Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

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Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

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BANCROFT W., 1895, Phys. Iiev., 3, 1

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“…extraction was performed according to the method of Bligh and Dyer in the presence of not naturally occurring lipid species as internal standards (59): PC 14:0/14:0, PC 22:0/22:0, PE 14:0/14:0, PE 20:0/20:0 (di-phytanoyl), PS 14:0/14:0, PS 20:0/20:0 (di-phytanoyl), PI 17:0/17:0, LPC 13:0, LPC…”
Method
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“The numerical values of TBARs are expressed in mg MDA/kg. Agnieszka’s method was used to determine the acid values [21].”
Method
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“Lipid extracts were transmethylated in a hot water bath at 65 ℃ for 60 min with 0.5 mL of MeOH, benzene, and acetyl chloride (20:4:1 v/v) [20].”
Influential
Rhodococcus navarretei sp. nov. and Pseudarthrobacter quantipunctorum sp. nov., two novel species with the ability to biosynthesize fluorescent nanoparticles, isolated from soil samples at Union Glacier, Antarctica
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“For fatty acid extraction, strains EXRC- 4A-4 T and RC- 2-3 T were grown on TSA medium and a loopful of culture was used for extraction through methylation and liquid extraction of their fatty acid methyl esters (FAMEs) [31, 32].”
Metabolite changes by combined treatment, ethyl formate and low temperature, in Drosophila suzukii
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“Total lipids were extracted from the whole bodies of D. suzukii pupae in triplicate (100 insects/replicate) by the modified Bligh and Dyer method as described in a previous study 17,20 .”
Method
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McIntyre, J. C., Watson, D., & Sleight, R. G. (1993) · Chemistry and Physics of Lipids · doi:10.1016/0009-3084(93)90002-k · Cited by 7
Methyl esters from the copepod Tisbe biminiensis assayed by two transesterification methods
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Hayashi, K., & BOWER, J. R. F. (2004) · Journal of Oleo Science · doi:10.5650/jos.53.1 · Cited by 7
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Synthesis of lipids for development of multifunctional lipid-based drug-carriers
Zhu, G., Alhamhoom, Y., Cummings, B. S., et al. (2011) · Bioorganic & Medicinal Chemistry Letters · doi:10.1016/j.bmcl.2011.08.103 · Cited by 7
The heptadecanoic fatty aldehyde—one of the main aldehydes of the far-eastern Bryozoa
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Influence of glucocorticoids, neuregulin-1β, and sex on surfactant phospholipid secretion from type II cells
King, G., Damas, J. E., Cake, M. H., et al. (2013) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00297.2013 · Cited by 7
Electrospray ionization–tandem mass spectrometry analysis of phospholipid molecular species from Antarctic and non-Antarctic yeasts
Bhuiyan, M., Tucker, D. J., & Watson, K. (2014) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2014.07.011 · Cited by 7
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The interaction of aurein 2.5 with fungal membranes
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Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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