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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Abstract
Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.
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“in addition to gravimetry measurements [14,15], a large panel of chromatography strategies have been developed, like thin-layer chromatography (TLC), high-pressure liquid chromatography (HPLC) and gas chromatography (GC) coupled with mass spectrometry (MS) [16].”
From The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae (2015) · cites this paper as [14]
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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citationBonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18
Research works printed but not linked: 1
- BANCROFT W., 1895, Phys. Iiev., 3, 1
Cited by
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Items 46,001–46,100 of 48,847
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Shin, K.-H., Nagamori, T., Kimura, Y., et al. (1995) · Chemistry and Physics of Lipids · doi:10.1016/0009-3084(94)02435-8
von Herrath, D., Kraft, D., & Schaefer, K. (1973) · Naunyn-Schmiedeberg s Archives of Pharmacology · doi:10.1007/bf00501486
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Lappeteläinen, R., Savolainen, K., & Mäenpää, P. H. (1984) · Biochemical Pharmacology · doi:10.1016/0006-2952(84)90429-5
Charakterisierung der renalen 25‐Hydroxycholecalciferol‐1‐Hydroxylase beim Schwein
Schreiner, F., & Harmeyer, J. (1986) · Zentralblatt für Veterinärmedizin Reihe A · doi:10.1111/j.1439-0442.1986.tb00587.x
Alveolar phospholipids, hyperventilation and temperature of conservation of rat lungs
Brondel, L., RAMI, J., Rivière, D., et al. (1989) · Respiration Physiology · doi:10.1016/0034-5687(89)90022-4
Dhall, K., Majumdar, S., Narang, A., et al. (1986) · Asia-Oceania Journal of Obstetrics and Gynaecology · doi:10.1111/j.1447-0756.1986.tb00210.x
Rai, K. P., Gendley, M. K., Tiwari, S. P., et al. (2015) · Veterinary World · doi:10.14202/vetworld.2015.187-191
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Yan, H., Jia, X., He, H., et al. (2011) · International Conference on Computer Distributed Control and Intelligent Environmental Monitoring · doi:10.1109/cdciem.2011.237
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Hong, Y.-K., Kim, J., & Ng, K. Y. S. (2011) · Journal of the Korean Solar Energy Society · doi:10.7836/kses.2011.31.6.066
Reis, C., & Netto, F. M. (2006) · Boletim do Centro de Pesquisa de Processamento de Alimentos · doi:10.5380/cep.v24i2.7492
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Liu, F., Cao, G., Guan, Z.-B., et al. (2014) · Journal of the American Oil Chemists Society · doi:10.1007/s11746-014-2576-1
Structural changes in the cell membrane of λ-lysogenic Escherichia coli induced by colicin E2
Yamamoto, H., Beppu, T., & Arima, Κ. (1977) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(77)91628-x
Manrique, M. R. G., Lostao, Y., Antolín, G., et al. (2006) · doi:10.1051/iufost:20060085
Xu, Y., & Siegenthaler, P. (1995) · Springer eBooks · doi:10.1007/978-94-015-8394-7_50
Inokuchi, H., Mizuno‐Kamiya, M., & Fujita, A. (2001) · Japanese Journal of Oral Biology · doi:10.2330/joralbiosci1965.43.666
Truax, J. F., & Cochran, F. R. (1995) · Inflammation Research · doi:10.1007/bf01778299
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Shieh, H. S. (1969) · Canadian Journal of Microbiology · doi:10.1139/m69-055
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Marengo, B., Bottini, C., Porta, C. A. M. L., et al. (2006) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2006.05.101
Boucrot, P., Bobin‐Dubigeon, C., Elkihel, L., et al. (1998) · Fundamental and Clinical Pharmacology · doi:10.1111/j.1472-8206.1998.tb00968.x
