The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Fluorescence Correlation Spectroscopy: Novel Variations of an Established Technique
Abstract
Fluorescence correlation spectroscopy (FCS) is one of the major biophysical techniques used for unraveling molecular interactions in vitro and in vivo. It allows minimally invasive study of dynamic processes in biological specimens with extremely high temporal and spatial resolution. By recording and correlating the fluorescence fluctuations of single labeled molecules through the exciting laser beam, FCS gives information on molecular mobility and photophysical and photochemical reactions. By using dual-color fluorescence cross-correlation, highly specific binding studies can be performed. These have been extended to four reaction partners accessible by multicolor applications. Alternative detection schemes shift accessible time frames to slower processes (e.g., scanning FCS) or higher concentrations (e.g., TIR-FCS). Despite its long tradition, FCS is by no means dated. Rather, it has proven to be a highly versatile technique that can easily be adapted to solve specific biological questions, and it continues to find exciting applications in biology and medicine.
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Water-Soluble Quantum Dots for Multiphoton Fluorescence Imaging in Vivo
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Thermodynamic Fluctuations in a Reacting System—Measurement by Fluorescence Correlation Spectroscopy
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Fluorescence correlation spectroscopy. II. An experimental realization
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Self-citationFluorescence Correlation Spectroscopy
(2011) · SpringerReference · doi:10.1007/springerreference_33960 · Cited by 714
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Single Particle Tracking: From Theory to Biophysical Applications
Shen, H., Tauzin, L. J., Baiyasi, R., Wang, W., Moringo, N., Shuang, B., & Landes, C. F. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00815 · Cited by 536
Accurate Single-Molecule FRET Studies Using Multiparameter Fluorescence Detection
Sisamakis, E., Valeri, A., Kalinin, S., Rothwell, P. J., & Seidel, C. A. M. (2010) · Methods in Enzymology · doi:10.1016/s0076-6879(10)75018-7 · Cited by 268
PINK1 drives Parkin self-association and HECT-like E3 activity upstream of mitochondrial binding
Lazarou, M., Narendra, D. P., Jin, S. M., Tekle, E., Banerjee, S., & Youle, R. J. (2013) · Journal of Cell Biology · doi:10.1083/jcb.201210111 · Cited by 236
Mirbaha, H., Holmes, B. B., Sanders, D. W., Bieschke, J., & Diamond, M. I. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.652693 · Cited by 215
Golebiewska, U., Nyako, M., Woturski, W., Zaitseva, I., & McLaughlin, S. (2008) · Molecular Biology of the Cell · doi:10.1091/mbc.e07-12-1208 · Cited by 140
Metadata
- Authors
- Haustein, E., & Schwille, P.
- Venue
- Annual Review of Biophysics and Biomolecular Structure
- Publisher
- Annual Reviews
- Published
- 2007-06
- Volume
- 36
- Issue
- 1
- Pages
- 151-169
- ISSN
- 1056-8700 (ISSN-L)
- 1545-4266
- OpenAlex
- W2115191599
- Cited by
- 548
- References
- 49
- FWCI
- 21.05
- Type
- journal article
- Language
- en
- Open access
- closed
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- Haustein, E., & Schwille, P. (2007). Fluorescence Correlation Spectroscopy: Novel Variations of an Established Technique. Annual Review of Biophysics and Biomolecular Structure. https://doi.org/10.1146/annurev.biophys.36.040306.132612
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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