Cited by 54,751Lipid metabolism and biosynthesisEdible Oils Quality and AnalysisFood Nutrient Composition and Product Development

The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Bligh, E. G., Dyer, W. J.·1959·Canadian Journal of Biochemistry and Physiology

DOI 10.1139/o59-099

Open this paper in CoChat — free account

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

Integrity signals

Registry

Crossref · 10.1139/o59-099

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Storage of Frozen Plaice Fillets
Dyer, W. J., & Morton, M. L. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-007 · Cited by 69
Self-citation
Experimental Determination of Binodal Curves, Plait Points, and Tie Lines, in 50 Systems, each consisting of Water and Two Organic Liquids
Bonner, W. D. (1910) · The Journal of Physical Chemistry · doi:10.1021/j150116a004 · Cited by 46
Acetone–Water Mixtures for the Extraction and Rapid Estimation of Fats of Biological Materials, Particularly Fish Products
Dambergs, N. (1956) · Journal of the Fisheries Research Board of Canada · doi:10.1139/f56-045 · Cited by 18

Show all 5 references

Research works printed but not linked: 1

BANCROFT W., 1895, Phys. Iiev., 3, 1

Sorted by citations

Items 9,301–9,400 of 48,846

Purification and properties of cytochrome b556 in the respiratory chain of aerobically grown Escherichia coli K12.
Kita, K., Yamato, I., & Anraku, Y. (1978) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34264-3 · Cited by 67
The role of lipids in the activity of adenylate cyclase of rat liver plasma membranes
Réthy, A., Tomasi, V., & Trevisani, A. (1971) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(71)90306-7 · Cited by 67
Phospholipase A activity associated with membranes of human polymorphonuclear leucocytes
Franson, R., Weiss, J. P., Martin, L. T., et al. (1977) · Biochemical Journal · doi:10.1042/bj1670839 · Cited by 67
[14] Phosphatidylcholine exchange protein from beef liver
Kamp, H. H., & WIRTZ, K. W. A. (1974) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(74)32017-4 · Cited by 67
Evidence for production of 1-acyl-2-acetyl-sn-glyceryl-3-phosphorylcholine concomitantly with platelet-activating factor
Satouchi, K., Oda, M., Yasunaga, K., et al. (1985) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(85)91097-6 · Cited by 67
Protection from inactivation of the adenine nucleotide translocator during hypoglycaemia-induced apoptosis by mitochondrial phospholipid hydroperoxide glutathione peroxidase
IMAI, H., KOUMURA, T., NAKAJIMA, R., NOMURA, K., & NAKAGAWA, Y. (2003) · Biochemical Journal · doi:10.1042/bj20021342 · Cited by 67
Docosatetraenoyl LPA is elevated in exhaled breath condensate in idiopathic pulmonary fibrosis
Montesi, S. B., Mathai, S. K., Brenner, L. N., Gorshkova, I. A., Berdyshev, E. V., Tager, A. M., & Shea, B. S. (2014) · BMC Pulmonary Medicine · doi:10.1186/1471-2466-14-5 · Cited by 66
Production and partial purification of γ-linolenic acid and some pigments fromSpirulina platensis
Cohen, Z., Reungjitchachawali, M., Siangdung, W., & Tanticharoen, M. (1993) · Journal of Applied Phycology · doi:10.1007/bf02182428 · Cited by 66
Alpha linolenic acid (ALA) from Rosa canina, sacha inchi and chia oils may increase ALA accretion and its conversion into n-3 LCPUFA in diverse tissues of the rat
Valenzuela B, R., Barrera R, C., González-Astorga, M., Sanhueza C, J., & Valenzuela B, A. (2014) · Food Funct. · doi:10.1039/c3fo60688k · Cited by 65
CO2 recycling using microalgae for the production of fuels
Wilson, M. H., Groppo, J., Placido, A., Graham, S., Morton, S. A., Santillan-Jimenez, E., Shea, A., Crocker, M., Crofcheck, C., & Andrews, R. (2014) · Applied Petrochemical Research · doi:10.1007/s13203-014-0052-3 · Cited by 66
