Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 5,101–5,200 of 48,847

Tree species-mediated spatial patchiness of the composition of microbial community and physicochemical properties in the topsoils of a tropical montane forest
Ushio, M., Kitayama, K., & Balser, T. C. (2010) · Soil Biology and Biochemistry · doi:10.1016/j.soilbio.2010.05.035 · Cited by 103
Temporal changes in plasma thyroid hormone, growth hormone and free fatty acid concentrations, and hepatic 5?-monodeiodinase activity, lipid and protein content during chronic fasting and re-feeding in rainbow trout (Oncorhynchus mykiss)
Farbridge, K. J., & Leatherland, J. F. (1992) · Fish Physiology and Biochemistry · doi:10.1007/bf00004518 · Cited by 104
Cytochrome P450-derived renal HETEs: Storage and release
Carroll, M. A., Balazy, M., Huang, D.-D., et al. (1997) · Kidney International · doi:10.1038/ki.1997.234 · Cited by 104
A novel sphingolipid-TORC1 pathway critically promotes postembryonic development in Caenorhabditis elegans
Zhu, H., Shen, H., Sewell, A. K., Kniazeva, M., & Han, M. (2013) · eLife · doi:10.7554/elife.00429 · Cited by 103
Cis-9, trans-11-Conjugated Linoleic Acid Inhibits Allergic Sensitization and Airway Inflammation via a PPAR γ-Related Mechanism in Mice3
Jaudszus, A., Krokowski, M., Möckel, P., Darcan, Y., Avagyan, A., Matricardi, P. M., Jahreis, G., & Hamelmann, E. H. (2008) · Journal of Nutrition · doi:10.1093/jn/138.7.1336 · Cited by 104
Characterization of outer membranes isolated from Borrelia burgdorferi, the Lyme disease spirochete
Radolf, J. D., Goldberg, M. S., Bourell, K. W., Baker, S. I., Jones, J. D., & Norgard, M. V. (1995) · Infection and Immunity · doi:10.1128/iai.63.6.2154-2163.1995 · Cited by 103
Oxidative and lipolytic changes during ripening of Iberian hams as affected by feeding regime: extensive feeding and alpha-tocopheryl acetate supplementation
Cava, R., Ruiz, J., Ventanas, J., et al. (1999) · Meat Science · doi:10.1016/s0309-1740(98)00164-8 · Cited by 104
2-Hydrazinoquinoline as a Derivatization Agent for LC-MS-Based Metabolomic Investigation of Diabetic Ketoacidosis
Lu, Y., Yao, D., & Chen, C. (2013) · Metabolites · doi:10.3390/metabo3040993 · Cited by 101
Characteristics of Egg Yolk Phosvitin as an Antioxidant for Inhibiting Metal-Catalyzed Phospholipid Oxidations
LU, C.-L., & BAKER, R. C. (1986) · Poultry Science · doi:10.3382/ps.0652065 · Cited by 104
Multiple origins of hydrogenosomes: functional and phylogenetic evidence from the ADP/ATP carrier of the anaerobic chytridNeocallimastixsp.
