Vera, A., García, C. P., Alarcón, J. J., & Nicolás, E. (2021) · Agricultural Water Management · doi:10.1016/j.agwat.2021.106749 · Cited by 17
A Validated Method for Quantification of Fatty Acids Incorporated in Human Plasma Phospholipids by Gas Chromatography–Triple Quadrupole Mass Spectrometry
Schött, H.-F., Konings, M. C. J. M., Schrauwen‐Hinderling, V. B., Mensink, R. P., & Plat, J. (2021) · ACS Omega · doi:10.1021/acsomega.0c03874 · Cited by 18
GC-MS/MS analysis of metabolites derived from a single human blastocyst
Inoue, N., Nishida, Y., Harada, E., et al. (2021) · Metabolomics · doi:10.1007/s11306-021-01770-x · Cited by 18
“Frozen embryo medium samples were thawed, phosphatebuffered saline was added to each 50 μl culture sample to obtain a total volume of 200 μl, and lipids were extracted using the method reported by Bligh and Dyer (1959).”
Method

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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