Detection of phospholipid oxidation in oxidatively stressed cells by reversed-phase HPLC coupled with positive-ionization electroscopy MS
Spickett, C. M., RENNIE, N., WINTER, H., et al. (2001) · Biochemical Journal · doi:10.1042/0264-6021:3550449 · Cited by 66
Separation of isomeric lysophospholipids by reverse phase HPLC
Creer, M. H., & Gross, R. W. (1985) · Lipids · doi:10.1007/bf02534778 · Cited by 66
Different Impacts of Short-Chain Fatty Acids on Saturated and Polyunsaturated Fatty Acid Biosynthesis in Aurantiochytrium sp. SD116
Song, X., Tan, Y., Liu, Y., Zhang, J., Liu, G., Feng, Y., & Cui, Q. (2013) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf403153p · Cited by 65
The Good, the Bad, and the Toxic: Approaching Hormesis in Daphnia magna Exposed to an Energetic Compound
Stanley, J. K., Perkins, E. J., Habib, T., Sims, J. G., Chappell, P., Escalon, B. L., Wilbanks, M. S., & García‐Reyero, N. (2013) · Environmental Science & Technology · doi:10.1021/es401115q · Cited by 66
Overexpression of the Adiponectin Receptor AdipoR1 in Rat Skeletal Muscle Amplifies Local Insulin Sensitivity
Patel, S. A., Hoehn, K. L., Lawrence, R., Sawbridge, L., Talbot, N. A., Tomsig, J. L., Turner, N. A., Cooney, G. J., Whitehead, J. P., Kraegen, E. W., et al. (2012) · Endocrinology · doi:10.1210/en.2012-1368 · Cited by 66
Blubber fatty acid profiles indicate dietary resource partitioning between adult and juvenile southern elephant seals
Newland, C., Field, I., Nichols, P., et al. (2009) · Marine Ecology Progress Series · doi:10.3354/meps08010 · Cited by 66
Resveratrol and fenofibrate ameliorate fructose-induced nonalcoholic steatohepatitis by modulation of genes expression
El-Haleim, E. A. A., Bahgat, A. K., & Saleh, S. (2016) · World Journal of Gastroenterology · doi:10.3748/wjg.v22.i10.2931 · Cited by 68
Production of Chitin from Penaeus vannamei By-Products to Pilot Plant Scale Using a Combination of Enzymatic and Chemical Processes and Subsequent Optimization of the Chemical Production of Chitosan by Response Surface Methodology
Vázquez, J. A., Ramos, P., Mirón, J., et al. (2017) · Marine Drugs · doi:10.3390/md15060180 · Cited by 66
Lipid extraction from microalgae covered with biomineralized cell walls using liquefied dimethyl ether
Kanda, H., Hoshino, R., Murakami, K., Diono, W., Zheng, Q., & Goto, M. (2019) · Fuel · doi:10.1016/j.fuel.2019.116590 · Cited by 67
Promoting Chlorella photosynthesis and bioresource production using directionally prepared carbon dots with tunable emission
Xue, R., Fu, L., Dong, S., Yang, H., & Zhou, D. (2020) · Journal of Colloid and Interface Science · doi:10.1016/j.jcis.2020.02.080 · Cited by 64
Inulin alleviates adverse metabolic syndrome and regulates intestinal microbiota composition in Nile tilapia (Oreochromis niloticus) fed with high-carbohydrate diet
Wang, T., Zhang, N., Yu, X., et al. (2020) · British Journal Of Nutrition · doi:10.1017/s000711452000402x · Cited by 66
“Total lipid in whole fish body and in liver was calculated by a classic methanol – chloroform method (23) .”
Improvement of carotenoids production from Rhodotorula glutinis CCT-2186
Mussagy, C. U., Guimarães, A. A. C., Rocha, L. V. F., et al. (2020) · Biochemical Engineering Journal · doi:10.1016/j.bej.2020.107827 · Cited by 66
Cultivation of Nostoc sp. LS04 in municipal wastewater for biodiesel production and their deoiled biomass cellular extracts as biostimulants for Lactuca sativa growth improvement
Silambarasan, S., Logeswari, P., Sivaramakrishnan, R., Kamaraj, B., Lan, N. T., & Cornejo, P. (2021) · Chemosphere · doi:10.1016/j.chemosphere.2021.130644 · Cited by 65
Prochlorococcus extracellular vesicles: molecular composition and adsorption to diverse microbes
Biller, S. J., Lundeen, R. A., Hmelo, L. R., Becker, K. W., Arellano, A. A., Dooley, K. D., Heal, K. R., Carlson, L. T., Mooy, B. A. S. V., Ingalls, A. E., et al. (2021) · Environmental Microbiology · doi:10.1111/1462-2920.15834 · Cited by 64
“…pellets and vesicles originating from triplicate 20 l batch cultures of Prochlorococcus MIT9312 or MIT9313 were extracted using a modi ﬁ ed Bligh and Dyer technique with cold 1:1 methanol/water (aqueous phase) and cold dichloromethane (organic phase) (Bligh and Dyer, 1959; Boysen et al ., 2018).”
Skeletal muscle undergoes fiber type metabolic switch without myosin heavy chain switch in response to defective fatty acid oxidation
Pereyra, A. S., Lin, C., Sanchez, D. M., Laskin, J., Spangenburg, E. E., Neufer, P. D., Fisher‐Wellman, K. H., & Ellis, J. M. (2022) · Molecular Metabolism · doi:10.1016/j.molmet.2022.101456 · Cited by 66
“Yield was assessed by protein concentration using the BCA method [13].”
Method
Chronic Lactate Exposure Decreases Mitochondrial Function by Inhibition of Fatty Acid Uptake and Cardiolipin Alterations in Neonatal Rat Cardiomyocytes
San-Millán, I., Sparagna, G. C., Chapman, H. L., Warkins, V. L., Chatfield, K. C., Shuff, S. R., Martinez, J. L., & Brooks, G. A. (2022) · Frontiers in Nutrition · doi:10.3389/fnut.2022.809485 · Cited by 64
DIEL VARIATIONS OF CARBOHYDRATES AND NEUTRAL LIPIDS IN NITROGEN‐SUFFICIENT AND NITROGEN‐STARVED CYCLOSTAT CULTURES OF ISOCHRYSIS SP.1
Lacour, T., Sciandra, A., Talec, A., Mayzaud, P., & Olivier Bernard, A. (2012) · Journal of Phycology · doi:10.1111/j.1529-8817.2012.01177.x · Cited by 69
A somatic cell mutant defective in phosphatidylglycerophosphate synthase, with impaired phosphatidylglycerol and cardiolipin biosynthesis.
Ohtsuka, T., Nishijima, M., & Akamatsu, Y. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)41612-2 · Cited by 66
Role of disulfide bonds in maintaining the structural integrity of the sheath of Leptothrix discophora SP-6
Emerson, D., & Ghiorse, W. C. (1993) · Journal of Bacteriology · doi:10.1128/jb.175.24.7819-7827.1993 · Cited by 66
Role of the satiety factor oleoylethanolamide in alcoholism
Bilbao, A., Serrano, A., Cippitelli, A., Pavón, F. J., Giuffrida, A., Suárez, J., García‐Marchena, N., Baixerás, E., de Heras, R. G., Orío, L., et al. (2015) · Addiction Biology · doi:10.1111/adb.12276 · Cited by 67
Segregation of Saturated Chain Lipids in Pulmonary Surfactant Filmsand Bilayers
Nag, K., Pao, J., Harbottle, R. R., et al. (2002) · Biophysical Journal · doi:10.1016/s0006-3495(02)75552-5 · Cited by 66
Characterisation of a potential biomarker of phospholipidosis from amiodarone-treated rats
Mortuza, G. B., Neville, W. A., Delaney, J., Waterfield, C. J., & Camilleri, P. (2003) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/s1388-1981(02)00361-x · Cited by 65
Allosteric transitions of Torpedo acetylcholine receptor in lipids, detergent and amphipols: molecular interactions vs. physical constraints
Martinez, K. L., Gohon, Y., Corringer, P., et al. (2002) · FEBS Letters · doi:10.1016/s0014-5793(02)03306-9 · Cited by 66
Fish crackers development from minced fish and starch: an innovative approach to a traditional product
Neiva, C. R. P., Machado, T. M., Tomita, R. Y., et al. (2011) · Food Science and Technology · doi:10.1590/s0101-20612011000400024 · Cited by 66
Comprehensive Imaging of Porcine Adrenal Gland Lipids by MALDI-FTMS Using Quercetin as a Matrix
Wang, X., Han, J., Pan, J., & Borchers, C. H. (2013) · Analytical Chemistry · doi:10.1021/ac404044k · Cited by 68
Determination of endocannabinoids in nematodes and human brain tissue by liquid chromatography electrospray ionization tandem mass spectrometry
