The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Single-Molecule Approach to Molecular Biology in Living Bacterial Cells
Abstract
Recent developments on fluorescent proteins and microscopy techniques have allowed the probing of single molecules in a living bacterial cell with high specificity, millisecond time resolution, and nanometer spatial precision. Recording movies and analyzing dynamics of individual macromolecules have brought new insights into the mechanisms of many processes in molecular biology, such as DNA-protein interactions, gene regulation, transcription, translation, and replication, among others. Here we review the key methods of single-molecule detection and highlight numerous examples to illustrate how these experiments are contributing to the quantitative understanding of the fundamental processes in a living cell.
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Citations
Cites
Stochastic Gene Expression in a Single Cell
Elowitz, M. B., Levine, A. J., Siggia, E. D., & Swain, P. S. (2002) · Science · doi:10.1126/science.1070919 · Cited by 5,441
Precise Nanometer Localization Analysis for Individual Fluorescent Probes
Thompson, R. E., Larson, D. R., & Webb, W. W. (2002) · Biophysical Journal · doi:10.1016/s0006-3495(02)75618-x · Cited by 2,320
Stochastic protein expression in individual cells at the single molecule level
Cai, L., Friedman, N., & Xie, X. S. (2006) · Nature · doi:10.1038/nature04599 · Cited by 1,145 · cited 5×
“(15) developed an assay to circumvent the loss of signal due to the efflux problem: A live cell is trapped in a polydimethylsiloxane (PDMS)based microfluidic chamber in a small volume that confines and allows rapid mixing of the expelled fluorophores.”
BackgroundMethodInfluentialSelf-citationProbing Gene Expression in Live Cells, One Protein Molecule at a Time
Yu, J., Xiao, J., Ren, X., Lao, K., & Xie, X. S. (2006) · Science · doi:10.1126/science.1119623 · Cited by 918
Self-citationENZYME INDUCTION AS AN ALL-OR-NONE PHENOMENON
Novick, A., & Weiner, M. (1957) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.43.7.553 · Cited by 891
Linking Stochastic Dynamics to Population Distribution: An Analytical Framework of Gene Expression
Friedman, N., Cai, L., & Xie, X. S. (2006) · Physical Review Letters · doi:10.1103/physrevlett.97.168302 · Cited by 700
Self-citation
Cited by
Metzler, R., Jeon, J.-H., Cherstvy, A. G., & Barkai, E. (2014) · Phys. Chem. Chem. Phys. · doi:10.1039/c4cp03465a · Cited by 1,765
A Stochastic Single-Molecule Event Triggers Phenotype Switching of a Bacterial Cell
Choi, P. J., Cai, L., Frieda, K., & Xie, X. S. (2008) · Science · doi:10.1126/science.1161427 · Cited by 438
Self-citationUltra-stable organic fluorophores for single-molecule research
Zheng, Q., Juette, M. F., Jockusch, S., Wasserman, M. R., Zhou, Z., Altman, R. B., & Blanchard, S. C. (2014) · Chem. Soc. Rev. · doi:10.1039/c3cs60237k · Cited by 406
Single-Molecule Studies of the Neuronal SNARE Fusion Machinery
Brunger, A. T., Weninger, K., Bowen, M., & Chu, S. (2009) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.77.070306.103621 · Cited by 107
Kinetic modeling of the genetic information processes in a minimal cell
Thornburg, Z. R., Melo, M. C. R., Bianchi, D., Brier, T. A., Crotty, C., Breuer, M., Smith, H. O., Hutchison, C. A., Glass, J. I., & Luthey-Schulten, Z. (2019) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2019.00130 · Cited by 22
Metadata
- Authors
- Xie, X. S., Choi, P. J., Li, G.-W., Lee, N. K., & Lia, G.
- Venue
- Annual Review of Biophysics
- Publisher
- Annual Reviews
- Published
- 2008-06-01
- Volume
- 37
- Issue
- 1
- Pages
- 417-444
- ISSN
- 1936-122X (ISSN-L)
- 1936-1238
- OpenAlex
- W2156302599
- Cited by
- 351
- References
- 6
- FWCI
- 1.84
- Type
- journal article
- Language
- en
- Open access
- closed
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- Xie, X. S., Choi, P. J., Li, G.-W., Lee, N. K., & Lia, G. (2008). Single-Molecule Approach to Molecular Biology in Living Bacterial Cells. Annual Review of Biophysics. https://doi.org/10.1146/annurev.biophys.37.092607.174640
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
