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Cited by 103Seaweed-derived Bioactive CompoundsNatural Compounds in Disease TreatmentNF-κB Signaling Pathways

Repression of proinflammatory gene expression by lipid extract of Nostoc commune var sphaeroides Kützing, a blue-green alga, via inhibition of nuclear factor-κB in RAW 264.7 macrophages

Park, Young-Ki, Rasmussen, Heather E., Ehlers, Sarah J., Blobaum, Kara R., Lu, Fan, Schlegal, Vicki L., Carr, Timothy P., Lee, Ji-Young·2008·Nutrition Research
DOI 10.1016/j.nutres.2007.11.008
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Abstract

We investigated whether lipid extract from a blue-green alga, N commune, modulates proinflammatory gene expression in RAW 264.7 macrophages. The cells were incubated with N commune lipid extract (0-100 microg/mL) and subsequently activated by LPS (100 ng/mL). Quantitative real-time PCR analysis showed that mRNA abundance of proinflammatory mediators, including TNF-alpha, COX-2, IL-1beta, IL-6, and iNOS, was significantly reduced by N commune lipid extract in a dose-dependent manner. Secretion of TNF-alpha and IL-1beta into cell culture medium was also significantly decreased by N commune lipid extract. Thin-layer chromatography-densitometry analysis showed that N commune lipid extract contained approximately 15% of fatty acids. To determine whether the inhibition of proinflammatory mediator production by N commune lipid extract is primarily conferred by fatty acids in the lipid extract, macrophages were incubated with 100 microg/mL of N commune lipid extract or 15 microg/mL of a fatty acid mixture, which was formulated to reflect the fatty acid composition of N commune lipid extract. The fatty acid mixture significantly reduced RNA abundance of TNF-alpha and COX-2, but to a lesser extent than did the N commune lipid extract, suggesting the presence of additional bioactive compounds with an antiinflammatory property in the lipid extract. As NF-kappaB is a major regulator for the proinflammatory gene expression, we measured its DNA-binding activity. DNA-binding activity of NF-kappaB was significantly reduced by N commune lipid extract. In conclusion, our study suggests that N commune lipid extract represses the expression of proinflammatory genes in RAW 264.7 macrophages, at least in part, by inhibiting the activation of NF-kappaB pathway.

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Authors
Park, Y.-K., Rasmussen, H. E., Ehlers, S. J., Blobaum, K. R., Lu, F., Schlegal, V. L., Carr, T. P., & Lee, J.-Y.
Affiliations
  • University of Nebraska–Lincoln (US)
  • Algaen (United States) (US)
ORCID
Venue
Nutrition Research
Publisher
Elsevier BV
Published
2008-02
Volume
28
Issue
2
Pages
83-91
ISSN
0271-5317 (ISSN-L)
DOI
10.1016/j.nutres.2007.11.008
OpenAlex
W1991552651
Cited by
103
References
46
Citations by year
2026: 3 · 2024: 5 · 2023: 1 · 2022: 8 · 2021: 10 · 2020: 9 · 2019: 6 · 2018: 5 · 2017: 4 · 2016: 5 · 2015: 7 · 2014: 9 · 2013: 5 · 2012: 4
FWCI
11.01
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Nutrition Research (publishedVersion)
  • PubMed (publishedVersion)
  • Lincoln (University of Nebraska) (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
  • Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
Keywords
Nostoc commune, cyanobacteria, anti-inflammatory activity, pro-inflammatory gene expression, NF-κB inhibition, RAW 264.7 macrophages, lipopolysaccharide-induced inflammation, TNF-α, cyclooxygenase-2, IL-1β, interleukin-6, inducible nitric oxide synthase, fatty acids, DNA-binding activity
MeSH terms
Cells, Cultured, Chromatography, Thin Layer, Densitometry, Dose-Response Relationship, Drug, Gene Expression Regulation, Humans, Lipids, Lipopolysaccharides, Macrophages, RNA, Messenger, Polymerase Chain Reaction, NF-kappa B, Nostoc commune

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