Evolutionary engineered Saccharomyces cerevisiae wine yeast strains with increased in vivo flux through the pentose phosphate pathway
Abstract
Amplification of the flux toward the pentose phosphate (PP) pathway might be of interest for various S. cerevisiae based industrial applications. We report an evolutionary engineering strategy based on a long-term batch culture on gluconate, a substrate that is poorly assimilated by S. cerevisiae cells and is metabolized by the PP pathway. After adaptation for various periods of time, we selected strains that had evolved a greater consumption capacity for gluconate. (13)C metabolic flux analysis on glucose revealed a redirection of carbon flux from glycolysis towards the PP pathway and a greater synthesis of lipids. The relative flux into the PP pathway was 17% for the evolved strain (ECA5) versus 11% for the parental strain (EC1118). During wine fermentation, the evolved strains displayed major metabolic changes, such as lower levels of acetate production, higher fermentation rates and enhanced production of aroma compounds. These represent a combination of novel traits, which are of great interest in the context of modern winemaking.
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Experiments on pentose utilization highlighted that increased gene copy numbers of the heterologous genes in recombinant S. cerevisiae can emerge during evolutionary engineering and contribute to increased growth in anaerobic conditions.
“Furthermore, experiments on pentose utilization highlighted that increased gene copy numbers of the heterologous genes in recombinant S. cerevisiae can emerge during evolutionary engineering and contribute to increased growth in anaerobic conditions [104,109].”
From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [109]
Several studies improved the carbon source utilization of S. cerevisiae strains by genetic engineering in combination with evolutionary adaptation.
“For example, several studies improved the carbon source utilization of S. cerevisiae strains by genetic engineering in combination with evolutionary adaptation [104,107-109].”
From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [109]
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- Authors
- Cadière, A., Ortiz-Julien, A., Camarasa, C., & Dequin, S.
- Affiliations
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Lallemand (France) (FR)
- Corresponding author
- Dequin, Sylvie
- Venue
- Metabolic Engineering
- Publisher
- Elsevier BV
- Published
- 2011-05
- Volume
- 13
- Issue
- 3
- Pages
- 263-271
- ISSN
- 1096-7176 (ISSN-L)
- OpenAlex
- W2058737346
- Cited by
- 141
- References
- 59
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 12 · 2023: 7 · 2022: 3 · 2021: 8 · 2020: 9 · 2019: 8 · 2018: 9 · 2017: 17 · 2016: 10 · 2015: 9 · 2014: 18 · 2013: 7 · 2012: 8
- FWCI
- 4.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Metabolic Engineering (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Agence Nationale de la Recherche (ANR-05-PNRA-0011)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Saccharomyces cerevisiae, wine yeast, pentose phosphate pathway, evolutionary engineering, metabolic flux redirection, lipogenesis, acetate production, aroma production, fermentation rate, 13C metabolic flux analysis, industrial yeast strains
- MeSH terms
- Gluconates, Glucose, Glycolysis, Pentose Phosphate Pathway, Saccharomyces cerevisiae, Wine, Directed Molecular Evolution
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