Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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“The samples were dried with a current of nitrogen and re-suspended in 200 μl chloroform and stored at − 80 C (Bligh and Dyer 1959; Kates et al., 1986).”
Method
Particulate organic matter release below melting sea ice (Terra Nova Bay, Ross Sea, Antarctica): Possible relationships with zooplankton
Cau, A., Ennas, C., Moccia, D., Mangoni, O., Bolinesi, F., Saggiomo, M., Granata, A., Guglielmo, L., Swadling, K. M., & Pusceddu, A. (2021) · Journal of Marine Systems · doi:10.1016/j.jmarsys.2021.103510 · Cited by 16
Effects of protein source and lipid supplementation on conservation and feed value of total mixed ration silages for finishing beef cattle
Lazzari, G., Poppi, A. C. D. O., Machado, J., Bueno, A. V. I., Gomes, A. L. M., Jobim, C. C., & Daniel, J. L. P. (2021) · Journal of Animal Science · doi:10.1093/jas/skab032 · Cited by 18
Deadwood Reduces the Variation in Soil Microbial Communities Caused by Experimental Forest Gaps
Perreault, L., Forrester, J. A., Mladenoff, D. J., et al. (2021) · Ecosystems · doi:10.1007/s10021-021-00624-w · Cited by 16
Evaluation of muscle lipid extraction and non‐lethal fin tissue use for carbon, nitrogen, and sulfur isotope analyses in adult salmonids
Larocque, S. M., Fisk, A. T., & Johnson, T. B. (2021) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.9093 · Cited by 16
Bioaccumulation of phenolic endocrine disruptors in the clam Rangia cuneata: Storage in shells and influence of size and sex
Graca, B., Rychter, A., Staniszewska, M., Smolarz, K., Sokołowski, A., & Bodziach, K. (2021) · Environmental Research · doi:10.1016/j.envres.2021.111181 · Cited by 16
Modulation of fatty acid profiles by global and local ocean change drivers in the ragworm Hediste diversicolor: implications for aquaculture production
Fernandes, J. F., Ricardo, F., Jerónimo, D., et al. (2021) · Aquaculture · doi:10.1016/j.aquaculture.2021.736871 · Cited by 16
Characterization of key enzymes involved in triacylglycerol biosynthesis in mycobacteria
Asis, A. C., Savoretti, F., Cabruja, M., Gramajo, H. C., & Gago, G. (2021) · Scientific Reports · doi:10.1038/s41598-021-92721-y · Cited by 16
Improvement of water quality, survivality, growth performance, and proximate composition of Penaeus monodon postlarvae through immobilizing Tetraselmis chuii
Khatoon, H., Kwan, P. P., Banerjee, S., Mahmud, A. I., Rahman, M. R., Mian, S., Minhaz, T. M., & Hossain, S. (2021) · Bioresource Technology Reports · doi:10.1016/j.biteb.2021.100755 · Cited by 16
Stratum Corneum Function: A Structural Study with Dynamic Synchrotron X-ray Diffraction Experiments
Hatta, I., Nakazawa, H., Ohta, N., Uchino, T., & Yanase, K. (2021) · Journal of Oleo Science · doi:10.5650/jos.ess21159 · Cited by 14
Lipidomics in Biomarker Research
Hornemann, T. (2021) · Handbook of experimental pharmacology · doi:10.1007/164_2021_517 · Cited by 16
Exposure to cigarette smoke precipitates simple hepatosteatosis to NASH in high-fat diet fed mice by inducing oxidative stress
Fouda, S., Khan, A. K., Chan, S. M. H., Mahzari, A., Zhou, X., Qin, C. X., Vlahos, R., & Ye, J. (2021) · Clinical Science · doi:10.1042/cs20210628 · Cited by 18
Dairy Processing Affects the Gut Digestion and Microecology by Changing the Structure and Composition of Milk Fat Globules
Zhou, X., Hadiatullah, H., Guo, T., Yao, Y., Li, C., & Wang, X. (2021) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.1c04482 · Cited by 16
Changes in the microbiological, physicochemical properties of Chinese traditional fermented Suan rou at ripening fermentation
Zeng, X., Meng, J., Zhang, W., He, L., Deng, L., & Ye, C. (2021) · Food Science & Nutrition · doi:10.1002/fsn3.2095 · Cited by 16
“Determination of FFA was performed according to the methods of (Bligh & Dyer, 1959).”
Method
Effects of dietary linolenic acid to linoleic acid ratio on growth performance, proximate composition and fatty acid contents of pacu ( Piaractus mesopotamicus )
Gonçalves, L. U., Cortegano, C. A. A., Barone, R. S. C., Lorenz, E. K., & Cyrino, J. E. P. (2021) · Aquaculture Research · doi:10.1111/are.15536 · Cited by 17
Dietary protein level influenced reproductive development of hoven’s carp Leptobarbus hoevenii female broodstock
Iqbal, M. F., Liew, H. J., & Rahmah, S. (2021) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2021.115112 · Cited by 16
Nutritional Value of Sea Urchin Roe (Strongylocentrotidae)—Study of Composition and Storage Conditions
Matveeva, V. A., Shulgina, L. V., Prikhodko, Y., Шульгин, Ю. П., Madej, K., & Piekoszewski, W. (2021) · Separations · doi:10.3390/separations8100174 · Cited by 18
“Sea urchin roe bio testing using Tetrahymena pyriformis infusoria [20] as an indicator showed that the specific biological value was 102.”
Method

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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