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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Abstract
Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.
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“in addition to gravimetry measurements [14,15], a large panel of chromatography strategies have been developed, like thin-layer chromatography (TLC), high-pressure liquid chromatography (HPLC) and gas chromatography (GC) coupled with mass spectrometry (MS) [16].”
From The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae (2015) · cites this paper as [14]
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Metadata
- Authors
- Bligh, E. G., & Dyer, W. J.
- Venue
- Canadian Journal of Biochemistry and Physiology
- Publisher
- Canadian Science Publishing
- Published
- 1959-08-01
- Volume
- 37
- Issue
- 8
- Pages
- 911-917
- ISSN
- 0576-5544 (ISSN-L)
- OpenAlex
- W2152311269
- Cited by
- 54,751
- References
- 5
- Citations by year
- 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
- FWCI
- 8.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Canadian Journal of Biochemistry and Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
- Keywords
- lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
- MeSH terms
- Fishes, Lipids
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