El-Nady, F. E. (1996) · Journal of Environmental Science and Health Part A Environmental Science and Engineering and Toxicology · doi:10.1080/10934529609376517
Feeding Effect of Some Poultry Byproducts on Chicken's Serum Fatty Acids
Farag, R. S., Mostafa, M. A. F., Abdella, A. F., et al. (1984) · Fette Seifen Anstrichmittel · doi:10.1002/lipi.19840860108
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Sartori, T. C. F. D. T., Brazaca, S. G. C., dos Santos, M. A. T., et al. (2014) · Boletim do Centro de Pesquisa de Processamento de Alimentos · doi:10.5380/cep.v32i2.39078
Mechanism of inactivation of hepatitis B surface antigen by N alpha-cocoyl-L-arginine ethyl ester
Sugimoto, Y., & Toyoshima, S. (1981) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.20.1.120
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Weber, N., & Richter, K. ‐. (1995) · Fette Seifen Anstrichmittel · doi:10.1002/lipi.19950970506
Labelled lipids distribution following [3H]glycerol injection to pregnant rabbits
Acebal, C., Arche, R., Castro, J., et al. (1983) · International Journal of Biochemistry · doi:10.1016/0020-711x(83)90185-4
The biological fouling of cartridge filters in aqueous applications
Williams, C. J., & Edyvean, R. G. J. (1995) · Biofouling · doi:10.1080/08927019509378296
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Naeem, M., Salam, A., Ali, M., et al. (2010) · doi:10.1109/iccceng.2010.5560387
Wang, S.-F., Feng, Y.-M., Feng, Z., et al. (1989) · Journal of Tongji Medical University · doi:10.1007/bf02933744
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Erkomaa, K., Haapala, K., Järvinen, O., et al. (1997) · Marine Pollution Bulletin · doi:10.1016/s0025-326x(97)00164-1
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Pühringer, I. (2011) · University of Vienna · doi:10.25365/thesis.14582
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Savolainen, J. E. T., Vuori, A. T., & Gyllenberg, H. G. (1969) · Acta Agriculturae Scandinavica · doi:10.1080/00015126909433189
Gómez-Rodríguez, M. V., del Castillo, J. D. D. L., & Alvarez‐Tinaut, M. C. (1987) · Journal of Plant Nutrition · doi:10.1080/01904168709363774
Peroxisomal Proliferation in Rat Liver and Essential Fatty Acid Deficiency
Rabetafika, E., Carreau, J. P., & Goascogne, C. L. (2002) · Journal of Clinical Biochemistry and Nutrition · doi:10.3164/jcbn.31.27
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OSORIO, F. A., Crisostomo, J., Baeza, S., et al. (2011) · Procedia Food Science · doi:10.1016/j.profoo.2011.09.047
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Kim, H. J., Yoon, M.-S., Park, Y.-S., et al. (2008) · Journal of the Korean Society of Food Science and Nutrition · doi:10.3746/jkfn.2008.37.11.1457
Oyarzún, M. J., Morgado, E., Nelson Dussaubat, D., Lathrop, M. E., Iturriaga, R., Larrain, C., & Zapata, P. (1992) · Comparative Biochemistry and Physiology Part A Physiology · doi:10.1016/0300-9629(92)90720-b
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Menzies, A. R., & Edwards, H. E. (1987) · International Journal of Pharmaceutics · doi:10.1016/0378-5173(87)90010-x
Che, R., Wang, Q. M., Huang, L., Zhao, P., & Yu, X. Y. (2013) · Advanced materials research · doi:10.4028/www.scientific.net/amr.860-863.920
Reutergardh, L. B. (1996) · Resources Conservation and Recycling · doi:10.1016/0921-3449(95)00067-4
Si, Y., Huizhu, X., Xiong, Z., et al. (1996) · Journal of Tongji Medical University · doi:10.1007/bf02908805
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Hadjiivanova, N., Koumanov, K. S., Panajotov, I., et al. (1984) · International Journal of Biochemistry · doi:10.1016/0020-711x(84)90072-7
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Isai, S. V., Иванкина, Н. Ф., Kafanova, T. V., et al. (1994) · Pharmaceutical Chemistry Journal · doi:10.1007/bf02219253