Voncken, F. G. J., Boxma, B., Tjaden, J., Akhmanova, A. S., Huynen, M. A., Verbeek, F. J., Tielens, A. G. M., Haferkamp, I., Neuhaus, H. E., Vogels, G. D., et al. (2002) · Molecular Microbiology · doi:10.1046/j.1365-2958.2002.02959.x · Cited by 104
Phosphatidylglycerol Is Essential for Oligomerization of Photosystem I Reaction Center
Domonkos, I., Malec, P., Sallai, A., et al. (2004) · PLANT PHYSIOLOGY · doi:10.1104/pp.103.037754 · Cited by 104
Effect of lipid extraction on the interpretation of fish community trophic relationships determined by stable carbon and nitrogen isotopes
Murry, B. A., Farrell, J. M., Teece, M. A., et al. (2006) · Canadian Journal of Fisheries and Aquatic Sciences · doi:10.1139/f06-116 · Cited by 104
Multiple, Independently Regulated Pathways of Cholesterol Transport across the Intestinal Epithelial Cells
Iqbal, J., Anwar, K., & Hussain, M. M. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m301177200 · Cited by 104
Healthier lipid combination as functional ingredient influencing sensory and technological properties of low‐fat frankfurters
Delgado‐Pando, G., Cofrades, S., Ruíz‐Capillas, C., & Jiménez-Colmenero, F. (2010) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.201000076 · Cited by 104
Effects of pH and Temperature on the Composition of Polar Lipids in Thermoplasma acidophilum HO-62
Shimada, H., Nemoto, N., Shida, Y., et al. (2008) · Journal of Bacteriology · doi:10.1128/jb.00415-08 · Cited by 104
Target of rapamycin (TOR) plays a critical role in triacylglycerol accumulation in microalgae
Imamura, S., Kawase, Y., Kobayashi, I., Sone, T., Era, A., Miyagishima, S., Shimojima, M., Ohta, H., & Tanaka, K. (2015) · Plant Molecular Biology · doi:10.1007/s11103-015-0370-6 · Cited by 102
Genotypic and Phenotypic Responses of a Riverine Microbial Community to Polycyclic Aromatic Hydrocarbon Contamination
Langworthy, D. E., Stapleton, R. D., Sayler, G. S., et al. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.9.3422-3428.1998 · Cited by 104
The cellular mechanism of glucocorticoid acceleration of fetal lung maturation. Fibroblast-pneumonocyte factor stimulates choline-phosphate cytidylyltransferase activity.
Post, M., Barsoumian, A., & Smith, B. T. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)35914-8 · Cited by 104
Insulin stimulates receptor-mediated uptake of apoE-enriched lipoproteins and activated alpha 2-macroglobulin in adipocytes.
Descamps, O. S., Bilheimer, D., & Herz, J. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54029-1 · Cited by 104
Unsaturated Fatty Acid-mediated Decreases in Sterol Regulatory Element-mediated Gene Transcription Are Linked to Cellular Sphingolipid Metabolism
Worgall, T. S., Johnson, R. A., Seo, T., Gierens, H., & Deckelbaum, R. J. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m102393200 · Cited by 103
The perturbed membrane of cells undergoing apoptosis is susceptible to type II secretory phospholipase A2 to liberate arachidonic acid
Atsumi, G.-I., Murakami, M., Tajima, M., Shimbara, S., Hara, N., & Kudo, I. (1997) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/s0005-2760(97)00082-9 · Cited by 104
Fatty acid and molecular species compositions of phospholipids and diacylglycerols from rat retinal membranes
Stinson, A. M., Wiegand, R. D., & Anderson, R. E. (1991) · Experimental Eye Research · doi:10.1016/0014-4835(91)90261-c · Cited by 104
Seasonal partitioning and utilization of energy reserves in two age classes of the bay scallop Argopecten irradians irradians (Lamarck)
Epp, J., Bricelj, V. M., & Malouf, R. E. (1988) · Journal of Experimental Marine Biology and Ecology · doi:10.1016/0022-0981(88)90250-x · Cited by 104
Specificity and binding affinity of phospholipids to the high-affinity cardiolipin sites of beef heart cytochrome c oxidase
Robinson, N. C. (1982) · Biochemistry · doi:10.1021/bi00530a031 · Cited by 104
Regional Levels of Free Fatty Acids and Evans Blue Extravasation After Experimental Brain Injury
Dhillon, H. S., Donaldson, D. L., Dempsey, R. J., & Prasad, M. R. (1994) · Journal of Neurotrauma · doi:10.1089/neu.1994.11.405 · Cited by 104
Diacylglycerol accumulation and superoxide anion production in stimulated human neutrophils.
Rider, L. G., & Niedel, J. E. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)45616-5 · Cited by 104
Resonance energy transfer microscopy: observations of membrane-bound fluorescent probes in model membranes and in living cells.
