Cited by 140Cellular Mechanics and InteractionsForce Microscopy Techniques and ApplicationsMicrotubule and mitosis dynamics

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Mechanotransduction by the Actin Cytoskeleton: Converting Mechanical Stimuli into Biochemical Signals

Harris, Andrew R., Jreij, Pamela, Fletcher, Daniel A.·2018·Annual Review of Biophysics

DOI 10.1146/annurev-biophys-070816-033547

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Abstract

Force transmission through the actin cytoskeleton plays a central role in cell movements, shape change, and internal organization. Dynamic reorganization of actin filaments by an array of specialized binding proteins creates biochemically and architecturally distinct structures, many of which are finely tuned to exert or resist mechanical loads. The molecular complexity of the actin cytoskeleton continues to be revealed by detailed biochemical assays, and the architectural diversity and dynamics of actin structures are being uncovered by advances in super-resolution fluorescence microscopy and electron microscopy. However, our understanding of how mechanical forces feed back on cytoskeletal architecture and actin-binding protein organization is comparatively limited. In this review, we discuss recent work investigating how mechanical forces applied to cytoskeletal proteins are transduced into biochemical signals. We explore multiple mechanisms for mechanical signal transduction, including the mechanosensitive behavior of actin-binding proteins, the effect of mechanical force on actin filament dynamics, and the influence of mechanical forces on the structure of single actin filaments. The emerging picture is one in which the actin cytoskeleton is defined not only by the set of proteins that constitute a network but also by the constant interplay of mechanical forces and biochemistry.

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“It has become apparent that this coordination emerges largely as a result of deep interactions between biochemical signaling, cytoskeletal dynamics, and forces, each having been shown to modulate the other two (Hannezo and Heisenberg, 2019; Harris et al., 2018).”
Background
Insight into Mechanobiology: How Stem Cells Feel Mechanical Forces and Orchestrate Biological Functions
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See all 140

Authors: Harris, A. R., Jreij, P., & Fletcher, D. A.
Affiliations: University of California, Berkeley (US)
Lawrence Berkeley National Laboratory (US)
ORCID: Harris, Andrew R.
Fletcher, Daniel A.
Venue: Annual Review of Biophysics
Publisher: Annual Reviews
Published: 2018-05-20
Volume: 47
Issue: 1
Pages: 617-631
ISSN: 1936-122X (ISSN-L)
1936-1238
DOI: 10.1146/annurev-biophys-070816-033547
OpenAlex: W2803134496
Cited by: 140
References: 101
Citations by year: 2026: 10 · 2025: 16 · 2024: 19 · 2023: 14 · 2022: 14 · 2021: 24 · 2020: 25 · 2019: 15 · 2018: 3
FWCI: 1.88
Type: journal article
Language: en
Open access: closed
Locations: Annual Review of Biophysics (publishedVersion)
Funders: National Institutes of Health
National Institute of General Medical Sciences
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords: mechanotransduction, actin cytoskeleton, mechanical forces, actin-binding proteins, actin filament dynamics, actin filament structure, force transmission, mechanical signal transduction, cytoskeletal structure, cell mechanics

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Mechanotransduction by the Actin Cytoskeleton: Converting Mechanical Stimuli into Biochemical Signals

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