Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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Method
Inclusion levels of flour made from smoked Nile tilapia trimmings in extruded corn snacks
de Souza, M. L. R., da Silva, D. D. S., Pereira, I. L., et al. (2021) · Research Society and Development · doi:10.33448/rsd-v10i8.17243 · Cited by 7
“The total lipid content was determined using the Bligh & Dyer (1959) methodology.”
Method
Zobellia roscoffensis sp. nov. and Zobellia nedashkovskayae sp. nov., two flavobacteria from the epiphytic microbiota of the brown alga Ascophyllum nodosum, and emended description of the genus Zobellia
Barbeyron, T., Thiébaud, M., Duff, N. L., et al. (2021) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.004913 · Cited by 7
Camelina sativa phosphatidylcholine:diacylglycerol cholinephosphotransferase‐catalyzed interconversion does not discriminate between substrates
Demski, K., Jeppson, S., Stymne, S., et al. (2021) · Lipids · doi:10.1002/lipd.12322 · Cited by 7
Viral Infection Drives the Regulation of Feeding Behavior Related Genes in Salmo salar
Muñoz, D., Fuentes, R., Carnicero, B., Aguilar, A., Sanhueza, N., Martin, S. S., Agurto-Muñoz, C., Donoso, A., Valdivia, L. E., Míguez, J. M., et al. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms222111391 · Cited by 7
“Lipids were extracted from liver (three fish per time and treatment; n = 18 total) as described by Bligh and Dyer [74].”
Method
The phospho-base N-methyltransferases PMT1 and PMT2 produce phosphocholine for leaf growth in phosphorus-starved Arabidopsis
Ngo, A. H., Angkawijaya, A. E., Lin, Y., et al. (2021) · Journal of Experimental Botany · doi:10.1093/jxb/erab436 · Cited by 7
Evaluation and characterization of a new method of extracting bark wax from Pinus radiata D. Don
Sandoval-Rivas, D., Moczko, E., Morales, D. V., et al. (2021) · Industrial Crops and Products · doi:10.1016/j.indcrop.2021.114161 · Cited by 7
Nutritional quality and sensory acceptance of biofortified cassava
Tagliapietra, B. L., Zanon, A. J., Tironi, L. F., et al. (2021) · Brazilian journal of food technology/Brazilian Journal of Food Technology · doi:10.1590/1981-6723.24720 · Cited by 7
A comparison of food sources of nudibranch mollusks at different depths off the Kuril Islands using fatty acid trophic markers
Komisarenko, A., Mordukhovich, V. V., Александровна, Е. И., & Imbs, A. B. (2021) · PeerJ · doi:10.7717/peerj.12336 · Cited by 7
Molecular Weight Analysis of Blue Shark (Prionace glauca) Collagen Hydrolysates by GPC-LS; Effect of High Molecular Weight Hydrolysates on Fibroblast Cultures: mRNA Collagen Type I Expression and Synthesis
Blanco, M., Sanz, N., Sánzhez, A. C., et al. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms23010032 · Cited by 7
“Fat content was determined by the Bligh and Dyer method [22].”
Method
Evaluation of physicochemical, bioactive composition and profile of fatty acids in leaves of different olive cultivars
Antunes, B. D. F., Otero, D. M., Bonemann, D. H., Ribeiro, A. S., Jacques, A. C., & Zambiazi, R. C. (2021) · Revista CERES · doi:10.1590/0034-737x202168060002 · Cited by 8
Novel Extraction Method for Combined Lipid and Metal Speciation From Caenorhabditis elegans With Focus on Iron Redox Status and Lipid Profiling
Blume, B., Witting, M. A., Schmitt‐Kopplin, P., & Michalke, B. (2021) · Frontiers in Chemistry · doi:10.3389/fchem.2021.788094 · Cited by 7
“Yields and recoveries were similar to the lipid extraction protocols by Bligh and Dyer (Bligh and Dyer 1959) and Folch (Folch, Lees et al.”
Result
Immune-modulation effect of Halocynthia aurantium tunic lipid on RAW264.7 cells
Jang, A.-Y., Monmai, C., Rod–in, W., Kim, J.-E., You, S., Lee, T., & Park, W. J. (2021) · Food Science and Biotechnology · doi:10.1007/s10068-021-01017-4 · Cited by 6
“Their tunic was separated and collected and the lipid was extracted according to the modified method of Bligh and Dyer (1959).”
Method
Effects of Dietary Incorporation of Grape Stalks Untreated and Fungi-Treated in Growing Rabbits: A Preliminary Study
da Silva, V. M. A., Pinheiro, V. M. C., Alves, A., et al. (2022) · Animals · doi:10.3390/ani12010112 · Cited by 7
“The fatty acids were obtained using the protocol described by Bligh and Dyer [33] with modifications proposed by Barros [34].”
Method
Investigation of Pineapple Remnants Used in Fish Aqua Feeds
al., R. F. A. E. (2022) · Egyptian Journal of Aquatic Biology and Fisheries · doi:10.21608/ejabf.2022.213363 · Cited by 7
Solid-State Fermentation: An Alternative Approach to Produce Fungal Lipids as Biodiesel Feedstock
Khot, M. B. (2022) · Clean Energy Production Technologies · doi:10.1007/978-981-16-4509-9_6 · Cited by 7
Lymphatic Absorption of Microbial Plasmalogens in Rats
Sato, N., Kanehama, A., Kashiwagi, A., Yamada, M., & Nishimukai, M. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2022.836186 · Cited by 7
“Analyses Total lipids in the lymphatic fluid were extracted by the Bligh & Dyer method (Bligh and Dyer, 1959).”
Method
Quantitative Lipidomic Analysis of Takotsubo Syndrome Patients' Serum
Karnati, S., Güntaş, G., Rajendran, R., Shityakov, S., Höring, M., Liebisch, G., Kosanovic, D., Ergün, S., Nagai, M., & Förster, C. Y. (2022) · Frontiers in Cardiovascular Medicine · doi:10.3389/fcvm.2022.797154 · Cited by 6
“Serum samples were quantified and 5 μl of each subject were used for extraction, and lipids were extracted according to the procedure described by Bligh and Dyer (20).”
Method
Pantanal yacare ( Caiman yacare ) tail fillets subjected to traditional hot smoking and liquid smoke
de Souza, M. L. R., Fernandes, V. R. T., Gasparino, E., et al. (2022) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.12009 · Cited by 7
Aquirufa lenticrescens sp. nov. and Aquirufa aurantiipilula sp. nov.: two new species of a lineage of widespread freshwater bacteria
Pitt, A., Koll, U., Schmidt, J., et al. (2022) · Archives of Microbiology · doi:10.1007/s00203-022-02950-6 · Cited by 7
Characterization of novel euryhaline microalgal cultures from Punjab, India for bioactive compounds
Garg, D., Dar, R. A., & Phutela, U. G. (2022) · Archives of Microbiology · doi:10.1007/s00203-022-03002-9 · Cited by 8
“The lipid content was estimated using Bligh and Dyer (1959) technique.”
Method
Heterologous Expression and Characterization of Plant Wax Ester Producing Enzymes
Cheng, D., Li, L., Rizhsky, L., et al. (2022) · Metabolites · doi:10.3390/metabo12070577 · Cited by 7
“The yeast lipids were extracted using a modification of a method described previously [66].”
Method

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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