Uster, P. S., & Pagano, R. E. (1986) · The Journal of Cell Biology · doi:10.1083/jcb.103.4.1221 · Cited by 104
Chemical, enzymological and permeability properties of human erythrocyte ghosts prepared by hypotonic lysis in media of different osmolarities
Bramley, T. A., COLEMAN, R., & FINEAN, J. B. (1971) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(71)90003-4 · Cited by 104
Precursor-product relationship between rabbit type II cell lamellar bodies and alveolar surface-active material
Baritussio, A., Magoon, M. W., Goerke, J., & Clements, J. A. (1981) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(81)90297-6 · Cited by 104
Evidence for existence of various homologues and analogues of platelet activating factor in a lipid extract of bovine brain
Tokumura, A., Kamiyasu, K., Takauchi, K., et al. (1987) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(87)91338-6 · Cited by 104
Studies on Seed‐Oil Triglycerides
GURR, M. I., Blades, J., Appleby, R. S., et al. (1974) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1974.tb03411.x · Cited by 104
Developmental regulation of a stalk cell differentiation-inducing factor in Dictyostelium discoideum
Brookman, J. J., Town, C. D., Jermyn, K. A., et al. (1982) · Developmental Biology · doi:10.1016/0012-1606(82)90022-7 · Cited by 104
Amyloid-β induces lipid droplet-mediated microglial dysfunction via the enzyme DGAT2 in Alzheimer’s disease
Prakash, P., Manchanda, P., Paouri, E., Bisht, K., Sharma, K., Rajpoot, J., Wendt, V., Hossain, A., Wijewardhane, P. R., Randolph, C. E., et al. (2025) · Immunity · doi:10.1016/j.immuni.2025.04.029 · Cited by 104
Metabolic profiles of Nannochloropsis oceanica IMET1 under nitrogen-deficiency stress
Xiao, Y., Zhang, J., Cui, J., Feng, Y., & Cui, Q. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2012.11.116 · Cited by 101
Repression of proinflammatory gene expression by lipid extract of Nostoc commune var sphaeroides Kützing, a blue-green alga, via inhibition of nuclear factor-κB in RAW 264.7 macrophages
Park, Y.-K., Rasmussen, H. E., Ehlers, S. J., Blobaum, K. R., Lu, F., Schlegal, V. L., Carr, T. P., & Lee, J.-Y. (2008) · Nutrition Research · doi:10.1016/j.nutres.2007.11.008 · Cited by 103
Low‐ammonia niche of ammonia‐oxidizing archaea in rotating biological contactors of a municipal wastewater treatment plant
Sauder, L. A., Peterse, F., Schouten, S., & Neufeld, J. D. (2012) · Environmental Microbiology · doi:10.1111/j.1462-2920.2012.02786.x · Cited by 104
Characterization of oleaginous yeasts accumulating high levels of lipid when cultivated in glycerol and their potential for lipid production from biodiesel-derived crude glycerol
Polburee, P., Yongmanitchai, W., Lertwattanasakul, N., et al. (2015) · Fungal Biology · doi:10.1016/j.funbio.2015.09.002 · Cited by 103
Variations in microbial carbon sources and cycling in the deep continental subsurface
Simkus, D. N., Slater, G. F., Lollar, B. S., Wilkie, K. M., Kieft, T. L., Magnabosco, C., Lau, M. C. Y., Pullin, M. J., Hendrickson, S. B., Wommack, K. E., et al. (2015) · Geochimica et Cosmochimica Acta · doi:10.1016/j.gca.2015.10.003 · Cited by 102
Krill oil versus fish oil in modulation of inflammation and lipid metabolism in mice transgenic for TNF-α
Vigerust, N. F., Bjørndal, B., Bohov, P., Brattelid, T., Svardal, A. M., & Berge, R. K. (2012) · European Journal of Nutrition · doi:10.1007/s00394-012-0441-2 · Cited by 105
Stearoyl-CoA Desaturase-1 (SCD1) Augments Saturated Fatty Acid-Induced Lipid Accumulation and Inhibits Apoptosis in Cardiac Myocytes
Matsui, H., Yokoyama, T., Sekiguchi, K., Iijima, D., Sunaga, H., Maniwa, M., Ueno, M., Iso, T., Arai, M., & Kurabayashi, M. (2012) · PLoS ONE · doi:10.1371/journal.pone.0033283 · Cited by 102
Generation of phospholipid artefacts during extraction of developing soybean seeds with methanolic solvents
Roughan, P. G., Slack, C. R., & Holland, R. (1978) · Lipids · doi:10.1007/bf02533620 · Cited by 103
The effect of iron on growth, lipid accumulation, and gene expression profile of the freshwater microalga Chlorella sorokiniana
Wan, M., Jin, X., Xia, J., Rosenberg, J. N., Yu, G., Nie, Z., Oyler, G. A., & Betenbaugh, M. J. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-6088-6 · Cited by 104
Polar lipids in bovine milk II. Long-chain bases, normal and 2-hydroxy fatty acids, and isomeric cis and trans monoenoic fatty acids in the sphingolipids
Morrison, W. R., & Hay, J. D. (1970) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(70)90116-5 · Cited by 103
Unraveling Barrier Properties of Three Different In-House Human Skin Equivalents
Thakoersing, V. S., Gooris, G. S., Mulder, A. A., Rietveld, M. H., Ghalbzouri, A. E., & Bouwstra, J. A. (2011) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2011.0175 · Cited by 102
Predictive value of bovine follicular components as markers of oocyte developmental potential
Matoba, S., Bender, K., Fahey, A. G., et al. (2013) · Reproduction Fertility and Development · doi:10.1071/rd13007 · Cited by 103
Simple and fast HPLC method for simultaneous determination of retinol, tocopherols, coenzyme Q10 and carotenoids in complex samples
Gleize, B., Steib, M., André, M., & Reboul, E. (2012) · Food Chemistry · doi:10.1016/j.foodchem.2012.04.043 · Cited by 104
Oxysterol-Induced Soluble Endoglin Release and Its Involvement in Hypertension
Valbuena-Diez, A. C., Blanco, F. J., Oujo, B., Langa, C., González-Núñez, M., Llano, E., Pendás, A. M., Díaz, M., Castrillo, A., López-Novoa, J. M., et al. (2012) · Circulation · doi:10.1161/circulationaha.112.101261 · Cited by 103
Long-term moderate intervention with n-3 long-chain PUFA-supplemented dairy products: effects on pathophysiological biomarkers in patients with rheumatoid arthritis
Dawczynski, C., Schubert, R., Hein, G. E., Müller, A., Eidner, T., Vogelsang, H., Basu, S., & Jahreis, G. (2009) · British Journal Of Nutrition · doi:10.1017/s0007114508076216 · Cited by 99
Enhancement of lipid extraction for improved biodiesel recovery from the biodiesel promising microalga Scenedesmus obliquus
Abomohra, A., Jin, W., & El-Sheekh, M. M. (2015) · Energy Conversion and Management · doi:10.1016/j.enconman.2015.11.007 · Cited by 101
Influence of cyclopropane fatty acids on heat, high pressure, acid and oxidative resistance in Escherichia coli
Chen, Y. Y., & Gänzle, M. G. (2016) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2016.01.017 · Cited by 103
Down-regulation of HSP60 Suppresses the Proliferation of Glioblastoma Cells via the ROS/AMPK/mTOR Pathway
Tang, H., Li, J., Liu, X., Wang, G., Luo, M., & Deng, H. (2016) · Scientific Reports · doi:10.1038/srep28388 · Cited by 106
Ceramide-induced BOK promotes mitochondrial fission in preeclampsia
Ausman, J., Abbade, J. F., Ermini, L., Farrell, A., Tagliaferro, A., Post, M., & Caniggia, I. (2018) · Cell Death and Disease · doi:10.1038/s41419-018-0360-0 · Cited by 107
Extraction and purification of eicosapentaenoic acid and docosahexaenoic acid from microalgae: A critical review
Li, X., Liu, J., Chen, G., Zhang, J., Wang, C., & Liu, B. (2019) · Algal Research · doi:10.1016/j.algal.2019.101619 · Cited by 111
Avocado–Soybean Unsaponifiables: A Panoply of Potentialities to Be Exploited
Salehi, B., Rescigno, A., Dettori, T., Călina, D. C., Docea, A. O., Singh, L., Cebeci, F., Özçelik, B., Bhia, M., Beirami, A. D., et al. (2020) · Biomolecules · doi:10.3390/biom10010130 · Cited by 103
Chia (Salvia hispanica L.) mucilage as a new fat substitute in emulsified meat products: Technological, physicochemical, and rheological characterization
Câmara, A. K. F. I., Okuro, P. K., Cunha, R. L., Herrero, A. M., Ruíz‐Capillas, C., & Pollonio, M. A. R. (2020) · LWT · doi:10.1016/j.lwt.2020.109193 · Cited by 108
Using inulin‐based emulsion gels as fat substitute in salt reduced Bologna sausage
Paglarini, C. D. S., Vidal, V. A. S., Ribeiro, W. O., et al. (2020) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.10659 · Cited by 103
Purple bacteria as added-value protein ingredient in shrimp feed: Penaeus vannamei growth performance, and tolerance against Vibrio and ammonia stress
Alloul, A., Wille, M., Lucenti, P., Bossier, P., Stappen, G. V., & Vlaeminck, S. E. (2020) · Aquaculture · doi:10.1016/j.aquaculture.2020.735788 · Cited by 104
Açaí seeds: An unexplored agro-industrial residue as a potential source of lipids, fibers, and antioxidant phenolic compounds
Melo, P. S., Selani, M. M., Gonçalves, R. H., Paulino, J. D. O., Massarioli, A. P., & de Alencar, S. M. (2020) · Industrial Crops and Products · doi:10.1016/j.indcrop.2020.113204 · Cited by 104
Human serum triggers antibiotic tolerance in Staphylococcus aureus
Ledger, E. V. K., Mesnage, S., & Edwards, A. M. (2022) · Nature Communications · doi:10.1038/s41467-022-29717-3 · Cited by 97
A phospholipid uptake system in the model plant Arabidopsis thaliana
Poulsen, L. R., López‐Marqués, R. L., Pedas, P., et al. (2015) · Nature Communications · doi:10.1038/ncomms8649 · Cited by 103
Metabolism of Phosphatidylglycerol, Phosphatidylethanolamine, and Cardiolipin of Bacillus stearothermophilus
Card, G. L. (1973) · Journal of Bacteriology · doi:10.1128/jb.114.3.1125-1137.1973 · Cited by 103
Enzymatic hydrolysis of cod (Gadus morhua) by-products
Šližytė, R., Rustad, T., & Storrø, I. (2005) · Process Biochemistry · doi:10.1016/j.procbio.2005.04.007 · Cited by 103
Purification and characterization of oil-bodies (oleosomes) and oil-body boundary proteins (oleosins) from the developing cotyledons of sunflower (Helianthus annuus L.)
Millichip, M. I., Tatham, A. S., Jackson, F. M., et al. (1996) · Biochemical Journal · doi:10.1042/bj3140333 · Cited by 103
The effect of microbial inoculation and pH on microbial community structure changes during composting
Lei, F., & VanderGheynst, J. S. (2000) · Process Biochemistry · doi:10.1016/s0032-9592(99)00155-7 · Cited by 103
Lipid Characterization of an Enriched Plasma Membrane Fraction of Dunaliella salina Grown in Media of Varying Salinity
Peeler, T. C., Stephenson, M. B., Einspahr, K. J., & Thompson, G. A. (1989) · PLANT PHYSIOLOGY · doi:10.1104/pp.89.3.970 · Cited by 102
Determination of the nutritional value, protein quality and safety of krill protein concentrate isolated using an isoelectric solubilization/precipitation technique
Gigliotti, J. C., Jaczynski, J., & Tou, J. C. L. (2008) · Food Chemistry · doi:10.1016/j.foodchem.2008.03.030 · Cited by 103
The potential of Raman spectroscopy for characterisation of the fatty acid unsaturation of salmon
Afseth, N. K., Wold, J. P., & Segtnan, V. H. (2006) · Analytica Chimica Acta · doi:10.1016/j.aca.2006.05.013 · Cited by 103
The effect of increasing carcass weight of finishing boars and gilts on joint composition and meat quality
Beattie, V. E., Weatherup, R. N., MOSS, B. W., et al. (1999) · Meat Science · doi:10.1016/s0309-1740(98)00169-7 · Cited by 103
Cultured copepods as food for West Australian dhufish (Glaucosoma hebraicum) and pink snapper (Pagrus auratus) larvae
Payne, M. F., Rippingale, R. J., & Cleary, J. J. (2001) · Aquaculture · doi:10.1016/s0044-8486(00)00513-5 · Cited by 103
Nutrient supplementation with polyunsaturated fatty acids and micronutrients in rheumatoid arthritis: clinical and biochemical effects
Remans, P. H. J., Sont, J. K., Wagenaar, L. W., Wouters-Wesseling, W., Zuijderduin, W. M., Jongma, A., Breedveld, F. C., & van Laar, J. M. (2004) · European Journal of Clinical Nutrition · doi:10.1038/sj.ejcn.1601883 · Cited by 101
Sphingomyelin synthases regulate production of diacylglycerol at the Golgi
Villani, M., Subathra, M., Im, Y., Choi, Y. L., Signorelli, P., Poeta, M. D., & Luberto, C. (2008) · Biochemical Journal · doi:10.1042/bj20071240 · Cited by 103
Proteolytic Extraction of Salmon Oil and PUFA Concentration by Lipases
Linder, M., Fanni, J., & Parmentier, M. (2005) · Marine Biotechnology · doi:10.1007/s10126-004-0149-2 · Cited by 108
Asian seabass Lates calcarifer perform well when fed pelleted diets high in protein and lipid
Williams, K. C., Barlow, C. G., Rodgers, L., et al. (2003) · Aquaculture · doi:10.1016/s0044-8486(03)00278-3 · Cited by 103
Elimination of the CDP-ethanolamine Pathway Disrupts Hepatic Lipid Homeostasis
Leonardi, R., Frank, M. W., Jackson, P. D., et al. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.031336 · Cited by 103
Micro‐scale distribution of microorganisms and microbial enzyme activities in a soil with long‐term organic amendment
Poll, C., Thiede, A., Wermbter, N., Sessitsch, A., & Kandeler, E. (2003) · European Journal of Soil Science · doi:10.1046/j.1351-0754.2003.0569.x · Cited by 102
Isolation of a Chinese Hamster Ovary (CHO) cDNA Encoding Phosphatidylglycerophosphate (PGP) Synthase, Expression of Which Corrects the Mitochondrial Abnormalities of a PGP Synthase-defective Mutant of CHO-K1 Cells
Kawasaki, K., Kuge, O., Chang, S.-C., Heacock, P. N., Rho, M., Suzuki, K., Nishijima, M., & Dowhan, W. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.3.1828 · Cited by 101
Impaired Coordination of Nutrient Intake and Substrate Oxidation in Melanocortin-4 Receptor Knockout Mice
Albarado, D. C., McClaine, J. W., Stephens, J. M., Mynatt, R. L., Ye, J., Bannon, A. W., Richards, W. G., & Butler, A. A. (2003) · Endocrinology · doi:10.1210/en.2003-0452 · Cited by 103
Characterization of Phospholipids in Membrane Vesicles Derived fromPseudomonas aeruginosa
Tashiro, Y., Inagaki, A., Shimizu, M., et al. (2011) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.100754 · Cited by 103
The degradation of bisphenol A by the newly isolated bacterium Cupriavidus basilensis JF1 can be enhanced by biostimulation with phenol
Fischer, J., Kappelmeyer, U., Kästner, M., et al. (2010) · International Biodeterioration & Biodegradation · doi:10.1016/j.ibiod.2010.03.007 · Cited by 103
Phospholipid biosynthesis and secretion by a cell line (A549) which resembles type II alveolar epithelial cells
Shapiro, D. L., Nardone, L. L., Rooney, S. A., et al. (1978) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(78)90005-x · Cited by 103
Influence of weaning on carcass quality, fatty acid composition and meat quality in intensive lamb production systems
Sañudo, C., Sierra, I., Olleta, J. L., et al. (1998) · Animal Science · doi:10.1017/s1357729800008948 · Cited by 103
Phospholipid composition of in vitro endothelial microparticles and their in vivo thrombogenic properties
Hussein, M. N. A., Böing, A. N., Biró, É., Hoek, F. J., Vogel, G. M. T., Meuleman, D. G., Sturk, A., & Nieuwland, R. (2007) · Thrombosis Research · doi:10.1016/j.thromres.2007.08.005 · Cited by 103
PHOSPHOLIPID COMPOSITION OF HUMAN SPERM AND SEMINAL PLASMA IN RELATION TO SPERM FERTILITY
Gulaya, V. M. M. N. M. (2001) · Archives of Andrology · doi:10.1080/01485010151096405 · Cited by 103
N-(4-Hydroxyphenyl)retinamide increases dihydroceramide and synergizes with dimethylsphingosine to enhance cancer cell killing
Wang, H., Maurer, B. J., Liu, Y., et al. (2008) · Molecular Cancer Therapeutics · doi:10.1158/1535-7163.mct-08-0549 · Cited by 103
Intrahepatic Mechanisms Underlying the Effect of Metformin in Decreasing Basal Glucose Production in Rats Fed a High-Fat Diet
Mithieux, G., Guignot, L., Bordet, J., & Wiernsperger, N. F. (2002) · Diabetes · doi:10.2337/diabetes.51.1.139 · Cited by 108
Involvement of Cyclopropane Fatty Acids in the Response of Pseudomonas putida KT2440 to Freeze-Drying
Muñoz‐Rojas, J., Bernal, P., Duque, E., Godoy, P., Segura, A., & Ramos, J. L. (2006) · Applied and Environmental Microbiology · doi:10.1128/aem.72.1.472-477.2006 · Cited by 103
Lipid compositional analysis of pulmonary surfactant monolayers and monolayer-associated reservoirs
Yu, S.-H., & Possmayer, F. (2003) · Journal of Lipid Research · doi:10.1194/jlr.m200380-jlr200 · Cited by 103
Comparative studies on the nutrition of two species of abalone, Haliotis tuberculata L. and Haliotis discus hannai Ino.
Mai, K. (1998) · Aquaculture · doi:10.1016/s0044-8486(97)00286-x · Cited by 103
Effect of dietary oils and alpha-tocopheryl acetate supplementation on lipid (TBARS) and cholesterol oxidation in cooked pork.
Rey, A. I., Kerry, J. P., Lynch, P. B., et al. (2001) · Journal of Animal Science · doi:10.2527/2001.7951201x · Cited by 103
Technological properties and bacteriocins production by Lactobacillus curvatus 54M16 and its use as starter culture for fermented sausage manufacture
Casaburi, A., Martino, V. D., Ferranti, P., Picariello, G., & Villani, F. (2015) · Food Control · doi:10.1016/j.foodcont.2015.05.016 · Cited by 108
Oxygen requirements for formation and activity of the squalene epoxidase in Saccharomyces cerevisiae
Jahnke, L. L., & Klein, H. P. (1983) · Journal of Bacteriology · doi:10.1128/jb.155.2.488-492.1983 · Cited by 103
Relationship of hyperthermia-induced hemolysis of human erythrocytes to the thermal denaturation of membrane proteins
Lepock, J. R., Frey, H. E., Bayne, H. G., et al. (1989) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(89)90399-4 · Cited by 103
Nonoxidative ethanol metabolism: formation of fatty acid ethyl esters by cholesterol esterase.
Lange, L. G. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.13.3954 · Cited by 103
Alteration of the Sphingomyelin/Ceramide Pathway Is Associated with Resistance of Human Breast Carcinoma MCF7 Cells to Tumor Necrosis Factor-α-mediated Cytotoxicity
Cai, Z., Bettaı̈eb, A., Mahdani, N. E., Legrès, L., Stancou, R., Masliah, J., & Chouaı̈b, S. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.11.6918 · Cited by 103
Bromoenol Lactone Promotes Cell Death by a Mechanism Involving Phosphatidate Phosphohydrolase-1 Rather than Calcium-independent Phospholipase A2
Fuentes, L., Pérez, R., Nieto, M. L., Balsinde, J., & Balboa, M. A. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m307209200 · Cited by 103
A Comparison between Prolamellar Bodies and Prothylakoid Membranes of Etioplasts of Dark-Grown Wheat Concerning Lipid and Polypeptide Composition
Selstam, E., & Sandelius, A. S. (1984) · PLANT PHYSIOLOGY · doi:10.1104/pp.76.4.1036 · Cited by 103
Lipoproteins alter the catalytic behavior of the platelet-activating factor acetylhydrolase in human plasma.
Stafforini, D. M., Carter, M. E., Zimmerman, G. A., et al. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.7.2393 · Cited by 103
The transmembrane domain of glycophorin A as studied by cross-linking using photoactivatable phospholipids.
Ross, A. H., Radhakrishnan, R., Robson, R. J., et al. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)34699-4 · Cited by 103
In vivo and in vitro chemotactic methylation in Bacillus subtilis
Ullah, A., & Ordal, G. (1981) · Journal of Bacteriology · doi:10.1128/jb.145.2.958-965.1981 · Cited by 103
Manipulation of the Phospholipid Composition of Tissue Culture Cells
Gläser, M., Ferguson, K. A., & Vagelos, P. R. (1974) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.71.10.4072 · Cited by 103

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks