Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 9,001–9,100 of 48,846

Effects of dietary hemp seed oil to sows on fatty acid profiles, nutritional and immune status of piglets
Vodolazs'ka, D., & Lauridsen, C. (2020) · Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · doi:10.1186/s40104-020-0429-3 · Cited by 68
“Prior to fatty acid analysis of milk, milk samples were thawed and gently mixed in a water bath held at 40 °C. Fat was extracted from the milk according to the method by Bligh and Dyer [28].”
MethodInfluential
SPHK2-Generated S1P in CD11b+ Macrophages Blocks STING to Suppress the Inflammatory Function of Alveolar Macrophages
Joshi, J. C., Joshi, B., Rochford, I., Rayees, S., Akhter, M. Z., Baweja, S., Chava, K. R., Tauseef, M., Abdelkarim, H., Natarajan, V., et al. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.02.112 · Cited by 68
FIT2 is an acyl–coenzyme A diphosphatase crucial for endoplasmic reticulum homeostasis
Becuwe, M., Bond, L. M., Pinto, A. F. M., Boland, S., Mejhert, N., Elliott, S. D., Cicconet, M., Graham, M. M., Liu, X. N., Ilkayeva, O., et al. (2020) · The Journal of Cell Biology · doi:10.1083/jcb.202006111 · Cited by 66
Functional Characteristics of Lactobacillus and Yeast Single Starter Cultures in the Ripening Process of Dry Fermented Sausage
Liu, Y., Wan, Z., Woldemariam, K. Y., et al. (2021) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.611260 · Cited by 68
The structure, viscoelasticity and charge of potato peptides adsorbed at the oil-water interface determine the physicochemical stability of fish oil-in-water emulsions
García‐Moreno, P. J., Yang, J., Echers, S. G., et al. (2021) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2021.106605 · Cited by 68
Particulate Metabolites and Transcripts Reflect Diel Oscillations of Microbial Activity in the Surface Ocean
Boysen, A. K., Carlson, L. T., Durham, B. P., Groussman, R. D., Aylward, F. O., Ribalet, F., Heal, K. R., White, A., DeLong, E. F., Armbrust, E. V., et al. (2021) · mSystems · doi:10.1128/msystems.00896-20 · Cited by 64
“Metabolite extractions employed a modified Bligh-Dyer method (4, 13, 88), resulting in aqueous and organic soluble metabolites with heavy stable isotope-labeled extraction and injection internal standards added to both fractions (Text S1, “metabolite sample extraction”).”
Method
Branched-chain amino acid metabolism controls membrane phospholipid structure in Staphylococcus aureus
Frank, M. W., Whaley, S., & Rock, C. O. (2021) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2021.101255 · Cited by 68
“Lipids were extracted from 5 ml of culture (52), and the dried lipids were suspended in chloroform/methanol (1:1).”
Method
Current Progress in Lipidomics of Marine Invertebrates
Imbs, A. B., Ermolenko, E. V., Grigorchuk, V. P., Sikorskaya, T. V., & Velansky, P. V. (2021) · Marine Drugs · doi:10.3390/md19120660 · Cited by 70
“Wang et al. [73] purchased dried sea cucumbers from a free market and carried out extraction according to Bligh and Dyer [88].”
Method
Enhancing the nutritional value of cold-pressed oilseed cakes through extrusion cooking
Vidal, N. P., Román, L., Swaraj, V. J. S., et al. (2022) · Innovative Food Science & Emerging Technologies · doi:10.1016/j.ifset.2022.102956 · Cited by 68
Influence of supplementation of all-rac-α-tocopheryl acetate preweaning and vitamin C postweaning on α-tocopherol and immune responses of piglets1
Lauridsen, C., & Jensen, S. K. (2005) · Journal of Animal Science · doi:10.2527/2005.8361274x · Cited by 68
Construction and characteristics of artificial consortia of Scenedesmus obliquus-bacteria for S. obliquus growth and lipid production
Wang, R., Xue, S., Zhang, D., Zhang, Q., Wen, S., Kong, D., Yan, C., & Cong, W. (2015) · Algal Research · doi:10.1016/j.algal.2015.10.002 · Cited by 67
Organ Damage and Hepatic Lipid Accumulation in Carp (Cyprinus carpio L.) after Feed-Borne Exposure to the Mycotoxin, Deoxynivalenol (DON)
Pietsch, C., Schulz, C., Rovira, P., et al. (2014) · Toxins · doi:10.3390/toxins6020756 · Cited by 68
Effect of fertilizer, herbicide and grazing management of pastures on plant and soil communities
Parfitt, R. L., Yeates, G. W., Ross, D. J., et al. (2010) · Applied Soil Ecology · doi:10.1016/j.apsoil.2010.03.010 · Cited by 68
Changing land use reduces soil CH4 uptake by altering biomass and activity but not composition of high‐affinity methanotrophs
Menyailo, O. V., Hungate, B. A., Abraham, W., & Conrad, R. (2008) · Global Change Biology · doi:10.1111/j.1365-2486.2008.01648.x · Cited by 69
SC-435, an ileal apical sodium co-dependent bile acid transporter (ASBT) inhibitor lowers plasma cholesterol and reduces atherosclerosis in guinea pigs
West, K. L., Zern, T. L., Butteiger, D. N., Keller, B. T., & Fernández, M. L. (2003) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2003.08.019 · Cited by 68
Capacitation status of fresh and frozen-thawed buffalo spermatozoa in relation to cholesterol level, membrane fluidity and intracellular calcium
Kadirvel, G., Kumar, S., Kumaresan, A., et al. (2009) · Animal Reproduction Science · doi:10.1016/j.anireprosci.2009.02.003 · Cited by 68
An Inner Membrane Dioxygenase that Generates the 2-Hydroxymyristate Moiety of Salmonella Lipid A
Gibbons, H. S., Reynolds, C. M., Guan, Z., & Raetz, C. R. H. (2008) · Biochemistry · doi:10.1021/bi702457c · Cited by 68
Physical and chemical characteristics of donkey meat from Martina Franca breed
Polidori, P., Cavallucci, C., Beghelli, D., et al. (2009) · Meat Science · doi:10.1016/j.meatsci.2009.03.001 · Cited by 68
Changes in amino acids and lipids during embryogenesis of European lobster, Homarus gammarus (Crustacea: Decapoda)
Rosa, R., Calado, R., Andrade, A. M., et al. (2004) · Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology · doi:10.1016/j.cbpc.2004.10.009 · Cited by 68
Short-term bioavailability of carbon in soil organic matter fractions of different particle sizes and densities in grassland ecosystems
Breulmann, M., Masyutenko, N. P., Kogut, B. M., et al. (2014) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2014.07.080 · Cited by 68
Physiological basis for energy demands and early postlarval mortality in the Pacific oyster, Crassostrea gigas
García-Esquivel, Z., Bricelj, V. M., & González-Gómez, M. A. (2001) · Journal of Experimental Marine Biology and Ecology · doi:10.1016/s0022-0981(01)00300-8 · Cited by 68
Effects of photoperiod, salinity and pH on cell growth and lipid content of Pavlova lutheri
Shah, S. M. U., Radziah, C. M. Z. C., Ibrahim, S. B., Latiff, F. A., Othman, M. F., & Abdullah, M. A. (2013) · Annals of Microbiology · doi:10.1007/s13213-013-0645-6 · Cited by 69
Seafood diets: Hypolipidemic and antiatherogenic effects of taurine and n-3 fatty acids
Elvevoll, E. O., Eilertsen, K., Brox, J. H., et al. (2008) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2007.12.021 · Cited by 68
Metabolic diversity in biohydrogenation of polyunsaturated fatty acids by lactic acid bacteria involving conjugated fatty acid production
Kishino, S., Ogawa, J., Yokozeki, K., & Shimizu, S. (2009) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-009-1949-0 · Cited by 68
Fatty acid composition of Allium species lipids
Tsiaganis, M. C., Laskari, K., & Melissari, E. (2006) · Journal of Food Composition and Analysis · doi:10.1016/j.jfca.2005.06.003 · Cited by 68
Isolation and characterization of a phospholipase B from venom of collett's snake, Pseudechis colletti
Bernheimer, A. W., Linder, R., Weinstein, S. A., et al. (1987) · Toxicon · doi:10.1016/0041-0101(87)90290-x · Cited by 68
Myosin-cross-reactive antigens from four different lactic acid bacteria are fatty acid hydratases
Yang, B. K., Chen, H., Song, Y., Chen, Y. Q., Zhang, H., & Chen, W. (2012) · Biotechnology Letters · doi:10.1007/s10529-012-1044-y · Cited by 69
Smoking reduces surfactant protein D and phospholipids in patients with and without chronic obstructive pulmonary disease
Moré, J. M., Voelker, D. R., Silveira, L. J., Edwards, M. G., Chan, E. D., & Bowler, R. P. (2010) · BMC Pulmonary Medicine · doi:10.1186/1471-2466-10-53 · Cited by 68
Lipid and fatty acid composition of milkfish (Chanos chanos Forsskal) grown in freshwater and seawater
Borlongan, I. G., & Benitez, L. V. (1992) · Aquaculture · doi:10.1016/0044-8486(92)90139-c · Cited by 68
Dietary Carnitine and Lysine Affect Channel Catfish Lipid and Protein Composition
Burtle, G. J., & Liu, Q. (1994) · Journal of the World Aquaculture Society · doi:10.1111/j.1749-7345.1994.tb00178.x · Cited by 68
Meiofauna distribution and mesoscale variability in two sites of the Ross Sea (Antarctica) with contrasting food supply
Fabiano, M., & Danovaro, R. (1999) · Polar Biology · doi:10.1007/s003000050398 · Cited by 68
Effect of Chenodeoxycholic Acid on Fibrosis, Inflammation and Oxidative Stress in Kidney in High-Fructose-Fed Wistar Rats
Hu, Z., Ren, L., Wang, C., Liu, B., & Song, G. (2012) · Kidney & Blood Pressure Research · doi:10.1159/000341485 · Cited by 68
Biochemical composition of Aurelia aurita in relation to age and sexual maturity
Lucas, C. H. (1994) · Journal of Experimental Marine Biology and Ecology · doi:10.1016/0022-0981(94)90086-8 · Cited by 68
Prolonged nerve blockade delays the onset of neuropathic pain
Shankarappa, S. A., Tsui, J. H., Kim, K. N., Reznor, G., Dohlman, J. C., Langer, R., & Kohane, D. S. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1214634109 · Cited by 66
Influence of cooking method on the nutrient composition of Spanish light lamb
Campo, M. M., Muela, E., Olleta, J. L., et al. (2013) · Journal of Food Composition and Analysis · doi:10.1016/j.jfca.2013.05.010 · Cited by 68
The Importance of Rhamnolipid-Biosurfactant-Induced Changes in Bacterial Membrane Lipids of Bacillus subtilis for the Antimicrobial Activity of Thiosulfonates
Sotirova, A., Avramova, T., Stoitsova, S. R., Lazarkevich, I., Lubenets, V. I., Karpenko, E., & Galabova, D. (2012) · Current Microbiology · doi:10.1007/s00284-012-0191-7 · Cited by 70
Biosynthesis and analysis of plant oxylipins
Griffiths, G. J. (2014) · Free Radical Research · doi:10.3109/10715762.2014.1000318 · Cited by 69
Characterisation and antioxidant evaluation of Icelandic F. vesiculosus extracts in vitro and in fish-oil-enriched milk and mayonnaise
Hermund, D. B., Yeşiltaş, B., Honold, P. J., et al. (2015) · Journal of Functional Foods · doi:10.1016/j.jff.2015.02.020 · Cited by 68
Secretory phospholipase A 2 responsive liposomes
Zhu, G., Mock, J. N., Aljuffali, I. A., Cummings, B. S., & Arnold, R. D. (2011) · Journal of Pharmaceutical Sciences · doi:10.1002/jps.22530 · Cited by 68
The protective effect of resveratrol on islet insulin secretion and morphology in mice on a high-fat diet
Zhang, J., Chen, L., Zheng, J., Zeng, T., Li, H., Xiao, H., Deng, X., & Hu, X. (2012) · Diabetes Research and Clinical Practice · doi:10.1016/j.diabres.2012.02.029 · Cited by 70
Detection of a microbial consortium, including type II methanotrophs, by use of phospholipid fatty acids in an aerobic halogenated hydrocarbon-degrading soil column enriched with natural gas
Nichols, P. D., Antworth, C. P., Parsons, J., et al. (1987) · Environmental Toxicology and Chemistry · doi:10.1002/etc.5620060203 · Cited by 68
Loss of stearoyl-CoA desaturase 1 rescues cardiac function in obese leptin-deficient mice
Dobrzyń, P., Dobrzyń, A., Miyazaki, M., & Ntambi, J. M. (2010) · Journal of Lipid Research · doi:10.1194/jlr.m003780 · Cited by 69
Lipid and fatty acid composition of cytoplasmic membranes from Streptomyces hygroscopicus and its stable protoplast-type L form
Hoischen, C., Gura, K., Luge, C., & Gumpert, J. (1997) · Journal of Bacteriology · doi:10.1128/jb.179.11.3430-3436.1997 · Cited by 69
Novosphingobium panipatense sp. nov. and Novosphingobium mathurense sp. nov., from oil-contaminated soil
Gupta, S. K., Lal, D., & Lal, R. (2009) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.65743-0 · Cited by 68
Impact of natural (storm) and anthropogenic (trawling) sediment resuspension on particulate organic matter in coastal environments
Pusceddu, A., Grémare, A., Escoubeyrou, K., et al. (2005) · Continental Shelf Research · doi:10.1016/j.csr.2005.08.012 · Cited by 68
Cytokine secretion requires phosphatidylcholine synthesis
Tian, Y., Pate, C., Andreolotti, A. G., Wang, L., TUOMANEN, E. I., Boyd, K. L., Claro, E., & Jackowski, S. (2008) · The Journal of Cell Biology · doi:10.1083/jcb.200706152 · Cited by 67
Bile acid‐binding activity of young persimmon (Diospyros kaki) fruit and its hypolipidemic effect in mice
Matsumoto, K., Yokoyama, S., & Gato, N. (2009) · Phytotherapy Research · doi:10.1002/ptr.2911 · Cited by 68
Extracted fat from lamb meat by supercritical CO2 as feedstock for biodiesel production
Taher, H., Al‐Zuhair, S., AlMarzouqui, A., & Hashim, I. B. (2011) · Biochemical Engineering Journal · doi:10.1016/j.bej.2011.03.003 · Cited by 68
Potentially synbiotic fermented beverage with aqueous extracts of quinoa ( Chenopodium quinoa Willd) and soy
Bianchi, F., Rossi, E., Gomes, R., & Sivieri, K. (2014) · Food Science and Technology International · doi:10.1177/1082013214540672 · Cited by 70
Variation in Fatty Acid Composition of Artemia salina Nauplii Enriched with Microalgae and Baker's Yeast for Use in Larviculture
Chakraborty, R. D., Chakraborty, K., & Radhakrishnan, E. (2007) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf063654l · Cited by 68
Physiological ecology of juvenile chinook salmon (Oncorhynchus tshawytscha) at the southern end of their distribution, the San Francisco Estuary and Gulf of the Farallones, California*
MacFarlane, R. B., & Norton, E. C. (2002) · Digital Library Of The Commons Repository (Indiana University) · Cited by 68
Validation of a Rapid Measure of Blood PUFA Levels in Humans
Bailey‐Hall, E., NELSON, E. B., & Ryan, A. S. (2007) · Lipids · doi:10.1007/s11745-007-3140-7 · Cited by 68
Fatty acid and sterol composition of frozen and freeze-dried New Zealand Green Lipped Mussel (Perna canaliculus) from three sites in New Zealand.
Murphy, K. J., Mann, N. J., & Sinclair, A. J. (2003) · PubMed · Cited by 68
Changes in Membrane Lipid Composition during Saline Growth of the Fresh Water Cyanobacterium Synechococcus 6311
Huflejt, M. E., Trémolières, A., Pineau, B., et al. (1990) · PLANT PHYSIOLOGY · doi:10.1104/pp.94.4.1512 · Cited by 68
Formation and Characterization of Supported Lipid Bilayers Composed of Hydrogenated and Deuterated Escherichia coli Lipids
Lind, T. K., Wacklin, H. P., Schiller, J., et al. (2015) · PLoS ONE · doi:10.1371/journal.pone.0144671 · Cited by 68
Developmental Programming: Impact of Gestational Steroid and Metabolic Milieus on Adiposity and Insulin Sensitivity in Prenatal Testosterone-Treated Female Sheep
Cardoso, R. C., Veiga‐Lopez, A., Moeller, J., Beckett, E. M., Pease, A. P., Keller, E., Madrigal, V., Chazenbalk, G. D., Dumesic, D. A., & Padmanabhan, V. (2015) · Endocrinology · doi:10.1210/en.2015-1565 · Cited by 67
Production of biodiesel from freshwater microalgae and evaluation of fuel properties based on fatty acid methyl ester profile
Sinha, S. K., Gupta, A., & Bharalee, R. (2016) · Biofuels · doi:10.1080/17597269.2015.1118781 · Cited by 68
Sustainable production of bioethanol using lipid-extracted biomass from Scenedesmus dimorphus
Chng, L. M., Chan, D. J. C., & Lee, K. T. (2016) · Journal of Cleaner Production · doi:10.1016/j.jclepro.2016.02.016 · Cited by 68
Latitudinal variation in ecological opportunity and intraspecific competition indicates differences in niche variability and diet specialization of Arctic marine predators
Yurkowski, D. J., Ferguson, S. H., Choy, E. S., Loseto, L. L., Brown, T. M., Muir, D. C. G., Semeniuk, C. A. D., & Fisk, A. T. (2016) · Ecology and Evolution · doi:10.1002/ece3.1980 · Cited by 69
Vimentin affects localization and activity of sodium-glucose cotransporter SGLT1 in membrane rafts
Runembert, I., Queffeulou, G., Fédérici, P., et al. (2002) · Journal of Cell Science · doi:10.1242/jcs.115.4.713 · Cited by 68
Characterization and Screening of Native Scenedesmus sp. Isolates Suitable for Biofuel Feedstock
Gour, R. S., Chawla, A., Singh, H., Chauhan, R. S., & Kant, A. (2016) · PLoS ONE · doi:10.1371/journal.pone.0155321 · Cited by 68
Functional tea from a Brazilian berry: Overview of the bioactives compounds
da Silva-Maia, J. K., Batista, Â. G., Cazarin, C. B. B., Dionísio, A. P., de Brito, E. S., Biasoto, A. C. T., & Maróstica, M. R. (2016) · LWT · doi:10.1016/j.lwt.2016.06.016 · Cited by 69
Lactic Acid Bacteria Protects Caenorhabditis elegans from Toxicity of Graphene Oxide by Maintaining Normal Intestinal Permeability under different Genetic Backgrounds
Zhao, Y., Yu, X., Jia, R., et al. (2015) · Scientific Reports · doi:10.1038/srep17233 · Cited by 68
Enzymatic measurement of phosphatidylglycerol and cardiolipin in cultured cells and mitochondria
Morita, S., & Terada, T. (2015) · Scientific Reports · doi:10.1038/srep11737 · Cited by 67
Oxidative Damage to the Salivary Glands of Rats with Streptozotocin-Induced Diabetes-Temporal Study: Oxidative Stress and Diabetic Salivary Glands
Knaś, M., Maciejczyk, M., Daniszewska, I., Klimiuk, A., Matczuk, J., Kołodziej, U., Waszkiel, D., Ładny, J. R., Żendzian‐Piotrowska, M., & Zalewska, A. (2016) · Journal of Diabetes Research · doi:10.1155/2016/4583742 · Cited by 69
Influence of partial pork backfat replacement by fish oil on nutritional and technological properties of liver pâté
Domínguez, R., Pateiro, M., Munekata, P. E. S., et al. (2016) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.201600178 · Cited by 68
Urban wastewater treatment by Tetraselmis sp. CTP4 (Chlorophyta)
Schulze, P. S. C., de Carvalho, C. F. M., Pereira, H., Gangadhar, K. N., Schüler, L., Santos, T., Varela, J. C. S., & Barreira, L. A. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2016.10.027 · Cited by 75
Xeropreservation of functionalized lipid biomarkers in hyperarid soils in the Atacama Desert
Wilhelm, M. B., Dávila, A. F., Eigenbrode, J. L., Parenteau, M. N., Jahnke, L. L., Liu, X., Summons, R. E., Wray, J. J., Stamos, B. N., O’Reilly, S. S., et al. (2016) · Organic Geochemistry · doi:10.1016/j.orggeochem.2016.10.015 · Cited by 68
The Leukotriene B4 and its Receptor BLT1 Act as Critical Drivers of Neutrophil Recruitment in Murine Bullous Pemphigoid-Like Epidermolysis Bullosa Acquisita
Sezin, T., Krajewski, M. P., Wutkowski, A., Mousavi, S., Chakievska, L., Bieber, K., Ludwig, R. J., Dahlke, M., Rades, D., Schulze, F. S., et al. (2017) · Journal of Investigative Dermatology · doi:10.1016/j.jid.2016.12.021 · Cited by 68
Time-dependent effect of rutin on skin fibroblasts membrane disruption following UV radiation
Gęgotek, A., Bielawska, K., Biernacki, M., Dobrzyńska, I., & Skrzydlewska, E. (2017) · Redox Biology · doi:10.1016/j.redox.2017.04.014 · Cited by 69
Gut content analysis of Gammarus lacustris from a Siberian lake using biochemical and biophysical methods
Gladyshev, M. I., Emelianova, A., Kalachova, G. S., Zotina, T. A., Gaevsky, N. A., & Zhilenkov, M. D. (2000) · Hydrobiologia · doi:10.1023/a:1004036111433 · Cited by 67
Seasonal variation in nutritional composition and anti-proliferative activity of brown seaweed, Sargassum oligocystum
Praiboon, J., Palakas, S., Noiraksa, T., & Miyashita, K. (2017) · Journal of Applied Phycology · doi:10.1007/s10811-017-1248-6 · Cited by 68
Plasticity of energy reserves and metabolic performance of discus fish (Symphysodon aequifasciatus) exposed to low-temperature stress
Wen, B., Jin, S.-R., Chen, Z., et al. (2017) · Aquaculture · doi:10.1016/j.aquaculture.2017.09.002 · Cited by 68
Nutritional and protein quality of dry Brazilian beans (Phaseolus vulgaris L.)
Rezende, A. A., Pacheco, M. T. B., da Silva, V. S. N., et al. (2017) · Food Science and Technology · doi:10.1590/1678-457x.05917 · Cited by 68
Triglyceride profiling in adipose tissues from obese insulin sensitive, insulin resistant and type 2 diabetes mellitus individuals
Al‐Sulaiti, H., Diboun, I., Banu, S., Al-Emadi, M., Amani, P., Harvey, T. M., Dömling, A. S. S., Latiff, A. A., & Elrayess, M. A. (2018) · Journal of Translational Medicine · doi:10.1186/s12967-018-1548-x · Cited by 66
The occurrence of polyenoic very long chain fatty acids with greater than 32 carbon atoms in molecular species of phosphatidylcholine in normal and peroxisome-deficient (Zellweger's syndrome) brain
Poulos, A., Sharp, P. C., Johnson, D., & Easton, C. J. (1988) · Biochemical Journal · doi:10.1042/bj2530645 · Cited by 68
Effect of microencapsulated extract of pitaya (Hylocereus costaricensis) peel on color, texture and oxidative stability of refrigerated ground pork patties submitted to high pressure processing
Cunha, L. C. M., Monteiro, M. L. G., Costa-Lima, B. R. C., Guedes‐Oliveira, J. M., Alves, V. H. M., Almeida, A. L., Tonon, R. V., Rosenthal, A., & Conte‐Júnior, C. A. (2018) · Innovative Food Science & Emerging Technologies · doi:10.1016/j.ifset.2018.08.009 · Cited by 68
Diet and trophic niche space and overlap of Lake Ontario salmonid species using stable isotopes and stomach contents
Mumby, J. A., Larocque, S. M., Johnson, T. B., et al. (2018) · Journal of Great Lakes Research · doi:10.1016/j.jglr.2018.08.009 · Cited by 68
Stable Isotope Labeling Highlights Enhanced Fatty Acid and Lipid Metabolism in Human Acute Myeloid Leukemia
Stuani, L., Riols, F., Millard, P., Sabatier, M., Batut, A., Saland, E., Viars, F., Tonini, L., Zaghdoudi, S., Linares, L. K., et al. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19113325 · Cited by 72
Triauxic growth of an oleaginous red yeast Rhodosporidium toruloides on waste ‘extract’ for enhanced and concomitant lipid and β-carotene production
Singh, G., Sinha, S., Bandyopadhyay, K. K., Lawrence, M. L., & Paul, D. (2018) · Microbial Cell Factories · doi:10.1186/s12934-018-1026-4 · Cited by 68
Assessment of lipid oxidation in Atlantic mackerel (Scomber scombrus) subjected to different antioxidant and sous-vide cooking treatments by conventional and fluorescence microscopy methods
Cropotova, J., Mozūraitytė, R., Standal, I. B., & Rustad, T. (2019) · Food Control · doi:10.1016/j.foodcont.2019.04.016 · Cited by 68
Lipids detection and quantification in oleaginous microorganisms: an overview of the current state of the art
Patel, A., Antonopoulou, I., Enman, J., Rova, U., Christakopoulos, P., & Μάτσακας, Λ. (2019) · BMC Chemical Engineering · doi:10.1186/s42480-019-0013-9 · Cited by 71
Effects of Elevated Carbon Dioxide and Nitrogen Fertilization on Mycorrhizal Fine Roots and the Soil Microbial Community in Beech-Spruce Ecosystems on Siliceous and Calcareous Soil
Wiemken, V., Laczkó, E., Ineichen, K., et al. (2001) · Microbial Ecology · doi:10.1007/s002480000080 · Cited by 68
Synergistic Interaction between Sphingomyelin and Gemcitabine Potentiates Ceramide-Mediated Apoptosis in Pancreatic Cancer
Modrak, D. E., Cardillo, T. M., Newsome, G., et al. (2004) · Cancer Research · doi:10.1158/0008-5472.can-04-2988 · Cited by 68
Trophic interactions of Antarctic seals as determined by stable isotope signatures
Zhao, L., Castellini, M. A., Mau, T. L., et al. (2004) · Polar Biology · doi:10.1007/s00300-004-0598-0 · Cited by 68
Gelation of Proteins from Washed Muscle of Threadfin Bream ( Nemipterus japonicus ) under Mild Acidic Conditions
Chawla, S. P., Venugopal, V., & Nair, P. M. (1996) · Journal of Food Science · doi:10.1111/j.1365-2621.1996.tb14194.x · Cited by 68
Proximate composition of Antarctic mesopelagic fishes
Donnelly, J. L., Torres, J. J., Hopkins, T. L., & Lancraft, T. M. (1990) · Marine Biology · doi:10.1007/bf02114670 · Cited by 68
Reduction in hepatic cytochrome P-450 is correlated to the degree of liver fat content in animal models of steatosis in the absence of inflammation
Leclercq, I. A., Horsmans, Y., Desager, J., et al. (1998) · Journal of Hepatology · doi:10.1016/s0168-8278(98)80314-0 · Cited by 68
Biochemical Characterization of Pre-β1 High-Density Lipoprotein from Human Ovarian Follicular Fluid: Evidence for the Presence of a Lipid Core,
Jaspard‐Vinassa, B., Collet, X., Barbaras, R., et al. (1996) · Biochemistry · doi:10.1021/bi950938i · Cited by 68
c-Jun Is a Downstream Target for Ceramide-activated Protein Phosphatase in A431 Cells
Reyes, J. G., González, I., Delgado, P. S., González, I., Aguiar, J. Q., Estévez, F., Fanjul, L. F., & de Galarreta, C. M. R. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.35.21375 · Cited by 68
Peritubular Dentin, a Vertebrate Apatitic Mineralized Tissue without Collagen: Role of a Phospholipid-Proteolipid Complex
Gotliv, B., & Veis, A. (2007) · Calcified Tissue International · doi:10.1007/s00223-007-9053-x · Cited by 68
Lipid and fatty acid composition and energy partitioning during embryo development in the shrimp Macrobrachium borellii
Heras, H., González-Baró, M. R., & Pollero, R. J. (2000) · Lipids · doi:10.1007/s11745-000-0569-z · Cited by 68
Choline deficiency induces apoptosis in primary cultures of fetal neurons
Yen, C. E., Mar, M.-H. H., Meeker, R. B., Fernandes, A., & Zeisel, S. H. (2001) · The FASEB Journal · doi:10.1096/fj.00-0800com · Cited by 68
Effect of Overfeeding During the Dry Period on the Rate of Esterification in Adipose Tissue of Dairy Cows During the Periparturient Period
Rukkwamsuk, T., Wensing, T., & Geelen, M. J. H. (1999) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(99)75339-7 · Cited by 68
The Role and Mechanism of Diacylglycerol-Protein Kinase C1 Signaling in Melanogenesis by Cryptococcus neoformans
Heung, L. J., Kaiser, A. E., Luberto, C., & Poeta, M. D. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m503404200 · Cited by 68
Biochemical composition and energy allocation in the tropical scallop Lyropecten (Nodipecten) nodosus during the months leading up to and following the development of gonads
Lodeiros, C. J., Rengel, J., Guderley, H., Nusetti, O., & Himmelman, J. H. (2001) · Aquaculture · doi:10.1016/s0044-8486(01)00505-1 · Cited by 68
Production of arachidonic acid byMortierella alpina ATCC 32222
Bajpai, P. K., Bajpai, P., & Ward, O. P. (1991) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/bf01575851 · Cited by 68
Diet/management effects on carcass attributes and meat quality of young goats
Johnson, D. D., & McGowan, C. (1998) · Small Ruminant Research · doi:10.1016/s0921-4488(97)00071-0 · Cited by 68
Immunosuppressive effects of polyunsaturated fatty acids on antigen presentation by human leukocyte antigen class I molecules
Shaikh, S. R., & Edidin, M. A. (2006) · Journal of Lipid Research · doi:10.1194/jlr.m600365-jlr200 · Cited by 68
Comparison between methods using low-toxicity solvents for the extraction of lipids from herring (Clupea harengus)
Undeland, I., Härröd, M., & Lingnert, H. (1998) · Food Chemistry · doi:10.1016/s0308-8146(97)00053-8 · Cited by 68

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks