Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 3,001–3,100 of 48,847

Asymmetric distribution of arachidonic acid in the plasma membrane of human platelets A determination using purified phospholipases and a rapid method for membrane isolation
Perret, B., Chap, H., & Douste‐Blazy, L. (1979) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(79)90131-7 · Cited by 144
Effect of the Type of Bean, Processing, and Geographical Location on the Biodiesel Produced from Waste Coffee Grounds
Jenkins, R. W., Stageman, N. E., Fortune, C. M., & Chuck, C. J. (2014) · Energy & Fuels · doi:10.1021/ef4022976 · Cited by 142
The characteristics of TAG and EPA accumulation in Nannochloropsis oceanica IMET1 under different nitrogen supply regimes
Meng, Y., Jiang, J., Wang, H., Cao, X., Xue, S., Yang, Q. K., & Wang, W. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2014.12.012 · Cited by 143
Feeding Iberian pigs with acorns and grass in either free-range or confinement affects the carcass characteristics and fatty acids and tocopherols accumulation in Longissimus dorsi muscle and backfat
Rey, A. I., Daza, A., Lopez-Carrasco, C., et al. (2005) · Meat Science · doi:10.1016/j.meatsci.2005.10.018 · Cited by 143
Bulk RNA degradation by nitrogen starvation‐induced autophagy in yeast
Huang, H., Kawamata, T., Horie, T., Tsugawa, H., Nakayama, Y., Ohsumi, Y., & Fukusaki, E. (2014) · The EMBO Journal · doi:10.15252/embj.201489083 · Cited by 151
Membrane-Mediated Induction and Sorting of K-Ras Microdomain Signaling Platforms
Weise, K., Kapoor, S., Denter, C., Nikolaus, J., Opitz, N., Koch, S., Triola, G., Herrmann, A., Waldmann, H., & Winter, R. (2010) · Journal of the American Chemical Society · doi:10.1021/ja107532q · Cited by 143
Inflammation-associated lysophospholipids as ligands for CD1d-restricted T cells in human cancer
Chang, D., Deng, H., Matthews, P., Krasovsky, J., Ragupathi, G., Špíšek, R., Mazumder, A., Vesole, D. H., Jagannath, S., & Dhodapkar, M. V. (2008) · Blood · doi:10.1182/blood-2008-04-149831 · Cited by 143
Distinct lipid compositions of two types of human prostasomes
Brouwers, J. F. H. M., Aalberts, M., Jansen, J. W. A., van Niel, G., Wauben, M. H. M., Stout, T. A. E., Helms, J. B., & Stoorvogel, W. (2013) · PROTEOMICS · doi:10.1002/pmic.201200348 · Cited by 151
A mitochondrial phosphatase required for cardiolipin biosynthesis: the PGP phosphatase Gep4
Osman, C., Haag, M., Wieland, F., Brügger, B., & Langer, T. (2010) · The EMBO Journal · doi:10.1038/emboj.2010.98 · Cited by 142
New sample preparation approach for mass spectrometry-based profiling of plasma results in improved coverage of metabolome
Yang, Y., Cruickshank-Quinn, C. I., Armstrong, M., et al. (2013) · Journal of Chromatography A · doi:10.1016/j.chroma.2013.04.030 · Cited by 143
Intestinal Farnesoid X Receptor Controls Transintestinal Cholesterol Excretion in Mice
de Boer, J. F., Schonewille, M., Boesjes, M., Wolters, H., Bloks, V. W., Bos, T., van Dijk, T. H., Jurdzinski, A., Boverhof, R., Wolters, J. C., et al. (2017) · Gastroenterology · doi:10.1053/j.gastro.2016.12.037 · Cited by 142
Modeling Exposure in the Tox21 in Vitro Bioassays
Fischer, F. C., Henneberger, L., König, M., Bittermann, K., Linden, L., Goss, K., & Escher, B. I. (2017) · Chemical Research in Toxicology · doi:10.1021/acs.chemrestox.7b00023 · Cited by 152
Assessment of the stability of sheep sausages with the addition of different concentrations of Origanum vulgare extract during storage
Fernandes, R. P., Trindade, M. A., Lorenzo, J. M., et al. (2017) · Meat Science · doi:10.1016/j.meatsci.2017.11.018 · Cited by 143
Sirtuin 5 Regulates Proximal Tubule Fatty Acid Oxidation to Protect against AKI
Chiba, T., Peasley, K., Cargill, K. R., Maringer, K. V., Bharathi, S. S., Mukherjee, E., Zhang, Y., Holtz, A. N., Basisty, N., Yagobian, S. D., et al. (2019) · Journal of the American Society of Nephrology · doi:10.1681/asn.2019020163 · Cited by 143
Free, and Not Total, 1,25-Dihydroxyvitamin D Regulates 25-Hydroxyvitamin D Metabolism by Keratinocytes
Bikle, D. D., & GEE, E. (1989) · Endocrinology · doi:10.1210/endo-124-2-649 · Cited by 143
Phosphatidate Phosphohydrolase Catalyzes the Hydrolysis of Ceramide 1-Phosphate, Lysophosphatidate, and Sphingosine 1-Phosphate
Waggoner, D. W., Gómez‐Muñoz, A., Dewald, J. W., et al. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.28.16506 · Cited by 143
Membrane-bound nitrite oxidoreductase of Nitrobacter: evidence for a nitrate reductase system
Sundermeyer, H., Meyer, W., Warninghoff, B., & Bock, E. (1984) · Archives of Microbiology · doi:10.1007/bf00454918 · Cited by 147
Fatty acid Content in Whole-cell Hydrolysates and Phospholipid and Phospholipid Fractions of Pseudomonads: a Taxonomic Evaluation
Vancanneyti, M., Witt, S., Abraham, W., Kersters, K., & Fredrickson, H. L. (1996) · Systematic and Applied Microbiology · doi:10.1016/s0723-2020(96)80025-7 · Cited by 142
A Mammalian Homolog of the Yeast LCB1 Encodes a Component of Serine Palmitoyltransferase, the Enzyme Catalyzing the First Step in Sphingolipid Synthesis
Hanada, K., Hara, T., Nishijima, M., et al. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.51.32108 · Cited by 143
Effect of lipid oxidation and frozen storage on muscle proteins of Atlantic mackerel (Scomber scombrus)
Saeed, S., & Howell, N. K. (2002) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.1080 · Cited by 143
Oxylipin profiling in pathogen-infected potato leaves
Göbel, C., Feußner, I., Hámberg, M., et al. (2002) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/s1388-1981(02)00268-8 · Cited by 143
Analysis of Volatiles in Meat from Iberian Pigs and Lean Pigs after Refrigeration and Cooking by Using SPME-GC-MS
Estévez, M., Morcuende, D., Ventanas, S., & Cava, R. (2003) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf026218h · Cited by 144
Fatty liver in familial hypobetalipoproteinemia: triglyceride assembly into VLDL particles is affected by the extent of hepatic steatosis
Schonfeld, G., Patterson, B. W., Yablonskiy, D. A., Tanoli, T. S. K., Averna, M. R., Elias, N., Yue, P., & Ackerman, J. J. H. (2003) · Journal of Lipid Research · doi:10.1194/jlr.m200342-jlr200 · Cited by 143
A mitochondrial pool of sphingomyelin is involved in TNFα-induced Bax translocation to mitochondria
Birbes, H., Luberto, C., Hsu, Y., et al. (2005) · Biochemical Journal · doi:10.1042/bj20041627 · Cited by 143
Ultrastructure and Lipid Alterations Induced by Cadmium in Tomato (Lycopersicon esculentum) Chloroplast Membranes
Djebali, W., Zarrouk, M., Brouquisse, R., et al. (2005) · Plant Biology · doi:10.1055/s-2005-837696 · Cited by 143
Changes in cellular energy budget as a measure of whole effluent toxicity in zebrafish (Danio rerio)
Smolders, R., Boeck, G. D., & Blust, R. (2003) · Environmental Toxicology and Chemistry · doi:10.1002/etc.5620220429 · Cited by 143
Ants affect the distribution and performance of seedlings of Clusia criuva, a primarily bird‐dispersed rain forest tree
Passos, L. C., & Oliveira, P. S. (2002) · Journal of Ecology · doi:10.1046/j.1365-2745.2002.00687.x · Cited by 143
Dietary α-linolenic acid is as effective as oleic acid and linoleic acid in lowering blood cholesterol in normolipidemic men
Chan, J., Bruce, V. M., & McDonald, B. (1991) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/53.5.1230 · Cited by 142
Human hepatic triglyceride lipase expression reduces high density lipoprotein and aortic cholesterol in cholesterol-fed transgenic mice.
Busch, S. J., Barnhart, R. L., Martin, G. A., Fitzgerald, M. C., Yates, M. T., Mao, S. J., Thomas, C. E., & Jackson, R. L. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34018-8 · Cited by 139
Quantitative seasonal changes in the protein, lipid and energy content of the carcass, ovaries and liver of adult female plaice, Pleuronectes platessa L.
Dawson, A. S., & Grimm, A. S. (1980) · Journal of Fish Biology · doi:10.1111/j.1095-8649.1980.tb03729.x · Cited by 143
Effect of (−)‐hydroxycitrate upon the accumulation of lipid in the rat: I. Lipogenesis
Sullivan, A. C., Triscari, J. M., Hamilton, J. G. C., Miller, O. N., & Wheatley, V. R. (1974) · Lipids · doi:10.1007/bf02532136 · Cited by 143
Extraction and separation of phosphate esters from plant tissues
Bieleski, R. L., & Young, R. E. (1963) · Analytical Biochemistry · doi:10.1016/0003-2697(63)90008-3 · Cited by 143
Phospholipid, protein, and nucleic acid increases in protoplasm and membrane structures associated with development of extreme freezing resistance in black locust tree cells
Siminovitch, D. J., Rheaume, B., Pomeroy, K., et al. (1968) · Cryobiology · doi:10.1016/s0011-2240(68)80164-6 · Cited by 143
Purification of synaptic vesicles from elasmobranch electric organ and the use of biophysical criteria to demonstrate purity
Carlson, S. S., Wagner, J. A., & Kelly, R. B. (1978) · Biochemistry · doi:10.1021/bi00600a009 · Cited by 143
A Method for the Rapid Isolation of Nuclear Membranes from Rat Liver
Kay, R. R., Fraser, D., & Johnston, I. R. (1972) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1972.tb02081.x · Cited by 143
The purification and characterization of CTP:phosphorylcholine cytidylyltransferase from rat liver.
Weinhold, P. A., Rounsifer, M. E., & Feldman, D. A. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)89220-7 · Cited by 143
Low concentrations of indomethacin inhibit phospholipase A2 of rabbit polymorphonuclear leukocytes.
Kaplan, L., Weiss, J. P., & Elsbach, P. (1978) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.75.6.2955 · Cited by 143
Characterization of an Estradiol-Induced Protein from Rainbow Trout Serum as Vitellogenin by the Composition and Radioimmunological Cross Reactivity to Ovarian Yolk Fractions1
Campbell, C. M., & Idler, D. R. (1980) · Biology of Reproduction · doi:10.1093/biolreprod/22.3.605 · Cited by 143
On the structure of the depot fats of marine fish and mammals
Brockerhoff, H., & Hoyle, R. J. (1963) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(63)90254-6 · Cited by 143
Fish Oil Supplementation of Control and (n-3) Fatty Acid-Deficient Male Rats Enhances Reference and Working Memory Performance and Increases Brain Regional Docosahexaenoic Acid Levels3
Chung, W.-L., Chen, J.-J., & Su, H. (2008) · Journal of Nutrition · doi:10.1093/jn/138.6.1165 · Cited by 144
High fructose feeding induces copper deficiency in Sprague–Dawley rats: A novel mechanism for obesity related fatty liver
Song, M., Schuschke, D. A., Zhou, Z., Chen, T., Pierce, W. M. J., Wang, R., Johnson, W. T., & McClain, C. J. (2011) · Journal of Hepatology · doi:10.1016/j.jhep.2011.05.030 · Cited by 143
The Perilipin Homologue, Lipid Storage Droplet 2, Regulates Sleep Homeostasis and Prevents Learning Impairments Following Sleep Loss
Thimgan, M. S., Suzuki, Y., Seugnet, L., Gottschalk, L., & Shaw, P. J. (2010) · PLoS Biology · doi:10.1371/journal.pbio.1000466 · Cited by 141
Separation of conjugated linoleic acid-containing mono-, di- and triacylglycerols by silver-ion high-performance liquid chromatography
Adlof, R. O. (2001) · European Journal of Lipid Science and Technology · doi:10.1002/1438-9312(200109)103:9 · Cited by 5
Optimum feeding rates for European sea bass Dicentrarchus labrax L. reared in seawater and freshwater
Eroldoğan, O. T., Kumlu, M., & Aktaş, M. (2003) · Aquaculture · doi:10.1016/j.aquaculture.2003.10.020 · Cited by 142
Algal cell rupture using high pressure homogenization as a prelude to oil extraction
Samarasinghe, N. U., Fernando, S., Lacey, R. E., & Faulkner, W. B. (2012) · Renewable Energy · doi:10.1016/j.renene.2012.04.039 · Cited by 142
From waste to energy: Microalgae production in wastewater and glycerol
Cabanelas, I. T. D., Arbib, Z., Chinalia, F. A., de Souza, C. O., Perales, J. A., de Almeida, P. F., Druzian, J. I., & Nascimento, I. A. (2013) · Applied Energy · doi:10.1016/j.apenergy.2013.04.023 · Cited by 142
A Role for Sphingomyelin-Rich Lipid Domains in the Accumulation of Phosphatidylinositol-4,5-Bisphosphate to the Cleavage Furrow during Cytokinesis
Abe, M., Makino, A., Hullin‐Matsuda, F., et al. (2012) · Molecular and Cellular Biology · doi:10.1128/mcb.06113-11 · Cited by 142
Characterization of bio-oil and its sub-fractions from pyrolysis of Scenedesmus dimorphus
Bordoloi, N., Narzari, R., Sut, D., Saikia, R., Chutia, R. S., & Kataki, R. (2016) · Renewable Energy · doi:10.1016/j.renene.2016.03.081 · Cited by 140
Encapsulation of fish oil in nanofibers by emulsion electrospinning: Physical characterization and oxidative stability
García‐Moreno, P. J., Stephansen, K., van der Kruijs, J., et al. (2016) · Journal of Food Engineering · doi:10.1016/j.jfoodeng.2016.03.015 · Cited by 143
High-resolution NMR studies of antibiotics in cellular membranes
Medeiros‐Silva, J., Jekhmane, S., Paioni, A. L., Gawarecka, K., Baldus, M., Świeżewska, E., Breukink, E. J., & Weingarth, M. H. (2018) · Nature Communications · doi:10.1038/s41467-018-06314-x · Cited by 142
Panax notoginseng: derived exosome-like nanoparticles attenuate ischemia reperfusion injury via altering microglia polarization
Li, S., Zhang, R., Wang, A., Li, Y., Zhang, M., Kim, J., Zhu, Y., Wang, Q., Zhang, Y., Wei, Y., et al. (2023) · Journal of Nanobiotechnology · doi:10.1186/s12951-023-02161-1 · Cited by 154
“The proteins and lipids of PDNs were extracted from PDN samples with RIPA protein isolation kit by Bligh and Dyer method [38], respectively.”
Method
Cryopreservation of Mouse Spermatozoa
Songsasen, N., & Leibo, S. P. (1997) · Cryobiology · doi:10.1006/cryo.1997.2047 · Cited by 142
Overexpression, purification and characterization of human recombinant 15-lipoxygenase
Kühn, H., Barnett, J. W., Grunberger, D., et al. (1993) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(93)90085-n · Cited by 142
Improvement of a solid phase extraction method for analysis of lipid fractions in muscle foods
Ruiz, J., Antequera, T., Andrés, A. I., et al. (2004) · Analytica Chimica Acta · doi:10.1016/j.aca.2004.04.059 · Cited by 142
The gross and amino acid compositions of batch and semi-continuous cultures ofIsochrysis sp. (clone T.ISO),Pavlova lutheri andNannochloropsis oculata
Brown, M. R., Garland, C. D., Jeffrey, S. W., Jameson, I. D., & LeRoi, J. (1993) · Journal of Applied Phycology · doi:10.1007/bf02186231 · Cited by 142
Diosgenin, the Main Aglycon of Fenugreek, Inhibits LXRα Activity in HepG2 Cells and Decreases Plasma and Hepatic Triglycerides in Obese Diabetic Mice
Uemura, T., Goto, T., Kang, M., Mizoguchi, N., Hirai, S., Lee, J.-Y., Nakano, Y., Shono, J., Hoshino, S., Taketani, K., et al. (2010) · Journal of Nutrition · doi:10.3945/jn.110.125591 · Cited by 143
A Lipid-Specific Toxin Reveals Heterogeneity of Sphingomyelin-Containing Membranes
Ishitsuka, R., Yamaji‐Hasegawa, A., Makino, A., et al. (2004) · Biophysical Journal · doi:10.1016/s0006-3495(04)74105-3 · Cited by 142
Membrane Translocation of 15-Lipoxygenase in Hematopoietic Cells Is Calcium-Dependent and Activates the Oxygenase Activity of the Enzyme
Brinckmann, R., Schnurr, K., Heydeck, D., Rosenbach, T., Kolde, G., & Kühn, H. (1998) · Blood · doi:10.1182/blood.v91.1.64 · Cited by 146
Purslane: A Terrestrial Source of Omega-3 Fatty Acids
Simopoulos, A. P., & Salem, N. (1986) · New England Journal of Medicine · doi:10.1056/nejm198609253151313 · Cited by 141
Effect of Energy Level in Lactating Sows on Yield and Composition of Milk and Nutrient Balance of Piglets
Noblet, J., & Etienne, M. L. (1986) · Journal of Animal Science · doi:10.2527/jas1986.6361888x · Cited by 142
Protein kinase C is increased in the liver of humans and rats with non-insulin-dependent diabetes mellitus: an alteration not due to hyperglycemia.
Considine, R. V., Nyce, M. R., Allen, L. E., Morales, L. M., Triester, S., Serrano, J., Colberg, J., Lanza-Jacoby, S., & Caro, J. F. (1995) · Journal of Clinical Investigation · doi:10.1172/jci118001 · Cited by 141
Thiobarbituric acid‐reactive substances from peroxidized lipids
Kosugi, H., Kojima, T., & Kikugawa, K. (1989) · Lipids · doi:10.1007/bf02535762 · Cited by 142
Packing of cholesterol molecules in human low-density lipoprotein
Lund‐Katz, S., & Phillips, M. C. (1986) · Biochemistry · doi:10.1021/bi00355a016 · Cited by 141
Separate transport systems for biliary secretion of sulfated and unsulfated bile acids in the rat.
Kuipers, F., Enserink, M., Havinga, R., et al. (1988) · Journal of Clinical Investigation · doi:10.1172/jci113493 · Cited by 142
Stimulation of Phospholipase D by Epidermal Growth Factor Requires Protein Kinase C Activation in Swiss 3T3 Cells
Yeo, E., & Exton, J. H. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.8.3980 · Cited by 142
A membrane-bound activity catalysing phosphatidylinositol breakdown to 1,2-diacylglycerol, d -myoinositol 1:2-cyclic phosphate and d -myoinositol 1-phosphate. Properties and subcellular distribution in rat cerebral cortex
Lapetina, E. G., & Michell, R. H. (1973) · Biochemical Journal · doi:10.1042/bj1310433 · Cited by 142
Penetration of Lipid Monolayers by Polyene Antibiotics
DEMEL, R. A., Deenen, L. L. M. V., & Kinsky, S. C. (1965) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)97388-6 · Cited by 142
Green technology for the industrial production of biofuels and bioproducts from microalgae: A review
Tang, D. Y. Y., Yew, G. Y., Koyande, A. K., Chew, K. W., Vo, D.-V. N., & Show, P. L. (2020) · Environmental Chemistry Letters · doi:10.1007/s10311-020-01052-3 · Cited by 142
Bicarbonate supplementation enhanced biofuel production potential as well as nutritional stress mitigation in the microalgae Scenedesmus sp. CCNM 1077
Pancha, I., Chokshi, K., Ghosh, T., Paliwal, C., Maurya, R., & Mishra, S. (2015) · Bioresource Technology · doi:10.1016/j.biortech.2015.06.107 · Cited by 142
Direct determination of phospholipid structures in microorganisms by fast atom bombardment triple quadrupole mass spectrometry
Cole, M. J., & Enke, C. G. (1991) · Analytical Chemistry · doi:10.1021/ac00010a020 · Cited by 141
Recent developments in sample-pretreatment techniques for mass spectrometry-based metabolomics
Raterink, R.-J., Lindenburg, P. W., Vreeken, R. J., Ramautar, R., & Hankemeier, T. (2014) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2014.06.003 · Cited by 141
Effects of different alcoholic extraction conditions on soybean oil yield, fatty acid composition and protein solubility of defatted meal
Sawada, M. M., Venâncio, L. L., Toda, T. A., & Rodrigues, C. E. D. C. (2014) · Food Research International · doi:10.1016/j.foodres.2014.04.039 · Cited by 141
Fibroblast Growth Factor 21 Improves Insulin Resistance and Ameliorates Renal Injury in db/db Mice
Kim, H. W., Lee, J. E., Cha, J. J., Hyun, Y. Y., Kim, J. E., Lee, M. H., Song, H. K., Nam, D. H., Han, J., Han, S. Y., et al. (2013) · Endocrinology · doi:10.1210/en.2012-2276 · Cited by 142
Supercritical Carbon Dioxide Extraction of Algal Lipids for the Biodiesel Production
Santana, Á. L., Jesus, S. L. F. D., Larrayoz, M. A., & Filho, R. M. (2012) · Procedia Engineering · doi:10.1016/j.proeng.2012.07.569 · Cited by 143
Evolutionary engineered Saccharomyces cerevisiae wine yeast strains with increased in vivo flux through the pentose phosphate pathway
Cadière, A., Ortiz-Julien, A., Camarasa, C., & Dequin, S. (2011) · Metabolic Engineering · doi:10.1016/j.ymben.2011.01.008 · Cited by 141
Choline intake and genetic polymorphisms influence choline metabolite concentrations in human breast milk and plasma
Fischer, L. M., Costa, K. A. D., Galanko, J. A., Sha, W., Stephenson, B., Vick, J. A., & Zeisel, S. H. (2010) · American Journal of Clinical Nutrition · doi:10.3945/ajcn.2010.29459 · Cited by 141
Activation of Calcium/Calmodulin-Dependent Protein Kinase II in Obesity Mediates Suppression of Hepatic Insulin Signaling
Özcan, L., de Souza, J. C., Harari, A. A., Backs, J., Olson, E. N., & Tabas, I. A. (2013) · Cell Metabolism · doi:10.1016/j.cmet.2013.10.011 · Cited by 145
High intensity interval training improves liver and adipose tissue insulin sensitivity
Marcinko, K., Sikkema, S. R., Samaan, M. C., Kemp, B. E., Fullerton, M. D., & Steinberg, G. R. (2015) · Molecular Metabolism · doi:10.1016/j.molmet.2015.09.006 · Cited by 146
Oleaginous yeast Yarrowia lipolytica culture with synthetic and food waste-derived volatile fatty acids for lipid production
Gao, R., Li, Z., Zhou, X., et al. (2017) · Biotechnology for Biofuels · doi:10.1186/s13068-017-0942-6 · Cited by 141
Global landscape of cell envelope protein complexes in Escherichia coli
Babu, M., Bundalovic-Torma, C., Calmettes, C., Phanse, S., Zhang, Q., Jiang, Y., Minić, Z., Kim, S., Mehla, J., Gagarinova, A. G., et al. (2017) · Nature Biotechnology · doi:10.1038/nbt.4024 · Cited by 130
“Lipidomics”: Mass spectrometric and chemometric analyses of lipids
Wu, Z., Bagarolo, G. I., Thoröe‐Boveleth, S., & Jankowski, J. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.06.009 · Cited by 148
Metabolic Fingerprinting Links Oncogenic PIK3CA with Enhanced Arachidonic Acid-Derived Eicosanoids
Koundouros, N., Karali, E., Tripp, A., Valle, A., Inglese, P., Perry, N. J. S., Magee, D. J., Virmouni, S. A., Elder, G. A., Tyson, A. L., et al. (2020) · Cell · doi:10.1016/j.cell.2020.05.053 · Cited by 144
“Lipid extraction and eicosanoid profiling Total lipids were extracted based on the Bligh and Dyer method (Bligh and Dyer, 1959) and analyzed as described previously (Wolfer et al.”
Method
Patterns of variation in the lipid class and fatty acid composition of Nannochloropsis oculata (Eustigmatophyceae) during batch culture
Hodgson, P. A., Henderson, R. J., SARGENT, J. R., & Leftley, J. W. (1991) · Journal of Applied Phycology · doi:10.1007/bf00003699 · Cited by 140
Biosynthesis of endotoxins. Purification and catalytic properties of 3-deoxy-D-manno-octulosonic acid transferase from Escherichia coli.
Belunis, C. J., & Raetz, C. R. H. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)50189-2 · Cited by 141
Properties of spermatozoal superoxide dismutase and lack of involvement of superoxides in metal-ion-catalysed lipid-peroxidation and reactions in semen
Mennella, M. R. F., & Jones, R. (1980) · Biochemical Journal · doi:10.1042/bj1910289 · Cited by 140
Antineoplastic Agents. 520. Isolation and Structure of Irciniastatins A and B from the Indo-Pacific Marine Sponge Ircinia ramosa
Pettit, G. R., Xu, J., Chapuis, J., et al. (2004) · Journal of Medicinal Chemistry · doi:10.1021/jm030207d · Cited by 141
TOTAL AND ORGANIC PHOSPHORUS IN CHILEAN VOLCANIC SOILS
Borie, F., & Rubio, R. (2003) · Gayana. Botánica · doi:10.4067/s0717-66432003000100011 · Cited by 141
The impact of grassland management regime on the community structure of selected bacterial groups in soils
Clegg, C. D., Lovell, R. D., & Hobbs, P. J. (2003) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.2003.tb01066.x · Cited by 141
Effect of temperature on the fatty acid composition and temporal trajectories of fatty acids in fasting Daphnia pulex (Crustacea, cladocera)
Schlechtriem, C., Arts, M. T., & Zellmer, I. D. (2006) · Lipids · doi:10.1007/s11745-006-5111-9 · Cited by 141
Long-chain polyunsaturated fatty acids at birth and cognitive function at 7 y of age
Bakker, E. C., Ghys, A. J. A., Kester, A. D. M., Vles, J. S. H., Dubas, J. S., Blanco, C. E., & Hornstra, G. (2003) · European Journal of Clinical Nutrition · doi:10.1038/sj.ejcn.1601506 · Cited by 141
Calcitonin is a major regulator for the expression of renal 25-hydroxyvitamin D 3 -1α-hydroxylase gene in normocalcemic rats
Shinki, T., Ueno, Y., DeLuca, H. F., & Suda, T. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.14.8253 · Cited by 141
Lipid traffic between high density lipoproteins and Plasmodium falciparum-infected red blood cells.
Grellier, P., Rigomier, D., Barthelemy-Clavey, V., Fruchart, J., & Schrével, J. (1991) · The Journal of Cell Biology · doi:10.1083/jcb.112.2.267 · Cited by 141
Vegetable Oils High in Phytosterols Make Erythrocytes Less Deformable and Shorten the Life Span of Stroke-Prone Spontaneously Hypertensive Rats
Ratnayake, W. M. N., L’Abbé, M. R., Mueller, R., Hayward, S., Plouffe, L. A., Hollywood, R., & Trick, K. D. (2000) · Journal of Nutrition · doi:10.1093/jn/130.5.1166 · Cited by 141
A cytosolic phospholipase in human neutrophils that hydrolyzes arachidonoyl-containing phosphatidylcholine
Alonso, F., Henson, P. M., & Leslie, C. C. (1986) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(86)90156-6 · Cited by 141
Origins and fates of fatty acyl-CoA esters
Waku, K. (1992) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(92)90085-a · Cited by 141
Differences in postprandial lipemia between patients with normal glucose tolerance and noninsulin-dependent diabetes mellitus
Chen, Y. I., Swami, S., Skowroński, R., Coulston, A. M., & Reaven, G. M. (1993) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.76.1.8421086 · Cited by 143
Studies on Calciferol Metabolism
Henry, H. L., & Norman, A. W. (1974) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)81270-x · Cited by 141
Evidence for the role of growth hormone as a part of a 'light–pituitary axis' in growth and smoltification of Atlantic salmon ( Salmo salar )
Komourdjian, M. P., Saunders, R. L., & Fenwick, J. C. (1976) · Canadian Journal of Zoology · doi:10.1139/z76-062 · Cited by 141
Accumulation and metabolism of carbon-14 labeled benzene, naphthalene, and anthracene by young coho salmon (Oncorhynchus kisutch)
Roubal, W. T., Collier, T. K., & Malins, D. C. (1977) · Archives of Environmental Contamination and Toxicology · doi:10.1007/bf02220929 · Cited by 141
Epoxides as products of lipid autoxidation in rat lungs
Sevanian, A., Mead, J. F., & Stein, R. A. (1979) · Lipids · doi:10.1007/bf02533449 · Cited by 141

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks