In Vivo Delivery of RNAi with Lipid-Based Nanoparticles

A new concept for macromolecular therapeutics in cancer chemotherapy: mechanism of tumoritropic accumulation of proteins and the antitumor agent smancs.

Matsumura, Y., & Maeda, H. (1986) · PubMed · Cited by 6,781

A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine.

Boussif, O., Lezoualc’h, F., Zanta, M. A., Djavaheri‐Mergny, M., Scherman, D., DEMENEIX, B. A., & Behr, J. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.16.7297 · Cited by 6,256

The nuclear RNase III Drosha initiates microRNA processing

Lee, Y., Ahn, C., Han, J., Choi, H., Kim, J., Yim, J., Lee, J., Provost, P., Rådmark, O., Kim, S., et al. (2003) · Nature · doi:10.1038/nature01957 · Cited by 5,299

Role for a bidentate ribonuclease in the initiation step of RNA interference

Bernstein, E., Caudy, A. A., Hammond, S. M., & Hannon, G. J. (2001) · Nature · doi:10.1038/35053110 · Cited by 5,018

Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.

Felgner, P. L., Gadek, T. R., Holm, M., Roman, R., Chan, H. W., Wenz, M., Northrop, J. P., RINGOLD, G. M., & Danielsen, M. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.21.7413 · Cited by 4,954

A System for Stable Expression of Short Interfering RNAs in Mammalian Cells

Brummelkamp, T. R., Bernards, R., & Agami, R. (2002) · Science · doi:10.1126/science.1068999 · Cited by 4,295

Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8

Heil, F., Hemmi, H., Hochrein, H., Ampenberger, F., Kirschning, C., Akira, S., Lipford, G., Wagner, H., & Bauer, S. (2004) · Science · doi:10.1126/science.1093620 · Cited by 3,947

Opsonization, biodistribution, and pharmacokinetics of polymeric nanoparticles

OWENSIII, D., & PEPPAS, N. (2006) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2005.10.010 · Cited by 3,637

Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA

Diebold, S. S., Kaisho, T., Hemmi, H., Akira, S., & Reis e Sousa, C. (2004) · Science · doi:10.1126/science.1093616 · Cited by 3,444

Inhibition of Hedgehog signaling enhances delivery of chemotherapy in a mouse model of pancreatic cancer

Olive, K. P., Jacobetz, M. A., Davidson, C. J., Gopinathan, A., McIntyre, D., Honess, D., Madhu, B., Goldgraben, M. A., Caldwell, M. E., Allard, D., et al. (2009) · Science · doi:10.1126/science.1171362 · Cited by 3,170

An oral dsRNA delivery system based on chitosan induces G protein-coupled receptor kinase 2 gene silencing for Apolygus lucorum control

Qiao, H., Zhao, J., Wang, X., Xiao, L., Zhu‐Salzman, K., Lei, J., Xu, D., Xu, G., Tan, Y., & Hao, D. (2023) · Pesticide Biochemistry and Physiology · doi:10.1016/j.pestbp.2023.105481 · Cited by 26

Nanoparticles for Targeting Intratumoral Hypoxia: Exploiting a Potential Weakness of Glioblastoma

Aldea, M. D., Florian, I. A., Kacsó, G., Craciun, L., Boca, S., Şoriţău, O., & Florian, I. Ş. (2016) · Pharmaceutical Research · doi:10.1007/s11095-016-1947-8 · Cited by 26

Nanotechnology synergized immunoengineering for cancer

Chauhan, D. S., Dhasmana, A., Laskar, P., Prasad, R., Jain, N. K., Srivastava, R., Jaggi, M., Chauhan, S. C., & Yallapu, M. M. (2021) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2021.03.010 · Cited by 27

Hypoxia-Responsive Copolymer for siRNA Delivery

Perche, F., Biswas, S., Patel, N. R., & Torchilin, V. P. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-3148-4_12 · Cited by 25

Optimization of Formulations Consisting of Layered Double Hydroxide Nanoparticles and Small Interfering RNA for Efficient Knockdown of the Target Gene

Wu, Y., Gu, W., Chen, C., Trong, S., & Xu, Z. P. (2018) · ACS Omega · doi:10.1021/acsomega.8b00397 · Cited by 24

Recent advances in characterization of nonviral vectors for delivery of nucleic acids: impact on their biological performance

Oliveira, C. L., Silveira, I., Veiga, F., & Ribeiro, A. J. (2014) · Expert Opinion on Drug Delivery · doi:10.1517/17425247.2014.945421 · Cited by 23

Shell-detachable nanoparticles based on a light-responsive amphiphile for enhanced siRNA delivery

Li, H., Wang, H., Sun, C., Du, J., & Wang, J. (2013) · RSC Advances · doi:10.1039/c3ra44866e · Cited by 23

Lung-specific supramolecular nanoparticles for efficient delivery of therapeutic proteins and genome editing nucleases

Liu, J., Zheng, Q., Yao, R., & Wang, M. (2024) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2406654121 · Cited by 22

Lipid-Coated Calcium Phosphate Nanoparticles for Nonviral Gene Therapy

Haynes, M. T., & Huang, L. (2014) · Advances in genetics · doi:10.1016/b978-0-12-800148-6.00007-9 · Cited by 21

Stimuli-responsive polymeric micelles for extracellular and intracellular drug delivery

Sarısözen, C., Joshi, U., Mendes, L. P., & Torchilin, V. P. (2019) · Elsevier eBooks · doi:10.1016/b978-0-08-101995-5.00012-x · Cited by 22

Anticancer siRNA delivery by new anticancer molecule: A novel combination strategy for cancer cell killing

Muktapuram, P. R., Gara, R. K., Sharma, K., Rohit, C., Srinivas, K., Mishra, D. P., & Bathula, S. R. (2012) · European Journal of Medicinal Chemistry · doi:10.1016/j.ejmech.2012.07.035 · Cited by 20

Covalent conjugation of oligonucleotides with cell-targeting ligands

Alam, M. R., Ming, X., Nakagawa, O., Jin, J., & Juliano, R. L. (2013) · Bioorganic & Medicinal Chemistry · doi:10.1016/j.bmc.2013.05.037 · Cited by 19

Antisense and Functional Nucleic Acids in Rational Drug Development

Prof. Robert Penchovsky, D. R. N., Georgieva, A. V., Dyakova, V., Traykovska, M., & Pavlova, N. (2024) · Antibiotics · doi:10.3390/antibiotics13030221 · Cited by 19

Maximizing the Supported Bilayer Phenomenon: Liposomes Comprised Exclusively of PEGylated Phospholipids for Enhanced Systemic and Lymphatic Delivery

Haynes, M. T., & Huang, L. (2016) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.6b05534 · Cited by 19

Nanoparticle Based Gene Therapy Approach: A Pioneering Rebellion in the Management of Psychiatric Disorders

Rehman, S., Nabi, B., Pottoo, F. H., Baboota, S., & Ali, J. (2020) · Current Gene Therapy · doi:10.2174/1566523220666200607185903 · Cited by 20

Shedding PEG Palisade by Temporal Photostimulation and Intracellular Reducing Milieu for Facilitated Intracellular Trafficking and DNA Release

Wang, T., Chen, Q., Lu, H., Li, W., Li, Z., Ma, J., & Gao, H. (2016) · Bioconjugate Chemistry · doi:10.1021/acs.bioconjchem.6b00355 · Cited by 17

Engineering nanocages with polyglutamate domains for coupling to hydroxyapatite biomaterials and allograft bone

Culpepper, B. K., Morris, D. S., Prevelige, P. E., & Bellis, S. L. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2012.12.026 · Cited by 16

Anti-EGFR immunonanoparticles containing IL12 and salmosin genes for targeted cancer gene therapy

Kim, J. S., Kang, S. J., Jeong, H. Y., Kim, M. W., Park, S. I., Lee, Y. K., Kim, H. S., Kim, K. S., & Park, Y. S. (2016) · International Journal of Oncology · doi:10.3892/ijo.2016.3619 · Cited by 16

Conjugation Approaches for Peptide-Mediated Delivery of Oligonucleotides Therapeutics

Patil, N. A. (2021) · Australian Journal of Chemistry · doi:10.1071/ch21131 · Cited by 15

Gel Phase 1,2-Distearoyl-sn-glycero-3-phosphocholine-Based Liposomes Are Superior to Fluid Phase Liposomes at Augmenting Both Antigen Presentation on Major Histocompatibility Complex Class II and Costimulatory Molecule Display by Dendritic Cells in Vitro

Norling, K., Bernasconi, V., Hernández, V. A., Parveen, N., Edwards, K., Lycke, N. Y., Höök, F., & Bally, M. (2019) · ACS Infectious Diseases · doi:10.1021/acsinfecdis.9b00189 · Cited by 14

PEG-detachable lipid–polymer hybrid nanoparticle for delivery of chemotherapy drugs to cancer cells

Du, J., Song, Y., Ye, W., Cheng, Y., Cui, H., Liu, D., Liu, M., Zhang, B., & Zhou, S. (2014) · Anti-Cancer Drugs · doi:10.1097/cad.0000000000000092 · Cited by 13

Multistage Delivery Technologies: Multifunctional, Interdisciplinary Approaches to Nanomedicine

Haynes, M. T., & Huang, L. (2016) · Molecular Therapy · doi:10.1038/mt.2016.75 · Cited by 13

Efficient in vitro delivery of Noggin siRNA enhances osteoblastogenesis

Ghadakzadeh, S., Hamdy, R. C., & Tabrizian, M. (2017) · Heliyon · doi:10.1016/j.heliyon.2017.e00450 · Cited by 12

Focused Delivery of Chemotherapy to Augment Surgical Management of Brain Tumors

Mehkri, Y., Woodford, S. J., Pierre, K., Dagra, A., Hernandez, J. S., Siyanaki, M. R. H., Azab, M. A., & Lucke-Wold, B. P. (2022) · Current Oncology · doi:10.3390/curroncol29110696 · Cited by 12

Preparation and Characterization of siRNA-Loaded Liposomes

Liu, Y., & Huang, L. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1298-9_10 · Cited by 11

Salt-Induced Stability and Serum-Resistance of Polyglutamate Polyelectrolyte Brushes/Nuclear Factor-κB p65 siRNA Polyplex Enhance the Apoptosis and Efficacy of Doxorubicin

Zhao, Y., Qin, Y., Liang, Y., Zou, H., Peng, X., Huang, H., Lü, M., & Feng, M. (2013) · Biomacromolecules · doi:10.1021/bm400177q · Cited by 10

Development of Antisense Oligonucleotide Gapmers for the Treatment of Dyslipidemia and Lipodystrophy

Aslesh, T., & Yokota, T. (2020) · Methods in molecular biology · doi:10.1007/978-1-0716-0771-8_5 · Cited by 10

Hepatic RNA interference: delivery by synthetic vectors

Haynes, M. T., & Huang, L. (2013) · Drug Delivery and Translational Research · doi:10.1007/s13346-013-0157-8 · Cited by 9

Development of Nanoparticles Incorporating a Novel Liposomal Membrane Destabilization Peptide for Efficient Release of Cargos into Cancer Cells

Itakura, S., Hama, S., Ohgita, T., & Kogure, K. (2014) · PLoS ONE · doi:10.1371/journal.pone.0111181 · Cited by 9

The role of circular RNA during the urological cancer metastasis: exploring regulatory mechanisms and potential therapeutic targets

Xu, Y., Gao, Z., Sun, X., Li, J., Ozaki, T., Shi, D., Yu, M., & Zhu, Y. (2024) · Cancer and Metastasis Reviews · doi:10.1007/s10555-024-10182-x · Cited by 8

Stimuli‐Sensitive Polymeric Nanomedicines for Cancer Imaging and Therapy

Perche, F., Biswas, S., & Torchilin, V. P. (2015) · Wiley eBooks · doi:10.1002/9781119041412.ch11 · Cited by 8

Advances in Therapeutic Approaches Using RNA Interference as a Gene Silencing Tool

Kaymaz, B. T., & Kosov, B. (2013) · Advanced Techniques in Biology & Medicine · doi:10.4172/2379-1764.1000108 · Cited by 7

Nanolipoparticles-mediated MDR1 siRNA delivery: preparation, characterization and cellular uptake

Nourbakhsh, M., Behravan, J., Lage, H., Abnous, K., Mosaffa, F., Badiee, A., & Jaafari, M. R. (2015) · DOAJ (DOAJ: Directory of Open Access Journals) · Cited by 7

Gene therapy strategies in glaucoma and application for steroid-induced hypertension

Borrás, T. (2011) · Saudi Journal of Ophthalmology · doi:10.1016/j.sjopt.2011.07.005 · Cited by 6

Fabrication of A Folic Acid-Modified Arsenic Trioxide Prodrug Liposome and Assessment of its Anti-Hepatocellular Carcinoma Activity

Jing-Jing, Z., Chen, X., Wen-Dong, Y., Wei, Y., Zheng, H., Zheng, H., Zhi-Hong, Z., Wang, B., & Li, F. (2020) · Digital Chinese Medicine · doi:10.1016/j.dcmed.2020.12.005 · Cited by 6

Pentetic acid derived dual purpose fluorescent carbon dots for pH responsive drug delivery and antioxidant proficiency

Hussain, A., Alajmi, M. F., & Ganguly, S. (2024) · Diamond and Related Materials · doi:10.1016/j.diamond.2024.111101 · Cited by 5

Insight into the preparation of biopolymeric nanoparticles with lithium and their cellular uptake studies using PC 12 cells

Geeva, & Narayan, S. (2022) · Materials Research Innovations · doi:10.1080/14328917.2021.2023288 · Cited by 5

Applicative Use of Electroporation Models

Rems, L. (2017) · Advances in biomembranes and lipid self-assembly · doi:10.1016/bs.abl.2017.06.001 · Cited by 5

A pH‐Responsive Charge‐Convertible Drug Delivery Nanocarrier for Precise Starvation and Chemo Synergistic Oncotherapy

Zhou, Y., Gao, X., Lu, Y., Zhang, R., Lv, K., Gong, J., Feng, J., & Zhang, H. (2022) · ChemPlusChem · doi:10.1002/cplu.202200394 · Cited by 5

Lipids as Biological Materials for Nanoparticulate Delivery

Kaur, I. P., Bhandari, R., & Yakhmi, J. V. (2014) · Springer eBooks · doi:10.1007/978-3-642-31107-9_27 · Cited by 5

The “Non-viral” Approach for siRNA Delivery in Cancer Treatment: A Special Focus on Micelles and Liposomes

Navarro, G., Essex, S., & Torchilin, V. P. (2013) · RNA technologies · doi:10.1007/978-3-642-36853-0_10 · Cited by 4

Containers Based Drug Delivery for Neuroscience

Jose, D. A., Krishnapriya, & Chakrapani, P. S. B. (2022) · Composites science and technology · doi:10.1007/978-981-16-8146-2_14 · Cited by 4

Mass Spectrometry-Assisted High-Throughput Discovery of Lung-Specific Lipid Nanoparticles for In Vivo Protein Delivery

Liu, J., Zhang, Y., Yao, R., Ma, T., Zhao, Z., & Wang, M. (2026) · Nano Letters · doi:10.1021/acs.nanolett.6c00040 · Cited by 3

Iron Oxide Nanoparticles Application in Toxicity Therapeutics of CNS Disorders Indicated by Molecular MRI

Zhao, L., Peng, T., & Wu, S. (2025) · Nanotechnology and nanomaterials. · doi:10.5772/intechopen.1008681 · Cited by 3

Silencing of BCSG1 with specific siRNA via nanocarriers for breast cancer treatment

Yang, C., Cheng, X., & Shen, P. (2021) · Bulletin du Cancer · doi:10.1016/j.bulcan.2020.10.022 · Cited by 3

Role of miRNAs in the Response to Anticancer Therapy

Frampton, A. E., Krell, J., Giovannetti, E., Jiao, L. R., & Stebbing, J. (2012) · Pharmacogenomics · doi:10.2217/pgs.12.34 · Cited by 2

Concluding remarks and future perspective of combination drug delivery systems

Nasser, T. A., AbdElhamid, A. S., Teleb, M., Khattab, S. N., Bekhit, A. A., Elkhodairy, K. A., & Elzoghby, A. O. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-85873-1.00004-6 · Cited by 2

A New Approach to Cancer Therapy: The Tumor Microenvironment as Target

Schwendener, R. A., & Mete, S. (2013) · BENTHAM SCIENCE PUBLISHERS eBooks · doi:10.2174/9781608057986113010003 · Cited by 2

The “Non-viral” Approach for siRNA Delivery in Cancer Treatment: A Special Focus on Micelles and Liposomes

Navarro, G., Essex, S., & Torchilin, V. P. (2013) · Springer eBooks · doi:10.1007/978-3-662-45775-7_10 · Cited by 2

Cancer Therapy with RNAi Delivered by Non‐viral Membrane/Core Nanoparticles

Satterlee, A., & Huang, L. (2014) · doi:10.1002/9781118501665.ch4 · Cited by 2

The Tumor Microenvironment as Target for New Cancer Therapies

Schwendener, R. A., & Mete, S. (2014) · Cited by 1

Systemic delivery of phosphorylated nucleoside analogues and siRNA via LCP nanoparticles for cancer therapy

Zhang, Y. (2019) · Carolina Digital Repository (University of North Carolina at Chapel Hill) · doi:10.17615/d2mb-8610 · Cited by 1

RNAi in Liver Diseases

Li, J., Lu, J., Zhang, Y., Ghazwani, M., Zhang, P., Gao, X., & Li, S. (2013) · doi:10.1002/9781118610749.ch15 · Cited by 1

Chemical vectors for gene delivery

Xu, Z., & Huang, L. (2013) · doi:10.2217/ebo.12.304 · Cited by 1

Polyurethanes: Surface Protein Adsorption

Kürkçüoğlu, Ö., & Güner, F. S. (2016) · Taylor & Francis eBooks · doi:10.1081/e-ebpp-120050029 · Cited by 1

Desenvolvimento e caracterização de sistemas de liberação tópica a base de cristais líquidos com vitamina E TPGS para veiculação de siRNA na terapia gênica

Mano, D. D. M. (2012) · doi:10.11606/d.60.2012.tde-27062013-093226 · Cited by 1

Desenvolvimento e caracterização de sistemas de liberação tópica a base de cristais líquidos para veiculação de siRNA na terapia gênica

Depieri, L. V. (2012) · doi:10.11606/d.60.2012.tde-27062012-162117

Analysis of siRNA Delivery Using Various Methodologies

Pei, Y. (2013) · Wiley eBooks · doi:10.1002/9781118610749.ch2

Gene Therapy: Technologies and Applications

Morishita, R., & Nakagami, H. (2013)

Core-Shell Nanoparticles for Biomedical Applications

Elsabahy, M., & Wooley, K. L. (2014) · Frontiers in nanobiomedical research · doi:10.1142/9789814520652_0013

Nanodispersões de fase líquido-cristalina como carreadoras de siRNA no tratamento tópico da psoríase: Avaliação em modelos in vitro e in vivo

Depieri, L. V. (2016) · doi:10.11606/t.60.2016.tde-05102016-093901

Avaliação in vitro e in vivo de nanodispersões líquido cristalinas para veiculação de siRNA-TRP-1 para o tratamento tópico do vitiligo

Tofani, L. B. (2015) · doi:10.11606/d.60.2015.tde-05112015-103546

Investigating the functional roles of microRNA-29b and microRNA-146a in prion diseases

Zhao, W. (2018) · Minerva Access (University of Melbourne)

Synthesis of C4'-CF3-α-L-Deoxythymidine Phosphoramidite

周, 逸. (2019) · Hans Journal of Chemical Engineering and Technology · doi:10.12677/hjcet.2019.92020

Introduction

Obulesu, M. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-819882-7.00001-5

Nanopartículas híbridas polímero-lipídicas associadas à internalização fotoquímica para liberação de siRNA na terapia tópica da psoríase

Suzuki, I. (2020) · doi:10.11606/t.60.2020.tde-30092021-161929

Frontiers in Clinical Drug Research - Anti-Cancer Agents

Schwendener, R. A., & Mete, S. (2013) · BENTHAM SCIENCE PUBLISHERS eBooks · doi:10.2174/97816080579861130101

Regulation of the Intrahepatic CD8+ T Lymphocyte

Dolina, J. S. (2013) · doi:10.18130/v3s806

Targeted delivery of therapeutic oligonucleotides in pancreatic cancer

Iris, Y. (2022) · Journal of Biology and Medicine · doi:10.17352/jbm.000028

Nanoparticles for Diagnosis and Treatment of Infectious Diseases

Mukherjee, S., Pal, D., Bhattacharyya, A., Mondal, P., & Roy, S. (2024) · Lecture notes in nanoscale science and technology · doi:10.1007/978-3-031-57843-4_4

Advancements in Drug Delivery Methods for the Treatment of Brain Disease

Devarapalli, J. B. (2025) · World Journal of Current Medical and Pharmaceutical Research · doi:10.37022/wjcmpr.v7i2.356

Revolutionizing lung cancer immunotherapy: Small interfering RNA-based gene silencing for immune checkpoint blockade

Saha, R., Kumar, S., Singh, R., Attri, R., & Kumar, B. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-44585-9.00011-1

Development of Antisense Oligonucleotide Gapmers for the Treatment of Dyslipidemia and Lipodystrophy

Aslesh, T., & Yokota, T. (2026) · Methods in molecular biology · doi:10.1007/978-1-0716-5476-7_5

Research and application progress of siRNA nano-delivery systems in gliomas

He, M., Sun, F., Wu, L., Gao, Y., Wang, W., Dong, J., & Yan, J. (2026) · Journal of drug targeting · doi:10.1080/1061186x.2026.2756480

Lipid-based nanocarriers for RNA delivery

(2015) · TUScholarShare (Temple University) · doi:10.34944/dspace/5203

Nanoparticle-Based Delivery of RNAi Therapeutics: Progress and Challenges

Zhou, J., Shum, K., Burnett, J., & Rossi, J. J. (2013) · Pharmaceuticals · doi:10.3390/ph6010085 · Cited by 211

Delivery of RNAi therapeutics: work in progress

Miller, A. D. (2013) · Expert Review of Medical Devices · doi:10.1586/17434440.2013.855471 · Cited by 34

Navigating the intricate in-vivo journey of lipid nanoparticles tailored for the targeted delivery of RNA therapeutics: a quality-by-design approach

Haghighi, E., Abolmaali, S. S., Dehshahri, A., Shaegh, S. A. M., Azarpira, N., & Tamaddon, A. M. (2024) · Journal of Nanobiotechnology · doi:10.1186/s12951-024-02972-w · Cited by 125

Non-viral nanoparticles for RNA interference: Principles of design and practical guidelines

Liu, Z., Wang, S., Tapeinos, C., Torrieri, G., Känkänen, V., El‐Sayed, N., Python, A., Hirvonen, J. J., & Santos, H. A. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.05.018 · Cited by 73