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Skúladóttir, G. V., Olason, P. I., Jónsson, L., Skarphedinsson, J. O., Eriksdottir, V. H., Atlason, P. T., Franzson, L., & Schiöth, H. B. (2003) · Lipids · doi:10.1007/s11745-003-1170-1
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Bushway, A. A., Work, T., Bayer, R. C., et al. (2001) · Journal of Aquatic Food Product Technology · doi:10.1300/j030v10n02_05
Montanher, P. F., de Paula, L. N., Coró, F. A. G., et al. (2012) · Food and public health · doi:10.5923/j.fph.20120205.06
Proulx, P. R., & Aubry, H. (1990) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(90)90082-9
Duarte, I. C. N., Matias, L. G. O., Souza, L. D., Pinto, C. H. C., Rebouças, R. F. D. C., & Gomes, A. F. (2015) · Revista Gestão Inovação e Tecnologias · doi:10.7198/s2237-0722201500010013
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Wettasinghe, M., & Shahidi, F. (1998) · Journal of Texture Studies · doi:10.1111/j.1745-4603.1998.tb00170.x
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Jacobson, A. L., & Braun, H. (1987) · Biochemistry and Cell Biology · doi:10.1139/o87-055
Faidley, T. D., Luhman, C. M., Galloway, S. T., et al. (1999) · Nutrition Research · doi:10.1016/s0271-5317(99)00063-9
Sargi, S. C. (2013) · American Journal of Applied Sciences · doi:10.3844/ajassp.2013.367.373
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Umezawa, M., Okuyama, H., Hosokawa, M., et al. (2003) · International Congress Series · doi:10.1016/s0531-5131(03)01428-6
Ortiz-Vilchis, C. M. (2015) · Medicina e Investigación · doi:10.1016/j.mei.2015.02.009
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Sv, G., Raize, T. E., & Lm, S. (1978) · Bulletin of Experimental Biology and Medicine · doi:10.1007/bf00800278
Kanazawa, A., Watanabe, Y., & Fujimoto, K. (1996) · Lipids · doi:10.1007/bf02522932
Iversen, H., Fuglebakk, E., Holmsen, H., et al. (2002) · Platelets · doi:10.1080/0953770021000007795
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Friend, D. W., & Kramer, J. K. G. (1978) · Canadian Journal of Animal Science · doi:10.4141/cjas78-015
The isolation and characterisation of the plasma membrane fromHalobacterium salinarium
Brown, R. H., Bellingham, F., & Stevenson, J. (1972) · Antonie van Leeuwenhoek · doi:10.1007/bf02328118
Sakai, T., Okano, Y., Nozawa, Y., et al. (1991) · Journal of Bone and Mineral Metabolism · doi:10.1007/bf02374930
Lee, J., Kim, S. J., Chung, I.-S., et al. (2010) · Journal of The Korean Society of Water and Wastewater
Kim, S.-M., Kiyonaga, H., Yamaguchi, H., et al. (1999) · Bulletin of the Chemical Society of Japan · doi:10.1246/bcsj.72.541
Hiyama, K., Wada, S., & Kobayashi, O. (1996) · Biocontrol Science · doi:10.4265/bio.1.33
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Fonvieille, J.-L., Ecalle, R., & Dargent, R. (1992) · Mycopathologia · doi:10.1007/bf00472567
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Luxo, P. C., Jurado, A. S., Almeida, L. M., et al. (1994) · Toxicology in Vitro · doi:10.1016/0887-2333(94)90023-x
Kaya, K., Sano, T., & Shiraishi, F. (1992) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(92)90196-3
Morii, M., Murata, T., Suzuki, H., et al. (2000) · MEMBRANE · doi:10.5360/membrane.25.198
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Ryals, P. E., & Williams, N. E. (1999) · European Journal of Protistology · doi:10.1016/s0932-4739(99)80045-5
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Ignatova, E. V., Rubchevskaya, L. P., & Repyakh, S. M. (1994) · Chemistry of Natural Compounds · doi:10.1007/bf00629998
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Novgorodtseva, T. P., Karaman, Y. K., Gvozdenko, T. A., et al. (2013) · Health · doi:10.4236/health.2013.54a010
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Ушкалова, В. Н., Artamonova, N. A., Сторожок, Н. М., et al. (1981) · Chemistry of Natural Compounds · doi:10.1007/bf00565147
Zagayko, A. L., Kravchenko, G. B., & Krasilnikova, O. A. (2012) · Adipobiology · doi:10.14748/adipo.v4.284
Favre, B., Mauco, G., Turian, G., et al. (1991) · Plant Science · doi:10.1016/0168-9452(91)90182-8
Whitington, P. F., Black, D. D., Struve, W. G., et al. (1985) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(85)90271-8
Pelassy, C., Breittmayer, J., & Aussel, C. (1999) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1999.1841
Katyal, R., Rana, S. V., Ojha, S. K., et al. (2005) · Indian Journal of Clinical Biochemistry · doi:10.1007/bf02893055
Nutrient content of processed fish products common to the jewish diet
Pivarnik, L. F., Ferguson, E., Rheault, A., et al. (1989) · Journal of Food Composition and Analysis · doi:10.1016/0889-1575(89)90064-1
Benfenati, E., Perico, N., Peterlongo, F., et al. (1992) · Biochemical Medicine and Metabolic Biology · doi:10.1016/0885-4505(92)90068-a
Lachical, E., Maldonado, A. L., & Villanueva, E. (1989) · International Journal of Legal Medicine · doi:10.1007/bf00187564
Hepatic membranolytic stability alteration by metalloporphins in rats
Beri, R., & Chandra, R. (1991) · Journal of Inorganic Biochemistry · doi:10.1016/0162-0134(91)80047-l
Dupuis, F., Dulery, C., Praloran, V., et al. (1996) · Cancer Letters · doi:10.1016/0304-3835(96)04360-1
Fischer, J. W., Reading, S. A., Hillyer, L. M., et al. (2001) · Canadian Journal of Physiology and Pharmacology · doi:10.1139/cjpp-79-6-512
Havel, C. M., & Watson, J. A. (1992) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(92)90114-c
Shelf-life of a drum-dried high lysine sweet corn pulp
Rosenthal, A., Sgarbieri, V. C., & Deliza, R. (1991) · Food Control · doi:10.1016/0956-7135(91)90087-d
The Effect of Triton WR‐1339 on Lipid Metabolism in Mice
Aboul‐Enein, A. M., Rahim, E. A. A. E., Youssef, A. M., et al. (1986) · Fette Seifen Anstrichmittel · doi:10.1002/lipi.19860880607
Inhibition of Cytosolic Phospholipase A2 in Human Neutrophils by Oxatomide
Triggiani, M., Oriente, A., Granata, F., et al. (2001) · International Archives of Allergy and Immunology · doi:10.1159/000053760
Beersel, G. V. (2013) · The Open Pathology Journal · doi:10.2174/1874375720130905003
Hundertmark, S., Ragosch, V., Bühler, H., et al. (1996) · PerinatalMedizin · doi:10.1007/s001520050026
Кириченко, К. А., & Побежимова, Т. П. (2014) · Biologija · doi:10.6001/biologija.v59i3.2787
An HL-60 Variant Cell Line Defective in Cholesterol Synthesis.
Tsuyuki, M., & Yoshihara, K. (1995) · Cell Structure and Function · doi:10.1247/csf.20.13
Environmental influences on fatty acid composition of membranes from autoimmune MRL lpr/lpr mice
Hackshaw, K. V., Kunesh, S. L., Jackson, N. A., et al. (1996) · Prostaglandins Leukotrienes and Essential Fatty Acids · doi:10.1016/s0952-3278(96)90063-0
Hill, D. J., & Borysenko, J. Z. (1979) · The Journal of Cell Biology · doi:10.1083/jcb.83.1.218
Tsuruhara, K., Moreano, A. E., Juhn, S. K., et al. (1993) · Acta Oto-Laryngologica · doi:10.3109/00016489309126187
Metadata
- Authors
- Bligh, E. G., & Dyer, W. J.
- Venue
- Canadian Journal of Biochemistry and Physiology
- Publisher
- Canadian Science Publishing
- Published
- 1959-08-01
- Volume
- 37
- Issue
- 8
- Pages
- 911-917
- ISSN
- 0576-5544 (ISSN-L)
- OpenAlex
- W2152311269
- Cited by
- 54,751
- References
- 5
- Citations by year
- 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
- FWCI
- 8.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Canadian Journal of Biochemistry and Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
- Keywords
- lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
- MeSH terms
- Fishes, Lipids
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