Lehtonen, M., Storvik, M., Malinen, H., Hyytiä, P., Lakso, M., Auriola, S. O. K., Wong, G., & Callaway, J. C. (2011) · Journal of Chromatography B · doi:10.1016/j.jchromb.2011.02.004 · Cited by 66
Implications of oxidative stress in the cytotoxicity of Pseudomonas aeruginosa ExoU
Saliba, A. M., Assis, M.-C., Nishi, R., et al. (2005) · Microbes and Infection · doi:10.1016/j.micinf.2005.07.011 · Cited by 66
Physicochemical and microbiological effects of long- and short-term winery wastewater application to soils
Mosse, K. P. M., Patti, A. F., Smernik, R. J., et al. (2011) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2011.11.071 · Cited by 66
Effects of growing region and maturity stages on oil yield and fatty acid composition of coriander (Coriandrum sativum L.) fruit
Msaâda, K., Hosni, K., Taârit, M. B., et al. (2009) · Scientia Horticulturae · doi:10.1016/j.scienta.2008.11.033 · Cited by 66
Phytosterols can impair vitamin D intestinal absorption in vitro and in mice
Goncalves, A., Gleize, B., Bott, R., Nowicki, M., Amiot, M. J., Lairon, D., Borel, P., & Reboul, E. (2011) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.201100055 · Cited by 66
Single run HPLC separation coupled to evaporative light scattering detection unravels wheat flour endogenous lipid redistribution during bread dough making
Gerits, L. R., Pareyt, B., & Delcour, J. A. (2013) · LWT · doi:10.1016/j.lwt.2013.03.015 · Cited by 66
Effects of fish oil type, lipid antioxidants and presence of rapeseed oil on oxidative flavour stability of fish oil enriched milk
Let, M. B., Jacobsen, C., & Meyer, A. S. (2004) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.200300901 · Cited by 65
Benchmarking value in the pork supply chain: Processing characteristics and consumer evaluations of pork bellies of different thicknesses when manufactured into bacon
Person, R. C., McKenna, D. R., Griffin, D. B., et al. (2005) · Meat Science · doi:10.1016/j.meatsci.2004.12.012 · Cited by 66
Vegetable oil blends with α-linolenic acid rich Garden cress oil modulate lipid metabolism in experimental rats
Umesha, S. S., & Naidu, K. A. (2012) · Food Chemistry · doi:10.1016/j.foodchem.2012.05.118 · Cited by 66
The production of biofuel and bioelectricity associated with wastewater treatment by green algae
Maity, J. P., Hou, C.-P., Majumder, D., Bhattacharya, P., Kulp, T. R., Chen, C., Chuang, L.-T., Chen, C. N., Jean, J., Yang, T., et al. (2014) · Energy · doi:10.1016/j.energy.2014.06.023 · Cited by 66
Microbial signature lipid biomarker analysis - an approach that is still preferred, even amid various method modifications
Willers, C., van Rensburg, P. J., & Claassens, S. (2015) · Journal of Applied Microbiology · doi:10.1111/jam.12798 · Cited by 66
Discriminating faecal pollution: A case study of stormwater entering Port Phillip Bay, Australia
(1998) · Water Science & Technology · doi:10.1016/s0273-1223(98)00728-8 · Cited by 66
Feeding preferences of the sandhopper Orchestoidea tuberculata: the importance of algal traits
Duarte, C., Navarro, J. M., Acuña, K., et al. (2010) · Hydrobiologia · doi:10.1007/s10750-010-0309-5 · Cited by 66
Lipid and fatty acid composition of holothurians from tropical and temperate waters
Svetashev, V. I., Levin, V. S., Lam, C. N., et al. (1991) · Comparative Biochemistry and Physiology Part B Comparative Biochemistry · doi:10.1016/0305-0491(91)90242-6 · Cited by 66
Impacts of epigeic, anecic and endogeic earthworms on metal and metalloid mobility and availability
Sizmur, T. P., Tilston, E. L., Charnock, J., et al. (2010) · Journal of Environmental Monitoring · doi:10.1039/c0em00519c · Cited by 66
The Impact of Nutritional Fatty Acids during Pregnancy and Lactation on Early Human Adipose Tissue Development
Hauner, H., Vollhardt, C., Schneider, K. T. M., Zimmermann, A., Schuster, T., & Amann‐Gassner, U. (2009) · Annals of Nutrition and Metabolism · doi:10.1159/000209267 · Cited by 66
Opposing Effects of Omega-3 and Omega-6 Long Chain Polyunsaturated Fatty Acids on the Expression of Lipogenic Genes in Omental and Retroperitoneal Adipose Depots in the Rat
Mühlhäusler, B. S., Cook‐Johnson, R. J., James, M. J., Miljkovic, D., Duthoit, E., & Gibson, R. A. (2010) · Journal of Nutrition and Metabolism · doi:10.1155/2010/927836 · Cited by 70
Effects of culture duration on hydrogen peroxide-induced hepatocyte toxicity
Ruch, R. J., Crist, K. A., & Klaunig, J. E. (1989) · Toxicology and Applied Pharmacology · doi:10.1016/0041-008x(89)90293-7 · Cited by 66
Temporal changes in tissue 1α,25-dihydroxyvitamin D3, vitamin D receptor target genes, and calcium and PTH levels after 1,25(OH)2D3treatment in mice
Chow, E. C. Y., Quach, H. P., Vieth, R., & Pang, K. S. (2013) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00489.2012 · Cited by 65
Differences between Bligh and Dyer and Soxhlet extractions of PCBs and lipids from fat and lean fish muscle: Implications for data evaluation
Ewald, G., Bremle, G., & Karlsson, A. (1998) · Marine Pollution Bulletin · doi:10.1016/s0025-326x(97)00176-8 · Cited by 66
Modulation of indomethacin‐induced gastric injury by spermine and taurine in rats
Motawi, T. K., Abdelgawad, H. M., & Shahin, N. N. (2007) · Journal of Biochemical and Molecular Toxicology · doi:10.1002/jbt.20194 · Cited by 65
Characterization of Tafazzin Splice Variants from Humans and Fruit Flies
Xu, Y., Zhang, S., Malhotra, A., Edelman‐Novemsky, I., Ma, J., Kruppa, A. J., Cernicica, C., Blais, S. P., Neubert, T. A., Ren, M., et al. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.016642 · Cited by 66
Increased Elongase and Desaturase Gene Expression with Stearidonic Acid Enriched Diet Does Not Enhance Long-Chain (n-3) Content of Seawater Atlantic Salmon (Salmo salar L.)
Miller, M. R., Bridle, A. R., Nichols, P. D., et al. (2008) · Journal of Nutrition · doi:10.3945/jn.108.091702 · Cited by 66
Biophysical inhibition of synthetic vs. naturally-derived pulmonary surfactant preparations by polymeric nanoparticles
Beck‐Broichsitter, M., Ruppert, C., Schmehl, T., Günther, A., & Seeger, W. (2013) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2013.10.016 · Cited by 65
Isolation and characterization of lipid in phloem sap of canola
Madey, E., Nowack, L. M., & Thompson, J. E. (2002) · Planta · doi:10.1007/s004250100649 · Cited by 66
Cystathionine beta-synthase null homocystinuric mice fail to exhibit altered hemostasis or lowering of plasma homocysteine in response to betaine treatment
Maclean, K. N., Sikora, J., Kožich, V., Jiang, H., Greiner, L. S., Kraus, E., Krijt, J., Crnic, L. S., Allen, R. H., Stabler, S. P., et al. (2010) · Molecular Genetics and Metabolism · doi:10.1016/j.ymgme.2010.06.007 · Cited by 66
Fatty Aldehydes in Cyanobacteria Are a Metabolically Flexible Precursor for a Diversity of Biofuel Products
Kaiser, B. K., Carleton, M. O., Hickman, J. W., Miller, C. R., Lawson, D., Budde, M. W., Warrener, P., Paredes, A. M., Mullapudi, S., Navarro, P., et al. (2013) · PLoS ONE · doi:10.1371/journal.pone.0058307 · Cited by 65
Low-density lipoproteins isolated from the blood of patients with coronary heart disease induce the accumulation of lipids in human aortic cells
Tertov, V. V., Orekhov, A. N., Martsenyuk, O. N., et al. (1989) · Experimental and Molecular Pathology · doi:10.1016/0014-4800(89)90043-9 · Cited by 66
Interaction of ostreolysin, a cytolytic protein from the edible mushroom Pleurotus ostreatus, with lipid membranes and modulation by lysophospholipids
Sepčić, K., Berne, S., Potrich, C., et al. (2003) · European Journal of Biochemistry · doi:10.1046/j.1432-1033.2003.03480.x · Cited by 66
Fatty acid profile and mineral content of the wild snail (Helix pomatia) from the region of the south of the Turkey
Özoğul, Y., Özoğul, F., & Olgunoglu, A. I. (2005) · European Food Research and Technology · doi:10.1007/s00217-005-1191-7 · Cited by 69
Temporal and seasonal change in microbial community structure of an undisturbed, disturbed, and carbon-amended pasture soil
Stevenson, B., Hunter, D., & Rhodes, P. L. (2014) · Soil Biology and Biochemistry · doi:10.1016/j.soilbio.2014.04.010 · Cited by 66
Autotrophy versus heterotrophy: The origin of carbon determines its fate in a symbiotic sea anemone
Bachar, A., Achituv, Y., Pasternak, Z., et al. (2007) · Journal of Experimental Marine Biology and Ecology · doi:10.1016/j.jembe.2007.05.030 · Cited by 66
Assessment of anti-diabetic activity of an ethnopharmacological plant Nerium oleander through alloxan induced diabetes in mice
Dey, P., Saha, M. R., Chowdhuri, S. R., et al. (2014) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2014.12.012 · Cited by 66
Effects of inhibitory compounds in lignocellulosic hydrolysates on Mortierella isabellina growth and carbon utilization
Ruan, Z., Hollinshead, W. D., Isaguirre, C., Tang, Y., Liao, W., & Liu, Y. (2015) · Bioresource Technology · doi:10.1016/j.biortech.2015.02.026 · Cited by 67
Gbb/BMP signaling is required to maintain energy homeostasis in Drosophila
Ballard, S. L., Jarolimova, J., & Wharton, K. A. (2009) · Developmental Biology · doi:10.1016/j.ydbio.2009.11.011 · Cited by 67
Hypocholesterolaemic activity of 3-hydroxy-3-methyl-glutaryl flavanones enriched fraction from bergamot fruit (Citrus bergamia): “In vivo” studies
Donna, L. D., Iacopetta, D., Cappello, A. R., Gallucci, G., Martello, E., Fiorillo, M., Dolce, V., & Sindona, G. (2014) · Journal of Functional Foods · doi:10.1016/j.jff.2013.12.029 · Cited by 66
The Saccharomyces cerevisiae Actin Patch Protein App1p Is a Phosphatidate Phosphatase Enzyme
Chae, M., Han, G., & Carman, G. M. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.421776 · Cited by 66
Pressure effects on the composition and thermal behavior of lipids from the deep-sea thermophile Methanococcus jannaschii
Kaneshiro, S. M., & Clark, D. S. (1995) · Journal of Bacteriology · doi:10.1128/jb.177.13.3668-3672.1995 · Cited by 66
Identification of N-Acetyltaurine as a Novel Metabolite of Ethanol through Metabolomics-guided Biochemical Analysis
Shi, X., Yao, D., & Chen, C. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.312199 · Cited by 65
Identification and Isolation of a Castellaniella Species Important during Biostimulation of an Acidic Nitrate- and Uranium-Contaminated Aquifer
Spain, A. M., Peacock, A. D., Istok, J. D., Elshahed, M. S., Najar, F. Z., Roe, B. A., White, D. C., & Krumholz, L. R. (2007) · Applied and Environmental Microbiology · doi:10.1128/aem.00331-07 · Cited by 66
Regulation of Cell Migration by Sphingomyelin Synthases: Sphingomyelin in Lipid Rafts Decreases Responsiveness to Signaling by the CXCL12/CXCR4 Pathway
Asano, S., Kitatani, K., Taniguchi, M., et al. (2012) · Molecular and Cellular Biology · doi:10.1128/mcb.00121-12 · Cited by 66
Accumulation of nonesterified fatty acids causes the sustained energetic deficit in kidney proximal tubules after hypoxia-reoxygenation
Feldkamp, T., Kribben, A., Roeser, N. F., Senter, R. A., & Weinberg, J. M. (2005) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.00305.2005 · Cited by 64
Determination of the urinary aglycone metabolites of vitamin K by HPLC with redox-mode electrochemical detection
Harrington, D. J., Soper, R., Edwards, C., Savidge, G. F., Hodges, S. J., & Shearer, M. J. (2005) · Journal of Lipid Research · doi:10.1194/jlr.d400033-jlr200 · Cited by 66
Schistosomal‐Derived Lysophosphatidylcholine Are Involved in Eosinophil Activation and Recruitment through Toll‐Like Receptor–2–Dependent Mechanisms
Magalhães, K. G., de Almeida, P. E., Atella, G. C., Maya‐Monteiro, C. M., Castro-Faria-Neto, H. C., Pelajo‐Machado, M., Lenzi, H. L., Bozza, M. T., & Bozza, P. T. (2010) · The Journal of Infectious Diseases · doi:10.1086/656477 · Cited by 67
Isoprostane Measurement in Plasma and Urine by Liquid Chromatography–Mass Spectrometry with One-Step Sample Preparation
Sircar, D., & Subbaiah, P. V. (2007) · Clinical Chemistry · doi:10.1373/clinchem.2006.074989 · Cited by 67
BIOACTIVE POLAR LIPIDS IN OLIVE OIL, POMACE AND WASTE BYPRODUCTS
Karantonis, H. C., Tsantila, N., Stamatakis, G. M., Samiotaki, M. K., Panayotou, G., Antonopoulou, S., & Demopoulos, C. A. (2008) · Journal of Food Biochemistry · doi:10.1111/j.1745-4514.2008.00160.x · Cited by 65
Docosahexaenoic acid in plasma phosphatidylcholine may be a potential marker for in vivo phosphatidylethanolamine N-methyltransferase activity in humans
da Costa, K.-A., Sanders, L., Fischer, L. M., et al. (2011) · American Journal of Clinical Nutrition · doi:10.3945/ajcn.110.011064 · Cited by 66
Natural variation of bovine milk fat globule size within a herd
Logan, A., Auldist, M. J., Greenwood, J. S., et al. (2014) · Journal of Dairy Science · doi:10.3168/jds.2014-8010 · Cited by 66
Quality traits in muscle biceps femoris and back-fat from purebred Iberian and reciprocal Iberian×Duroc crossbred pigs
Ventanas, S., Ventanas, J., Jurado, Á., et al. (2006) · Meat Science · doi:10.1016/j.meatsci.2006.03.009 · Cited by 66
Fibrillin 5 Is Essential for Plastoquinone-9 Biosynthesis by Binding to Solanesyl Diphosphate Synthases in Arabidopsis
Kim, E.-H., Lee, Y.-J., & Kim, H. U. (2015) · The Plant Cell · doi:10.1105/tpc.15.00707 · Cited by 66
Growth, biochemical profile, and fatty acid composition of mussel (Mytilus galloprovincialis Lmk.) cultured in the open ocean of the Bay of Biscay (northern Spain)
Azpeitia, K., Ferrer, L., Revilla, M., et al. (2015) · Aquaculture · doi:10.1016/j.aquaculture.2015.12.022 · Cited by 66
Hyaluronan-conjugated liposomes encapsulating gemcitabine for breast cancer stem cells
Han, Shin, D. H., Kim, J. S., Weon, K. Y., Jang, C.-Y., & Kim, J.-S. (2016) · International Journal of Nanomedicine · doi:10.2147/ijn.s95850 · Cited by 65
Method Development for Fecal Lipidomics Profiling
Gregory, K. E., Bird, S. S., Gross, V., et al. (2012) · Analytical Chemistry · doi:10.1021/ac303011k · Cited by 66
Remodeling of lipid bodies by docosahexaenoic acid in activated microglial cells
Tremblay, M.-E., Zhang, I., Bisht, K., Savage, J. C., Lecours, C., Parent, M., Titorenko, V., & Maysinger, D. (2016) · Journal of Neuroinflammation · doi:10.1186/s12974-016-0580-0 · Cited by 66
Mast Cell Degranulation Is Accompanied by the Release of a Selective Subset of Extracellular Vesicles That Contain Mast Cell–Specific Proteases
Kormelink, T. G., Arkesteijn, G. J. A., van de Lest, C. H. A., Geerts, W. J. C., Goerdayal, S. S., Altelaar, M. A. F., Redegeld, F. A., Hoen, E. N. M. N., & Wauben, M. H. M. (2016) · The Journal of Immunology · doi:10.4049/jimmunol.1600614 · Cited by 67
Yield, composition, and antioxidant activity of avocado pulp oil extracted by pressurized fluids
ABAIDE, E. R., Zabot, G. L., Tres, M. V., Martins, R. F., Fagundez, J. L. S., Nunes, L. F., Druzian, S. P., Soares, J. F., Prá, V. D., Silva, J. R. F., et al. (2017) · Food and Bioproducts Processing · doi:10.1016/j.fbp.2017.01.008 · Cited by 66
Region-specific vulnerability to lipid peroxidation and evidence of neuronal mechanisms for polyunsaturated fatty acid biosynthesis in the healthy adult human central nervous system
Naudí, A., Cabré, R., Domínguez-González, M., Ayala, V., Jové, M., Mota‐Martorell, N., Piñol‐Ripoll, G., Gil‐Villar, M. P., Rué, M., Portero‐Otín, M., et al. (2017) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2017.02.001 · Cited by 64
Effects of epigallocatechin-3-gallate on thermogenesis and mitochondrial biogenesis in brown adipose tissues of diet-induced obese mice
Lee, M.-S., Shin, Y., Jung, S., & Kim, Y. (2017) · Food & Nutrition Research · doi:10.1080/16546628.2017.1325307 · Cited by 66
A Fluorescence Polarization Activity-Based Protein Profiling Assay in the Discovery of Potent, Selective Inhibitors for Human Nonlysosomal Glucosylceramidase
Lahav, D., Liu, B., van den Berg, R. J. B. H. N., et al. (2017) · Journal of the American Chemical Society · doi:10.1021/jacs.7b07352 · Cited by 66
Epigallocatechin gallate improves insulin resistance in HepG2 cells through alleviating inflammation and lipotoxicity
Zhang, Q., Yuan, H., Zhang, C., Guan, Y., Wu, Y., Ling, F., Niu, Y., & Li, Y. (2018) · Diabetes Research and Clinical Practice · doi:10.1016/j.diabres.2018.06.017 · Cited by 68
Microalgal Biomass and Lipid Production on Dairy Effluent Using a Novel Microalga, Chlorella sp. Isolated from Dairy Wastewater
Choi, Y., Jang, H. M., & Kan, E. (2018) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-018-0094-y · Cited by 68
Simultaneous treatment of domestic wastewater and bio-lipid synthesis using immobilized and suspended cultures of microalgae and activated sludge
Katam, K., & Bhattacharyya, D. (2018) · Journal of Industrial and Engineering Chemistry · doi:10.1016/j.jiec.2018.09.031 · Cited by 65
Optimization of microwave assisted lipid extraction from microalga Scenedesmus obliquus grown on municipal wastewater
Zhou, X., Jin, W., Tu, R., et al. (2019) · Journal of Cleaner Production · doi:10.1016/j.jclepro.2019.02.260 · Cited by 66
Effect of NaCl replacement by other chloride salts on physicochemical parameters, proteolysis and lipolysis of dry-cured foal “cecina”
Cittadini, A., Domínguez, R., Gómez, B., et al. (2019) · Journal of Food Science and Technology · doi:10.1007/s13197-019-04195-6 · Cited by 66
Distinct photo-oxidation-induced cell death pathways lead to selective killing of human breast cancer cells
dos Santos, A. F., Inague, A., Arini, G. S., Terra, L. F., Wailemann, R. A. M., Pimentel, A. C., Yoshinaga, M. Y., Silva, R., Severino, D., de Almeida, D. R. Q., et al. (2020) · Cell Death and Disease · doi:10.1038/s41419-020-03275-2 · Cited by 73

Authors: Bligh, E. G., & Dyer, W. J.
Venue: Canadian Journal of Biochemistry and Physiology
Publisher: Canadian Science Publishing
Published: 1959-08-01
Volume: 37
Issue: 8
Pages: 911-917
ISSN: 0576-5544 (ISSN-L)
DOI: 10.1139/o59-099
OpenAlex: W2152311269
Cited by: 54,751
References: 5
Citations by year: 2026: 1149 · 2025: 1534 · 2024: 1685 · 2023: 1749 · 2022: 1802 · 2021: 2073 · 2020: 2152 · 2019: 1870 · 2018: 1737 · 2017: 1784 · 2016: 1780 · 2015: 1800 · 2014: 1786 · 2013: 1758 · 2012: 1551
FWCI: 8.73
Type: journal article
Language: en
Open access: closed
Licence: www.nrcresearchpress.com/page/about/CorporateTextAndDataMining (tdm)
Locations: Canadian Journal of Biochemistry and Physiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
Keywords: lipid extraction, lipid purification, chloroform-methanol extraction, frozen fish, lipid degradation, fish muscle, tissue homogenization, rapid extraction method
MeSH terms: Fishes, Lipids

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks